Restriction map for
pFN21AE5642
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# NO CUT ENZYMES ( 138 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BbeI BbrPI BbvI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I Fnu4HI FseI FspI HaeII
HgaI HhaI HinP1I HpaI ItaI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI TseI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 520 GACGTC GACGT^C
AciI 483 GCGG CCGC(-3/-1)
AcyI 520 GACGTC G[GA]^CG[CT]C
AfaI 1218 GTAC GT^AC
ApaI 118 GGGCCC GGGCC^C
Asp700I 77 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 648 GGATCC G^GATCC
BbsI 301 GTCTTC GAAGAC(2/6)
BbvCI 361 GCTGAGG CCTCAGC(-5/-2)
BpmI 877 CTGGAG CTGGAG(16/14)
BpuAI 301 GTCTTC GAAGAC(2/6)
BsaBI 383 GATTACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 520 GACGTC G[GA]^CG[CT]C
BsaWI 311 ACCGGA [AT]^CCGG[AT]
BseRI 61 CTCCTC GAGGAG(10/8)
BspHI 1346 TCATGA T^CATGA
BstAPI 984 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 799 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 23 CCATGG C^C[GA][CT]GG
EcoT14I 23 CCATGG C^C[AT][AT]GG
FauI 483 GCGGG CCCGC(4/6)
HapII 312 CCGG C^CGG
Hin1I 520 GACGTC G[GA]^CG[CT]C
HincII 230 GTCAAC GT[CT]^[GA]AC
HindII 230 GTCAAC GT[CT]^[GA]AC
HpaII 312 CCGG C^CGG
MaeII 521 ACGT A^CGT
MamI 383 GATTACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1103 CAATTG C^AATTG
MspA1I 817 CAGCTG C[AC]G^C[GT]G
MspI 312 CCGG C^CGG
MunI 1103 CAATTG C^AATTG
MwoI 984 GCAGAAACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 23 CCATGG C^CATGG
PstI 991 CTGCAG CTGCA^G
PvuII 817 CAGCTG CAG^CTG
RcaI 1346 TCATGA T^CATGA
RsaI 1218 GTAC GT^AC
SacI 880 GAGCTC GAGCT^C
SapI 1198 GCTCTTC GCTCTTC(1/4)
ScaI 1217 AGTACT AGT^ACT
SfaNI 1006 GATGC GCATC(5/9)
SspI 145 AATATT AAT^ATT
StuI 316 AGGCCT AGG^CCT
StyI 23 CCATGG C^C[AT][AT]GG
XcmI 1166 CCAAAGGGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 77 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AspHI 58 GTGCTC G[AT]GC[AT]^C
880 GAGCTC
BalI 20 TGGCCA TGG^CCA
962 TGGCCA
BanI 1026 GGCACC G^G[CT][GA]CC
1492 GGCACC
BanII 118 GGGCCC G[GA]GC[CT]^C
880 GAGCTC
BclI 266 TGATCA T^GATCA
548 TGATCA
BfaI 280 CTAG C^TAG
554 CTAG
BsaJI 23 CCATGG C^C[ACGT][ACGT]GG
115 CCAGGG
BsiHKAI 58 GTGCTC G[AT]GC[AT]^C
880 GAGCTC
BsmFI 436 GTCCC GGGAC(10/14)
855 GTCCC
BsrDI 392 CATTGC GCAATG(2/0)
444 CATTGC
BssSI 927 CACGAG CACGAG(-5/-1)
883 CTCGTG
DraII 117 AGGGCCC [GA]G^G[ACGT]CC[CT]
529 GGGGCCT
EaeI 20 TGGCCA [CT]^GGCC[GA]
962 TGGCCA
EarI 1199 CTCTTC CTCTTC(1/4)
378 GAAGAG
EcoO109I 117 AGGGCCC [GA]G^G[ACGT]CC[CT]
529 GGGGCCT
EcoRI 110 GAATTC G^AATTC
837 GAATTC
FbaI 266 TGATCA T^GATCA
548 TGATCA
Ksp632I 1199 CTCTTC CTCTTC(1/4)
378 GAAGAG
MaeI 280 CTAG C^TAG
554 CTAG
MluNI 20 TGGCCA TGG^CCA
962 TGGCCA
MscI 20 TGGCCA TGG^CCA
962 TGGCCA
SmlI 424 CTCAAG C^T[CT][GA]AG
1117 CTTGAG
TaqI 578 TCGA T^CGA
782 TCGA
TfiI 1185 GATTC G^A[AT]TC
1253 GATTC
Tsp45I 508 GTCAC ^GT[GC]AC
1208 GTGAC
TthHB8I 578 TCGA T^CGA
782 TCGA
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AluI AlwI AlwNI ApoI
AvaII BmyI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII FokI HaeIII HindIII
HinfI HphI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfcI Tru9I Tsp509I
TspRI XhoII