(Circular) (Six-base) MAPSORT of: pFN21AE5642.seq Check: 181 from: 1 to: 6214 pFN21AE5642 6214 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2529 4024 278 Size: 53 83 186 936 993 1495 2468 Fragments arranged by size: 2468 1495 993 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3557 1937 Size: 1620 4594 Acc65I G'GTAC_C Cuts at: 3535 3535 Size: 6214 AclI AA'CG_TT Cuts at: 1563 4341 4714 1563 Size: 2778 373 3063 Fragments arranged by size: 3063 2778 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2804 3368 4277 5661 5929 1632 Size: 1172 564 909 1384 268 1917 Fragments arranged by size: 1917 1384 1172 909 564 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6017 AflIII A'CryG_T Cuts at: 1203 5113 6090 1203 Size: 3910 977 1327 Fragments arranged by size: 3910 1327 977 AgeI A'CCGG_T Cuts at: 5045 5045 Size: 6214 AhdI GACnn_n'nnGTC Cuts at: 4943 4943 Size: 6214 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6214 AlwNI CAG_nnn'CTG Cuts at: 2264 2995 3236 3327 3957 5529 2264 Size: 731 241 91 630 1572 2949 Fragments arranged by size: 2949 1572 731 630 241 91 ApaI G_GGCC'C Cuts at: 2127 2127 Size: 6214 ApaLI G'TGCA_C Cuts at: 4271 5427 4271 Size: 1156 5058 ApoI r'AATT_y Cuts at: 1493 2115 2743 2842 3523 3749 3901 6010 1493 Size: 622 628 99 681 226 152 2109 1697 Fragments arranged by size: 2109 1697 681 628 622 226 152 99 AseI AT'TA_AT Cuts at: 160 1032 4768 160 Size: 872 3736 1606 Fragments arranged by size: 3736 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 AvaI C'yCGr_G Cuts at: 1870 1943 3539 1870 Size: 73 1596 4545 Fragments arranged by size: 4545 1596 73 BamHI G'GATC_C Cuts at: 2653 3544 3911 2653 Size: 891 367 4956 Fragments arranged by size: 4956 891 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3031 3497 3535 4990 618 Size: 325 217 627 1244 466 38 1455 1842 Fragments arranged by size: 1842 1455 1244 627 466 325 217 38 BanII G_rGCy'C Cuts at: 1391 2127 2889 3533 1391 Size: 736 762 644 4072 Fragments arranged by size: 4072 762 736 644 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2299 928 Size: 971 400 4843 Fragments arranged by size: 4843 971 400 BbvCI CC'TCA_GC Cuts at: 2367 2367 Size: 6214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4405 4439 1903 Size: 34 2468 34 3678 Fragments arranged by size: 3678 2468 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4106 5316 4106 Size: 1210 5004 BclI T'GATC_A Cuts at: 1548 2271 2553 1548 Size: 723 282 5209 Fragments arranged by size: 5209 723 282 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4825 136 Size: 107 122 71 631 605 3153 1525 Fragments arranged by size: 3153 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6209 6209 Size: 6214 BlpI GC'TnA_GC Cuts at: 3631 3631 Size: 6214 Bme1580I G_kGCm'C Cuts at: 1790 2127 4275 5431 1790 Size: 337 2148 1156 2573 Fragments arranged by size: 2573 2148 1156 337 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6214 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2449 3945 4903 Size: 416 53 83 399 24 1032 1496 958 Cuts at: 4903 442 Size: 1753 Fragments arranged by size: 1753 1496 1032 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6214 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3363 3363 Size: 6214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2903 4874 1239 Size: 1664 1971 2579 Fragments arranged by size: 2579 1971 1664 Bpu10I CC'TnA_GC Cuts at: 2367 3439 2367 Size: 1072 5142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2414 3143 4339 5204 5502 2414 Size: 729 1196 865 298 3126 Fragments arranged by size: 3126 1196 865 729 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4877 882 Size: 617 3378 2219 Fragments arranged by size: 3378 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4005 493 Size: 711 2801 2702 Fragments arranged by size: 2801 2702 711 BsaBI GATnn'nnATC Cuts at: 2392 6208 2392 Size: 3816 2398 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2526 4021 4403 5038 Size: 53 83 186 936 993 1495 382 635 Cuts at: 5038 275 Size: 1451 Fragments arranged by size: 1495 1451 993 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2316 4647 5045 5319 5466 5877 2316 Size: 2331 398 274 147 411 2653 Fragments arranged by size: 2653 2331 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5989 Fragments arranged by size: 5989 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 1186 Size: 871 5343 BseYI C'CCAG_C Cuts at: 5417 5913 5417 Size: 496 5718 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5641 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3664 4425 5453 1540 Size: 275 186 1663 761 1028 2301 Fragments arranged by size: 2301 1663 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2067 2889 4275 4360 5431 2067 Size: 822 1386 85 1071 2850 Fragments arranged by size: 2850 1386 1071 822 85 BsmI GAATG_Cn' Cuts at: 3725 3818 3725 Size: 93 6121 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2127 2889 3533 4275 4360 5431 Size: 399 277 60 762 644 742 85 1071 Cuts at: 5431 1391 Size: 2174 Fragments arranged by size: 2174 1071 762 742 644 399 277 85 60 BspEI T'CCGG_A Cuts at: 5877 5877 Size: 6214 BspHI T'CATG_A Cuts at: 3351 4103 5833 3351 Size: 752 1730 3732 Fragments arranged by size: 3732 1730 752 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3569 844 Size: 2725 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4101 1394 Size: 429 2278 3507 Fragments arranged by size: 3507 2278 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2396 2448 4709 4883 65 Size: 1183 1148 52 2261 174 1396 Fragments arranged by size: 2261 1396 1183 1148 174 52 BsrFI r'CCGG_y Cuts at: 1700 1810 4858 5045 1700 Size: 110 3048 187 2869 Fragments arranged by size: 3048 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6214 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6214 BssSI C'ACGA_G Cuts at: 2888 2932 4274 5286 2888 Size: 44 1342 1012 3816 Fragments arranged by size: 3816 1342 1012 44 BstAPI GCAn_nnn'nTGC Cuts at: 794 2995 794 Size: 2201 4013 BstBI TT'CG_AA Cuts at: 5018 5018 Size: 6214 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6214 BstXI CCAn_nnnn'nTGG Cuts at: 2093 3178 3225 2093 Size: 1085 47 5082 Fragments arranged by size: 5082 1085 47 BstYI r'GATC_y Cuts at: 1972 2260 2653 3286 3420 3544 3911 4296 4313 Size: 288 393 633 134 124 367 385 17 Cuts at: 4313 5765 5851 5863 6209 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 633 393 385 367 346 288 134 124 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 513 Size: 552 939 24 4699 Fragments arranged by size: 4699 939 552 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5475 Fragments arranged by size: 5475 683 56 BtsI GCAGTG_nn' Cuts at: 2944 3730 4524 4544 2944 Size: 786 794 20 4614 Fragments arranged by size: 4614 794 786 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6179 DraI TTT'AAA Cuts at: 3518 3518 Size: 6214 DraIII CAC_nnn'GTG Cuts at: 2809 2809 Size: 6214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5221 809 Size: 507 3905 1802 Fragments arranged by size: 3905 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2967 3661 Size: 54 1425 50 275 18 195 942 694 Cuts at: 3661 4550 6179 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 942 889 694 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3661 1537 Size: 275 1849 4090 Fragments arranged by size: 4090 1849 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2378 3210 4144 1353 Size: 1025 832 934 3423 Fragments arranged by size: 3423 1025 934 832 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4801 5175 5321 387 Size: 1064 138 3212 374 146 1280 Fragments arranged by size: 3212 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2887 2887 Size: 6214 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2804 2903 3368 4277 4874 5661 5929 Size: 393 1172 99 465 909 597 787 268 Cuts at: 5929 1239 Size: 1524 Fragments arranged by size: 1524 1172 909 787 597 465 393 268 99 EcoO109I rG'GnC_Cy Cuts at: 2123 2535 2123 Size: 412 5802 EcoRI G'AATT_C Cuts at: 2115 2842 3523 3901 2115 Size: 727 681 378 4428 Fragments arranged by size: 4428 727 681 378 FspI TGC'GCA Cuts at: 4720 4720 Size: 6214 HaeII r_GCGC'y Cuts at: 1678 5361 1678 Size: 3683 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2565 2597 2821 2853 3052 Size: 32 135 32 993 32 224 32 199 Cuts at: 3052 3084 4855 4887 4929 4961 1373 Size: 32 1771 32 42 32 2626 Fragments arranged by size: 2626 1771 993 224 199 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2237 3558 3804 669 Size: 988 281 299 1321 246 3079 Fragments arranged by size: 3079 1321 988 299 281 246 HindIII A'AGCT_T Cuts at: 748 2108 2630 3436 748 Size: 1360 522 806 3526 Fragments arranged by size: 3526 1360 806 522 HpaI GTT'AAC Cuts at: 3804 3804 Size: 6214 KpnI G_GTAC'C Cuts at: 3539 3539 Size: 6214 MfeI C'AATT_G Cuts at: 3108 3813 3108 Size: 705 5509 MluI A'CGCG_T Cuts at: 6090 6090 Size: 6214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2432 5328 5512 1305 Size: 1127 2896 184 2007 Fragments arranged by size: 2896 2007 1127 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 2969 10 Size: 54 1425 343 195 942 3255 Fragments arranged by size: 3255 1425 942 343 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2091 2549 2979 3080 3335 3356 4172 Size: 648 925 458 430 101 255 21 816 Cuts at: 4172 4531 4690 518 Size: 359 159 2042 Fragments arranged by size: 2042 925 816 648 458 430 359 255 159 101 21 MspA1I CmG'CkG Cuts at: 1606 2824 3630 4307 5455 5700 1606 Size: 1218 806 677 1148 245 2120 Fragments arranged by size: 2120 1218 1148 806 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2028 513 Size: 552 939 24 4699 Fragments arranged by size: 4699 939 552 24 NdeI CA'TA_TG Cuts at: 387 387 Size: 6214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6214 NotI GC'GGCC_GC Cuts at: 3661 3661 Size: 6214 NspI r_CATG'y Cuts at: 1132 1229 3572 5117 1132 Size: 97 2343 1545 2229 Fragments arranged by size: 2343 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6214 PciI A'CATG_T Cuts at: 5113 5113 Size: 6214 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6214 PmeI GTTT'AAAC Cuts at: 3518 3518 Size: 6214 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2570 2602 4283 4315 5796 5828 2570 Size: 32 1681 32 1481 32 2956 Fragments arranged by size: 2956 1681 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6214 PsiI TTA'TAA Cuts at: 3784 3784 Size: 6214 PspOMI G'GGCC_C Cuts at: 2123 2123 Size: 6214 PstI C_TGCA'G Cuts at: 830 3000 3566 830 Size: 2170 566 3478 Fragments arranged by size: 3478 2170 566 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6214 PvuII CAG'CTG Cuts at: 2824 2824 Size: 6214 SacI G_AGCT'C Cuts at: 2889 2889 Size: 6214 SalI G'TCGA_C Cuts at: 1936 3556 1936 Size: 1620 4594 SapI GCTCTTCn'nnn_ Cuts at: 3210 3210 Size: 6214 SbfI CC_TGCA'GG Cuts at: 3566 3566 Size: 6214 ScaI AGT'ACT Cuts at: 3224 4462 6174 3224 Size: 1238 1712 3264 Fragments arranged by size: 3264 1712 1238 SfcI C'TryA_G Cuts at: 826 1045 2361 2539 2996 3056 3562 4697 5378 Size: 219 1316 178 457 60 506 1135 681 Cuts at: 5378 5569 826 Size: 191 1471 Fragments arranged by size: 1471 1316 1135 681 506 457 219 191 178 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6214 SmaI CCC'GGG Cuts at: 1872 3541 1872 Size: 1669 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2429 3122 4318 5219 5481 820 Size: 197 926 486 693 1196 901 262 1553 Fragments arranged by size: 1553 1196 926 901 693 486 262 197 SnaBI TAC'GTA Cuts at: 493 4005 493 Size: 3512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6214 SphI G_CATG'C Cuts at: 1132 3572 1132 Size: 2440 3774 SspI AAT'ATT Cuts at: 5 52 2152 4138 5 Size: 47 2100 1986 2081 Fragments arranged by size: 2100 2081 1986 47 StuI AGG'CCT Cuts at: 1517 2323 1517 Size: 806 5408 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 3653 513 Size: 552 939 24 1625 3074 Fragments arranged by size: 3074 1625 939 552 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3052 4241 4258 4411 4596 5041 325 Size: 1255 1472 1189 17 153 185 445 1498 Fragments arranged by size: 1498 1472 1255 1189 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3222 4460 6172 Size: 274 80 33 51 1443 1245 1238 1712 Cuts at: 6172 96 Size: 138 Fragments arranged by size: 1712 1443 1245 1238 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6174 6206 6174 Size: 32 6182 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6214 XbaI T'CTAG_A Cuts at: 3550 3550 Size: 6214 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3178 3178 Size: 6214 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6214 XmaI C'CCGG_G Cuts at: 1870 3539 1870 Size: 1669 4545 XmnI GAAnn'nnTTC Cuts at: 2086 4343 2086 Size: 2257 3957 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2527 4022 276 Size: 53 83 186 936 993 1495 2468 Fragments arranged by size: 2468 1495 993 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI