(Circular) (Six-base) MAPSORT of: pFN21AE2073.seq Check: 5503 from: 1 to: 6913 pFN21AE2073 6913 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4723 278 Size: 53 83 186 936 3187 2468 Fragments arranged by size: 3187 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2723 4256 1937 Size: 786 1533 4594 Fragments arranged by size: 4594 1533 786 Acc65I G'GTAC_C Cuts at: 4234 4234 Size: 6913 AclI AA'CG_TT Cuts at: 1563 5040 5413 1563 Size: 3477 373 3063 Fragments arranged by size: 3477 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2702 3851 4976 6360 6628 1632 Size: 1070 1149 1125 1384 268 1917 Fragments arranged by size: 1917 1384 1149 1125 1070 268 AflII C'TTAA_G Cuts at: 820 1017 4046 820 Size: 197 3029 3687 Fragments arranged by size: 3687 3029 197 AflIII A'CryG_T Cuts at: 1203 3451 5812 6789 1203 Size: 2248 2361 977 1327 Fragments arranged by size: 2361 2248 1327 977 AgeI A'CCGG_T Cuts at: 5744 5744 Size: 6913 AhdI GACnn_n'nnGTC Cuts at: 5642 5642 Size: 6913 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6913 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6913 AlwNI CAG_nnn'CTG Cuts at: 3644 4656 6228 3644 Size: 1012 1572 4329 Fragments arranged by size: 4329 1572 1012 ApaLI G'TGCA_C Cuts at: 2637 4970 6126 2637 Size: 2333 1156 3424 Fragments arranged by size: 3424 2333 1156 ApoI r'AATT_y Cuts at: 1493 2106 2291 3897 4207 4222 4448 4600 6709 Size: 613 185 1606 310 15 226 152 2109 Cuts at: 6709 1493 Size: 1697 Fragments arranged by size: 2109 1697 1606 613 310 226 185 152 15 AseI AT'TA_AT Cuts at: 160 1032 5467 160 Size: 872 4435 1606 Fragments arranged by size: 4435 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6913 AvaI C'yCGr_G Cuts at: 1870 1943 2079 4238 1870 Size: 73 136 2159 4545 Fragments arranged by size: 4545 2159 136 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2242 2275 2242 Size: 33 6880 BamHI G'GATC_C Cuts at: 3496 4243 4610 3496 Size: 747 367 5799 Fragments arranged by size: 5799 747 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2971 3784 4234 5689 618 Size: 325 217 627 1184 813 450 1455 1842 Fragments arranged by size: 1842 1455 1184 813 627 450 325 217 BanII G_rGCy'C Cuts at: 1391 2776 4232 1391 Size: 1385 1456 4072 Fragments arranged by size: 4072 1456 1385 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2850 2915 3579 928 Size: 971 951 65 664 4262 Fragments arranged by size: 4262 971 951 664 65 BbvCI CC'TCA_GC Cuts at: 3413 3516 3413 Size: 103 6810 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3437 3471 5104 5138 1903 Size: 34 1500 34 1633 34 3678 Fragments arranged by size: 3678 1633 1500 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4805 6015 4805 Size: 1210 5703 BclI T'GATC_A Cuts at: 1548 1548 Size: 6913 BfrBI ATG'CAT Cuts at: 2033 2987 3859 2033 Size: 954 872 5087 Fragments arranged by size: 5087 954 872 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5524 136 Size: 107 122 71 631 605 3852 1525 Fragments arranged by size: 3852 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2713 3173 6908 2713 Size: 460 3735 2718 Fragments arranged by size: 3735 2718 460 BlpI GC'TnA_GC Cuts at: 3651 4330 3651 Size: 679 6234 Bme1580I G_kGCm'C Cuts at: 1790 2641 2974 4974 6130 1790 Size: 851 333 2000 1156 2573 Fragments arranged by size: 2573 2000 1156 851 333 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6913 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2977 3223 4644 Size: 416 53 83 399 24 1560 246 1421 Cuts at: 4644 5602 442 Size: 958 1753 Fragments arranged by size: 1753 1560 1421 958 416 399 246 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2760 3114 5573 1239 Size: 1521 354 2459 2579 Fragments arranged by size: 2579 2459 1521 354 Bpu10I CC'TnA_GC Cuts at: 3413 3516 3413 Size: 103 6810 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2573 3351 5038 5903 6201 2573 Size: 778 1687 865 298 3285 Fragments arranged by size: 3285 1687 865 778 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4157 5576 882 Size: 617 2658 1419 2219 Fragments arranged by size: 2658 2219 1419 617 BsaAI yAC'GTr Cuts at: 493 1204 2218 4704 493 Size: 711 1014 2486 2702 Fragments arranged by size: 2702 2486 1014 711 BsaBI GATnn'nnATC Cuts at: 6907 6907 Size: 6913 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4720 5102 5737 275 Size: 53 83 186 936 3187 382 635 1451 Fragments arranged by size: 3187 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5346 5744 6018 6165 6576 5346 Size: 398 274 147 411 5683 Fragments arranged by size: 5683 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3108 3138 3993 4023 1178 Size: 30 165 30 1705 30 855 30 4068 Fragments arranged by size: 4068 1705 855 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2538 3066 3111 4001 1186 Size: 1352 528 45 890 4098 Fragments arranged by size: 4098 1352 890 528 45 BseYI C'CCAG_C Cuts at: 3891 3953 6116 6612 3891 Size: 62 2163 496 4192 Fragments arranged by size: 4192 2163 496 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3356 1163 Size: 573 1620 4720 Fragments arranged by size: 4720 1620 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4363 5124 6152 1540 Size: 275 186 2362 761 1028 2301 Fragments arranged by size: 2362 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2641 2776 4974 5059 6130 2641 Size: 135 2198 85 1071 3424 Fragments arranged by size: 3424 2198 1071 135 85 BsmI GAATG_Cn' Cuts at: 3360 4424 4517 3360 Size: 1064 93 5756 Fragments arranged by size: 5756 1064 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3365 3365 Size: 6913 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2641 2776 2974 4232 4974 5059 6130 Size: 399 851 135 198 1258 742 85 1071 Cuts at: 6130 1391 Size: 2174 Fragments arranged by size: 2174 1258 1071 851 742 399 198 135 85 BspEI T'CCGG_A Cuts at: 6576 6576 Size: 6913 BspHI T'CATG_A Cuts at: 3811 4802 6532 3811 Size: 991 1730 4192 Fragments arranged by size: 4192 1730 991 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4268 844 Size: 3424 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2557 4800 1394 Size: 429 734 2243 3507 Fragments arranged by size: 3507 2243 734 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5408 5582 65 Size: 1183 4160 174 1396 Fragments arranged by size: 4160 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5557 5744 1700 Size: 110 3747 187 2869 Fragments arranged by size: 3747 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6913 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6913 BssSI C'ACGA_G Cuts at: 4154 4973 5985 4154 Size: 819 1012 5082 Fragments arranged by size: 5082 1012 819 BstAPI GCAn_nnn'nTGC Cuts at: 794 2039 794 Size: 1245 5668 BstBI TT'CG_AA Cuts at: 3271 5717 3271 Size: 2446 4467 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6913 BstYI r'GATC_y Cuts at: 1972 2713 3173 3496 4098 4243 4610 4995 5012 Size: 741 460 323 602 145 367 385 17 Cuts at: 5012 6464 6550 6562 6908 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 741 602 460 385 367 346 323 145 86 17 12 BstZ17I GTA'TAC Cuts at: 2724 2724 Size: 6913 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5422 Fragments arranged by size: 5422 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6174 Fragments arranged by size: 6174 683 56 BtsI GCAGTG_nn' Cuts at: 2839 4429 5223 5243 2839 Size: 1590 794 20 4509 Fragments arranged by size: 4509 1590 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6913 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6878 DraI TTT'AAA Cuts at: 3057 4217 3057 Size: 1160 5753 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5920 809 Size: 507 4604 1802 Fragments arranged by size: 4604 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3464 4360 5249 Size: 54 1425 50 275 18 1634 896 889 Cuts at: 5249 6878 8 Size: 1629 43 Fragments arranged by size: 1634 1629 1425 896 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4360 1537 Size: 275 2548 4090 Fragments arranged by size: 4090 2548 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3084 3526 4843 1353 Size: 1731 442 1317 3423 Fragments arranged by size: 3423 1731 1317 442 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5500 5874 6020 387 Size: 1064 138 3911 374 146 1280 Fragments arranged by size: 3911 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2774 2774 Size: 6913 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2702 2760 3114 3851 4976 5573 6360 Size: 393 1070 58 354 737 1125 597 787 Cuts at: 6360 6628 1239 Size: 268 1524 Fragments arranged by size: 1524 1125 1070 787 737 597 393 354 268 58 EcoRI G'AATT_C Cuts at: 4222 4600 4222 Size: 378 6535 FspI TGC'GCA Cuts at: 5419 5419 Size: 6913 HaeII r_GCGC'y Cuts at: 1678 2892 6060 1678 Size: 1214 3168 2531 Fragments arranged by size: 3168 2531 1214 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2928 2960 3993 4025 5554 Size: 32 135 32 1356 32 1033 32 1529 Cuts at: 5554 5586 5628 5660 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1529 1356 1033 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2947 4257 4503 669 Size: 988 281 1009 1310 246 3079 Fragments