(Circular) (Six-base) MAPSORT of: pFN21AE1737.seq Check: 8121 from: 1 to: 5434 pFN21AE1737 5434 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3244 278 Size: 53 83 186 936 1708 2468 Fragments arranged by size: 2468 1708 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2777 1937 Size: 840 4594 Acc65I G'GTAC_C Cuts at: 2286 2755 2286 Size: 469 4965 AclI AA'CG_TT Cuts at: 1563 3561 3934 1563 Size: 1998 373 3063 Fragments arranged by size: 3063 1998 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2281 2534 3497 4881 5149 1632 Size: 649 253 963 1384 268 1917 Fragments arranged by size: 1917 1384 963 649 268 253 AfeI AGC'GCT Cuts at: 2043 2043 Size: 5434 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5237 AflIII A'CryG_T Cuts at: 1203 4333 5310 1203 Size: 3130 977 1327 Fragments arranged by size: 3130 1327 977 AgeI A'CCGG_T Cuts at: 4265 4265 Size: 5434 AhdI GACnn_n'nnGTC Cuts at: 4163 4163 Size: 5434 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5434 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5434 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2579 2611 2579 Size: 32 5402 AlwNI CAG_nnn'CTG Cuts at: 3177 4749 3177 Size: 1572 3862 ApaLI G'TGCA_C Cuts at: 3491 4647 3491 Size: 1156 4278 ApoI r'AATT_y Cuts at: 1493 2743 2969 3121 5230 1493 Size: 1250 226 152 2109 1697 Fragments arranged by size: 2109 1697 1250 226 152 AseI AT'TA_AT Cuts at: 160 1032 3988 160 Size: 872 2956 1606 Fragments arranged by size: 2956 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 AvaI C'yCGr_G Cuts at: 1870 1943 2011 2759 1870 Size: 73 68 748 4545 Fragments arranged by size: 4545 748 73 68 BamHI G'GATC_C Cuts at: 2269 2764 3131 2269 Size: 495 367 4572 Fragments arranged by size: 4572 495 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2221 2286 2453 2755 4210 Size: 325 217 627 434 65 167 302 1455 Cuts at: 4210 618 Size: 1842 Fragments arranged by size: 1842 1455 627 434 325 302 217 167 65 BanII G_rGCy'C Cuts at: 1391 2012 2753 1391 Size: 621 741 4072 Fragments arranged by size: 4072 741 621 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2522 928 Size: 971 623 3840 Fragments arranged by size: 3840 971 623 BbvCI CC'TCA_GC Cuts at: 2495 2495 Size: 5434 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3625 3659 1903 Size: 34 1688 34 3678 Fragments arranged by size: 3678 1688 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3326 4536 3326 Size: 1210 4224 BclI T'GATC_A Cuts at: 1548 1548 Size: 5434 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4045 136 Size: 107 122 71 631 605 2373 1525 Fragments arranged by size: 2373 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5429 5429 Size: 5434 BlpI GC'TnA_GC Cuts at: 2851 2851 Size: 5434 Bme1580I G_kGCm'C Cuts at: 1790 2458 3495 4651 1790 Size: 668 1037 1156 2573 Fragments arranged by size: 2573 1156 1037 668 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5434 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2374 3165 4123 Size: 416 53 83 399 24 957 791 958 Cuts at: 4123 442 Size: 1753 Fragments arranged by size: 1753 958 957 791 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5434 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2510 2510 Size: 5434 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 2624 4094 1239 Size: 1046 339 1470 2579 Fragments arranged by size: 2579 1470 1046 339 Bpu10I CC'TnA_GC Cuts at: 2495 2495 Size: 5434 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3559 4424 4722 3559 Size: 865 298 4271 Fragments arranged by size: 4271 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2540 4097 882 Size: 617 1041 1557 2219 Fragments arranged by size: 2219 1557 1041 617 BsaAI yAC'GTr Cuts at: 493 1204 2704 3225 493 Size: 711 1500 521 2702 Fragments arranged by size: 2702 1500 711 521 BsaBI GATnn'nnATC Cuts at: 5428 5428 Size: 5434 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3241 3623 4258 275 Size: 53 83 186 936 1708 382 635 1451 Fragments arranged by size: 1708 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2695 3867 4265 4539 4686 5097 2695 Size: 1172 398 274 147 411 3032 Fragments arranged by size: 3032 1172 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5209 Fragments arranged by size: 5209 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2696 1186 Size: 1510 3924 BseYI C'CCAG_C Cuts at: 2258 2457 4637 5133 2258 Size: 199 2180 496 2559 Fragments arranged by size: 2559 2180 496 199 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4861 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2884 3645 4673 1540 Size: 275 186 883 761 1028 2301 Fragments arranged by size: 2301 1028 883 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2424 3495 3580 4651 2012 Size: 412 1071 85 1071 2795 Fragments arranged by size: 2795 1071 1071 412 85 BsmI GAATG_Cn' Cuts at: 2945 3038 2945 Size: 93 5341 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2424 2458 2753 3495 3580 4651 Size: 399 222 412 34 295 742 85 1071 Cuts at: 4651 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 412 399 295 222 85 34 BspEI T'CCGG_A Cuts at: 5097 5097 Size: 5434 BspHI T'CATG_A Cuts at: 3323 5053 3323 Size: 1730 3704 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2789 844 Size: 1945 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3321 1394 Size: 429 1498 3507 Fragments arranged by size: 3507 1498 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2343 3929 4103 65 Size: 1183 1095 1586 174 1396 Fragments arranged by size: 1586 1396 1183 1095 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4078 4265 1700 Size: 110 2268 187 2869 Fragments arranged by size: 2869 2268 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5434 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5434 BssSI C'ACGA_G Cuts at: 3494 4506 3494 Size: 1012 4422 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5434 BstBI TT'CG_AA Cuts at: 4238 4238 Size: 5434 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5434 BstXI CCAn_nnnn'nTGG Cuts at: 2350 2350 Size: 5434 BstYI r'GATC_y Cuts at: 1972 2269 2764 3131 3516 3533 4985 5071 5083 Size: 297 495 367 385 17 1452 86 12 Cuts at: 5083 5429 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 495 385 367 346 297 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2349 513 Size: 552 1284 3598 Fragments arranged by size: 3598 1284 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4695 Fragments arranged by size: 4695 683 56 BtsI GCAGTG_nn' Cuts at: 2950 3744 3764 2950 Size: 794 20 4620 Fragments arranged by size: 4620 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5434 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5399 DraI TTT'AAA Cuts at: 2738 2738 Size: 5434 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4441 809 Size: 507 3125 1802 Fragments arranged by size: 3125 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2198 2881 3770 Size: 54 1425 50 275 18 368 683 889 Cuts at: 3770 5399 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 683 368 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2881 1537 Size: 275 1069 4090 Fragments arranged by size: 4090 1069 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3364 1353 Size: 2011 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2228 4021 4395 4541 387 Size: 1064 138 639 1793 374 146 1280 Fragments arranged by size: 1793 1280 1064 639 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 5434 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2281 2285 2534 2624 3497 4094 4881 Size: 393 649 4 249 90 873 597 787 Cuts at: 4881 5149 1239 Size: 268 1524 Fragments arranged by size: 1524 873 787 649 597 393 268 249 90 4 EcoO109I rG'GnC_Cy Cuts at: 2166 2204 2491 2166 Size: 38 287 5109 Fragments arranged by size: 5109 287 38 EcoRI G'AATT_C Cuts at: 2743 3121 2743 Size: 378 5056 FspI TGC'GCA Cuts at: 3940 3940 Size: 5434 HaeII r_GCGC'y Cuts at: 1678 2045 4581 1678 Size: 367 2536 2531 Fragments arranged by size: 2536 2531 367 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2261 2293 2555 2587 4075 Size: 32 135 32 689 32 262 32 1488 Cuts at: 4075 4107 4149 4181 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1488 689 262 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2778 3024 669 Size: 988 281 840 246 3079 Fragments arranged by size: 3079 988 840 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5434 HpaI GTT'AAC Cuts at: 3024 3024 Size: 5434 KpnI G_GTAC'C Cuts at: 2290 2759 2290 Size: 469 4965 MfeI C'AATT_G Cuts at: 3033 3033 Size: 5434 MluI A'CGCG_T Cuts at: 5310 5310 Size: 5434 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2301 4548 4732 1305 Size: 996 2247 184 2007 Fragments arranged by size: 2247 2007 996 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2200 10 Size: 54 1425 343 368 3244 Fragments arranged by size: 3244 1425 368 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 3392 3751 3910 518 Size: 648 1182 1044 359 159 2042 Fragments arranged by size: 2042 1182 1044 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2850 3527 4675 4920 1606 Size: 1244 677 1148 245 2120 Fragments arranged by size: 2120 1244 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2349 513 Size: 552 1284 3598 Fragments arranged by size: 3598 1284 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5434 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5434 NotI GC'GGCC_GC Cuts at: 2881 2881 Size: 5434 NspI r_CATG'y Cuts at: 1132 1229 2792 4337 1132 Size: 97 1563 1545 2229 Fragments arranged by size: 2229 1563 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5434 PciI A'CATG_T Cuts at: 4333 4333 Size: 5434 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5434 PfoI T'CCnGG_A Cuts at: 2168 2618 2168 Size: 450 4984 PmeI GTTT'AAAC Cuts at: 2738 2738 Size: 5434 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3503 3535 5016 5048 3503 Size: 32 1481 32 3889 Fragments arranged by size: 3889 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2166 2204 2491 2166 Size: 38 287 5109 Fragments arranged by size: 5109 287 38 PshAI GACnn'nnGTC Cuts at: 1613 2317 1613 Size: 704 4730 PsiI TTA'TAA Cuts at: 3004 3004 Size: 5434 PstI C_TGCA'G Cuts at: 830 2486 2786 830 Size: 1656 300 3478 Fragments arranged by size: 3478 1656 300 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5434 SacI G_AGCT'C Cuts at: 2012 2012 Size: 5434 SalI G'TCGA_C Cuts at: 1936 2776 1936 Size: 840 4594 SanDI GG'GwC_CC Cuts at: 2166 2166 Size: 5434 SbfI CC_TGCA'GG Cuts at: 2786 2786 Size: 5434 ScaI AGT'ACT Cuts at: 3682 5394 3682 Size: 1712 3722 SfcI C'TryA_G Cuts at: 826 1045 2482 2782 3917 4598 4789 826 Size: 219 1437 300 1135 681 191 1471 Fragments arranged by size: 1471 1437 1135 681 300 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5434 SmaI CCC'GGG Cuts at: 1872 2761 1872 Size: 889 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3538 4439 4701 820 Size: 197 926 1595 901 262 1553 Fragments arranged by size: 1595 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3225 493 Size: 2732 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5434 SphI G_CATG'C Cuts at: 1132 2792 1132 Size: 1660 3774 SspI AAT'ATT Cuts at: 5 52 3358 5 Size: 47 3306 2081 Fragments arranged by size: 3306 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5434 StyI C'CwwG_G Cuts at: 513 1065 2181 2349 2873 513 Size: 552 1116 168 524 3074 Fragments arranged by size: 3074 1116 552 524 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2239 3461 3478 3631 3816 4261 325 Size: 1255 659 1222 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1222 659 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3680 5392 96 Size: 274 80 33 51 1443 1703 1712 138 Fragments arranged by size: 1712 1703 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5394 5426 5394 Size: 32 5402 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5434 XbaI T'CTAG_A Cuts at: 2770 2770 Size: 5434 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2626 2626 Size: 5434 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5434 XmaI C'CCGG_G Cuts at: 1870 2759 1870 Size: 889 4545 XmnI GAAnn'nnTTC Cuts at: 3563 3563 Size: 5434 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3242 276 Size: 53 83 186 936 1708 2468 Fragments arranged by size: 2468 1708 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI