(Linear) (Six-base) MAPSORT of: KIEE5741.seq Check: 6871 from: 1 to: 1869 KIEE5741.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 870 1869 Size: 870 999 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 151 415 671 1329 1869 Size: 31 120 264 256 658 540 Fragments arranged by size: 658 540 264 256 120 31 AflII C'TTAA_G Cuts at: 0 504 1869 Size: 504 1365 AhdI GACnn_n'nnGTC Cuts at: 0 714 1869 Size: 714 1155 AlwNI CAG_nnn'CTG Cuts at: 0 420 1869 Size: 420 1449 ApoI r'AATT_y Cuts at: 0 1210 1310 1869 Size: 1210 100 559 Fragments arranged by size: 1210 559 100 BanII G_rGCy'C Cuts at: 0 971 1869 Size: 971 898 BbsI GAAGACnn'nnnn_ Cuts at: 0 363 569 677 919 1658 1778 1829 1869 Size: 363 206 108 242 739 120 51 40 Fragments arranged by size: 739 363 242 206 120 108 51 40 BbvCI CC'TCA_GC Cuts at: 0 26 68 1715 1869 Size: 26 42 1647 154 Fragments arranged by size: 1647 154 42 26 BclI T'GATC_A Cuts at: 0 17 1869 Size: 17 1852 BfrBI ATG'CAT Cuts at: 0 1621 1869 Size: 1621 248 BglII A'GATC_T Cuts at: 0 1860 1869 Size: 1860 9 BlpI GC'TnA_GC Cuts at: 0 424 1869 Size: 424 1445 Bme1580I G_kGCm'C Cuts at: 0 1133 1869 Size: 1133 736 BmgBI CAC'GTC Cuts at: 0 1740 1869 Size: 1740 129 BmrI ACTGGGnnnn_n' Cuts at: 0 740 1075 1869 Size: 740 335 794 Fragments arranged by size: 794 740 335 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1070 1869 Size: 1070 799 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 388 566 1869 Size: 373 15 178 1303 Fragments arranged by size: 1303 373 178 15 Bpu10I CC'TnA_GC Cuts at: 0 26 68 1715 1869 Size: 26 42 1647 154 Fragments arranged by size: 1647 154 42 26 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 479 1869 Size: 479 1390 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 595 625 1869 Size: 595 30 1244 Fragments arranged by size: 1244 595 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 56 603 615 739 1869 Size: 56 547 12 124 1130 Fragments arranged by size: 1130 547 124 56 12 BseYI C'CCAG_C Cuts at: 0 359 1119 1869 Size: 359 760 750 Fragments arranged by size: 760 750 359 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1381 1869 Size: 1381 488 BsmI GAATG_Cn' Cuts at: 0 1377 1869 Size: 1377 492 Bsp1286I G_dGCh'C Cuts at: 0 971 1133 1869 Size: 971 162 736 Fragments arranged by size: 971 736 162 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 870 1869 Size: 870 999 BsrDI GCAATG_nn' Cuts at: 0 941 1692 1869 Size: 941 751 177 Fragments arranged by size: 941 751 177 BstAPI GCAn_nnn'nTGC Cuts at: 0 420 885 1869 Size: 420 465 984 Fragments arranged by size: 984 465 420 BstYI r'GATC_y Cuts at: 0 41 1860 1869 Size: 41 1819 9 Fragments arranged by size: 1819 41 9 BtgI C'CryG_G Cuts at: 0 756 1869 Size: 756 1113 BtsI GCAGTG_nn' Cuts at: 0 854 1869 Size: 854 1015 DraI TTT'AAA Cuts at: 0 815 1869 Size: 815 1054 EaeI y'GGCC_r Cuts at: 0 462 753 772 1869 Size: 462 291 19 1097 Fragments arranged by size: 1097 462 291 19 EarI CTCTTCn'nnn_ Cuts at: 0 1742 1838 1869 Size: 1742 96 31 Fragments arranged by size: 1742 96 31 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 151 373 388 415 566 671 1329 Size: 31 120 222 15 27 151 105 658 Cuts at: 1329 1869 Size: 540 Fragments arranged by size: 658 540 222 151 120 105 31 27 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 1636 1869 Size: 1636 233 EcoO109I rG'GnC_Cy Cuts at: 0 734 1102 1869 Size: 734 368 767 Fragments arranged by size: 767 734 368 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 9 41 700 732 1837 1869 Size: 9 32 659 32 1105 32 Fragments arranged by size: 1105 659 32 32 32 9 HincII GTy'rAC Cuts at: 0 1298 1869 Size: 1298 571 HpaI GTT'AAC Cuts at: 0 1298 1869 Size: 1298 571 MfeI C'AATT_G Cuts at: 0 349 1869 Size: 349 1520 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 114 653 1010 1522 1869 Size: 114 539 357 512 347 Fragments arranged by size: 539 512 357 347 114 MscI TGG'CCA Cuts at: 0 464 755 774 1869 Size: 464 291 19 1095 Fragments arranged by size: 1095 464 291 19 MslI CAynn'nnrTG Cuts at: 0 1180 1869 Size: 1180 689 MspA1I CmG'CkG Cuts at: 0 1319 1869 Size: 1319 550 NcoI C'CATG_G Cuts at: 0 756 1869 Size: 756 1113 NsiI A_TGCA'T Cuts at: 0 1623 1869 Size: 1623 246 NspI r_CATG'y Cuts at: 0 957 1869 Size: 957 912 PasI CC'CwG_GG Cuts at: 0 1098 1869 Size: 1098 771 PfoI T'CCnGG_A Cuts at: 0 300 1602 1869 Size: 300 1302 267 Fragments arranged by size: 1302 300 267 PpuMI rG'GwC_Cy Cuts at: 0 1102 1869 Size: 1102 767 PsiI TTA'TAA Cuts at: 0 1217 1869 Size: 1217 652 PstI C_TGCA'G Cuts at: 0 116 1640 1869 Size: 116 1524 229 Fragments arranged by size: 1524 229 116 PvuII CAG'CTG Cuts at: 0 1319 1869 Size: 1319 550 SanDI GG'GwC_CC Cuts at: 0 1102 1869 Size: 1102 767 SapI GCTCTTCn'nnn_ Cuts at: 0 1742 1838 1869 Size: 1742 96 31 Fragments arranged by size: 1742 96 31 SfcI C'TryA_G Cuts at: 0 112 1636 1869 Size: 112 1524 233 Fragments arranged by size: 1524 233 112 SmlI C'TyrA_G Cuts at: 0 494 504 1869 Size: 494 10 1365 Fragments arranged by size: 1365 494 10 StyI C'CwwG_G Cuts at: 0 756 1568 1869 Size: 756 812 301 Fragments arranged by size: 812 756 301 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 863 1869 Size: 863 1006 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 464 496 1869 Size: 464 32 1373 Fragments arranged by size: 1373 464 32 XmnI GAAnn'nnTTC Cuts at: 0 926 1869 Size: 926 943 Enzymes that do cut: AarI AcuI AflII AhdI AlwNI ApoI BanII BbsI BbvCI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspMI BsrDI BstAPI BstYI BtgI BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I Hin4I HincII HpaI MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PfoI PpuMI PsiI PstI PvuII SanDI SapI SfcI SmlI StyI TaqII TstI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflIII AgeI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI BglI BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PciI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI