(Circular) (Six-base) MAPSORT of: pFN21AE0597.seq Check: 78 from: 1 to: 9154 pFN21AE0597 9154 bp With 182 enzymes: SgfI * August 20, 2012 11:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3181 3181 Size: 9154 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4229 6964 278 Size: 53 83 186 936 2693 2735 2468 Fragments arranged by size: 2735 2693 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6497 1937 Size: 4560 4594 Acc65I G'GTAC_C Cuts at: 5076 6475 5076 Size: 1399 7755 AclI AA'CG_TT Cuts at: 1563 7281 7654 1563 Size: 5718 373 3063 Fragments arranged by size: 5718 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3111 3308 3642 3822 4530 4638 5288 5763 Size: 1479 197 334 180 708 108 650 475 Cuts at: 5763 5984 6067 6079 6411 7217 8601 8869 1632 Size: 221 83 12 332 806 1384 268 1917 Fragments arranged by size: 1917 1479 1384 806 708 650 475 334 332 268 221 197 180 108 83 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8957 AflIII A'CryG_T Cuts at: 1203 8053 9030 1203 Size: 6850 977 1327 Fragments arranged by size: 6850 1327 977 AgeI A'CCGG_T Cuts at: 7985 7985 Size: 9154 AhdI GACnn_n'nnGTC Cuts at: 7883 7883 Size: 9154 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9154 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4338 4559 6293 1184 Size: 3154 221 1734 4045 Fragments arranged by size: 4045 3154 1734 221 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4560 4592 6167 6199 4560 Size: 32 1575 32 7515 Fragments arranged by size: 7515 1575 32 32 AlwNI CAG_nnn'CTG Cuts at: 4508 4652 4844 6897 8469 4508 Size: 144 192 2053 1572 5193 Fragments arranged by size: 5193 2053 1572 192 144 ApaLI G'TGCA_C Cuts at: 7211 8367 7211 Size: 1156 7998 ApoI r'AATT_y Cuts at: 1493 2651 3076 3262 3370 3848 4211 5119 5240 Size: 1158 425 186 108 478 363 908 121 Cuts at: 5240 5324 6463 6689 6841 8950 1493 Size: 84 1139 226 152 2109 1697 Fragments arranged by size: 2109 1697 1158 1139 908 478 425 363 226 186 152 121 108 84 AseI AT'TA_AT Cuts at: 160 1032 2574 7708 160 Size: 872 1542 5134 1606 Fragments arranged by size: 5134 1606 1542 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9154 AvaI C'yCGr_G Cuts at: 1870 1943 2106 5250 5917 6479 1870 Size: 73 163 3144 667 562 4545 Fragments arranged by size: 4545 3144 667 562 163 73 AvrII C'CTAG_G Cuts at: 3386 3386 Size: 9154 BamHI G'GATC_C Cuts at: 2936 4269 6484 6851 2936 Size: 1333 2215 367 5239 Fragments arranged by size: 5239 2215 1333 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2465 5076 5913 5959 Size: 325 217 627 221 457 2611 837 46 Cuts at: 5959 6475 7930 618 Size: 516 1455 1842 Fragments arranged by size: 2611 1842 1455 837 627 516 457 325 221 217 46 BanII G_rGCy'C Cuts at: 1391 2644 3364 3541 4127 4491 6017 6473 1391 Size: 1253 720 177 586 364 1526 456 4072 Fragments arranged by size: 4072 1526 1253 720 586 456 364 177 BbeI G_GCGC'C Cuts at: 2012 5917 2012 Size: 3905 5249 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3296 3509 4017 4518 5015 5468 6302 Size: 971 1397 213 508 501 497 453 834 Cuts at: 6302 6448 928 Size: 146 3634 Fragments arranged by size: 3634 1397 971 834 508 501 497 453 213 146 BbvCI CC'TCA_GC Cuts at: 5979 6249 5979 Size: 270 8884 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2574 2608 7345 7379 1903 Size: 34 637 34 4737 34 3678 Fragments arranged by size: 4737 3678 637 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3787 3903 7046 8256 3787 Size: 116 3143 1210 4685 Fragments arranged by size: 4685 3143 1210 116 BclI T'GATC_A Cuts at: 1548 6141 1548 Size: 4593 4561 BfrBI ATG'CAT Cuts at: 2786 2786 Size: 9154 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5306 7765 136 Size: 107 122 71 631 605 3634 2459 1525 Fragments arranged by size: 3634 2459 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4097 4245 9149 4097 Size: 148 4904 4102 Fragments arranged by size: 4904 4102 148 BlpI GC'TnA_GC Cuts at: 2223 6281 6571 2223 Size: 4058 290 4806 Fragments arranged by size: 4806 4058 290 Bme1580I G_kGCm'C Cuts at: 1790 5690 7215 8371 1790 Size: 3900 1525 1156 2573 Fragments arranged by size: 3900 2573 1525 1156 BmgBI CAC'GTC Cuts at: 1318 2157 1318 Size: 839 8315 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6885 7843 442 Size: 416 53 83 399 24 5468 958 1753 Fragments arranged by size: 5468 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9154 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3560 3560 Size: 9154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2485 4220 5471 5476 6053 7814 1239 Size: 1246 1735 1251 5 577 1761 2579 Fragments arranged by size: 2579 1761 1735 1251 1246 577 5 Bpu10I CC'TnA_GC Cuts at: 2514 5979 6249 2514 Size: 3465 270 5419 Fragments arranged by size: 5419 3465 270 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2378 3737 3874 3946 7279 8144 8442 2378 Size: 1359 137 72 3333 865 298 3090 Fragments arranged by size: 3333 3090 1359 865 298 137 72 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4196 4298 5845 5952 7817 882 Size: 617 2697 102 1547 107 1865 2219 Fragments arranged by size: 2697 2219 1865 1547 617 107 102 BsaAI yAC'GTr Cuts at: 493 1204 2588 3022 5503 6945 493 Size: 711 1384 434 2481 1442 2702 Fragments arranged by size: 2702 2481 1442 1384 711 434 BsaBI GATnn'nnATC Cuts at: 9148 9148 Size: 9154 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 4226 5914 6961 Size: 53 83 186 936 476 2217 1688 1047 Cuts at: 6961 7343 7978 275 Size: 382 635 1451 Fragments arranged by size: 2217 1688 1451 1047 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4266 7587 7985 8259 8406 8817 4266 Size: 3321 398 274 147 411 4603 Fragments arranged by size: 4603 3321 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2279 2309 2554 2584 3972 Size: 30 165 30 876 30 245 30 1388 Cuts at: 3972 4002 1178 Size: 30 6330 Fragments arranged by size: 6330 1388 876 245 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2252 2634 3493 3674 4247 5804 5807 Size: 889 177 382 859 181 573 1557 3 Cuts at: 5807 5810 5822 5825 5828 1186 Size: 3 12 3 3 4512 Fragments arranged by size: 4512 1557 889 859 573 382 181 177 12 3 3 3 3 BseYI C'CCAG_C Cuts at: 2858 4785 5689 8357 8853 2858 Size: 1927 904 2668 496 3159 Fragments arranged by size: 3159 2668 1927 904 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3122 3951 5430 6191 6227 1163 Size: 573 1386 829 1479 761 36 4090 Fragments arranged by size: 4090 1479 1386 829 761 573 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6604 7365 8393 1540 Size: 275 186 4603 761 1028 2301 Fragments arranged by size: 4603 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2587 3541 3597 6017 7215 7300 8371 2587 Size: 954 56 2420 1198 85 1071 3370 Fragments arranged by size: 3370 2420 1198 1071 954 85 56 BsmI GAATG_Cn' Cuts at: 3779 4060 6665 6758 3779 Size: 281 2605 93 6175 Fragments arranged by size: 6175 2605 281 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3548 3548 Size: 9154 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2587 2644 3364 3541 3597 4127 4491 Size: 399 797 57 720 177 56 530 364 Cuts at: 4491 5690 6017 6473 7215 7300 8371 1391 Size: 1199 327 456 742 85 1071 2174 Fragments arranged by size: 2174 1199 1071 797 742 720 530 456 399 364 327 177 85 57 56 BspEI T'CCGG_A Cuts at: 8817 8817 Size: 9154 BspHI T'CATG_A Cuts at: 2334 4275 7043 8773 2334 Size: 1941 2768 1730 2715 Fragments arranged by size: 2768 2715 1941 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3181 6509 844 Size: 2337 3328 3489 Fragments arranged by size: 3489 3328 2337 BsrBI CCG'CTC Cuts at: 1394 1823 2014 2068 6201 7041 1394 Size: 429 191 54 4133 840 3507 Fragments arranged by size: 4133 3507 840 429 191 54 BsrDI GCAATG_nn' Cuts at: 65 1248 3438 7649 7823 65 Size: 1183 2190 4211 174 1396 Fragments arranged by size: 4211 2190 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5650 5910 7798 7985 1700 Size: 110 3840 260 1888 187 2869 Fragments arranged by size: 3840 2869 1888 260 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9154 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9154 BssSI C'ACGA_G Cuts at: 3357 7214 8226 3357 Size: 3857 1012 4285 Fragments arranged by size: 4285 3857 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5438 5506 5555 794 Size: 4644 68 49 4393 Fragments arranged by size: 4644 4393 68 49 BstBI TT'CG_AA Cuts at: 7958 7958 Size: 9154 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9154 BstXI CCAn_nnnn'nTGG Cuts at: 3844 3844 Size: 9154 BstYI r'GATC_y Cuts at: 1972 2936 4097 4245 4269 6054 6484 6851 7236 Size: 964 1161 148 24 1785 430 367 385 Cuts at: 7236 7253 8705 8791 8803 9149 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1785 1452 1161 964 430 385 367 346 148 86 24 17 12 Bsu36I CC'TnA_GG Cuts at: 5031 5031 Size: 9154 BtgI C'CryG_G Cuts at: 513 1065 2004 3365 3837 5725 6153 6265 513 Size: 552 939 1361 472 1888 428 112 3402 Fragments arranged by size: 3402 1888 1361 939 552 472 428 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8415 Fragments arranged by size: 8415 683 56 BtsI GCAGTG_nn' Cuts at: 4822 5690 6670 7464 7484 4822 Size: 868 980 794 20 6492 Fragments arranged by size: 6492 980 868 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9154 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5621 5656 5707 5742 6247 6282 529 Size: 35 5057 35 51 35 505 35 3401 Fragments arranged by size: 5057 3401 505 51 35 35 35 35 DraI TTT'AAA Cuts at: 5160 6458 5160 Size: 1298 7856 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4838 8161 809 Size: 507 3522 3323 1802 Fragments arranged by size: 3522 3323 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3333 3834 6340 Size: 54 1425 50 275 18 1503 501 2506 Cuts at: 6340 6601 7490 9119 8 Size: 261 889 1629 43 Fragments arranged by size: 2506 1629 1503 1425 889 501 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6601 1537 Size: 275 4789 4090 Fragments arranged by size: 4789 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2075 2106 2352 2433 2986 3694 4370 4526 Size: 722 31 246 81 553 708 676 156 Cuts at: 4526 4751 5066 5403 5739 5781 5799 6045 7084 Size: 225 315 337 336 42 18 246 1039 Cuts at: 7084 1353 Size: 3423 Fragments arranged by size: 3423 1039 722 708 676 553 337 336 315 246 246 225 156 81 42 31 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7741 8115 8261 387 Size: 1064 138 6152 374 146 1280 Fragments arranged by size: 6152 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3539 6015 3539 Size: 2476 6678 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2485 3111 3308 3642 3822 4220 4530 Size: 393 853 626 197 334 180 398 310 Cuts at: 4530 4638 5288 5471 5476 5763 5984 6053 6067 Size: 108 650 183 5 287 221 69 14 Cuts at: 6067 6079 6411 7217 7814 8601 8869 1239 Size: 12 332 806 597 787 268 1524 Fragments arranged by size: 1524 853 806 787 650 626 597 398 393 334 332 310 287 268 221 197 183 180 108 69 14 12 5 EcoNI CCTnn'n_nnAGG Cuts at: 3162 3162 Size: 9154 EcoO109I rG'GnC_Cy Cuts at: 3390 3390 Size: 9154 EcoRI G'AATT_C Cuts at: 3076 3262 3370 3848 6463 6841 3076 Size: 186 108 478 2615 378 5389 Fragments arranged by size: 5389 2615 478 378 186 108 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3731 3961 5231 6097 3731 Size: 230 1270 866 6788 Fragments arranged by size: 6788 1270 866 230 FseI GG_CCGG'CC Cuts at: 5654 5654 Size: 9154 FspI TGC'GCA Cuts at: 4094 4700 7660 4094 Size: 606 2960 5588 Fragments arranged by size: 5588 2960 606 HaeII r_GCGC'y Cuts at: 1678 2012 2743 3486 5917 8301 1678 Size: 334 731 743 2431 2384 2531 Fragments arranged by size: 2531 2431 2384 743 731 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2441 2465 2473 2497 4211 Size: 32 135 32 869 24 8 24 1714 Cuts at: 4211 4243 4728 4760 7795 7827 7869 7901 1373 Size: 32 485 32 3035 32 42 32 2626 Fragments arranged by size: 3035 2626 1714 869 485 135 42 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 3759 5051 6498 6744 669 Size: 988 281 1821 1292 1447 246 3079 Fragments arranged by size: 3079 1821 1447 1292 988 281 246 HindIII A'AGCT_T Cuts at: 748 2261 4659 748 Size: 1513 2398 5243 Fragments arranged by size: 5243 2398 1513 HpaI GTT'AAC Cuts at: 3759 6744 3759 Size: 2985 6169 KasI G'GCGC_C Cuts at: 2008 5913 2008 Size: 3905 5249 KpnI G_GTAC'C Cuts at: 5080 6479 5080 Size: 1399 7755 MfeI C'AATT_G Cuts at: 6753 6753 Size: 9154 MluI A'CGCG_T Cuts at: 9030 9030 Size: 9154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2307 3306 6403 8268 8452 1305 Size: 1002 999 3097 1865 184 2007 Fragments arranged by size: 3097 2007 1865 1002 999 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3335 3836 6342 10 Size: 54 1425 343 1503 501 2506 2822 Fragments arranged by size: 2822 2506 1503 1425 501 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2667 3842 6348 7112 7471 7630 518 Size: 648 1501 1175 2506 764 359 159 2042 Fragments arranged by size: 2506 2042 1501 1175 764 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 4154 4649 6087 6570 7247 8395 8640 Size: 432 2116 495 1438 483 677 1148 245 Cuts at: 8640 1606 Size: 2120 Fragments arranged by size: 2120 2116 1438 1148 677 495 483 432 245 NaeI GCC'GGC Cuts at: 5652 5912 5652 Size: 260 8894 NarI GG'CG_CC Cuts at: 2009 5914 2009 Size: 3905 5249 NcoI C'CATG_G Cuts at: 513 1065 2004 3837 6153 513 Size: 552 939 1833 2316 3514 Fragments arranged by size: 3514 2316 1833 939 552 NdeI CA'TA_TG Cuts at: 387 6349 387 Size: 5962 3192 NgoMIV G'CCGG_C Cuts at: 5650 5910 5650 Size: 260 8894 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9154 NotI GC'GGCC_GC Cuts at: 6601 6601 Size: 9154 NsiI A_TGCA'T Cuts at: 2788 2788 Size: 9154 NspI r_CATG'y Cuts at: 1132 1229 6512 8057 1132 Size: 97 5283 1545 2229 Fragments arranged by size: 5283 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9154 PciI A'CATG_T Cuts at: 8053 8053 Size: 9154 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9154 PfoI T'CCnGG_A Cuts at: 4578 4665 4762 5472 5492 6017 4578 Size: 87 97 710 20 525 7715 Fragments arranged by size: 7715 710 525 97 87 20 PmeI GTTT'AAAC Cuts at: 6458 6458 Size: 9154 PmlI CAC'GTG Cuts at: 5503 5503 Size: 9154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7223 7255 8736 8768 7223 Size: 32 1481 32 7609 Fragments arranged by size: 7609 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3390 3390 Size: 9154 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9154 PsiI TTA'TAA Cuts at: 6724 6724 Size: 9154 PstI C_TGCA'G Cuts at: 830 3427 4327 5899 6042 6506 830 Size: 2597 900 1572 143 464 3478 Fragments arranged by size: 3478 2597 1572 900 464 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9154 PvuII CAG'CTG Cuts at: 4154 4649 6087 4154 Size: 495 1438 7221 Fragments arranged by size: 7221 1438 495 SacI G_AGCT'C Cuts at: 3541 6017 3541 Size: 2476 6678 SalI G'TCGA_C Cuts at: 1936 6496 1936 Size: 4560 4594 SapI GCTCTTCn'nnn_ Cuts at: 2075 4526 4751 2075 Size: 2451 225 6478 Fragments arranged by size: 6478 2451 225 SbfI CC_TGCA'GG Cuts at: 6506 6506 Size: 9154 ScaI AGT'ACT Cuts at: 2167 7402 9114 2167 Size: 5235 1712 2207 Fragments arranged by size: 5235 2207 1712 SfcI C'TryA_G Cuts at: 826 1045 3423 4241 4323 4938 5895 6038 6502 Size: 219 2378 818 82 615 957 143 464 Cuts at: 6502 7637 8318 8509 826 Size: 1135 681 191 1471 Fragments arranged by size: 2378 1471 1135 957 818 681 615 464 219 191 143 82 SfoI GGC'GCC Cuts at: 2010 5915 2010 Size: 3905 5249 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9154 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9154 SmaI CCC'GGG Cuts at: 1872 2108 5252 5919 6481 1872 Size: 236 3144 667 562 4545 Fragments arranged by size: 4545 3144 667 562 236 SmlI C'TyrA_G Cuts at: 820 1017 1943 2393 3716 3853 3925 7258 8159 Size: 197 926 450 1323 137 72 3333 901 Cuts at: 8159 8421 820 Size: 262 1553 Fragments arranged by size: 3333 1553 1323 926 901 450 262 197 137 72 SnaBI TAC'GTA Cuts at: 493 6945 493 Size: 6452 2702 SpeI A'CTAG_T Cuts at: 152 4690 152 Size: 4538 4616 SphI G_CATG'C Cuts at: 1132 6512 1132 Size: 5380 3774 SspI AAT'ATT Cuts at: 5 52 3641 7078 5 Size: 47 3589 3437 2081 Fragments arranged by size: 3589 3437 2081 47 StuI AGG'CCT Cuts at: 1517 2096 1517 Size: 579 8575 StyI C'CwwG_G Cuts at: 513 1065 2004 2816 3386 3837 4680 5332 5955 Size: 552 939 812 570 451 843 652 623 Cuts at: 5955 6153 6593 513 Size: 198 440 3074 Fragments arranged by size: 3074 939 843 812 652 623 570 552 451 440 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4969 6360 7181 7198 7351 7536 7981 Size: 1255 3389 1391 821 17 153 185 445 Cuts at: 7981 325 Size: 1498 Fragments arranged by size: 3389 1498 1391 1255 821 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2165 7400 9112 Size: 274 80 33 51 1443 188 5235 1712 Cuts at: 9112 96 Size: 138 Fragments arranged by size: 5235 1712 1443 274 188 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3181 3213 9114 9146 3181 Size: 32 5901 32 3189 Fragments arranged by size: 5901 3189 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9154 XbaI T'CTAG_A Cuts at: 3100 3589 6490 3100 Size: 489 2901 5764 Fragments arranged by size: 5764 2901 489 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2347 3758 4841 5676 2347 Size: 1411 1083 835 5825 Fragments arranged by size: 5825 1411 1083 835 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9154 XmaI C'CCGG_G Cuts at: 1870 2106 5250 5917 6479 1870 Size: 236 3144 667 562 4545 Fragments arranged by size: 4545 3144 667 562 236 XmnI GAAnn'nnTTC Cuts at: 2277 4661 7283 2277 Size: 2384 2622 4148 Fragments arranged by size: 4148 2622 2384 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4227 6962 276 Size: 53 83 186 936 2693 2735 2468 Fragments arranged by size: 2735 2693 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BsiWI BstZ17I DraIII EcoRV FspAI NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI