(Circular) (Six-base) MAPSORT of: pFN21AE1774.seq Check: 1138 from: 1 to: 5548 pFN21AE1774 5548 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3358 278 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2571 2891 1937 Size: 634 320 4594 Fragments arranged by size: 4594 634 320 Acc65I G'GTAC_C Cuts at: 2869 2869 Size: 5548 AclI AA'CG_TT Cuts at: 1563 3675 4048 1563 Size: 2112 373 3063 Fragments arranged by size: 3063 2112 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3611 4995 5263 1632 Size: 1979 1384 268 1917 Fragments arranged by size: 1979 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5351 AflIII A'CryG_T Cuts at: 1203 4447 5424 1203 Size: 3244 977 1327 Fragments arranged by size: 3244 1327 977 AgeI A'CCGG_T Cuts at: 4379 4379 Size: 5548 AhdI GACnn_n'nnGTC Cuts at: 4277 4277 Size: 5548 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5548 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5548 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2675 2707 2675 Size: 32 5516 AlwNI CAG_nnn'CTG Cuts at: 3291 4863 3291 Size: 1572 3976 ApaLI G'TGCA_C Cuts at: 3605 4761 3605 Size: 1156 4392 ApoI r'AATT_y Cuts at: 1493 2600 2857 3083 3235 5344 1493 Size: 1107 257 226 152 2109 1697 Fragments arranged by size: 2109 1697 1107 257 226 152 AseI AT'TA_AT Cuts at: 160 1032 4102 160 Size: 872 3070 1606 Fragments arranged by size: 3070 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 AvaI C'yCGr_G Cuts at: 1870 1943 2475 2873 1870 Size: 73 532 398 4545 Fragments arranged by size: 4545 532 398 73 BamHI G'GATC_C Cuts at: 2471 2878 3245 2471 Size: 407 367 4774 Fragments arranged by size: 4774 407 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2784 2869 4324 618 Size: 325 217 627 997 85 1455 1842 Fragments arranged by size: 1842 1455 997 627 325 217 85 BanII G_rGCy'C Cuts at: 1391 2867 1391 Size: 1476 4072 BbeI G_GCGC'C Cuts at: 2788 2788 Size: 5548 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4577 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3739 3773 1903 Size: 34 1802 34 3678 Fragments arranged by size: 3678 1802 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3440 4650 3440 Size: 1210 4338 BclI T'GATC_A Cuts at: 1548 2523 1548 Size: 975 4573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2543 4159 136 Size: 107 122 71 631 605 871 1616 1525 Fragments arranged by size: 1616 1525 871 631 605 122 107 71 BglII A'GATC_T Cuts at: 5543 5543 Size: 5548 BlpI GC'TnA_GC Cuts at: 2965 2965 Size: 5548 Bme1580I G_kGCm'C Cuts at: 1790 2232 3609 4765 1790 Size: 442 1377 1156 2573 Fragments arranged by size: 2573 1377 1156 442 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5548 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2205 2809 3279 Size: 416 53 83 399 24 788 604 470 Cuts at: 3279 4237 442 Size: 958 1753 Fragments arranged by size: 1753 958 788 604 470 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2783 4208 1239 Size: 1544 1425 2579 Fragments arranged by size: 2579 1544 1425 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3673 4538 4836 3673 Size: 865 298 4385 Fragments arranged by size: 4385 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2292 4211 882 Size: 617 793 1919 2219 Fragments arranged by size: 2219 1919 793 617 BsaAI yAC'GTr Cuts at: 493 1204 3339 493 Size: 711 2135 2702 Fragments arranged by size: 2702 2135 711 BsaBI GATnn'nnATC Cuts at: 2105 5542 2105 Size: 3437 2111 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2023 2785 3355 Size: 53 83 186 936 476 14 762 570 Cuts at: 3355 3737 4372 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 762 635 570 476 382 186 83 53 14 BsaWI w'CCGG_w Cuts at: 2026 2555 3981 4379 4653 4800 5211 2026 Size: 529 1426 398 274 147 411 2363 Fragments arranged by size: 2363 1426 529 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2675 2705 1178 Size: 30 165 30 1272 30 4021 Fragments arranged by size: 4021 1272 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5548 BseYI C'CCAG_C Cuts at: 2123 2308 4751 5247 2123 Size: 185 2443 496 2424 Fragments arranged by size: 2443 2424 496 185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2399 1163 Size: 573 663 4312 Fragments arranged by size: 4312 663 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2020 2827 2998 3759 4787 1540 Size: 275 186 19 807 171 761 1028 2301 Fragments arranged by size: 2301 1028 807 761 275 186 171 19 BsiHKAI G_wGCw'C Cuts at: 3609 3694 4765 3609 Size: 85 1071 4392 Fragments arranged by size: 4392 1071 85 BsmI GAATG_Cn' Cuts at: 3059 3152 3059 Size: 93 5455 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2867 3609 3694 4765 1391 Size: 399 442 635 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 635 442 399 85 BspEI T'CCGG_A Cuts at: 2026 5211 2026 Size: 3185 2363 BspHI T'CATG_A Cuts at: 3437 5167 3437 Size: 1730 3818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2181 2310 2903 844 Size: 1337 129 593 3489 Fragments arranged by size: 3489 1337 593 129 BsrBI CCG'CTC Cuts at: 1394 1823 2776 3435 1394 Size: 429 953 659 3507 Fragments arranged by size: 3507 953 659 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4043 4217 65 Size: 1183 2795 174 1396 Fragments arranged by size: 2795 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2019 2822 4192 4379 1700 Size: 110 209 803 1370 187 2869 Fragments arranged by size: 2869 1370 803 209 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5548 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5548 BssSI C'ACGA_G Cuts at: 3608 4620 3608 Size: 1012 4536 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5548 BstBI TT'CG_AA Cuts at: 4352 4352 Size: 5548 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5548 BstYI r'GATC_y Cuts at: 1972 2471 2878 3245 3630 3647 5099 5185 5197 Size: 499 407 367 385 17 1452 86 12 Cuts at: 5197 5543 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 499 407 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2014 513 Size: 552 939 10 4047 Fragments arranged by size: 4047 939 552 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4809 Fragments arranged by size: 4809 683 56 BtsI GCAGTG_nn' Cuts at: 2387 3064 3858 3878 2387 Size: 677 794 20 4057 Fragments arranged by size: 4057 794 677 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5548 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5513 DraI TTT'AAA Cuts at: 2852 2852 Size: 5548 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4555 809 Size: 507 3239 1802 Fragments arranged by size: 3239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2017 2824 2995 Size: 54 1425 50 275 18 187 807 171 Cuts at: 2995 3884 5513 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 807 275 187 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2017 2824 2995 1537 Size: 275 205 807 171 4090 Fragments arranged by size: 4090 807 275 205 171 EarI CTCTTCn'nnn_ Cuts at: 1353 2738 3478 1353 Size: 1385 740 3423 Fragments arranged by size: 3423 1385 740 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2052 4135 4509 4655 387 Size: 1064 138 463 2083 374 146 1280 Fragments arranged by size: 2083 1280 1064 463 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2783 3611 4208 4995 5263 1239 Size: 393 1151 828 597 787 268 1524 Fragments arranged by size: 1524 1151 828 787 597 393 268 EcoRI G'AATT_C Cuts at: 2857 3235 2857 Size: 378 5170 EcoRV GAT'ATC Cuts at: 2107 2251 2107 Size: 144 5404 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2566 2860 2566 Size: 294 5254 FspI TGC'GCA Cuts at: 4054 4054 Size: 5548 HaeII r_GCGC'y Cuts at: 1678 2428 2788 4695 1678 Size: 750 360 1907 2531 Fragments arranged by size: 2531 1907 750 360 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2644 2676 2731 2763 4189 Size: 32 135 32 1072 32 55 32 1426 Cuts at: 4189 4221 4263 4295 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1426 1072 135 55 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2892 3138 669 Size: 988 281 954 246 3079 Fragments arranged by size: 3079 988 954 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5548 HpaI GTT'AAC Cuts at: 3138 3138 Size: 5548 KasI G'GCGC_C Cuts at: 2784 2784 Size: 5548 KpnI G_GTAC'C Cuts at: 2873 2873 Size: 5548 MfeI C'AATT_G Cuts at: 3147 3147 Size: 5548 MluI A'CGCG_T Cuts at: 5424 5424 Size: 5548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2496 4662 4846 1305 Size: 1191 2166 184 2007 Fragments arranged by size: 2166 2007 1191 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3726 Fragments arranged by size: 3726 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3506 3865 4024 518 Size: 648 2340 359 159 2042 Fragments arranged by size: 2340 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2016 2491 2964 3641 4789 5034 1606 Size: 410 475 473 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 475 473 410 245 NaeI GCC'GGC Cuts at: 2021 2021 Size: 5548 NarI GG'CG_CC Cuts at: 2785 2785 Size: 5548 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4057 Fragments arranged by size: 4057 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5548 NgoMIV G'CCGG_C Cuts at: 2019 2019 Size: 5548 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5548 NotI GC'GGCC_GC Cuts at: 2995 2995 Size: 5548 NspI r_CATG'y Cuts at: 1132 1229 2906 4451 1132 Size: 97 1677 1545 2229 Fragments arranged by size: 2229 1677 1545 97 PasI CC'CwG_GG Cuts at: 1348 2679 1348 Size: 1331 4217 PciI A'CATG_T Cuts at: 4447 4447 Size: 5548 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5548 PmeI GTTT'AAAC Cuts at: 2852 2852 Size: 5548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2675 2707 3617 3649 5130 5162 2675 Size: 32 910 32 1481 32 3061 Fragments arranged by size: 3061 1481 910 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5548 PsiI TTA'TAA Cuts at: 3118 3118 Size: 5548 PstI C_TGCA'G Cuts at: 830 2490 2900 830 Size: 1660 410 3478 Fragments arranged by size: 3478 1660 410 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5548 PvuII CAG'CTG Cuts at: 2491 2491 Size: 5548 SacII CC_GC'GG Cuts at: 2017 2017 Size: 5548 SalI G'TCGA_C Cuts at: 1936 2890 1936 Size: 954 4594 SbfI CC_TGCA'GG Cuts at: 2900 2900 Size: 5548 ScaI AGT'ACT Cuts at: 3796 5508 3796 Size: 1712 3836 SfcI C'TryA_G Cuts at: 826 1045 2486 2896 4031 4712 4903 826 Size: 219 1441 410 1135 681 191 1471 Fragments arranged by size: 1471 1441 1135 681 410 219 191 SfoI GGC'GCC Cuts at: 2786 2786 Size: 5548 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5548 SmaI CCC'GGG Cuts at: 1872 2875 1872 Size: 1003 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3652 4553 4815 820 Size: 197 926 1709 901 262 1553 Fragments arranged by size: 1709 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3339 493 Size: 2846 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5548 SphI G_CATG'C Cuts at: 1132 2906 1132 Size: 1774 3774 SspI AAT'ATT Cuts at: 5 52 3472 5 Size: 47 3420 2081 Fragments arranged by size: 3420 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5548 StyI C'CwwG_G Cuts at: 513 1065 2004 2987 513 Size: 552 939 983 3074 Fragments arranged by size: 3074 983 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2227 2298 2746 2828 3575 3592 3745 Size: 1255 647 71 448 82 747 17 153 Cuts at: 3745 3930 4375 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 747 647 448 445 185 153 82 71 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2430 2817 3794 Size: 274 80 33 51 1443 453 387 977 Cuts at: 3794 5506 96 Size: 1712 138 Fragments arranged by size: 1712 1443 977 453 387 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5508 5540 5508 Size: 32 5516 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5548 XbaI T'CTAG_A Cuts at: 2884 2884 Size: 5548 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5548 XmaI C'CCGG_G Cuts at: 1870 2873 1870 Size: 1003 4545 XmnI GAAnn'nnTTC Cuts at: 3677 3677 Size: 5548 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3356 276 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI