(Linear) (Six-base) MAPSORT of: KIEE3047.seq  Check: 1468  from: 1  to: 555



KIEE3047.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     555 
   Size:        448     107
 
AlwNI CAG_nnn'CTG

Cuts at:      0     379     555 
   Size:        379     176
 
ApaLI G'TGCA_C

Cuts at:      0     436     555 
   Size:        436     119
 
ApoI r'AATT_y

Cuts at:      0     124     172     478     555 
   Size:        124      48     306      77

  Fragments arranged by size: 

                 306     124      77      48
 
BamHI G'GATC_C

Cuts at:      0     116     555 
   Size:        116     439
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     252     555 
   Size:        252     303
 
Bme1580I G_kGCm'C

Cuts at:      0     440     555 
   Size:        440     115
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     401     555 
   Size:        401     154
 
BsaWI w'CCGG_w

Cuts at:      0     284     470     555 
   Size:        284     186      85

  Fragments arranged by size: 

                 284     186      85
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     289     319     526     555 
   Size:        289      30     207      29

  Fragments arranged by size: 

                 289     207      30      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     478     550     555 
   Size:        478      72       5

  Fragments arranged by size: 

                 478      72       5
 
BsiHKAI G_wGCw'C

Cuts at:      0     440     555 
   Size:        440     115
 
Bsp1286I G_dGCh'C

Cuts at:      0     440     555 
   Size:        440     115
 
BspEI T'CCGG_A

Cuts at:      0     470     555 
   Size:        470      85
 
BsrFI r'CCGG_y

Cuts at:      0     222     555 
   Size:        222     333
 
BstYI r'GATC_y

Cuts at:      0      14     116     467     555 
   Size:         14     102     351      88

  Fragments arranged by size: 

                 351     102      88      14
 
Bsu36I CC'TnA_GG

Cuts at:      0     461     555 
   Size:        461      94
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     212     555 
   Size:        212     343
 
DraI TTT'AAA

Cuts at:      0     494     555 
   Size:        494      61
 
DraIII CAC_nnn'GTG

Cuts at:      0     347     555 
   Size:        347     208
 
EaeI y'GGCC_r

Cuts at:      0      56     220     555 
   Size:         56     164     335

  Fragments arranged by size: 

                 335     164      56
 
EarI CTCTTCn'nnn_

Cuts at:      0      25     555 
   Size:         25     530
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     401     448     555 
   Size:        401      47     107

  Fragments arranged by size: 

                 401     107      47
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     232     555 
   Size:        232     323
 
EcoO109I rG'GnC_Cy

Cuts at:      0      28     555 
   Size:         28     527
 
EcoRI G'AATT_C

Cuts at:      0     172     478     555 
   Size:        172     306      77

  Fragments arranged by size: 

                 306     172      77
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      36     419     555 
   Size:         36     383     136

  Fragments arranged by size: 

                 383     136      36
 
FseI GG_CCGG'CC

Cuts at:      0     226     555 
   Size:        226     329
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     101     133     239     271     526     555 
   Size:        101      32     106      32     255      29

  Fragments arranged by size: 

                 255     106     101      32      32      29
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     345     555 
   Size:        345     210
 
MscI TGG'CCA

Cuts at:      0      58     555 
   Size:         58     497
 
NaeI GCC'GGC

Cuts at:      0     224     555 
   Size:        224     331
 
NgoMIV G'CCGG_C

Cuts at:      0     222     555 
   Size:        222     333
 
NspI r_CATG'y

Cuts at:      0     142     555 
   Size:        142     413
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     379     555 
   Size:        379     176
 
StyI C'CwwG_G

Cuts at:      0     105     555 
   Size:        105     450
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     331     363     555 
   Size:        331      32     192

  Fragments arranged by size: 

                 331     192      32
 

 Enzymes that do cut:

     AcuI    AlwNI    ApaLI     ApoI    BamHI     BbsI Bme1580I     BpmI
    BsaWI    BsaXI    BseRI  BsiHKAI Bsp1286I    BspEI    BsrFI    BstYI
   Bsu36I    BtgZI     DraI   DraIII     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FseI    Hin4I     MmeI     MscI     NaeI
   NgoMIV     NspI    PflMI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I     BtgI     BtsI     ClaI    CspCI     DrdI
     EagI     EciI  EcoICRI    EcoRV     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI   MspA1I
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI