(Circular) (Six-base) MAPSORT of: pFN21AE3342.seq Check: 4203 from: 1 to: 7666 pFN21AE3342 7666 bp With 182 enzymes: SgfI * March 28, 2012 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3863 3863 Size: 7666 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3467 5476 278 Size: 53 83 186 936 1931 2009 2468 Fragments arranged by size: 2468 2009 1931 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3771 5009 1937 Size: 1834 1238 4594 Fragments arranged by size: 4594 1834 1238 Acc65I G'GTAC_C Cuts at: 3301 4191 4987 3301 Size: 890 796 5980 Fragments arranged by size: 5980 890 796 AclI AA'CG_TT Cuts at: 1563 5793 6166 1563 Size: 4230 373 3063 Fragments arranged by size: 4230 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 3479 3515 3971 5729 7113 7381 1632 Size: 482 1365 36 456 1758 1384 268 1917 Fragments arranged by size: 1917 1758 1384 1365 482 456 268 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7469 AflIII A'CryG_T Cuts at: 1203 2259 6565 7542 1203 Size: 1056 4306 977 1327 Fragments arranged by size: 4306 1327 1056 977 AgeI A'CCGG_T Cuts at: 6497 6497 Size: 7666 AhdI GACnn_n'nnGTC Cuts at: 2496 6395 2496 Size: 3899 3767 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7666 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2579 3703 1184 Size: 1395 1124 5147 Fragments arranged by size: 5147 1395 1124 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2999 3031 3224 3256 2999 Size: 32 193 32 7409 Fragments arranged by size: 7409 193 32 32 AlwNI CAG_nnn'CTG Cuts at: 4796 5409 6981 4796 Size: 613 1572 5481 Fragments arranged by size: 5481 1572 613 ApaI G_GGCC'C Cuts at: 3261 3555 3261 Size: 294 7372 ApaLI G'TGCA_C Cuts at: 2955 5723 6879 2955 Size: 2768 1156 3742 Fragments arranged by size: 3742 2768 1156 ApoI r'AATT_y Cuts at: 1493 3818 4975 5201 5353 7462 1493 Size: 2325 1157 226 152 2109 1697 Fragments arranged by size: 2325 2109 1697 1157 226 152 AseI AT'TA_AT Cuts at: 160 1032 6220 160 Size: 872 5188 1606 Fragments arranged by size: 5188 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7666 AvaI C'yCGr_G Cuts at: 1870 1943 2203 2288 2307 2640 2949 3153 3375 Size: 73 260 85 19 333 309 204 222 Cuts at: 3375 4180 4668 4933 4991 1870 Size: 805 488 265 58 4545 Fragments arranged by size: 4545 805 488 333 309 265 260 222 204 85 73 58 19 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3753 3786 3753 Size: 33 7633 BamHI G'GATC_C Cuts at: 4996 5363 4996 Size: 367 7299 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 2549 2825 3301 3379 Size: 325 217 627 462 300 276 476 78 Cuts at: 3379 3428 4100 4191 4987 6442 618 Size: 49 672 91 796 1455 1842 Fragments arranged by size: 1842 1455 796 672 627 476 462 325 300 276 217 91 78 49 BanII G_rGCy'C Cuts at: 1391 2013 3171 3261 3555 4334 4985 1391 Size: 622 1158 90 294 779 651 4072 Fragments arranged by size: 4072 1158 779 651 622 294 90 BbeI G_GCGC'C Cuts at: 2253 3432 4104 2253 Size: 1179 672 5815 Fragments arranged by size: 5815 1179 672 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2335 3467 3801 928 Size: 971 436 1132 334 4793 Fragments arranged by size: 4793 1132 971 436 334 BbvCI CC'TCA_GC Cuts at: 2790 4836 2790 Size: 2046 5620 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3622 3656 3708 3742 5857 5891 1903 Size: 34 1685 34 52 34 2115 34 3678 Fragments arranged by size: 3678 2115 1685 52 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3250 5558 6768 3250 Size: 2308 1210 4148 Fragments arranged by size: 4148 2308 1210 BclI T'GATC_A Cuts at: 1548 4491 1548 Size: 2943 4723 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2558 3009 4044 Size: 107 122 71 631 605 886 451 1035 Cuts at: 4044 4373 6277 136 Size: 329 1904 1525 Fragments arranged by size: 1904 1525 1035 886 631 605 451 329 122 107 71 BglII A'GATC_T Cuts at: 4827 7661 4827 Size: 2834 4832 BlpI GC'TnA_GC Cuts at: 2510 5083 2510 Size: 2573 5093 Bme1580I G_kGCm'C Cuts at: 1790 2203 2658 2821 2828 2938 2959 3040 3261 Size: 413 455 163 7 110 21 81 221 Cuts at: 3261 3382 3555 3635 5727 6883 1790 Size: 121 173 80 2092 1156 2573 Fragments arranged by size: 2573 2092 1156 455 413 221 173 163 121 110 81 80 21 7 BmgBI CAC'GTC Cuts at: 1318 2191 4489 1318 Size: 873 2298 4495 Fragments arranged by size: 4495 2298 873 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3378 4477 5397 Size: 416 53 83 399 24 1961 1099 920 Cuts at: 5397 6355 442 Size: 958 1753 Fragments arranged by size: 1961 1753 1099 958 920 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7666 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3083 4324 3083 Size: 1241 6425 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3065 4061 4319 4435 6326 1239 Size: 1826 996 258 116 1891 2579 Fragments arranged by size: 2579 1891 1826 996 258 116 Bpu10I CC'TnA_GC Cuts at: 2790 3247 4836 2790 Size: 457 1589 5620 Fragments arranged by size: 5620 1589 457 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3373 3890 5791 6656 6954 3373 Size: 517 1901 865 298 4085 Fragments arranged by size: 4085 1901 865 517 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2051 2324 3123 4215 6329 882 Size: 617 552 273 799 1092 2114 2219 Fragments arranged by size: 2219 2114 1092 799 617 552 273 BsaAI yAC'GTr Cuts at: 493 1204 2260 5457 493 Size: 711 1056 3197 2702 Fragments arranged by size: 3197 2702 1056 711 BsaBI GATnn'nnATC Cuts at: 4352 7660 4352 Size: 3308 4358 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2250 3429 3464 4101 Size: 53 83 186 936 717 1179 35 637 Cuts at: 4101 5473 5855 6490 275 Size: 1372 382 635 1451 Fragments arranged by size: 1451 1372 1179 936 717 637 635 382 186 83 53 35 BsaWI w'CCGG_w Cuts at: 4386 4584 4638 6099 6497 6771 6918 7329 4386 Size: 198 54 1461 398 274 147 411 4723 Fragments arranged by size: 4723 1461 411 398 274 198 147 54 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2956 2986 2999 3029 4316 Size: 30 165 30 1553 30 13 30 1287 Cuts at: 4316 4346 4430 4448 4460 4478 4680 4710 1178 Size: 30 84 18 12 18 202 30 4134 Fragments arranged by size: 4134 1553 1287 202 165 84 30 30 30 30 30 30 18 18 13 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3004 4316 1186 Size: 1818 1312 4536 Fragments arranged by size: 4536 1818 1312 BseYI C'CCAG_C Cuts at: 2028 2312 2598 2702 2708 2869 3344 3440 6869 Size: 284 286 104 6 161 475 96 3429 Cuts at: 6869 7365 2028 Size: 496 2329 Fragments arranged by size: 3429 2329 496 475 286 284 161 104 96 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2213 2558 2947 3005 3539 3891 1163 Size: 573 477 345 389 58 534 352 4938 Fragments arranged by size: 4938 573 534 477 389 352 345 58 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5116 5877 6905 1540 Size: 275 186 3115 761 1028 2301 Fragments arranged by size: 3115 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2013 2773 2959 4023 4334 5727 5812 6883 2013 Size: 760 186 1064 311 1393 85 1071 2796 Fragments arranged by size: 2796 1393 1071 1064 760 311 186 85 BsmI GAATG_Cn' Cuts at: 4594 5177 5270 4594 Size: 583 93 6990 Fragments arranged by size: 6990 583 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2203 2658 2773 2821 2828 2938 Size: 399 223 190 455 115 48 7 110 Cuts at: 2938 2959 3040 3171 3261 3382 3555 3635 4023 Size: 21 81 131 90 121 173 80 388 Cuts at: 4023 4334 4985 5727 5812 6883 1391 Size: 311 651 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 651 455 399 388 311 223 190 173 131 121 115 110 90 85 81 80 48 21 7 BspEI T'CCGG_A Cuts at: 4386 4638 7329 4386 Size: 252 2691 4723 Fragments arranged by size: 4723 2691 252 BspHI T'CATG_A Cuts at: 4071 5555 7285 4071 Size: 1484 1730 4452 Fragments arranged by size: 4452 1730 1484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3511 3863 4851 5021 844 Size: 2667 352 988 170 3489 Fragments arranged by size: 3489 2667 988 352 170 BsrBI CCG'CTC Cuts at: 1394 1823 2142 2509 3970 5553 1394 Size: 429 319 367 1461 1583 3507 Fragments arranged by size: 3507 1583 1461 429 367 319 BsrDI GCAATG_nn' Cuts at: 65 1248 3712 4377 6161 6335 65 Size: 1183 2464 665 1784 174 1396 Fragments arranged by size: 2464 1784 1396 1183 665 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2663 2749 3542 4515 4951 6310 6497 Size: 110 853 86 793 973 436 1359 187 Cuts at: 6497 1700 Size: 2869 Fragments arranged by size: 2869 1359 973 853 793 436 187 110 86 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7666 BssHII G'CGCG_C Cuts at: 1925 3530 1925 Size: 1605 6061 BssSI C'ACGA_G Cuts at: 4154 4327 5726 6738 4154 Size: 173 1399 1012 5082 Fragments arranged by size: 5082 1399 1012 173 BstAPI GCAn_nnn'nTGC Cuts at: 794 2167 794 Size: 1373 6293 BstBI TT'CG_AA Cuts at: 6470 6470 Size: 7666 BstEII G'GTnAC_C Cuts at: 1184 4051 1184 Size: 2867 4799 BstXI CCAn_nnnn'nTGG Cuts at: 3514 4765 3514 Size: 1251 6415 BstYI r'GATC_y Cuts at: 1972 4827 4996 5363 5748 5765 7217 7303 7315 Size: 2855 169 367 385 17 1452 86 12 Cuts at: 7315 7661 1972 Size: 346 1977 Fragments arranged by size: 2855 1977 1452 385 367 346 169 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2112 3692 2112 Size: 1580 6086 BtgI C'CryG_G Cuts at: 513 1065 2004 2240 2373 2430 2658 2984 3252 Size: 552 939 236 133 57 228 326 268 Cuts at: 3252 513 Size: 4927 Fragments arranged by size: 4927 939 552 326 268 236 228 133 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2373 4159 487 Size: 683 56 1147 1786 3994 Fragments arranged by size: 3994 1786 1147 683 56 BtsI GCAGTG_nn' Cuts at: 2132 3222 3318 5182 5976 5996 2132 Size: 1090 96 1864 794 20 3802 Fragments arranged by size: 3802 1864 1090 794 96 20 ClaI AT'CG_AT Cuts at: 1554 3834 1554 Size: 2280 5386 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7631 DraI TTT'AAA Cuts at: 4970 4970 Size: 7666 DraIII CAC_nnn'GTG Cuts at: 2167 2805 3386 2167 Size: 638 581 6447 Fragments arranged by size: 6447 638 581 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3407 6673 809 Size: 507 2091 3266 1802 Fragments arranged by size: 3266 2091 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2370 2427 Size: 54 1425 50 275 18 210 330 57 Cuts at: 2427 2661 2731 3055 3559 4890 5113 6002 7631 Size: 234 70 324 504 1331 223 889 1629 Cuts at: 7631 8 Size: 43 Fragments arranged by size: 1629 1425 1331 889 504 330 324 275 234 223 210 70 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5113 1537 Size: 275 3301 4090 Fragments arranged by size: 4090 3301 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2049 2585 3852 4315 5596 1353 Size: 696 536 1267 463 1281 3423 Fragments arranged by size: 3423 1281 1267 696 536 463 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4025 4390 6253 6627 6773 387 Size: 1064 138 2436 365 1863 374 146 1280 Fragments arranged by size: 2436 1863 1280 1064 374 365 146 138 EcoICRI GAG'CTC Cuts at: 2011 4332 2011 Size: 2321 5345 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 3065 3479 3515 3971 4061 4319 Size: 393 482 951 414 36 456 90 258 Cuts at: 4319 4435 5729 6326 7113 7381 1239 Size: 116 1294 597 787 268 1524 Fragments arranged by size: 1524 1294 951 787 597 482 456 414 393 268 258 116 90 36 EcoNI CCTnn'n_nnAGG Cuts at: 2150 3518 2150 Size: 1368 6298 EcoO109I rG'GnC_Cy Cuts at: 2236 2853 3064 3157 3257 3437 3552 3910 4683 Size: 617 211 93 100 180 115 358 773 Cuts at: 4683 4840 2236 Size: 157 5062 Fragments arranged by size: 5062 773 617 358 211 180 157 115 100 93 EcoRI G'AATT_C Cuts at: 4975 5353 4975 Size: 378 7288 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2350 2539 4903 2350 Size: 189 2364 5113 Fragments arranged by size: 5113 2364 189 FspI TGC'GCA Cuts at: 2171 6172 2171 Size: 4001 3665 FspAI rTGC'GCAy Cuts at: 2171 2171 Size: 7666 HaeII r_GCGC'y Cuts at: 1678 2253 2320 2455 3432 4104 4623 6813 1678 Size: 575 67 135 977 672 519 2190 2531 Fragments arranged by size: 2531 2190 977 672 575 519 135 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2095 2127 2282 2314 2482 Size: 32 135 32 523 32 155 32 168 Cuts at: 2482 2514 2623 2655 2889 2921 3732 3764 4430 Size: 32 109 32 234 32 811 32 666 Cuts at: 4430 4462 6307 6339 6381 6413 1373 Size: 32 1845 32 42 32 2626 Fragments arranged by size: 2626 1845 811 666 523 234 168 155 135 109 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4249 5010 5256 669 Size: 988 281 2311 761 246 3079 Fragments arranged by size: 3079 2311 988 761 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7666 HpaI GTT'AAC Cuts at: 5256 5256 Size: 7666 KasI G'GCGC_C Cuts at: 2249 3428 4100 2249 Size: 1179 672 5815 Fragments arranged by size: 5815 1179 672 KpnI G_GTAC'C Cuts at: 3305 4195 4991 3305 Size: 890 796 5980 Fragments arranged by size: 5980 890 796 MfeI C'AATT_G Cuts at: 5265 5265 Size: 7666 MluI A'CGCG_T Cuts at: 7542 7542 Size: 7666 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3342 6780 6964 1305 Size: 2037 3438 184 2007 Fragments arranged by size: 3438 2037 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2733 4892 10 Size: 54 1425 343 901 2159 2784 Fragments arranged by size: 2784 2159 1425 901 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 3326 4763 5624 5983 6142 518 Size: 648 1248 912 1437 861 359 159 2042 Fragments arranged by size: 2042 1437 1248 912 861 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2432 2602 2766 2789 2986 3084 3616 3976 Size: 826 170 164 23 197 98 532 360 Cuts at: 3976 4708 4796 5082 5759 6907 7152 1606 Size: 732 88 286 677 1148 245 2120 Fragments arranged by size: 2120 1148 826 732 677 532 360 286 245 197 170 164 98 88 23 NaeI GCC'GGC Cuts at: 3544 3544 Size: 7666 NarI GG'CG_CC Cuts at: 2250 3429 4101 2250 Size: 1179 672 5815 Fragments arranged by size: 5815 1179 672 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6175 Fragments arranged by size: 6175 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7666 NgoMIV G'CCGG_C Cuts at: 3542 3542 Size: 7666 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7666 NotI GC'GGCC_GC Cuts at: 5113 5113 Size: 7666 NspI r_CATG'y Cuts at: 1132 1229 4066 4165 4189 5024 6569 1132 Size: 97 2837 99 24 835 1545 2229 Fragments arranged by size: 2837 2229 1545 835 99 97 24 PasI CC'CwG_GG Cuts at: 1348 2456 2821 3171 3914 4336 1348 Size: 1108 365 350 743 422 4678 Fragments arranged by size: 4678 1108 743 422 365 350 PciI A'CATG_T Cuts at: 6565 6565 Size: 7666 PflMI CCAn_nnn'nTGG Cuts at: 1108 4156 4359 1108 Size: 3048 203 4415 Fragments arranged by size: 4415 3048 203 PfoI T'CCnGG_A Cuts at: 2538 2625 4296 4698 2538 Size: 87 1671 402 5506 Fragments arranged by size: 5506 1671 402 87 PmeI GTTT'AAAC Cuts at: 4970 4970 Size: 7666 PmlI CAC'GTG Cuts at: 2260 2260 Size: 7666 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2999 3031 5735 5767 7248 7280 2999 Size: 32 2704 32 1481 32 3385 Fragments arranged by size: 3385 2704 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2236 2853 3064 3157 3437 4840 2236 Size: 617 211 93 280 1403 5062 Fragments arranged by size: 5062 1403 617 280 211 93 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7666 PsiI TTA'TAA Cuts at: 5236 5236 Size: 7666 PspOMI G'GGCC_C Cuts at: 3257 3551 3257 Size: 294 7372 PspXI vC'TCGA_Gb Cuts at: 2288 2288 Size: 7666 PstI C_TGCA'G Cuts at: 830 2765 3313 5018 830 Size: 1935 548 1705 3478 Fragments arranged by size: 3478 1935 1705 548 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7666 PvuII CAG'CTG Cuts at: 2602 2766 2789 3616 2602 Size: 164 23 827 6652 Fragments arranged by size: 6652 827 164 23 SacI G_AGCT'C Cuts at: 2013 4334 2013 Size: 2321 5345 SacII CC_GC'GG Cuts at: 2433 2987 2433 Size: 554 7112 SalI G'TCGA_C Cuts at: 1936 5008 1936 Size: 3072 4594 SanDI GG'GwC_CC Cuts at: 2236 3437 2236 Size: 1201 6465 SbfI CC_TGCA'GG Cuts at: 5018 5018 Size: 7666 ScaI AGT'ACT Cuts at: 5914 7626 5914 Size: 1712 5954 SexAI A'CCwGG_T Cuts at: 3159 3513 3159 Size: 354 7312 SfcI C'TryA_G Cuts at: 826 1045 2761 3309 5014 6149 6830 7021 826 Size: 219 1716 548 1705 1135 681 191 1471 Fragments arranged by size: 1716 1705 1471 1135 681 548 219 191 SfoI GGC'GCC Cuts at: 2251 3430 4102 2251 Size: 1179 672 5815 Fragments arranged by size: 5815 1179 672 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7666 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7666 SmaI CCC'GGG Cuts at: 1872 2205 2951 3155 3377 4670 4935 4993 1872 Size: 333 746 204 222 1293 265 58 4545 Fragments arranged by size: 4545 1293 746 333 265 222 204 58 SmlI C'TyrA_G Cuts at: 820 1017 1943 2288 3352 3905 5770 6671 6933 Size: 197 926 345 1064 553 1865 901 262 Cuts at: 6933 820 Size: 1553 Fragments arranged by size: 1865 1553 1064 926 901 553 345 262 197 SnaBI TAC'GTA Cuts at: 493 5457 493 Size: 4964 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7666 SphI G_CATG'C Cuts at: 1132 4066 4165 4189 5024 1132 Size: 2934 99 24 835 3774 Fragments arranged by size: 3774 2934 835 99 24 SspI AAT'ATT Cuts at: 5 52 3725 5590 5 Size: 47 3673 1865 2081 Fragments arranged by size: 3673 2081 1865 47 StuI AGG'CCT Cuts at: 1517 3998 1517 Size: 2481 5185 StyI C'CwwG_G Cuts at: 513 1065 2004 5105 513 Size: 552 939 3101 3074 Fragments arranged by size: 3101 3074 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2089 3163 3309 3358 3397 3635 5693 Size: 1255 509 1074 146 49 39 238 2058 Cuts at: 5693 5710 5863 6048 6493 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2058 1498 1255 1074 509 445 238 185 153 146 49 39 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5912 7624 96 Size: 274 80 33 51 1443 3935 1712 138 Fragments arranged by size: 3935 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2111 2143 2720 2752 2956 2988 4680 4712 4895 Size: 32 577 32 204 32 1692 32 183 Cuts at: 4895 4927 7626 7658 2111 Size: 32 2699 32 2119 Fragments arranged by size: 2699 2119 1692 577 204 183 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3334 1316 Size: 2018 5648 XbaI T'CTAG_A Cuts at: 5002 5002 Size: 7666 XhoI C'TCGA_G Cuts at: 1943 2288 1943 Size: 345 7321 XmaI C'CCGG_G Cuts at: 1870 2203 2949 3153 3375 4668 4933 4991 1870 Size: 333 746 204 222 1293 265 58 4545 Fragments arranged by size: 4545 1293 746 333 265 222 204 58 XmnI GAAnn'nnTTC Cuts at: 5795 5795 Size: 7666 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3465 5474 276 Size: 53 83 186 936 1931 2009 2468 Fragments arranged by size: 2468 2009 1931 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BsiWI BsmBI BstZ17I EcoRV FseI NruI NsiI PacI PsrI RsrII SapI SfiI SrfI SwaI XcmI