(Circular) (Six-base) MAPSORT of: pFN21AB7922.seq Check: 2927 from: 1 to: 5746 pFN21AB7922 5746 bp With 182 enzymes: SgfI * March 28, 2012 12:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2362 2781 3089 1937 Size: 425 419 308 4594 Fragments arranged by size: 4594 425 419 308 Acc65I G'GTAC_C Cuts at: 3067 3067 Size: 5746 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3809 5193 5461 1632 Size: 2177 1384 268 1917 Fragments arranged by size: 2177 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 4645 5622 1203 Size: 3442 977 1327 Fragments arranged by size: 3442 1327 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5746 AlwNI CAG_nnn'CTG Cuts at: 2390 3489 5061 2390 Size: 1099 1572 3075 Fragments arranged by size: 3075 1572 1099 ApaLI G'TGCA_C Cuts at: 3803 4959 3803 Size: 1156 4590 ApoI r'AATT_y Cuts at: 1493 2313 2676 2773 3055 3281 3433 5542 1493 Size: 820 363 97 282 226 152 2109 1697 Fragments arranged by size: 2109 1697 820 363 282 226 152 97 AseI AT'TA_AT Cuts at: 160 1032 2469 4300 160 Size: 872 1437 1831 1606 Fragments arranged by size: 1831 1606 1437 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2601 3071 1870 Size: 73 658 470 4545 Fragments arranged by size: 4545 658 470 73 BamHI G'GATC_C Cuts at: 3076 3443 3076 Size: 367 5379 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3067 4522 618 Size: 325 217 627 1280 1455 1842 Fragments arranged by size: 1842 1455 1280 627 325 217 BanII G_rGCy'C Cuts at: 1391 2602 2829 3065 1391 Size: 1211 227 236 4072 Fragments arranged by size: 4072 1211 236 227 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4775 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3937 3971 1903 Size: 34 2000 34 3678 Fragments arranged by size: 3678 2000 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3638 4848 3638 Size: 1210 4536 BclI T'GATC_A Cuts at: 1548 2014 2833 1548 Size: 466 819 4461 Fragments arranged by size: 4461 819 466 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4357 136 Size: 107 122 71 631 605 2685 1525 Fragments arranged by size: 2685 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5741 5741 Size: 5746 BlpI GC'TnA_GC Cuts at: 3163 3163 Size: 5746 Bme1580I G_kGCm'C Cuts at: 1790 3807 4963 1790 Size: 2017 1156 2573 Fragments arranged by size: 2573 2017 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4435 442 Size: 416 53 83 399 24 2060 958 1753 Fragments arranged by size: 2060 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4406 1239 Size: 3167 2579 Bpu10I CC'TnA_GC Cuts at: 2766 2823 2766 Size: 57 5689 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3871 4736 5034 3871 Size: 865 298 4583 Fragments arranged by size: 4583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4409 882 Size: 617 2910 2219 Fragments arranged by size: 2910 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3537 493 Size: 711 2333 2702 Fragments arranged by size: 2702 2333 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3553 3935 4570 275 Size: 53 83 186 936 2020 382 635 1451 Fragments arranged by size: 2020 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4179 4577 4851 4998 5409 4179 Size: 398 274 147 411 4516 Fragments arranged by size: 4516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2929 2959 1178 Size: 30 165 30 1526 30 3965 Fragments arranged by size: 3965 1526 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2241 1186 Size: 1055 4691 BseYI C'CCAG_C Cuts at: 4949 5445 4949 Size: 496 5250 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2102 1163 Size: 573 366 4807 Fragments arranged by size: 4807 573 366 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3957 4985 1540 Size: 275 186 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2740 2829 3807 3892 4963 2740 Size: 89 978 85 1071 3523 Fragments arranged by size: 3523 1071 978 89 85 BsmI GAATG_Cn' Cuts at: 3257 3350 3257 Size: 93 5653 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2602 2740 2829 3065 3807 3892 4963 Size: 399 812 138 89 236 742 85 1071 Cuts at: 4963 1391 Size: 2174 Fragments arranged by size: 2174 1071 812 742 399 236 138 89 85 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2062 3101 844 Size: 1218 1039 3489 Fragments arranged by size: 3489 1218 1039 BsrBI CCG'CTC Cuts at: 1394 1823 3633 1394 Size: 429 1810 3507 Fragments arranged by size: 3507 1810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4241 4415 65 Size: 1183 2993 174 1396 Fragments arranged by size: 2993 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4390 4577 1700 Size: 110 2580 187 2869 Fragments arranged by size: 2869 2580 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5746 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5746 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5746 BstYI r'GATC_y Cuts at: 1972 2959 3076 3443 3828 3845 5297 5383 5395 Size: 987 117 367 385 17 1452 86 12 Cuts at: 5395 5741 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 987 385 367 346 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5194 