################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 281 GGTACC G^GTACC AccII 607 CGCG CG^CG AfaI 282 GTAC GT^AC Aor51HI 36 AGCGCT AGC^GCT Asp718I 281 GGTACC G^GTACC AvaI 6 CTCGGG C^[CT]CG[GA]G BalI 193 TGGCCA TGG^CCA BamHI 264 GGATCC G^GATCC BanII 3 GAGCTC G[GA]GC[CT]^C BbsI 510 GAAGAC GAAGAC(2/6) BbvCI 489 CCTCAGC CCTCAGC(-5/-2) BpuAI 510 GAAGAC GAAGAC(2/6) BsaAI 697 TACGTG [CT]AC^GT[GA] BsaI 529 GGTCTC GGTCTC(1/5) BsaWI 690 ACCGGA [AT]^CCGG[AT] BseRI 676 GAGGAG GAGGAG(10/8) BsoBI 6 CTCGGG C^[CT]CG[GA]G BsrDI 339 CATTGC GCAATG(2/0) BstUI 607 CGCG CG^CG BstXI 338 CCATTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 264 GGATCC [GA]^GATC[CT] DpnII 265 GATC ^GATC DsaI 344 CCATGG C^C[GA][CT]GG EaeI 193 TGGCCA [CT]^GGCC[GA] Eco47III 36 AGCGCT AGC^GCT HaeII 36 AGCGCT [GA]GCGC^[CT] KpnI 281 GGTACC GGTAC^C MaeII 698 ACGT A^CGT MaeIII 498 GTCAC ^GT[ACGT]AC MboI 265 GATC ^GATC MflI 264 GGATCC [GA]^GATC[CT] MluNI 193 TGGCCA TGG^CCA MscI 193 TGGCCA TGG^CCA MslI 339 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 607 CGCG CG^CG NcoI 344 CCATGG C^CATGG NdeII 265 GATC ^GATC PleI 610 GAGTC GAGTC(4/5) PshAI 308 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 477 CTGCAG CTGCA^G RsaI 282 GTAC GT^AC SacI 3 GAGCTC GAGCT^C Sau3AI 265 GATC ^GATC SfcI 477 CTGCAG C^T[GA][CT]AG TaqI 373 TCGA T^CGA TfiI 565 GAATC G^A[AT]TC Tsp45I 498 GTCAC ^GT[GC]AC TthHB8I 373 TCGA T^CGA XcmI 614 CCAGGAAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 264 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AciI 208 GCGG CCGC(-3/-1) 366 GCGG AlwI 265 GATCC GGATC(4/5) 264 GGATC AspHI 3 GAGCTC G[AT]GC[AT]^C 415 GTGCTC BpmI 634 CTCCAG CTGGAG(16/14) 259 CTGGAG BsiHKAI 3 GAGCTC G[AT]GC[AT]^C 415 GTGCTC BsmAI 442 GAGAC GTCTC(1/5) 530 GTCTC Cac8I 186 GCAGGC GC[ACGT]^[ACGT]GC 425 GCAAGC EcoT14I 176 CCTTGG C^C[AT][AT]GG 344 CCATGG HinfI 565 GAATC G^A[ACGT]TC 610 GAGTC StyI 176 CCTTGG C^C[AT][AT]GG 344 CCATGG Tsp509I 245 AATT ^AATT 400 AATT TspRI 500 CACTG CA[GC]TG[ACGT][ACGT]^ 601 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AluI AvaII BanI BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV PpuMI Sau96I ScrFI SfaNI TseI