################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvI BcgI BciVI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI ItaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TseI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflIII 519 ACACGT A^C[GA][CT]GT ApaI 118 GGGCCC GGGCC^C AspHI 880 GAGCTC G[AT]GC[AT]^C BalI 962 TGGCCA TGG^CCA BanI 1026 GGCACC G^G[CT][GA]CC BbsI 301 GTCTTC GAAGAC(2/6) BbvCI 361 GCTGAGG CCTCAGC(-5/-2) BglI 318 GCCTTTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 877 CTGGAG CTGGAG(16/14) BpuAI 301 GTCTTC GAAGAC(2/6) BsaWI 311 ACCGGA [AT]^CCGG[AT] BseRI 61 CTCCTC GAGGAG(10/8) BsgI 991 GTGCAG GTGCAG(16/14) BsiHKAI 880 GAGCTC G[AT]GC[AT]^C BsmFI 436 GTCCC GGGAC(10/14) BspHI 1206 TCATGA T^CATGA BstAPI 984 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 799 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 23 CCGTGG C^C[GA][CT]GG EarI 378 GAAGAG CTCTTC(1/4) FauI 483 GCGGG CCCGC(4/6) HapII 312 CCGG C^CGG HincII 230 GTCAAC GT[CT]^[GA]AC HindII 230 GTCAAC GT[CT]^[GA]AC HpaII 312 CCGG C^CGG Ksp632I 378 GAAGAG CTCTTC(1/4) MaeIII 508 GTCAC ^GT[ACGT]AC MluNI 962 TGGCCA TGG^CCA MscI 962 TGGCCA TGG^CCA MspI 312 CCGG C^CGG PvuII 817 CAGCTG CAG^CTG RcaI 1206 TCATGA T^CATGA SacI 880 GAGCTC GAGCT^C ScaI 1217 AGTACT AGT^ACT SfaNI 575 GCATC GCATC(5/9) SfiI 317 GGCCTTTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 316 AGGCCT AGG^CCT TaqI 578 TCGA T^CGA TfiI 1185 GATTC G^A[AT]TC Tsp45I 508 GTCAC ^GT[GC]AC TthHB8I 578 TCGA T^CGA XcmI 1166 CCAAAGGGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 416 GCGG CCGC(-3/-1) 483 GCGG AcsI 110 GAATTC [GA]^AATT[CT] 837 GAATTC AfaI 83 GTAC GT^AC 1218 GTAC AlwNI 1226 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1317 CAGAAACTG ApoI 110 GAATTC [GA]^AATT[CT] 837 GAATTC AvaII 435 GGTCC G^G[AT]CC 1312 GGACC BanII 118 GGGCCC G[GA]GC[CT]^C 880 GAGCTC BclI 266 TGATCA T^GATCA 548 TGATCA BmyI 118 GGGCCC G[AGT]GC[ACT]^C 880 GAGCTC BsaI 853 GAGACC GGTCTC(1/5) 1492 GAGACC BsaJI 23 CCGTGG C^C[ACGT][ACGT]GG 115 CCAGGG Bsp1286I 118 GGGCCC G[AGT]GC[ACT]^C 880 GAGCTC BsrDI 392 CATTGC GCAATG(2/0) 444 CATTGC BssSI 927 CACGAG CACGAG(-5/-1) 1345 CTCGTG BstXI 1166 CCAAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1213 CCAAAGTACTGG DraII 117 AGGGCCC [GA]G^G[ACGT]CC[CT] 529 GGGGCCT EaeI 20 TGGCCG [CT]^GGCC[GA] 962 TGGCCA EcoO109I 117 AGGGCCC [GA]G^G[ACGT]CC[CT] 529 GGGGCCT EcoRI 110 GAATTC G^AATTC 837 GAATTC FbaI 266 TGATCA T^GATCA 548 TGATCA HindIII 103 AAGCTT A^AGCTT 625 AAGCTT MaeII 175 ACGT A^CGT 521 ACGT MspA1I 414 CAGCGG C[AC]G^C[GT]G 817 CAGCTG MwoI 318 GCCTTTCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 984 GCAGAAAGTGC RsaI 83 GTAC GT^AC 1218 GTAC SmlI 424 CTCAAG C^T[CT][GA]AG 1117 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AluI AlwI BfaI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII FokI HaeIII HinfI HphI MaeI MboI MboII MflI MnlI MseI MslI MvaI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfcI Tru9I Tsp509I TspRI XhoII