(Linear) (Six-base) MAPSORT of: KIEE2598.seq  Check: 7006  from: 1  to: 1161



KIEE2598.orf

 With 182 enzymes: SgfI * 

                        January 12, 2016 10:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     787    1161 
   Size:        787     374
 
AlwNI CAG_nnn'CTG

Cuts at:      0     295     601     982    1161 
   Size:        295     306     381     179

  Fragments arranged by size: 

                 381     306     295     179
 
ApaLI G'TGCA_C

Cuts at:      0     233    1161 
   Size:        233     928
 
ApoI r'AATT_y

Cuts at:      0      39     344     387     655    1161 
   Size:         39     305      43     268     506

  Fragments arranged by size: 

                 506     305     268      43      39
 
BamHI G'GATC_C

Cuts at:      0      76    1161 
   Size:         76    1085
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1097    1161 
   Size:       1097      64
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     194    1161 
   Size:        194     967
 
Bme1580I G_kGCm'C

Cuts at:      0     237    1161 
   Size:        237     924
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     270    1161 
   Size:        270     891
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     871    1004    1161 
   Size:        227     644     133     157

  Fragments arranged by size: 

                 644     227     157     133
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     646    1161 
   Size:        341     305     515

  Fragments arranged by size: 

                 515     341     305
 
BsaWI w'CCGG_w

Cuts at:      0      79    1161 
   Size:         79    1082
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     800    1161 
   Size:        800     361
 
BsiHKAI G_wGCw'C

Cuts at:      0     237     630    1161 
   Size:        237     393     531

  Fragments arranged by size: 

                 531     393     237
 
Bsp1286I G_dGCh'C

Cuts at:      0     237     630    1161 
   Size:        237     393     531

  Fragments arranged by size: 

                 531     393     237
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     525     822    1161 
   Size:        525     297     339

  Fragments arranged by size: 

                 525     339     297
 
BsrDI GCAATG_nn'

Cuts at:      0       6    1161 
   Size:          6    1155
 
BsrGI T'GTAC_A

Cuts at:      0     572     788    1161 
   Size:        572     216     373

  Fragments arranged by size: 

                 572     373     216
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     517    1161 
   Size:        517     644
 
BstYI r'GATC_y

Cuts at:      0      76    1161 
   Size:         76    1085
 
ClaI AT'CG_AT

Cuts at:      0      47    1161 
   Size:         47    1114
 
DraI TTT'AAA

Cuts at:      0     164    1161 
   Size:        164     997
 
EarI CTCTTCn'nnn_

Cuts at:      0      67    1161 
   Size:         67    1094
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     787     871    1004    1161 
   Size:        227     560      84     133     157

  Fragments arranged by size: 

                 560     227     157     133      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     101    1161 
   Size:        101    1060
 
EcoRV GAT'ATC

Cuts at:      0     593    1161 
   Size:        593     568
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     382     817    1161 
   Size:        382     435     344

  Fragments arranged by size: 

                 435     382     344
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     977    1009    1087    1105    1119    1137    1161 
   Size:        977      32      78      18      14      18      24

  Fragments arranged by size: 

                 977      78      32      24      18      18      14
 
MfeI C'AATT_G

Cuts at:      0     521    1161 
   Size:        521     640
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     761     978    1161 
   Size:        761     217     183

  Fragments arranged by size: 

                 761     217     183
 
NdeI CA'TA_TG

Cuts at:      0     632    1161 
   Size:        632     529
 
NspI r_CATG'y

Cuts at:      0    1132    1161 
   Size:       1132      29
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     475    1161 
   Size:        475     686
 
PstI C_TGCA'G

Cuts at:      0     978    1161 
   Size:        978     183
 
SfcI C'TryA_G

Cuts at:      0     944     974    1161 
   Size:        944      30     187

  Fragments arranged by size: 

                 944     187      30
 
SmlI C'TyrA_G

Cuts at:      0     356     661    1161 
   Size:        356     305     500

  Fragments arranged by size: 

                 500     356     305
 
SphI G_CATG'C

Cuts at:      0    1132    1161 
   Size:       1132      29
 
StuI AGG'CCT

Cuts at:      0     455    1161 
   Size:        455     706
 
TatI w'GTAC_w

Cuts at:      0     572     788    1161 
   Size:        572     216     373

  Fragments arranged by size: 

                 572     373     216
 

 Enzymes that do cut:

     AcuI    AlwNI    ApaLI     ApoI    BamHI     BbsI    BciVI Bme1580I
     BmrI     BpmI    BpuEI    BsaWI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrDI    BsrGI   BstAPI    BstYI     ClaI     DraI     EarI  Eco57MI
    EcoNI    EcoRV     FalI    Hin4I     MfeI     MmeI     NdeI     NspI
    PflMI     PstI     SfcI     SmlI     SphI     StuI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI EcoO109I    EcoRI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI