################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BanII BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BseRI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI MaeI MfeI MluI MluNI MscI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 565 GTAGAC GT^[AC][GT]AC AccII 10 CGCG CG^CG AccIII 21 TCCGGA T^CCGGA AcsI 595 AAATTC [GA]^AATT[CT] ApoI 595 AAATTC [GA]^AATT[CT] AvaI 470 CCCGAG C^[CT]CG[GA]G BamHI 466 GGATCC G^GATCC BanI 779 GGCGCC G^G[CT][GA]CC BbeI 779 GGCGCC GGCGC^C BclI 518 TGATCA T^GATCA BglI 532 GCCACAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 223 GGGCAC G[AGT]GC[ACT]^C BpmI 757 CTGGAG CTGGAG(16/14) BsaBI 96 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 293 GAGACC GGTCTC(1/5) BseAI 21 TCCGGA T^CCGGA BsgI 373 GTGCAG GTGCAG(16/14) BsmFI 6 GTCCC GGGAC(10/14) BsoBI 470 CCCGAG C^[CT]CG[GA]G Bsp1286I 223 GGGCAC G[AGT]GC[ACT]^C BspEI 21 TCCGGA T^CCGGA BsrBI 769 GAGCGG CCGCTC(-3/-3) BstUI 10 CGCG CG^CG BstYI 466 GGATCC [GA]^GATC[CT] DsaI 9 CCGCGG C^C[GA][CT]GG EarI 738 GAAGAG CTCTTC(1/4) FbaI 518 TGATCA T^GATCA KasI 779 GGCGCC G^GCGCC Ksp632I 738 GAAGAG CTCTTC(1/4) KspI 9 CCGCGG CCGC^GG MaeII 77 ACGT A^CGT MaeIII 164 GTTAC ^GT[ACGT]AC MamI 96 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 466 GGATCC [GA]^GATC[CT] MroI 21 TCCGGA T^CCGGA MseI 709 TTAA T^TAA MvnI 10 CGCG CG^CG NaeI 14 GCCGGC GCC^GGC NarI 779 GGCGCC GG^CGCC NgoMIV 14 GCCGGC G^CCGGC PstI 481 CTGCAG CTGCA^G PvuII 484 CAGCTG CAG^CTG SacII 9 CCGCGG CCGC^GG SfcI 481 CTGCAG C^T[GA][CT]AG SfoI 779 GGCGCC GGC^GCC Tru9I 709 TTAA T^TAA Tsp509I 596 AATT ^AATT XhoII 466 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AlwI 467 GATCC GGATC(4/5) 466 GGATC AvaII 34 GGACC G^G[AT]CC 276 GGACC BbvI 483 GCAGC GCAGC(8/12) 300 GCTGC BcnI 68 CCCGG CC^[GC]GG 462 CCGGG BsaWI 21 TCCGGA [AT]^CCGG[AT] 550 TCCGGT BsiEI 12 CGGCCG CG[GA][CT]^CG 819 CGGCCG BsmAI 58 GAGAC GTCTC(1/5) 293 GAGAC BspMI 167 ACCTGC ACCTGC(4/8) 296 ACCTGC BsrFI 14 GCCGGC [GA]^CCGG[CT] 817 ACCGGC BsrI 190 ACTGG ACTGG(1/-1) 810 CCAGT Cfr10I 14 GCCGGC [GA]^CCGG[CT] 817 ACCGGC DpnII 467 GATC ^GATC 519 GATC EaeI 12 CGGCCG [CT]^GGCC[GA] 819 CGGCCG EagI 12 CGGCCG C^GGCCG 819 CGGCCG EclXI 12 CGGCCG C^GGCCG 819 CGGCCG Eco52I 12 CGGCCG C^GGCCG 819 CGGCCG EcoRV 100 GATATC GAT^ATC 244 GATATC HaeII 419 AGCGCC [GA]GCGC^[CT] 779 GGCGCC HgaI 4 GCGTC GACGC(5/10) 18 GCGTC HinfI 679 GAGTC G^A[ACGT]TC 741 GAGTC HphI 732 GGTGA GGTGA(8/7) 689 TCACC MboI 467 GATC ^GATC 519 GATC MboII 738 GAAGA GAAGA(8/7) 762 GAAGA MspA1I 9 CCGCGG C[AC]G^C[GT]G 484 CAGCTG NciI 68 CCCGG CC^[GC]GG 462 CCGGG NdeII 467 GATC ^GATC 519 GATC PleI 679 GAGTC GAGTC(4/5) 741 GAGTC Sau3AI 467 GATC ^GATC 519 GATC SfaNI 323 GCATC GCATC(5/9) 356 GCATC TaqI 625 TCGA T^CGA 718 TCGA TseI 300 GCTGC G^C[AT]GC 483 GCAGC TthHB8I 625 TCGA T^CGA 718 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AcyI AfaI AluI BsaHI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MnlI MspI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI TspRI