Restriction map for
pFN21AE1941
(Circular) (Six-base) MAPSORT of: pFN21AE1941.seq Check: 3526 from: 1 to: 6178
pFN21AE1941 6178 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3988 278
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2262 3521 1937
Size: 325 1259 4594
Fragments arranged by size:
4594 1259 325
Acc65I G'GTAC_C
Cuts at: 3499 3499
Size: 6178
AclI AA'CG_TT
Cuts at: 1563 4305 4678 1563
Size: 2742 373 3063
Fragments arranged by size:
3063 2742 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4241 5625 5893 1632
Size: 2609 1384 268 1917
Fragments arranged by size:
2609 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5981
AflIII A'CryG_T
Cuts at: 1203 5077 6054 1203
Size: 3874 977 1327
Fragments arranged by size:
3874 1327 977
AgeI A'CCGG_T
Cuts at: 5009 5009
Size: 6178
AhdI GACnn_n'nnGTC
Cuts at: 4907 4907
Size: 6178
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6178
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2692 2724 2692
Size: 32 6146
AlwNI CAG_nnn'CTG
Cuts at: 2822 3260 3921 5493 2822
Size: 438 661 1572 3507
Fragments arranged by size:
3507 1572 661 438
ApaLI G'TGCA_C
Cuts at: 4235 5391 4235
Size: 1156 5022
ApoI r'AATT_y
Cuts at: 1493 2494 3319 3487 3713 3865 5974 1493
Size: 1001 825 168 226 152 2109 1697
Fragments arranged by size:
2109 1697 1001 825 226 168 152
AseI AT'TA_AT
Cuts at: 160 1032 3078 4732 160
Size: 872 2046 1654 1606
Fragments arranged by size:
2046 1654 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
AvaI C'yCGr_G
Cuts at: 1870 1943 3503 1870
Size: 73 1560 4545
Fragments arranged by size:
4545 1560 73
BamHI G'GATC_C
Cuts at: 3508 3875 3508
Size: 367 5811
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2920 3499 4954 618
Size: 325 217 627 1133 579 1455 1842
Fragments arranged by size:
1842 1455 1133 627 579 325 217
BanII G_rGCy'C
Cuts at: 1391 3497 1391
Size: 2106 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2138 3235 3379 928
Size: 971 239 1097 144 3727
Fragments arranged by size:
3727 1097 971 239 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4369 4403 1903
Size: 34 2432 34 3678
Fragments arranged by size:
3678 2432 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2484 4070 5280 2484
Size: 1586 1210 3382
Fragments arranged by size:
3382 1586 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4789 136
Size: 107 122 71 631 605 3117 1525
Fragments arranged by size:
3117 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2884 3181 6173 2884
Size: 297 2992 2889
Fragments arranged by size:
2992 2889 297
BlpI GC'TnA_GC
Cuts at: 3595 3595
Size: 6178
Bme1580I G_kGCm'C
Cuts at: 1790 4239 5395 1790
Size: 2449 1156 2573
Fragments arranged by size:
2573 2449 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6178
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3248 3909 4867
Size: 416 53 83 399 24 1831 661 958
Cuts at: 4867 442
Size: 1753
Fragments arranged by size:
1831 1753 958 661 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3353 4838 1239
Size: 2114 1485 2579
Fragments arranged by size:
2579 2114 1485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 3374 4303 5168 5466 2339
Size: 1035 929 865 298 3051
Fragments arranged by size:
3051 1035 929 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3342 4841 882
Size: 617 1843 1499 2219
Fragments arranged by size:
2219 1843 1499 617
BsaAI yAC'GTr
Cuts at: 493 1204 3969 493
Size: 711 2765 2702
Fragments arranged by size:
2765 2702 711
BsaBI GATnn'nnATC
Cuts at: 2334 6172 2334
Size: 3838 2340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3308 3985 4367 5002
Size: 53 83 186 936 1775 677 382 635
Cuts at: 5002 275
Size: 1451
Fragments arranged by size:
1775 1451 936 677 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4611 5009 5283 5430 5841 4611
Size: 398 274 147 411 4948
Fragments arranged by size:
4948 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5953
Fragments arranged by size:
5953 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2607 1186
Size: 1421 4757
BseYI C'CCAG_C
Cuts at: 5381 5877 5381
Size: 496 5682
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5605
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3628 4389 5417 1540
Size: 275 186 1627 761 1028 2301
Fragments arranged by size:
2301 1627 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4239 4324 5395 4239
Size: 85 1071 5022
Fragments arranged by size:
5022 1071 85
BsmI GAATG_Cn'
Cuts at: 2123 3689 3782 2123
Size: 1566 93 4519
Fragments arranged by size:
4519 1566 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3497 4239 4324 5395 1391
Size: 399 1707 742 85 1071 2174
Fragments arranged by size:
2174 1707 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5841 5841
Size: 6178
BspHI T'CATG_A
Cuts at: 4067 5797 4067
Size: 1730 4448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3533 844
Size: 2689 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4065 1394
Size: 429 2242 3507
Fragments arranged by size:
3507 2242 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4673 4847 65
Size: 1183 3425 174 1396
Fragments arranged by size:
3425 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4822 5009 1700
Size: 110 3012 187 2869
Fragments arranged by size:
3012 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6178
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6178
BssSI C'ACGA_G
Cuts at: 4238 5250 4238
Size: 1012 5166
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2675 794
Size: 1881 4297
BstBI TT'CG_AA
Cuts at: 4982 4982
Size: 6178
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6178
BstYI r'GATC_y
Cuts at: 1972 2416 2707 2884 3181 3370 3508 3875 4260
Size: 444 291 177 297 189 138 367 385
Cuts at: 4260 4277 5729 5815 5827 6173 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 444 385 367 346 297 291
189 177 138 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2364 2364
Size: 6178
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5626
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5439
Fragments arranged by size:
5439 683 56
BtsI GCAGTG_nn'
Cuts at: 3694 4488 4508 3694
Size: 794 20 5364
Fragments arranged by size:
5364 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2466 2501 3101 3136 529
Size: 35 1902 35 600 35 3571
Fragments arranged by size:
3571 1902 600 35 35 35
DraI TTT'AAA
Cuts at: 3482 3482
Size: 6178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5185 809
Size: 507 3869 1802
Fragments arranged by size:
3869 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2487 2520
Size: 54 1425 50 275 18 213 444 33
Cuts at: 2520 3625 4514 6143 8
Size: 1105 889 1629 43
Fragments arranged by size:
1629 1425 1105 889 444 275 213 54
50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 3625 1537
Size: 275 1813 4090
Fragments arranged by size:
4090 1813 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2402 2605 4108 1353
Size: 1049 203 1503 3423
Fragments arranged by size:
3423 1503 1049 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4765 5139 5285 387
Size: 1064 138 3176 374 146 1280
Fragments arranged by size:
3176 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3353 4241 4838 5625 5893 1239
Size: 393 1721 888 597 787 268 1524
Fragments arranged by size:
1721 1524 888 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2715 2715
Size: 6178
EcoO109I rG'GnC_Cy
Cuts at: 3250 3250
Size: 6178
EcoRI G'AATT_C
Cuts at: 3487 3865 3487
Size: 378 5800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3368 3368
Size: 6178
FspI TGC'GCA
Cuts at: 4684 4684
Size: 6178
HaeII r_GCGC'y
Cuts at: 1678 5325 1678
Size: 3647 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3166 3198 4819 4851 4893
Size: 32 135 32 1594 32 1621 32 42
Cuts at: 4893 4925 1373
Size: 32 2626
Fragments arranged by size:
2626 1621 1594 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2644 3174 3522 3768 669
Size: 988 281 706 530 348 246 3079
Fragments arranged by size:
3079 988 706 530 348 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6178
HpaI GTT'AAC
Cuts at: 2644 3768 2644
Size: 1124 5054
KpnI G_GTAC'C
Cuts at: 3503 3503
Size: 6178
MfeI C'AATT_G
Cuts at: 3777 3777
Size: 6178
MluI A'CGCG_T
Cuts at: 6054 6054
Size: 6178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5292 5476 1305
Size: 3987 184 2007
Fragments arranged by size:
3987 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 2489 2522 10
Size: 54 1425 343 213 444 33 3666
Fragments arranged by size:
3666 1425 444 343 213 54 33
MslI CAynn'nnrTG
Cuts at: 518 1166 4136 4495 4654 518
Size: 648 2970 359 159 2042
Fragments arranged by size:
2970 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3448 3594 4271 5419 5664 1606
Size: 1842 146 677 1148 245 2120
Fragments arranged by size:
2120 1842 1148 677 245 146
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5626
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6178
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6178
NotI GC'GGCC_GC
Cuts at: 3625 3625
Size: 6178
NspI r_CATG'y
Cuts at: 1132 1229 3536 5081 1132
Size: 97 2307 1545 2229
Fragments arranged by size:
2307 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6178
PciI A'CATG_T
Cuts at: 5077 5077
Size: 6178
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6178
PfoI T'CCnGG_A
Cuts at: 2244 2244
Size: 6178
PmeI GTTT'AAAC
Cuts at: 3482 3482
Size: 6178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2376 2408 4247 4279 5760 5792 2376
Size: 32 1839 32 1481 32 2762
Fragments arranged by size:
2762 1839 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3250 3250
Size: 6178
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6178
PsiI TTA'TAA
Cuts at: 2856 3748 2856
Size: 892 5286
PstI C_TGCA'G
Cuts at: 830 3530 830
Size: 2700 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6178
PvuII CAG'CTG
Cuts at: 3448 3448
Size: 6178
SalI G'TCGA_C
Cuts at: 1936 3520 1936
Size: 1584 4594
SbfI CC_TGCA'GG
Cuts at: 3530 3530
Size: 6178
ScaI AGT'ACT
Cuts at: 2210 4426 6138 2210
Size: 2216 1712 2250
Fragments arranged by size:
2250 2216 1712
SfcI C'TryA_G
Cuts at: 826 1045 2587 3164 3526 4661 5342 5533 826
Size: 219 1542 577 362 1135 681 191 1471
Fragments arranged by size:
1542 1471 1135 681 577 362 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6178
SmaI CCC'GGG
Cuts at: 1872 3505 1872
Size: 1633 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2318 3353 4282 5183 5445 820
Size: 197 926 375 1035 929 901 262 1553
Fragments arranged by size:
1553 1035 929 926 901 375 262 197
SnaBI TAC'GTA
Cuts at: 493 3969 493
Size: 3476 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6178
SphI G_CATG'C
Cuts at: 1132 3536 1132
Size: 2404 3774
SspI AAT'ATT
Cuts at: 5 52 2517 2528 2693 3136 3454 4102 5
Size: 47 2465 11 165 443 318 648 2081
Fragments arranged by size:
2465 2081 648 443 318 165 47 11
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6178
StyI C'CwwG_G
Cuts at: 513 1065 3229 3617 513
Size: 552 2164 388 3074
Fragments arranged by size:
3074 2164 552 388
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2101 4205 4222 4375 4560 5005 325
Size: 1255 521 2104 17 153 185 445 1498
Fragments arranged by size:
2104 1498 1255 521 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2193 2208 2912
Size: 274 80 33 51 1443 216 15 704
Cuts at: 2912 4424 6136 96
Size: 1512 1712 138
Fragments arranged by size:
1712 1512 1443 704 274 216 138 80
51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6138 6170 6138
Size: 32 6146
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6178
XbaI T'CTAG_A
Cuts at: 2498 3514 2498
Size: 1016 5162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2427 2612 2427
Size: 185 5993
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6178
XmaI C'CCGG_G
Cuts at: 1870 3503 1870
Size: 1633 4545
XmnI GAAnn'nnTTC
Cuts at: 4307 4307
Size: 6178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3986 276
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI