Restriction map for
pFN21AE6459
(Circular) (Six-base) MAPSORT of: pFN21AE6459.seq Check: 9546 from: 1 to: 6064
pFN21AE6459 6064 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3150 3874 278
Size: 53 83 186 936 1614 724 2468
Fragments arranged by size:
2468 1614 936 724 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2331 3407 1937
Size: 394 1076 4594
Fragments arranged by size:
4594 1076 394
Acc65I G'GTAC_C
Cuts at: 2853 3385 2853
Size: 532 5532
AclI AA'CG_TT
Cuts at: 1563 2223 4191 4564 1563
Size: 660 1968 373 3063
Fragments arranged by size:
3063 1968 660 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2199 2218 2419 2642 3271 4127 5511 5779
Size: 567 19 201 223 629 856 1384 268
Cuts at: 5779 1632
Size: 1917
Fragments arranged by size:
1917 1384 856 629 567 268 223 201
19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 3246 4963 5940 1203
Size: 2043 1717 977 1327
Fragments arranged by size:
2043 1717 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 4793 4793
Size: 6064
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 3043 3807 5379 3043
Size: 764 1572 3728
Fragments arranged by size:
3728 1572 764
ApaI G_GGCC'C
Cuts at: 3159 3215 3159
Size: 56 6008
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 2398 2792 3373 3599 3751 5860 1493
Size: 905 394 581 226 152 2109 1697
Fragments arranged by size:
2109 1697 905 581 394 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 3015 3102 3389 1870
Size: 73 1072 87 287 4545
Fragments arranged by size:
4545 1072 287 87 73
BamHI G'GATC_C
Cuts at: 2612 3394 3761 2612
Size: 782 367 4915
Fragments arranged by size:
4915 782 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2853 3385 4840 618
Size: 325 217 627 1066 532 1455 1842
Fragments arranged by size:
1842 1455 1066 627 532 325 217
BanII G_rGCy'C
Cuts at: 1391 2100 3085 3159 3183 3215 3383 1391
Size: 709 985 74 24 32 168 4072
Fragments arranged by size:
4072 985 709 168 74 32 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5093
BbvCI CC'TCA_GC
Cuts at: 2393 2450 2393
Size: 57 6007
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4255 4289 1903
Size: 34 2318 34 3678
Fragments arranged by size:
3678 2318 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3956 5166 3956
Size: 1210 4854
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6064
BfrBI ATG'CAT
Cuts at: 2152 2152
Size: 6064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4675 136
Size: 107 122 71 631 605 3003 1525
Fragments arranged by size:
3003 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 3044 3066 3481 3044
Size: 22 415 5627
Fragments arranged by size:
5627 415 22
Bme1580I G_kGCm'C
Cuts at: 1790 3159 3215 4125 5281 1790
Size: 1369 56 910 1156 2573
Fragments arranged by size:
2573 1369 1156 910 56
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3795 4753 442
Size: 416 53 83 399 24 2378 958 1753
Fragments arranged by size:
2378 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2239 2921 3245 4724 1239
Size: 1000 682 324 1479 2579
Fragments arranged by size:
2579 1479 1000 682 324
Bpu10I CC'TnA_GC
Cuts at: 2393 2450 2926 3332 2393
Size: 57 476 406 5125
Fragments arranged by size:
5125 476 406 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2636 4189 5054 5352 2636
Size: 1553 865 298 3348
Fragments arranged by size:
3348 1553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4727 882
Size: 617 3228 2219
Fragments arranged by size:
3228 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3249 3855 493
Size: 711 2045 606 2702
Fragments arranged by size:
2702 2045 711 606
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3147 3345 3871 4253
Size: 53 83 186 936 1614 198 526 382
Cuts at: 4253 4888 275
Size: 635 1451
Fragments arranged by size:
1614 1451 936 635 526 382 198 186
83 53
BsaWI w'CCGG_w
Cuts at: 2106 4497 4895 5169 5316 5727 2106
Size: 2391 398 274 147 411 2443
Fragments arranged by size:
2443 2391 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2273 2303 2846 2876 1178
Size: 30 165 30 870 30 543 30 4366
Fragments arranged by size:
4366 870 543 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3189 1186
Size: 2003 4061
BseYI C'CCAG_C
Cuts at: 2501 3090 3170 3208 5267 5763 2501
Size: 589 80 38 2059 496 2802
Fragments arranged by size:
2802 2059 589 496 80 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2529 2654 2930 2984 1163
Size: 573 793 125 276 54 4243
Fragments arranged by size:
4243 793 573 276 125 54
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2083 3514 4275 5303 1540
Size: 275 186 82 1431 761 1028 2301
Fragments arranged by size:
2301 1431 1028 761 275 186 82
BsiHKAI G_wGCw'C
Cuts at: 2100 3254 4125 4210 5281 2100
Size: 1154 871 85 1071 2883
Fragments arranged by size:
2883 1154 1071 871 85
BsmI GAATG_Cn'
Cuts at: 3575 3668 3575
Size: 93 5971
BsmBI CGTCTCn'nnnn_
Cuts at: 2124 2124
Size: 6064
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 3085 3159 3183 3215 3254 3383
Size: 399 310 985 74 24 32 39 129
Cuts at: 3383 4125 4210 5281 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 985 742 399 310 129 85
74 39 32 24
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 3953 5683 3953
Size: 1730 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2973 3105 3419 844
Size: 2129 132 314 3489
Fragments arranged by size:
3489 2129 314 132
BsrBI CCG'CTC
Cuts at: 1394 1823 2047 2884 3951 1394
Size: 429 224 837 1067 3507
Fragments arranged by size:
3507 1067 837 429 224
BsrDI GCAATG_nn'
Cuts at: 65 1248 2323 3307 4559 4733 65
Size: 1183 1075 984 1252 174 1396
Fragments arranged by size:
1396 1252 1183 1075 984 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2931 4708 4895 1700
Size: 110 1121 1777 187 2869
Fragments arranged by size:
2869 1777 1121 187 110
BsrGI T'GTAC_A
Cuts at: 96 2368 96
Size: 2272 3792
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 4124 5136 4124
Size: 1012 5052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6064
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6064
BstXI CCAn_nnnn'nTGG
Cuts at: 2272 2272
Size: 6064
BstYI r'GATC_y
Cuts at: 1972 2612 3394 3761 4146 4163 5615 5701 5713
Size: 640 782 367 385 17 1452 86 12
Cuts at: 5713 6059 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 782 640 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2332 2332
Size: 6064
Bsu36I CC'TnA_GG
Cuts at: 2829 3275 2829
Size: 446 5618
BtgI C'CryG_G
Cuts at: 513 1065 2065 2158 2382 3175 513
Size: 552 1000 93 224 793 3402
Fragments arranged by size:
3402 1000 793 552 224 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2971 487
Size: 683 56 1745 3580
Fragments arranged by size:
3580 1745 683 56
BtsI GCAGTG_nn'
Cuts at: 2512 2918 3580 4374 4394 2512
Size: 406 662 794 20 4182
Fragments arranged by size:
4182 794 662 406 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3350 5071 809
Size: 507 2034 1721 1802
Fragments arranged by size:
2034 1802 1721 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2091 2161
Size: 54 1425 50 275 18 250 11 70
Cuts at: 2161 2262 3511 4400 6029 8
Size: 101 1249 889 1629 43
Fragments arranged by size:
1629 1425 1249 889 275 250 101 70
54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2080 3511 1537
Size: 275 268 1431 4090
Fragments arranged by size:
4090 1431 275 268
EarI CTCTTCn'nnn_
Cuts at: 1353 2105 2694 2903 3994 1353
Size: 752 589 209 1091 3423
Fragments arranged by size:
3423 1091 752 589 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4651 5025 5171 387
Size: 1064 138 3062 374 146 1280
Fragments arranged by size:
3062 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2098 2098
Size: 6064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2218 2239 2419 2642 2921 3245
Size: 393 567 19 21 180 223 279 324
Cuts at: 3245 3271 4127 4724 5511 5779 1239
Size: 26 856 597 787 268 1524
Fragments arranged by size:
1524 856 787 597 567 393 324 279
268 223 180 26 21 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2297 2621 3035 2297
Size: 324 414 5326
Fragments arranged by size:
5326 414 324
EcoO109I rG'GnC_Cy
Cuts at: 2041 3094 3155 3156 3164 3212 2041
Size: 1053 61 1 8 48 4893
Fragments arranged by size:
4893 1053 61 48 8 1
EcoRI G'AATT_C
Cuts at: 3373 3751 3373
Size: 378 5686
FspI TGC'GCA
Cuts at: 2684 4570 2684
Size: 1886 4178
FspAI rTGC'GCAy
Cuts at: 2684 2684
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 5211 1678
Size: 3533 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2573 2605 2747 2779 2861
Size: 32 135 32 1001 32 142 32 82
Cuts at: 2861 2893 2909 2941 2954 2986 3009 3041 4705
Size: 32 16 32 13 32 23 32 1664
Cuts at: 4705 4737 4779 4811 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1664 1001 142 135 82 42 32
32 32 32 32 32 32 32 32
32 23 16 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3408 3654 669
Size: 988 281 1470 246 3079
Fragments arranged by size:
3079 1470 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KpnI G_GTAC'C
Cuts at: 2857 3389 2857
Size: 532 5532
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5178 5362 1305
Size: 3873 184 2007
Fragments arranged by size:
3873 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2264 10
Size: 54 1425 343 432 3810
Fragments arranged by size:
3810 1425 432 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2157 2387 4022 4381 4540 518
Size: 648 991 230 1635 359 159 2042
Fragments arranged by size:
2042 1635 991 648 359 230 159
MspA1I CmG'CkG
Cuts at: 1606 2067 2190 3480 4157 5305 5550 1606
Size: 461 123 1290 677 1148 245 2120
Fragments arranged by size:
2120 1290 1148 677 461 245 123
NaeI GCC'GGC
Cuts at: 2933 2933
Size: 6064
NcoI C'CATG_G
Cuts at: 513 1065 2158 3175 513
Size: 552 1093 1017 3402
Fragments arranged by size:
3402 1093 1017 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NgoMIV G'CCGG_C
Cuts at: 2931 2931
Size: 6064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NsiI A_TGCA'T
Cuts at: 2154 2154
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 3422 4967 1132
Size: 97 2193 1545 2229
Fragments arranged by size:
2229 2193 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2293 3152 1348
Size: 945 859 4260
Fragments arranged by size:
4260 945 859
PciI A'CATG_T
Cuts at: 4963 4963
Size: 6064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6064
PfoI T'CCnGG_A
Cuts at: 2340 2340
Size: 6064
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PmlI CAC'GTG
Cuts at: 3249 3249
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 3094 3164 2041
Size: 1053 70 4941
Fragments arranged by size:
4941 1053 70
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6064
PsiI TTA'TAA
Cuts at: 3634 3634
Size: 6064
PspOMI G'GGCC_C
Cuts at: 3155 3211 3155
Size: 56 6008
PspXI vC'TCGA_Gb
Cuts at: 3015 3015
Size: 6064
PstI C_TGCA'G
Cuts at: 830 3416 830
Size: 2586 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
SacI G_AGCT'C
Cuts at: 2100 2100
Size: 6064
SacII CC_GC'GG
Cuts at: 2068 2068
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SanDI GG'GwC_CC
Cuts at: 2041 2041
Size: 6064
SapI GCTCTTCn'nnn_
Cuts at: 2105 2903 2105
Size: 798 5266
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SfcI C'TryA_G
Cuts at: 826 1045 3412 4547 5228 5419 826
Size: 219 2367 1135 681 191 1471
Fragments arranged by size:
2367 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2651 3015 4168 5069 5331 820
Size: 197 926 708 364 1153 901 262 1553
Fragments arranged by size:
1553 1153 926 901 708 364 262 197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 3422 1132
Size: 2290 3774
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 3132 1517
Size: 1615 4449
StyI C'CwwG_G
Cuts at: 513 1065 2034 2158 3175 3503 513
Size: 552 969 124 1017 328 3074
Fragments arranged by size:
3074 1017 969 552 328 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2934 3310 4091 4108 4261 4446 4891
Size: 1255 1354 376 781 17 153 185 445
Cuts at: 4891 325
Size: 1498
Fragments arranged by size:
1498 1354 1255 781 445 376 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2368 2593 4310
Size: 274 80 33 51 1443 391 225 1717
Cuts at: 4310 6022 96
Size: 1712 138
Fragments arranged by size:
1717 1712 1443 391 274 225 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6024 6056 6024
Size: 32 6032
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6064
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XhoI C'TCGA_G
Cuts at: 1943 3015 1943
Size: 1072 4992
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 4193 4193
Size: 6064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3148 3872 276
Size: 53 83 186 936 1614 724 2468
Fragments arranged by size:
2468 1614 936 724 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI DraIII EcoRV FalI FseI KasI NarI NruI
PacI PsrI PvuII RsrII SexAI SfiI SfoI SrfI
SwaI XcmI