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5007 Fragments arranged by size: 5007 683 56 BtsI GCAGTG_nn' Cuts at: 2064 3262 4056 4076 2064 Size: 1198 794 20 3734 Fragments arranged by size: 3734 1198 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 2230 3050 2230 Size: 820 4926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4753 809 Size: 507 3437 1802 Fragments arranged by size: 3437 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3193 4082 5711 Size: 54 1425 50 275 18 1363 889 1629 Cuts at: 5711 8 Size: 43 Fragments arranged by size: 1629 1425 1363 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3676 1353 Size: 2323 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4333 4707 4853 387 Size: 1064 138 2744 374 146 1280 Fragments arranged by size: 2744 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2827 2827 Size: 5746 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3809 4406 5193 5461 1239 Size: 393 2177 597 787 268 1524 Fragments arranged by size: 2177 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2773 3055 3433 2773 Size: 282 378 5086 Fragments arranged by size: 5086 378 282 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2364 2364 Size: 5746 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 4893 1678 Size: 3215 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2014 2031 2046 2791 Size: 32 135 32 427 15 17 15 745 Cuts at: 2791 2823 2846 2878 2927 2959 4387 4419 4461 Size: 32 23 32 49 32 1428 32 42 Cuts at: 4461 4493 1373 Size: 32 2626 Fragments arranged by size: 2626 1428 745 427 135 49 42 32 32 32 32 32 32 32 23 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 3090 3336 669 Size: 988 281 1152 246 3079 Fragments arranged by size: 3079 1152 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5746 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 3071 3071 Size: 5746 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2057 4860 5044 1305 Size: 752 2803 184 2007 Fragments arranged by size: 2803 2007 752 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3924 Fragments arranged by size: 3924 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3704 4063 4222 518 Size: 648 2538 359 159 2042 Fragments arranged by size: 2538 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3162 3839 4987 5232 1606 Size: 1556 677 1148 245 2120 Fragments arranged by size: 2120 1556 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5194 NdeI CA'TA_TG Cuts at: 387 2184 387 Size: 1797 3949 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 2090 2926 3104 4649 1132 Size: 97 861 836 178 1545 2229 Fragments arranged by size: 2229 1545 861 836 178 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5746 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 2069 1108 Size: 961 4785 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2947 2979 3815 3847 5328 5360 2947 Size: 32 836 32 1481 32 3333 Fragments arranged by size: 3333 1481 836 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5746 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PstI C_TGCA'G Cuts at: 830 2059 2989 3098 830 Size: 1229 930 109 3478 Fragments arranged by size: 3478 1229 930 109 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 SacI G_AGCT'C Cuts at: 2829 2829 Size: 5746 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SfcI C'TryA_G Cuts at: 826 1045 2055 2841 2985 3094 4229 4910 5101 Size: 219 1010 786 144 109 1135 681 191 Cuts at: 5101 826 Size: 1471 Fragments arranged by size: 1471 1135 1010 786 681 219 191 144 109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 3073 1872 Size: 1201 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3850 4751 5013 820 Size: 197 926 1907 901 262 1553 Fragments arranged by size: 1907 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 2090 2926 3104 1132 Size: 958 836 178 3774 Fragments arranged by size: 3774 958 836 178 SspI AAT'ATT Cuts at: 5 52 2467 3670 5 Size: 47 2415 1203 2081 Fragments arranged by size: 2415 2081 1203 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5746 StyI C'CwwG_G Cuts at: 513 1065 3185 513 Size: 552 2120 3074 Fragments arranged by size: 3074 2120 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3773 3790 3943 4128 4573 325 Size: 1255 2193 17 153 185 445 1498 Fragments arranged by size: 2193 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2565 3992 5704 Size: 274 80 33 51 1443 588 1427 1712 Cuts at: 5704 96 Size: 138 Fragments arranged by size: 1712 1443 1427 588 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2485 2517 2929 2961 5706 5738 2485 Size: 32 412 32 2745 32 2493 Fragments arranged by size: 2745 2493 412 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2803 1316 Size: 1487 4259 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2040 2040 Size: 5746 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5746 XmaI C'CCGG_G Cuts at: 1870 3071 1870 Size: 1201 4545 XmnI GAAnn'nnTTC Cuts at: 3875 3875 Size: 5746 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI