################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI StyI SwaI TaqI TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AciI 296 GCGG CCGC(-3/-1) AlwNI 380 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 463 ATTAAT AT^TAAT AsnI 463 ATTAAT AT^TAAT AspI 795 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 596 CCCGAG C^[CT]CG[GA]G BsaJI 701 CCCTGG C^C[ACGT][ACGT]GG BseRI 245 CTCCTC GAGGAG(10/8) BsgI 76 GTGCAG GTGCAG(16/14) BsoBI 596 CCCGAG C^[CT]CG[GA]G BspMI 48 ACCTGC ACCTGC(4/8) BssKI 702 CCTGG ^CC[ACGT]GG BstNI 702 CCTGG CC^[AT]GG BstYI 954 AGATCC [GA]^GATC[CT] CfoI 522 GCGC GCG^C DraI 223 TTTAAA TTT^AAA EcoRI 768 GAATTC G^AATTC EcoRII 702 CCTGG ^CC[AT]GG HhaI 522 GCGC GCG^C HinP1I 522 GCGC G^CGC MaeII 188 ACGT A^CGT MflI 954 AGATCC [GA]^GATC[CT] MvaI 702 CCTGG CC^[AT]GG MwoI 69 GCCTCTTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 178 CATATG CA^TATG PflFI 795 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 58 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 241 GACTC GAGTC(4/5) PshBI 463 ATTAAT AT^TAAT SacI 820 GAGCTC GAGCT^C ScrFI 702 CCTGG CC^[ACGT]GG SspI 460 AATATT AAT^ATT Tth111I 795 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 58 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 28 CCAACTCTTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 954 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 356 GTCTAC GT^[AC][GT]AC 775 GTAGAC AfaI 561 GTAC GT^AC 683 GTAC AspHI 731 GTGCTC G[AT]GC[AT]^C 820 GAGCTC BanII 593 GAGCCC G[GA]GC[CT]^C 820 GAGCTC BbvI 78 GCAGC GCAGC(8/12) 886 GCTGC BclI 9 TGATCA T^GATCA 828 TGATCA BsiHKAI 731 GTGCTC G[AT]GC[AT]^C 820 GAGCTC BsmAI 411 GAGAC GTCTC(1/5) 801 GTCTC Cfr13I 67 GGGCC G^G[ACGT]CC 808 GGGCC FbaI 9 TGATCA T^GATCA 828 TGATCA Fnu4HI 78 GCAGC GC^[ACGT]GC 886 GCTGC ItaI 78 GCAGC GC^[ACGT]GC 886 GCTGC NlaIV 535 GGAACC GG[ACGT]^[ACGT]CC 807 GGGGCC NspI 81 GCATGC [GA]CATG^[CT] 917 GCATGC PstI 50 CTGCAG CTGCA^G 980 CTGCAG RsaI 561 GTAC GT^AC 683 GTAC Sau96I 67 GGGCC G^G[ACGT]CC 808 GGGCC SfaNI 106 GATGC GCATC(5/9) 550 GCATC SphI 81 GCATGC GCATG^C 917 GCATGC TseI 78 GCAGC G^C[AT]GC 886 GCTGC Tsp45I 42 GTCAC ^GT[GC]AC 739 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AcsI AluI AlwI ApoI BfaI BmyI BsiYI BslI Bsp1286I BstF5I Cac8I DdeI DpnII FokI HaeIII HinfI HphI MaeI MaeIII MboI MboII MnlI MseI NdeII NlaIII Sau3AI SfcI TfiI Tru9I Tsp509I TspRI