################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI HinP1I HindIII HpaII KasI KpnI KspI MfeI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 256 GTCTAC GT^[AC][GT]AC AciI 10 GCGG CCGC(-3/-1) AcyI 1302 GACGCC G[GA]^CG[CT]C AocI 358 CCTGAGG CC^T[ACGT]AGG AseI 1072 ATTAAT AT^TAAT AsnI 1072 ATTAAT AT^TAAT BanI 915 GGCACC G^G[CT][GA]CC BciVI 468 GTATCC GTATCC(6/5) BfaI 494 CTAG C^TAG BpmI 1327 CTGGAG CTGGAG(16/14) BsaBI 325 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1302 GACGCC G[GA]^CG[CT]C BsaI 1343 GAGACC GGTCTC(1/5) BsaJI 1224 CCTTGG C^C[ACGT][ACGT]GG BseRI 587 GAGGAG GAGGAG(10/8) BsmI 112 GAATGC GAATGC(1/-1) BstAPI 664 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 358 CCTGAGG CC^T[ACGT]AGG DraII 1244 AGGACCC [GA]G^G[ACGT]CC[CT] Eco81I 358 CCTGAGG CC^T[ACGT]AGG EcoNI 706 CCTGTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1244 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 1224 CCTTGG C^C[AT][AT]GG HgaI 1302 GACGC GACGC(5/10) Hin1I 1302 GACGCC G[GA]^CG[CT]C HpaI 637 GTTAAC GTT^AAC MaeI 494 CTAG C^TAG MaeII 1235 ACGT A^CGT MamI 325 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 1441 CAGCTG C[AC]G^C[GT]G PpuMI 1244 AGGACCC [GA]G^G[AT]CC[CT] PshBI 1072 ATTAAT AT^TAAT PvuII 1441 CAGCTG CAG^CTG ScaI 203 AGTACT AGT^ACT StyI 1224 CCTTGG C^C[AT][AT]GG Tsp45I 1399 GTGAC ^GT[GC]AC XbaI 493 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 489 AAATTC [GA]^AATT[CT] 1314 GAATTT AlwNI 812 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1250 CAGTTGCTG ApoI 489 AAATTC [GA]^AATT[CT] 1314 GAATTT AvaII 1245 GGACC G^G[AT]CC 1271 GGTCC BglII 879 AGATCT A^GATCT 1176 AGATCT Cac8I 610 GCTGGC GC[ACGT]^[ACGT]GC 1305 GCCAGC EarI 391 CTCTTC CTCTTC(1/4) 594 CTCTTC HincII 637 GTTAAC GT[CT]^[GA]AC 1167 GTTGAC HindII 637 GTTAAC GT[CT]^[GA]AC 1167 GTTGAC Ksp632I 391 CTCTTC CTCTTC(1/4) 594 CTCTTC MwoI 10 GCGGCAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 664 GCAGGACTTGC NlaIV 915 GGCACC GG[ACGT]^[ACGT]CC 1245 GGACCC PleI 46 GACTC GAGTC(4/5) 590 GAGTC SfcI 582 CTACAG C^T[GA][CT]AG 1159 CTACAG SmlI 313 CTTGAG C^T[CT][GA]AG 1348 CTTGAG TfiI 3 GAATC G^A[AT]TC 937 GATTC XcmI 415 CCAGAGTGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 600 CCAAGAGACTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AfaI AluI AlwI BalI BbsI BbvI BpuAI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MvaI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfaNI SspI Tru9I TseI Tsp509I TspRI XhoII