(Linear) (Six-base) MAPSORT of: KIEE6459.seq Check: 9909 from: 1 to: 1359 KIEE6459.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AatII G_ACGT'C Cuts at: 0 1145 1359 Size: 1145 214 AccI GT'mk_AC Cuts at: 0 326 1359 Size: 326 1033 Acc65I G'GTAC_C Cuts at: 0 848 1359 Size: 848 511 AclI AA'CG_TT Cuts at: 0 218 1359 Size: 218 1141 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 194 213 414 637 1266 1359 Size: 194 19 201 223 629 93 Fragments arranged by size: 629 223 201 194 93 19 AflIII A'CryG_T Cuts at: 0 1241 1359 Size: 1241 118 AlwNI CAG_nnn'CTG Cuts at: 0 1038 1359 Size: 1038 321 ApaI G_GGCC'C Cuts at: 0 1154 1210 1359 Size: 1154 56 149 Fragments arranged by size: 1154 149 56 ApoI r'AATT_y Cuts at: 0 393 787 1359 Size: 393 394 572 Fragments arranged by size: 572 394 393 AvaI C'yCGr_G Cuts at: 0 1010 1097 1359 Size: 1010 87 262 Fragments arranged by size: 1010 262 87 BamHI G'GATC_C Cuts at: 0 607 1359 Size: 607 752 BanI G'GyrC_C Cuts at: 0 848 1359 Size: 848 511 BanII G_rGCy'C Cuts at: 0 95 1080 1154 1178 1210 1359 Size: 95 985 74 24 32 149 Fragments arranged by size: 985 149 95 74 32 24 BbvCI CC'TCA_GC Cuts at: 0 388 445 1359 Size: 388 57 914 Fragments arranged by size: 914 388 57 BfrBI ATG'CAT Cuts at: 0 147 1359 Size: 147 1212 BlpI GC'TnA_GC Cuts at: 0 1039 1061 1359 Size: 1039 22 298 Fragments arranged by size: 1039 298 22 Bme1580I G_kGCm'C Cuts at: 0 1154 1210 1359 Size: 1154 56 149 Fragments arranged by size: 1154 149 56 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 234 916 1240 1359 Size: 234 682 324 119 Fragments arranged by size: 682 324 234 119 Bpu10I CC'TnA_GC Cuts at: 0 388 445 921 1327 1359 Size: 388 57 476 406 32 Fragments arranged by size: 476 406 388 57 32 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 631 1359 Size: 631 728 BsaAI yAC'GTr Cuts at: 0 1244 1359 Size: 1244 115 BsaHI Gr'CG_yC Cuts at: 0 1142 1340 1359 Size: 1142 198 19 Fragments arranged by size: 1142 198 19 BsaWI w'CCGG_w Cuts at: 0 101 1359 Size: 101 1258 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 268 298 841 871 1359 Size: 268 30 543 30 488 Fragments arranged by size: 543 488 268 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1184 1359 Size: 1184 175 BseYI C'CCAG_C Cuts at: 0 496 1085 1165 1203 1359 Size: 496 589 80 38 156 Fragments arranged by size: 589 496 156 80 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 524 649 925 979 1359 Size: 524 125 276 54 380 Fragments arranged by size: 524 380 276 125 54 BsiEI CG_ry'CG Cuts at: 0 78 1359 Size: 78 1281 BsiHKAI G_wGCw'C Cuts at: 0 95 1249 1359 Size: 95 1154 110 Fragments arranged by size: 1154 110 95 BsmBI CGTCTCn'nnnn_ Cuts at: 0 119 1359 Size: 119 1240 Bsp1286I G_dGCh'C Cuts at: 0 95 1080 1154 1178 1210 1249 1359 Size: 95 985 74 24 32 39 110 Fragments arranged by size: 985 110 95 74 39 32 24 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 968 1100 1359 Size: 968 132 259 Fragments arranged by size: 968 259 132 BsrBI CCG'CTC Cuts at: 0 42 879 1359 Size: 42 837 480 Fragments arranged by size: 837 480 42 BsrDI GCAATG_nn' Cuts at: 0 318 1302 1359 Size: 318 984 57 Fragments arranged by size: 984 318 57 BsrFI r'CCGG_y Cuts at: 0 926 1359 Size: 926 433 BsrGI T'GTAC_A Cuts at: 0 363 1359 Size: 363 996 BstXI CCAn_nnnn'nTGG Cuts at: 0 267 1359 Size: 267 1092 BstYI r'GATC_y Cuts at: 0 607 1359 Size: 607 752 BstZ17I GTA'TAC Cuts at: 0 327 1359 Size: 327 1032 Bsu36I CC'TnA_GG Cuts at: 0 824 1270 1359 Size: 824 446 89 Fragments arranged by size: 824 446 89 BtgI C'CryG_G Cuts at: 0 60 153 377 1170 1359 Size: 60 93 224 793 189 Fragments arranged by size: 793 224 189 93 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 966 1359 Size: 966 393 BtsI GCAGTG_nn' Cuts at: 0 507 913 1359 Size: 507 406 446 Fragments arranged by size: 507 446 406 DrdI GACnn_nn'nnGTC Cuts at: 0 1345 1359 Size: 1345 14 EaeI y'GGCC_r Cuts at: 0 75 86 156 257 1359 Size: 75 11 70 101 1102 Fragments arranged by size: 1102 101 75 70 11 EagI C'GGCC_G Cuts at: 0 75 1359 Size: 75 1284 EarI CTCTTCn'nnn_ Cuts at: 0 100 689 898 1359 Size: 100 589 209 461 Fragments arranged by size: 589 461 209 100 EcoICRI GAG'CTC Cuts at: 0 93 1359 Size: 93 1266 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 194 213 234 414 637 916 1240 1266 Size: 194 19 21 180 223 279 324 26 Cuts at: 1266 1359 Size: 93 Fragments arranged by size: 324 279 223 194 180 93 26 21 19 EcoNI CCTnn'n_nnAGG Cuts at: 0 292 616 1030 1359 Size: 292 324 414 329 Fragments arranged by size: 414 329 324 292 EcoO109I rG'GnC_Cy Cuts at: 0 36 1089 1150 1151 1159 1207 1359 Size: 36 1053 61 1 8 48 152 Fragments arranged by size: 1053 152 61 48 36 8 1 FspI TGC'GCA Cuts at: 0 679 1359 Size: 679 680 FspAI rTGC'GCAy Cuts at: 0 679 1359 Size: 679 680 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 568 600 742 774 856 888 904 936 Size: 568 32 142 32 82 32 16 32 Cuts at: 936 949 981 1004 1036 1359 Size: 13 32 23 32 323 Fragments arranged by size: 568 323 142 82 32 32 32 32 32 32 23 16 13 KpnI G_GTAC'C Cuts at: 0 852 1359 Size: 852 507 MscI TGG'CCA Cuts at: 0 259 1359 Size: 259 1100 MslI CAynn'nnrTG Cuts at: 0 152 382 1359 Size: 152 230 977 Fragments arranged by size: 977 230 152 MspA1I CmG'CkG Cuts at: 0 62 185 1359 Size: 62 123 1174 Fragments arranged by size: 1174 123 62 NaeI GCC'GGC Cuts at: 0 928 1359 Size: 928 431 NcoI C'CATG_G Cuts at: 0 153 1170 1359 Size: 153 1017 189 Fragments arranged by size: 1017 189 153 NgoMIV G'CCGG_C Cuts at: 0 926 1359 Size: 926 433 NsiI A_TGCA'T Cuts at: 0 149 1359 Size: 149 1210 PasI CC'CwG_GG Cuts at: 0 288 1147 1359 Size: 288 859 212 Fragments arranged by size: 859 288 212 PfoI T'CCnGG_A Cuts at: 0 335 1359 Size: 335 1024 PmlI CAC'GTG Cuts at: 0 1244 1359 Size: 1244 115 PpuMI rG'GwC_Cy Cuts at: 0 36 1089 1159 1359 Size: 36 1053 70 200 Fragments arranged by size: 1053 200 70 36 PspOMI G'GGCC_C Cuts at: 0 1150 1206 1359 Size: 1150 56 153 Fragments arranged by size: 1150 153 56 PspXI vC'TCGA_Gb Cuts at: 0 1010 1359 Size: 1010 349 SacI G_AGCT'C Cuts at: 0 95 1359 Size: 95 1264 SacII CC_GC'GG Cuts at: 0 63 1359 Size: 63 1296 SanDI GG'GwC_CC Cuts at: 0 36 1359 Size: 36 1323 SapI GCTCTTCn'nnn_ Cuts at: 0 100 898 1359 Size: 100 798 461 Fragments arranged by size: 798 461 100 SmlI C'TyrA_G Cuts at: 0 646 1010 1359 Size: 646 364 349 Fragments arranged by size: 646 364 349 StuI AGG'CCT Cuts at: 0 1127 1359 Size: 1127 232 StyI C'CwwG_G Cuts at: 0 29 153 1170 1359 Size: 29 124 1017 189 Fragments arranged by size: 1017 189 124 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 929 1305 1359 Size: 929 376 54 Fragments arranged by size: 929 376 54 TatI w'GTAC_w Cuts at: 0 363 588 1359 Size: 363 225 771 Fragments arranged by size: 771 363 225 XhoI C'TCGA_G Cuts at: 0 1010 1359 Size: 1010 349 ZraI GAC'GTC Cuts at: 0 1143 1359 Size: 1143 216 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApoI AvaI BamHI BanI BanII BbvCI BfrBI BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI Hin4I KpnI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI PasI PfoI PmlI PpuMI PspOMI PspXI SacI SacII SanDI SapI SmlI StuI StyI TaqII TatI XhoI ZraI Enzymes that do not cut: AarI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI BcgI BciVI BclI BglI BglII BmgBI BmrI BmtI BplI BsaI BsaBI BsiWI BsmI BspEI BspHI BssHII BssSI BstAPI BstBI BstEII ClaI CspCI DraI DraIII EciI EcoRI EcoRV FalI FseI HaeII HincII HindIII HpaI KasI MfeI MluI MmeI NarI NdeI NheI NotI NruI NspI PacI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII RsrII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI XmaI XmnI