(Circular) (Six-base) MAPSORT of: pFN21AE2598.seq Check: 5039 from: 1 to: 5866 pFN21AE2598 5866 bp With 182 enzymes: SgfI * January 12, 2016 11:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3676 278 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3209 1937 Size: 1272 4594 Acc65I G'GTAC_C Cuts at: 3187 3187 Size: 5866 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2792 3929 5313 5581 1632 Size: 1160 1137 1384 268 1917 Fragments arranged by size: 1917 1384 1160 1137 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5669 AflIII A'CryG_T Cuts at: 1203 4765 5742 1203 Size: 3562 977 1327 Fragments arranged by size: 3562 1327 977 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 4595 4595 Size: 5866 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5866 AlwNI CAG_nnn'CTG Cuts at: 2300 2606 2987 3609 5181 2300 Size: 306 381 622 1572 2985 Fragments arranged by size: 2985 1572 622 381 306 ApaLI G'TGCA_C Cuts at: 2238 3923 5079 2238 Size: 1685 1156 3025 Fragments arranged by size: 3025 1685 1156 ApoI r'AATT_y Cuts at: 1493 2044 2349 2392 2660 3175 3401 3553 5662 Size: 551 305 43 268 515 226 152 2109 Cuts at: 5662 1493 Size: 1697 Fragments arranged by size: 2109 1697 551 515 305 268 226 152 43 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 3191 1870 Size: 73 1248 4545 Fragments arranged by size: 4545 1248 73 BamHI G'GATC_C Cuts at: 2081 3196 3563 2081 Size: 1115 367 4384 Fragments arranged by size: 4384 1115 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3187 4642 618 Size: 325 217 627 1400 1455 1842 Fragments arranged by size: 1842 1455 1400 627 325 217 BanII G_rGCy'C Cuts at: 1391 3185 1391 Size: 1794 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3102 928 Size: 971 1203 3692 Fragments arranged by size: 3692 1203 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 1903 Size: 34 2120 34 3678 Fragments arranged by size: 3678 2120 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2199 3758 4968 2199 Size: 1559 1210 3097 Fragments arranged by size: 3097 1559 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5866 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4477 136 Size: 107 122 71 631 605 2805 1525 Fragments arranged by size: 2805 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5861 5861 Size: 5866 BlpI GC'TnA_GC Cuts at: 3283 3283 Size: 5866 Bme1580I G_kGCm'C Cuts at: 1790 2242 3927 5083 1790 Size: 452 1685 1156 2573 Fragments arranged by size: 2573 1685 1156 452 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5866 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2275 3597 4555 Size: 416 53 83 399 24 858 1322 958 Cuts at: 4555 442 Size: 1753 Fragments arranged by size: 1753 1322 958 858 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2232 2876 3009 4526 1239 Size: 993 644 133 1517 2579 Fragments arranged by size: 2579 1517 993 644 133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2346 2651 3991 4856 5154 2346 Size: 305 1340 865 298 3058 Fragments arranged by size: 3058 1340 865 305 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4529 882 Size: 617 3030 2219 Fragments arranged by size: 3030 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3657 493 Size: 711 2453 2702 Fragments arranged by size: 2702 2453 711 BsaBI GATnn'nnATC Cuts at: 5860 5860 Size: 5866 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3673 4055 4690 275 Size: 53 83 186 936 2140 382 635 1451 Fragments arranged by size: 2140 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2084 4299 4697 4971 5118 5529 2084 Size: 2215 398 274 147 411 2421 Fragments arranged by size: 2421 2215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5641 Fragments arranged by size: 5641 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5866 BseYI C'CCAG_C Cuts at: 5069 5565 5069 Size: 496 5370 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2805 1163 Size: 573 1069 4224 Fragments arranged by size: 4224 1069 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 4077 5105 1540 Size: 275 186 1315 761 1028 2301 Fragments arranged by size: 2301 1315 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2242 2635 3927 4012 5083 2242 Size: 393 1292 85 1071 3025 Fragments arranged by size: 3025 1292 1071 393 85 BsmI GAATG_Cn' Cuts at: 3377 3470 3377 Size: 93 5773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2242 2635 3185 3927 4012 5083 1391 Size: 399 452 393 550 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 550 452 399 393 85 BspEI T'CCGG_A Cuts at: 5529 5529 Size: 5866 BspHI T'CATG_A Cuts at: 3755 5485 3755 Size: 1730 4136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2530 2827 3221 844 Size: 1686 297 394 3489 Fragments arranged by size: 3489 1686 394 297 BsrBI CCG'CTC Cuts at: 1394 1823 3753 1394 Size: 429 1930 3507 Fragments arranged by size: 3507 1930 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2011 4361 4535 65 Size: 1183 763 2350 174 1396 Fragments arranged by size: 2350 1396 1183 763 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4510 4697 1700 Size: 110 2700 187 2869 Fragments arranged by size: 2869 2700 187 110 BsrGI T'GTAC_A Cuts at: 96 2577 2793 96 Size: 2481 216 3169 Fragments arranged by size: 3169 2481 216 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5866 BssSI C'ACGA_G Cuts at: 3926 4938 3926 Size: 1012 4854 BstAPI GCAn_nnn'nTGC Cuts at: 794 2522 794 Size: 1728 4138 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5866 BstYI r'GATC_y Cuts at: 1972 2081 3196 3563 3948 3965 5417 5503 5515 Size: 109 1115 367 385 17 1452 86 12 Cuts at: 5515 5861 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1115 385 367 346 109 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4375 Fragments arranged by size: 4375 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5127 Fragments arranged by size: 5127 683 56 BtsI GCAGTG_nn' Cuts at: 3382 4176 4196 3382 Size: 794 20 5052 Fragments arranged by size: 5052 794 20 ClaI AT'CG_AT Cuts at: 1554 2052 1554 Size: 498 5368 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 2169 3170 2169 Size: 1001 4865 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4873 809 Size: 507 3557 1802 Fragments arranged by size: 3557 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3313 4202 5831 Size: 54 1425 50 275 18 1483 889 1629 Cuts at: 5831 8 Size: 43 Fragments arranged by size: 1629 1483 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3313 1537 Size: 275 1501 4090 Fragments arranged by size: 4090 1501 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 3796 1353 Size: 719 1724 3423 Fragments arranged by size: 3423 1724 719 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4453 4827 4973 387 Size: 1064 138 2864 374 146 1280 Fragments arranged by size: 2864 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2792 2876 3009 3929 4526 5313 Size: 393 600 560 84 133 920 597 787 Cuts at: 5313 5581 1239 Size: 268 1524 Fragments arranged by size: 1524 920 787 600 597 560 393 268 133 84 EcoNI CCTnn'n_nnAGG Cuts at: 2106 2106 Size: 5866 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 EcoRV GAT'ATC Cuts at: 2598 2598 Size: 5866 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2387 2822 2387 Size: 435 5431 FspI TGC'GCA Cuts at: 4372 4372 Size: 5866 HaeII r_GCGC'y Cuts at: 1678 5013 1678 Size: 3335 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2982 3014 3092 3110 3124 Size: 32 135 32 1410 32 78 18 14 Cuts at: 3124 3142 4507 4539 4581 4613 1373 Size: 18 1365 32 42 32 2626 Fragments arranged by size: 2626 1410 1365 135 78 42 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 3210 3456 669 Size: 988 281 1272 246 3079 Fragments arranged by size: 3079 1272 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5866 HpaI GTT'AAC Cuts at: 3456 3456 Size: 5866 KpnI G_GTAC'C Cuts at: 3191 3191 Size: 5866 MfeI C'AATT_G Cuts at: 2526 3465 2526 Size: 939 4927 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2766 2983 4980 5164 1305 Size: 1461 217 1997 184 2007 Fragments arranged by size: 2007 1997 1461 217 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4044 Fragments arranged by size: 4044 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3824 4183 4342 518 Size: 648 2658 359 159 2042 Fragments arranged by size: 2658 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3282 3959 5107 5352 1606 Size: 1676 677 1148 245 2120 Fragments arranged by size: 2120 1676 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4375 Fragments arranged by size: 4375 939 552 NdeI CA'TA_TG Cuts at: 387 2637 387 Size: 2250 3616 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NspI r_CATG'y Cuts at: 1132 1229 3137 3224 4769 1132 Size: 97 1908 87 1545 2229 Fragments arranged by size: 2229 1908 1545 97 87 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5866 PciI A'CATG_T Cuts at: 4765 4765 Size: 5866 PflMI CCAn_nnn'nTGG Cuts at: 1108 2480 1108 Size: 1372 4494 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 5448 5480 3935 Size: 32 1481 32 4321 Fragments arranged by size: 4321 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5866 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PstI C_TGCA'G Cuts at: 830 2983 3218 830 Size: 2153 235 3478 Fragments arranged by size: 3478 2153 235 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 SalI G'TCGA_C Cuts at: 1936 3208 1936 Size: 1272 4594 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 4114 5826 4114 Size: 1712 4154 SfcI C'TryA_G Cuts at: 826 1045 2949 2979 3214 4349 5030 5221 826 Size: 219 1904 30 235 1135 681 191 1471 Fragments arranged by size: 1904 1471 1135 681 235 219 191 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5866 SmaI CCC'GGG Cuts at: 1872 3193 1872 Size: 1321 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2361 2666 3970 4871 5133 820 Size: 197 926 418 305 1304 901 262 1553 Fragments arranged by size: 1553 1304 926 901 418 305 262 197 SnaBI TAC'GTA Cuts at: 493 3657 493 Size: 3164 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 3137 3224 1132 Size: 2005 87 3774 Fragments arranged by size: 3774 2005 87 SspI AAT'ATT Cuts at: 5 52 3790 5 Size: 47 3738 2081 Fragments arranged by size: 3738 2081 47 StuI AGG'CCT Cuts at: 1517 2460 1517 Size: 943 4923 StyI C'CwwG_G Cuts at: 513 1065 2004 3305 513 Size: 552 939 1301 3074 Fragments arranged by size: 3074 1301 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3893 3910 4063 4248 4693 325 Size: 1255 2313 17 153 185 445 1498 Fragments arranged by size: 2313 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2577 2793 4112 Size: 274 80 33 51 1443 600 216 1319 Cuts at: 4112 5824 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1319 600 274 216 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5826 5858 5826 Size: 32 5834 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5866 XbaI T'CTAG_A Cuts at: 3202 3202 Size: 5866 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5866 XmaI C'CCGG_G Cuts at: 1870 3191 1870 Size: 1321 4545 XmnI GAAnn'nnTTC Cuts at: 3995 3995 Size: 5866 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3674 276 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI