################################## # NO CUT ENZYMES ( 203 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MboI MboII MflI MluI MluNI MroI MscI MseI MslI MspI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I TspRI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AciI 177 GCGG CCGC(-3/-1) AcsI 151 GAATTC [GA]^AATT[CT] AfaI 218 GTAC GT^AC AflIII 70 ACACGT A^C[GA][CT]GT ApoI 151 GAATTC [GA]^AATT[CT] AspHI 28 GTGCTC G[AT]GC[AT]^C AvaII 131 GGACC G^G[AT]CC BbrPI 71 CACGTG CAC^GTG BmyI 28 GTGCTC G[AGT]GC[ACT]^C BsaAI 71 CACGTG [CT]AC^GT[GA] BsiHKAI 28 GTGCTC G[AT]GC[AT]^C BsmAI 50 GTCTC GTCTC(1/5) Bsp1286I 28 GTGCTC G[AGT]GC[ACT]^C BspMI 94 ACCTGC ACCTGC(4/8) BsrI 158 CCAGT ACTGG(1/-1) BstF5I 223 CATCC GGATG(2/0) Cac8I 177 GCGGGC GC[ACGT]^[ACGT]GC DdeI 46 CTGAG C^T[ACGT]AG EcoRI 151 GAATTC G^AATTC FauI 177 GCGGG CCCGC(4/6) FokI 223 CATCC GGATG(9/13) HinfI 48 GAGTC G^A[ACGT]TC HphI 155 TCACC GGTGA(8/7) MaeII 72 ACGT A^CGT MfeI 277 CAATTG C^AATTG MunI 277 CAATTG C^AATTG PleI 48 GAGTC GAGTC(4/5) PmaCI 71 CACGTG CAC^GTG PmlI 71 CACGTG CAC^GTG PvuII 165 CAGCTG CAG^CTG RsaI 218 GTAC GT^AC Tsp45I 80 GTGAC ^GT[GC]AC XcmI 226 CCAAGGAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AluI 145 AGCT AG^CT 166 AGCT BbvI 98 GCTGC GCAGC(8/12) 167 GCTGC Cfr13I 6 GGGCC G^G[ACGT]CC 131 GGACC Fnu4HI 98 GCTGC GC^[ACGT]GC 167 GCTGC ItaI 98 GCTGC GC^[ACGT]GC 167 GCTGC MaeIII 80 GTGAC ^GT[ACGT]AC 161 GTAAC MspA1I 165 CAGCTG C[AC]G^C[GT]G 175 CAGCGG Sau96I 6 GGGCC G^G[ACGT]CC 131 GGACC TseI 98 GCTGC G^C[AT]GC 167 GCTGC Tsp509I 152 AATT ^AATT 278 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### BsaJI BsiYI BslI BssKI BstNI EcoRII EcoT14I HaeIII MnlI MvaI ScrFI StyI