arranged by size: 3079 1310 1009 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6913 HpaI GTT'AAC Cuts at: 4503 4503 Size: 6913 KpnI G_GTAC'C Cuts at: 4238 4238 Size: 6913 MfeI C'AATT_G Cuts at: 4070 4512 4070 Size: 442 6471 MluI A'CGCG_T Cuts at: 6789 6789 Size: 6913 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2563 6027 6211 1305 Size: 1258 3464 184 2007 Fragments arranged by size: 3464 2007 1258 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3466 10 Size: 54 1425 343 1634 3457 Fragments arranged by size: 3457 1634 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3456 3974 4871 5230 5389 518 Size: 648 2290 518 897 359 159 2042 Fragments arranged by size: 2290 2042 897 648 518 359 159 MspA1I CmG'CkG Cuts at: 1606 4329 5006 6154 6399 1606 Size: 2723 677 1148 245 2120 Fragments arranged by size: 2723 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5422 Fragments arranged by size: 5422 939 552 NdeI CA'TA_TG Cuts at: 387 2035 2101 2587 2625 3259 387 Size: 1648 66 486 38 634 4041 Fragments arranged by size: 4041 1648 634 486 66 38 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6913 NotI GC'GGCC_GC Cuts at: 4360 4360 Size: 6913 NsiI A_TGCA'T Cuts at: 2035 2989 3861 2035 Size: 954 872 5087 Fragments arranged by size: 5087 954 872 NspI r_CATG'y Cuts at: 1132 1229 2991 3107 3455 3877 4271 5816 1132 Size: 97 1762 116 348 422 394 1545 2229 Fragments arranged by size: 2229 1762 1545 422 394 348 116 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6913 PciI A'CATG_T Cuts at: 3451 5812 3451 Size: 2361 4552 PflMI CCAn_nnn'nTGG Cuts at: 1108 2762 4108 1108 Size: 1654 1346 3913 Fragments arranged by size: 3913 1654 1346 PmeI GTTT'AAAC Cuts at: 4217 4217 Size: 6913 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4982 5014 6495 6527 4982 Size: 32 1481 32 5368 Fragments arranged by size: 5368 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6913 PsiI TTA'TAA Cuts at: 4483 4483 Size: 6913 PstI C_TGCA'G Cuts at: 830 4265 830 Size: 3435 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6913 SacI G_AGCT'C Cuts at: 2776 2776 Size: 6913 SalI G'TCGA_C Cuts at: 1936 4255 1936 Size: 2319 4594 SbfI CC_TGCA'GG Cuts at: 4265 4265 Size: 6913 ScaI AGT'ACT Cuts at: 2443 5161 6873 2443 Size: 2718 1712 2483 Fragments arranged by size: 2718 2483 1712 SfcI C'TryA_G Cuts at: 826 1045 2428 2632 4261 5396 6077 6268 826 Size: 219 1383 204 1629 1135 681 191 1471 Fragments arranged by size: 1629 1471 1383 1135 681 219 204 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6913 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6913 SmaI CCC'GGG Cuts at: 1872 4240 1872 Size: 2368 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2552 3366 4046 5017 5918 6180 Size: 197 926 609 814 680 971 901 262 Cuts at: 6180 820 Size: 1553 Fragments arranged by size: 1553 971 926 901 814 680 609 262 197 SnaBI TAC'GTA Cuts at: 493 2218 4704 493 Size: 1725 2486 2702 Fragments arranged by size: 2702 2486 1725 SpeI A'CTAG_T Cuts at: 152 152 Size: 6913 SphI G_CATG'C Cuts at: 1132 2991 4271 1132 Size: 1859 1280 3774 Fragments arranged by size: 3774 1859 1280 SspI AAT'ATT Cuts at: 5 52 3187 3383 4837 5 Size: 47 3135 196 1454 2081 Fragments arranged by size: 3135 2081 1454 196 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6913 StyI C'CwwG_G Cuts at: 513 1065 2004 2481 3549 4352 513 Size: 552 939 477 1068 803 3074 Fragments arranged by size: 3074 1068 939 803 552 477 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2989 3150 4940 4957 5110 5295 5740 Size: 1255 1409 161 1790 17 153 185 445 Cuts at: 5740 325 Size: 1498 Fragments arranged by size: 1790 1498 1409 1255 445 185 161 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2278 2441 3115 Size: 274 80 33 51 1443 301 163 674 Cuts at: 3115 3830 4132 5159 6871 96 Size: 715 302 1027 1712 138 Fragments arranged by size: 1712 1443 1027 715 674 302 301 274 163 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2843 2875 6873 6905 2843 Size: 32 3998 32 2851 Fragments arranged by size: 3998 2851 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6913 XbaI T'CTAG_A Cuts at: 4249 4249 Size: 6913 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6913 XmaI C'CCGG_G Cuts at: 1870 4238 1870 Size: 2368 4545 XmnI GAAnn'nnTTC Cuts at: 3267 5042 3267 Size: 1775 5138 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4721 276 Size: 53 83 186 936 3187 2468 Fragments arranged by size: 3187 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BstXI Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI