Restriction map for
pFN21AE2073
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII AvrII
BbeI BbrPI BciVI BclI BcnI
BglI BlnI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmFI Bsp1407I
BspDI BspEI BspMI BsrDI BsrFI
BsrGI BssHII BstEII BstPI BstUI
BstXI Bsu36I Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HapII Hin1I HindIII HpaI HpaII
KasI KpnI KspI MamI MluI
MroI MspA1I MspI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SapI
Sau96I SbfI SexAI SfiI SfoI
SgrAI SmaI SpeI Sse8387I SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 717 GTATAC GT^[AC][GT]AC
AciI 552 GCGG CCGC(-3/-1)
AflII 2041 CTTAAG C^TTAAG
AflIII 1446 ACATGT A^C[GA][CT]GT
Alw44I 632 GTGCAC G^TGCAC
AlwNI 1634 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 632 GTGCAC G^TGCAC
Asp700I 1258 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 74 CTCGGG C^[CT]CG[GA]G
BalI 1459 TGGCCA TGG^CCA
BamHI 1491 GGATCC G^GATCC
BanII 767 GAGCTC G[GA]GC[CT]^C
BcgI 1443 GCAACATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2041 CTTAAG C^TTAAG
BlpI 1645 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1645 GCTAAGC GC^T[ACGT]AGC
BsaAI 211 TACGTA [CT]AC^GT[GA]
BsaI 2146 GGTCTC GGTCTC(1/5)
BsgI 1330 GTGCAG GTGCAG(16/14)
BsmBI 1354 CGTCTC CGTCTC(1/5)
BsmI 1349 GAATGC GAATGC(1/-1)
BsoBI 74 CTCGGG C^[CT]CG[GA]G
BspHI 1806 TCATGA T^CATGA
BspLU11I 1446 ACATGT A^CATGT
BsrBI 550 GAGCGG CCGCTC(-3/-3)
BssSI 2149 CTCGTG CACGAG(-5/-1)
Bst1107I 717 GTATAC GTA^TAC
BstAPI 28 GCATATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1265 TTCGAA TT^CGAA
BstZ17I 717 GTATAC GTA^TAC
CelII 1645 GCTAAGC GC^T[ACGT]AGC
DraI 1050 TTTAAA TTT^AAA
EaeI 1459 TGGCCA [CT]^GGCC[GA]
HaeII 883 AGCGCC [GA]GCGC^[CT]
HgaI 1353 GCGTC GACGC(5/10)
HincII 940 GTCAAC GT[CT]^[GA]AC
HindII 940 GTCAAC GT[CT]^[GA]AC
HphI 1857 TCACC GGTGA(8/7)
MfeI 2065 CAATTG C^AATTG
MluNI 1459 TGGCCA TGG^CCA
MscI 1459 TGGCCA TGG^CCA
MunI 2065 CAATTG C^AATTG
NspV 1265 TTCGAA TT^CGAA
PleI 419 GAGTC GAGTC(4/5)
RcaI 1806 TCATGA T^CATGA
SacI 767 GAGCTC GAGCT^C
ScaI 436 AGTACT AGT^ACT
SfuI 1265 TTCGAA TT^CGAA
SnaBI 211 TACGTA TAC^GTA
SphI 982 GCATGC GCATG^C
TaqI 1266 TCGA T^CGA
TthHB8I 1266 TCGA T^CGA
XmnI 1258 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AspHI 632 GTGCAC G[AT]GC[AT]^C
767 GAGCTC
BanI 966 GGCACC G^G[CT][GA]CC
1779 GGCACC
BbvCI 1407 CCTCAGC CCTCAGC(-5/-2)
1510 CCTCAGC
BglII 708 AGATCT A^GATCT
1168 AGATCT
BpmI 770 CTCCAG CTGGAG(16/14)
1088 CTGGAG
BsiHKAI 632 GTGCAC G[AT]GC[AT]^C
767 GAGCTC
BssKI 249 CCAGG ^CC[ACGT]GG
379 CCAGG
BstNI 249 CCAGG CC^[AT]GG
379 CCAGG
Cac8I 982 GCATGC GC[ACGT]^[ACGT]GC
1942 GCAAGC
CfoI 884 GCGC GCG^C
1866 GCGC
EarI 1073 CTCTTC CTCTTC(1/4)
1526 GAAGAG
EcoRII 249 CCAGG ^CC[AT]GG
379 CCAGG
EcoT14I 476 CCTTGG C^C[AT][AT]GG
1544 CCTTGG
HaeIII 1005 GGCC GG^CC
1460 GGCC
HhaI 884 GCGC GCG^C
1866 GCGC
HinP1I 884 GCGC G^CGC
1866 GCGC
Ksp632I 1073 CTCTTC CTCTTC(1/4)
1526 GAAGAG
MaeII 212 ACGT A^CGT
498 ACGT
MslI 1447 CATGTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1965 CATCCCTGTG
MvaI 249 CCAGG CC^[AT]GG
379 CCAGG
MwoI 28 GCATATGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1636 GCTCCTGATGC
PflMI 751 CCATACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2097 CCATAGCATGG
ScrFI 249 CCAGG CC^[ACGT]GG
379 CCAGG
SfcI 423 CTGTAG C^T[GA][CT]AG
627 CTATAG
SspI 1180 AATATT AAT^ATT
1376 AATATT
StyI 476 CCTTGG C^C[AT][AT]GG
1544 CCTTGG
Van91I 751 CCATACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2097 CCATAGCATGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BbvI BfaI BmyI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrI BstF5I BstYI DdeI
DpnII EcoT22I Fnu4HI FokI HinfI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI NdeI NdeII
NlaIII NlaIV NsiI NspI RsaI
Sau3AI SfaNI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII