(Circular) (Six-base) MAPSORT of: pFN21AE1847.seq Check: 3194 from: 1 to: 5800 pFN21AE1847 5800 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2661 3143 1937 Size: 724 482 4594 Fragments arranged by size: 4594 724 482 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3863 5247 5515 1632 Size: 2231 1384 268 1917 Fragments arranged by size: 2231 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 4699 5676 1203 Size: 3496 977 1327 Fragments arranged by size: 3496 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 4529 4529 Size: 5800 AleI CACnn'nnGTG Cuts at: 1166 2999 1166 Size: 1833 3967 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2977 1184 Size: 1793 4007 AlwNI CAG_nnn'CTG Cuts at: 3543 5115 3543 Size: 1572 4228 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 3109 3335 3487 5596 1493 Size: 1616 226 152 2109 1697 Fragments arranged by size: 2109 1697 1616 226 152 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 3125 1870 Size: 73 1182 4545 Fragments arranged by size: 4545 1182 73 BamHI G'GATC_C Cuts at: 3130 3497 3130 Size: 367 5433 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2222 2315 2351 3121 4576 Size: 325 217 627 435 93 36 770 1455 Cuts at: 4576 618 Size: 1842 Fragments arranged by size: 1842 1455 770 627 435 325 217 93 36 BanII G_rGCy'C Cuts at: 1391 2086 3119 1391 Size: 695 1033 4072 Fragments arranged by size: 4072 1033 695 BbeI G_GCGC'C Cuts at: 2226 2319 2226 Size: 93 5707 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2715 928 Size: 971 816 4013 Fragments arranged by size: 4013 971 816 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3991 4025 1903 Size: 34 2054 34 3678 Fragments arranged by size: 3678 2054 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2783 3692 4902 2783 Size: 909 1210 3681 Fragments arranged by size: 3681 1210 909 BclI T'GATC_A Cuts at: 1548 2283 1548 Size: 735 5065 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4411 136 Size: 107 122 71 631 605 2739 1525 Fragments arranged by size: 2739 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5795 5795 Size: 5800 BlpI GC'TnA_GC Cuts at: 2342 2476 3217 2342 Size: 134 741 4925 Fragments arranged by size: 4925 741 134 Bme1580I G_kGCm'C Cuts at: 1790 3861 5017 1790 Size: 2071 1156 2573 Fragments arranged by size: 2573 2071 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2506 2912 3531 Size: 416 53 83 399 24 1089 406 619 Cuts at: 3531 4489 442 Size: 958 1753 Fragments arranged by size: 1753 1089 958 619 416 406 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4460 1239 Size: 3221 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3925 4790 5088 3925 Size: 865 298 4637 Fragments arranged by size: 4637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4463 882 Size: 617 2964 2219 Fragments arranged by size: 2964 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3013 3591 493 Size: 711 1809 578 2702 Fragments arranged by size: 2702 1809 711 578 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2223 2316 3607 3989 Size: 53 83 186 936 690 93 1291 382 Cuts at: 3989 4624 275 Size: 635 1451 Fragments arranged by size: 1451 1291 936 690 635 382 186 93 83 53 BsaWI w'CCGG_w Cuts at: 4233 4631 4905 5052 5463 4233 Size: 398 274 147 411 4570 Fragments arranged by size: 4570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5575 Fragments arranged by size: 5575 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2960 1186 Size: 1774 4026 BseYI C'CCAG_C Cuts at: 2137 2230 5003 5499 2137 Size: 93 2773 496 2438 Fragments arranged by size: 2773 2438 496 93 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2445 2973 1163 Size: 573 709 528 3990 Fragments arranged by size: 3990 709 573 528 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3250 4011 5039 1540 Size: 275 186 1249 761 1028 2301 Fragments arranged by size: 2301 1249 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3861 3946 5017 3861 Size: 85 1071 4644 Fragments arranged by size: 4644 1071 85 BsmI GAATG_Cn' Cuts at: 2235 2401 2936 3311 3404 2235 Size: 166 535 375 93 4631 Fragments arranged by size: 4631 535 375 166 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 3119 3861 3946 5017 1391 Size: 399 296 1033 742 85 1071 2174 Fragments arranged by size: 2174 1071 1033 742 399 296 85 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2208 3155 844 Size: 1364 947 3489 Fragments arranged by size: 3489 1364 947 BsrBI CCG'CTC Cuts at: 1394 1823 3687 1394 Size: 429 1864 3507 Fragments arranged by size: 3507 1864 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2109 2610 4295 4469 65 Size: 1183 861 501 1685 174 1396 Fragments arranged by size: 1685 1396 1183 861 501 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4444 4631 1700 Size: 110 2634 187 2869 Fragments arranged by size: 2869 2634 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 2043 1925 Size: 118 5682 BssSI C'ACGA_G Cuts at: 2063 2264 3860 4872 2063 Size: 201 1596 1012 2991 Fragments arranged by size: 2991 1596 1012 201 BstAPI GCAn_nnn'nTGC Cuts at: 794 2931 794 Size: 2137 3663 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstYI r'GATC_y Cuts at: 1972 3130 3497 3882 3899 5351 5437 5449 5795 Size: 1158 367 385 17 1452 86 12 346 Cuts at: 5795 1972 Size: 1977 Fragments arranged by size: 1977 1452 1158 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2574 2674 513 Size: 552 939 570 100 3639 Fragments arranged by size: 3639 939 570 552 100 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5061 Fragments arranged by size: 5061 683 56 BtsI GCAGTG_nn' Cuts at: 2428 3316 4110 4130 2428 Size: 888 794 20 4098 Fragments arranged by size: 4098 888 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4807 809 Size: 507 3491 1802 Fragments arranged by size: 3491 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2740 2980 3247 Size: 54 1425 50 275 18 910 240 267 Cuts at: 3247 4136 5765 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 910 889 275 267 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3247 1537 Size: 275 1435 4090 Fragments arranged by size: 4090 1435 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3730 1353 Size: 2377 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4387 4761 4907 387 Size: 1064 138 2798 374 146 1280 Fragments arranged by size: 2798 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3863 4460 5247 5515 1239 Size: 393 2231 597 787 268 1524 Fragments arranged by size: 2231 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3109 3487 3109 Size: 378 5422 EcoRV GAT'ATC Cuts at: 2781 2781 Size: 5800 FspI TGC'GCA Cuts at: 2807 4306 2807 Size: 1499 4301 HaeII r_GCGC'y Cuts at: 1678 2021 2226 2319 4947 1678 Size: 343 205 93 2628 2531 Fragments arranged by size: 2628 2531 343 205 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4441 4473 4515 4547 1373 Size: 32 135 32 2869 32 42 32 2626 Fragments arranged by size: 2869 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3144 3390 669 Size: 988 281 1206 246 3079 Fragments arranged by size: 3079 1206 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KasI G'GCGC_C Cuts at: 2222 2315 2222 Size: 93 5707 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4914 5098 1305 Size: 3609 184 2007 Fragments arranged by size: 3609 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2742 10 Size: 54 1425 343 910 3068 Fragments arranged by size: 3068 1425 910 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2619 2847 2999 3758 4117 4276 518 Size: 648 1453 228 152 759 359 159 2042 Fragments arranged by size: 2042 1453 759 648 359 228 159 152 MspA1I CmG'CkG Cuts at: 1606 2158 2349 2670 3216 3893 5041 5286 1606 Size: 552 191 321 546 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 552 546 321 245 191 NarI GG'CG_CC Cuts at: 2223 2316 2223 Size: 93 5707 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4309 Fragments arranged by size: 4309 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 2533 2709 3158 4703 1132 Size: 97 1304 176 449 1545 2229 Fragments arranged by size: 2229 1545 1304 449 176 97 PasI CC'CwG_GG Cuts at: 1348 2912 1348 Size: 1564 4236 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5800 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PmlI CAC'GTG Cuts at: 3013 3013 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2459 2491 3017 3049 3869 3901 5382 5414 2459 Size: 32 526 32 820 32 1481 32 2845 Fragments arranged by size: 2845 1481 820 526 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3083 1613 Size: 1470 4330 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PstI C_TGCA'G Cuts at: 830 2392 3152 830 Size: 1562 760 3478 Fragments arranged by size: 3478 1562 760 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 2595 4048 5760 2595 Size: 1453 1712 2635 Fragments arranged by size: 2635 1712 1453 SexAI A'CCwGG_T Cuts at: 2411 2411 Size: 5800 SfcI C'TryA_G Cuts at: 826 1045 2388 3148 4283 4964 5155 826 Size: 219 1343 760 1135 681 191 1471 Fragments arranged by size: 1471 1343 1135 760 681 219 191 SfoI GGC'GCC Cuts at: 2224 2317 2224 Size: 93 5707 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 3127 1872 Size: 1255 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3904 4805 5067 820 Size: 197 926 1961 901 262 1553 Fragments arranged by size: 1961 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 2394 1517 Size: 877 4923 StyI C'CwwG_G Cuts at: 513 1065 2004 2086 3239 513 Size: 552 939 82 1153 3074 Fragments arranged by size: 3074 1153 939 552 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3011 3827 3844 3997 4182 4627 325 Size: 1255 1431 816 17 153 185 445 1498 Fragments arranged by size: 1498 1431 1255 816 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2593 4046 5758 Size: 274 80 33 51 1443 616 1453 1712 Cuts at: 5758 96 Size: 138 Fragments arranged by size: 1712 1453 1443 616 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2666 2698 5760 5792 2666 Size: 32 3062 32 2674 Fragments arranged by size: 3062 2674 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2413 1316 Size: 1097 4703 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 3125 1870 Size: 1255 4545 XmnI GAAnn'nnTTC Cuts at: 3929 3929 Size: 5800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI