################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AfaI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI Fnu4HI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeII MamI MfeI MluI MslI MspA1I MunI MvnI MwoI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TseI Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 470 TCCGGA T^CCGGA AciI 136 CCGC CCGC(-3/-1) Alw44I 436 GTGCAC G^TGCAC AlwNI 374 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 460 CCTGAGG CC^T[ACGT]AGG ApaLI 436 GTGCAC G^TGCAC AspHI 436 GTGCAC G[AT]GC[AT]^C BalI 56 TGGCCA TGG^CCA BamHI 116 GGATCC G^GATCC BbsI 259 GTCTTC GAAGAC(2/6) BfaI 121 CTAG C^TAG BmyI 436 GTGCAC G[AGT]GC[ACT]^C BpmI 380 CTGGAG CTGGAG(16/14) BpuAI 259 GTCTTC GAAGAC(2/6) BsaJI 105 CCAAGG C^C[ACGT][ACGT]GG BseAI 470 TCCGGA T^CCGGA BsiHKAI 436 GTGCAC G[AT]GC[AT]^C BsmAI 527 GAGAC GTCTC(1/5) BsmFI 185 GGGAC GGGAC(10/14) Bsp1286I 436 GTGCAC G[AGT]GC[ACT]^C BspEI 470 TCCGGA T^CCGGA BsrFI 222 GCCGGC [GA]^CCGG[CT] Bsu36I 460 CCTGAGG CC^T[ACGT]AGG Cfr10I 222 GCCGGC [GA]^CCGG[CT] DraI 492 TTTAAA TTT^AAA DraII 27 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 342 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 19 CTCTTC CTCTTC(1/4) Eco81I 460 CCTGAGG CC^T[ACGT]AGG EcoNI 228 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 27 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 105 CCAAGG C^C[AT][AT]GG FauI 135 CCCGC CCCGC(4/6) FseI 221 GGCCGGCC GGCCGG^CC HphI 252 GGTGA GGTGA(8/7) Ksp632I 19 CTCTTC CTCTTC(1/4) MaeI 121 CTAG C^TAG MaeIII 142 GTTAC ^GT[ACGT]AC MluNI 56 TGGCCA TGG^CCA MroI 470 TCCGGA T^CCGGA MscI 56 TGGCCA TGG^CCA MseI 493 TTAA T^TAA NaeI 222 GCCGGC GCC^GGC NgoMIV 222 GCCGGC G^CCGGC NspI 138 GCATGT [GA]CATG^[CT] PflMI 373 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 211 GAGTC GAGTC(4/5) StyI 105 CCAAGG C^C[AT][AT]GG TaqI 176 TCGA T^CGA TfiI 73 GAATC G^A[AT]TC Tru9I 493 TTAA T^TAA TthHB8I 176 TCGA T^CGA Van91I 373 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BsaWI 284 ACCGGA [AT]^CCGG[AT] 470 TCCGGA BseRI 463 GAGGAG GAGGAG(10/8) 535 GAGGAG BssKI 373 CCAGG ^CC[ACGT]GG 447 CCTGG BstNI 373 CCAGG CC^[AT]GG 447 CCTGG Cac8I 218 GCTGGC GC[ACGT]^[ACGT]GC 222 GCCGGC Cfr13I 28 GGGCC G^G[ACGT]CC 225 GGCCC EaeI 56 TGGCCA [CT]^GGCC[GA] 220 TGGCCG EcoRI 172 GAATTC G^AATTC 478 GAATTC EcoRII 373 CCAGG ^CC[AT]GG 447 CCTGG HinfI 73 GAATC G^A[ACGT]TC 211 GAGTC MvaI 373 CCAGG CC^[AT]GG 447 CCTGG NlaIV 27 GGGGCC GG[ACGT]^[ACGT]CC 116 GGATCC Sau96I 28 GGGCC G^G[ACGT]CC 225 GGCCC ScrFI 373 CCAGG CC^[ACGT]GG 447 CCTGG SfaNI 199 GATGC GCATC(5/9) 238 GATGC TspRI 87 CACTG CA[GC]TG[ACGT][ACGT]^ 346 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AcsI AluI AlwI ApoI BsiYI BslI BstF5I BstYI DdeI DpnII FokI HaeIII HapII HpaII MboI MboII MflI MnlI MspI NdeII NlaIII Sau3AI Tsp509I XhoII