(Circular) (Six-base) MAPSORT of: pFN21AE5241.seq Check: 1634 from: 1 to: 5455 pFN21AE5241 5455 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3265 278 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2798 1937 Size: 861 4594 Acc65I G'GTAC_C Cuts at: 2776 2776 Size: 5455 AclI AA'CG_TT Cuts at: 1563 3582 3955 1563 Size: 2019 373 3063 Fragments arranged by size: 3063 2019 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3518 4902 5170 1632 Size: 1886 1384 268 1917 Fragments arranged by size: 1917 1886 1384 268 AfeI AGC'GCT Cuts at: 2081 2081 Size: 5455 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5258 AflIII A'CryG_T Cuts at: 1203 4354 5331 1203 Size: 3151 977 1327 Fragments arranged by size: 3151 1327 977 AgeI A'CCGG_T Cuts at: 4286 4286 Size: 5455 AhdI GACnn_n'nnGTC Cuts at: 4184 4184 Size: 5455 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5455 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5455 AlwNI CAG_nnn'CTG Cuts at: 3198 4770 3198 Size: 1572 3883 ApaLI G'TGCA_C Cuts at: 3512 4668 3512 Size: 1156 4299 ApoI r'AATT_y Cuts at: 1493 2747 2764 2990 3142 5251 1493 Size: 1254 17 226 152 2109 1697 Fragments arranged by size: 2109 1697 1254 226 152 17 AseI AT'TA_AT Cuts at: 160 1032 4009 160 Size: 872 2977 1606 Fragments arranged by size: 2977 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 AvaI C'yCGr_G Cuts at: 1870 1943 2644 2780 1870 Size: 73 701 136 4545 Fragments arranged by size: 4545 701 136 73 BamHI G'GATC_C Cuts at: 2785 3152 2785 Size: 367 5088 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2776 4231 618 Size: 325 217 627 989 1455 1842 Fragments arranged by size: 1842 1455 989 627 325 217 BanII G_rGCy'C Cuts at: 1391 2774 1391 Size: 1383 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4484 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3646 3680 1903 Size: 34 1709 34 3678 Fragments arranged by size: 3678 1709 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3347 4557 3347 Size: 1210 4245 BclI T'GATC_A Cuts at: 1548 2583 1548 Size: 1035 4420 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4066 136 Size: 107 122 71 631 605 2394 1525 Fragments arranged by size: 2394 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2212 5450 2212 Size: 3238 2217 BlpI GC'TnA_GC Cuts at: 2872 2872 Size: 5455 Bme1580I G_kGCm'C Cuts at: 1790 3516 4672 1790 Size: 1726 1156 2573 Fragments arranged by size: 2573 1726 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5455 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3186 4144 442 Size: 416 53 83 399 24 1769 958 1753 Fragments arranged by size: 1769 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5455 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 4115 1239 Size: 1097 1779 2579 Fragments arranged by size: 2579 1779 1097 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2558 3580 4445 4743 2558 Size: 1022 865 298 3270 Fragments arranged by size: 3270 1022 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2374 4118 882 Size: 617 875 1744 2219 Fragments arranged by size: 2219 1744 875 617 BsaAI yAC'GTr Cuts at: 493 1204 3246 493 Size: 711 2042 2702 Fragments arranged by size: 2702 2042 711 BsaBI GATnn'nnATC Cuts at: 5449 5449 Size: 5455 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3262 3644 4279 275 Size: 53 83 186 936 1729 382 635 1451 Fragments arranged by size: 1729 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3888 4286 4560 4707 5118 3888 Size: 398 274 147 411 4225 Fragments arranged by size: 4225 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2309 2339 1178 Size: 30 165 30 906 30 4294 Fragments arranged by size: 4294 906 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5455 BseYI C'CCAG_C Cuts at: 4658 5154 4658 Size: 496 4959 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4882 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2905 3666 4694 1540 Size: 275 186 904 761 1028 2301 Fragments arranged by size: 2301 1028 904 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2515 3516 3601 4672 2515 Size: 1001 85 1071 3298 Fragments arranged by size: 3298 1071 1001 85 BsmI GAATG_Cn' Cuts at: 2966 3059 2966 Size: 93 5362 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2515 2774 3516 3601 4672 1391 Size: 399 725 259 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 725 399 259 85 BspEI T'CCGG_A Cuts at: 5118 5118 Size: 5455 BspHI T'CATG_A Cuts at: 3344 5074 3344 Size: 1730 3725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2810 844 Size: 1966 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2127 3342 1394 Size: 429 304 1215 3507 Fragments arranged by size: 3507 1215 429 304 BsrDI GCAATG_nn' Cuts at: 65 1248 2334 3950 4124 65 Size: 1183 1086 1616 174 1396 Fragments arranged by size: 1616 1396 1183 1086 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4099 4286 1700 Size: 110 2289 187 2869 Fragments arranged by size: 2869 2289 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5455 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5455 BssSI C'ACGA_G Cuts at: 3515 4527 3515 Size: 1012 4443 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5455 BstBI TT'CG_AA Cuts at: 2085 4259 2085 Size: 2174 3281 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5455 BstYI r'GATC_y Cuts at: 1972 2031 2212 2785 3152 3537 3554 5006 5092 Size: 59 181 573 367 385 17 1452 86 Cuts at: 5092 5104 5450 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 573 385 367 346 181 86 59 17 12 BtgI C'CryG_G Cuts at: 513 1065 2052 513 Size: 552 987 3916 Fragments arranged by size: 3916 987 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4716 Fragments arranged by size: 4716 683 56 BtsI GCAGTG_nn' Cuts at: 2971 3765 3785 2971 Size: 794 20 4641 Fragments arranged by size: 4641 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5455 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2328 2363 529 Size: 35 1764 35 3621 Fragments arranged by size: 3621 1764 35 35 DraI TTT'AAA Cuts at: 2759 2759 Size: 5455 DraIII CAC_nnn'GTG Cuts at: 2170 2170 Size: 5455 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4462 809 Size: 507 3146 1802 Fragments arranged by size: 3146 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2611 2902 Size: 54 1425 50 275 18 219 562 291 Cuts at: 2902 3791 5420 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 562 291 275 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2902 1537 Size: 275 1090 4090 Fragments arranged by size: 4090 1090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3385 1353 Size: 2032 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2137 4042 4416 4562 387 Size: 1064 138 435 113 1905 374 146 1280 Fragments arranged by size: 1905 1280 1064 435 374 146 138 113 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 3518 4115 4902 5170 1239 Size: 393 704 1182 597 787 268 1524 Fragments arranged by size: 1524 1182 787 704 597 393 268 EcoRI G'AATT_C Cuts at: 2764 3142 2764 Size: 378 5077 FspI TGC'GCA Cuts at: 3961 3961 Size: 5455 HaeII r_GCGC'y Cuts at: 1678 2083 4602 1678 Size: 405 2519 2531 Fragments arranged by size: 2531 2519 405 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4096 4128 4170 4202 1373 Size: 32 135 32 2524 32 42 32 2626 Fragments arranged by size: 2626 2524 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2799 3045 669 Size: 988 281 861 246 3079 Fragments arranged by size: 3079 988 861 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5455 HpaI GTT'AAC Cuts at: 3045 3045 Size: 5455 KpnI G_GTAC'C Cuts at: 2780 2780 Size: 5455 MfeI C'AATT_G Cuts at: 3054 3054 Size: 5455 MluI A'CGCG_T Cuts at: 5331 5331 Size: 5455 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4569 4753 1305 Size: 3264 184 2007 Fragments arranged by size: 3264 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2613 10 Size: 54 1425 343 781 2852 Fragments arranged by size: 2852 1425 781 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3413 3772 3931 518 Size: 648 2247 359 159 2042 Fragments arranged by size: 2247 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2871 3548 4696 4941 1606 Size: 1265 677 1148 245 2120 Fragments arranged by size: 2120 1265 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4903 NdeI CA'TA_TG Cuts at: 387 387 Size: 5455 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5455 NotI GC'GGCC_GC Cuts at: 2902 2902 Size: 5455 NspI r_CATG'y Cuts at: 1132 1229 2813 4358 1132 Size: 97 1584 1545 2229 Fragments arranged by size: 2229 1584 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5455 PciI A'CATG_T Cuts at: 4354 4354 Size: 5455 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5455 PmeI GTTT'AAAC Cuts at: 2759 2759 Size: 5455 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3524 3556 5037 5069 3524 Size: 32 1481 32 3910 Fragments arranged by size: 3910 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5455 PsiI TTA'TAA Cuts at: 3025 3025 Size: 5455 PstI C_TGCA'G Cuts at: 830 2160 2807 830 Size: 1330 647 3478 Fragments arranged by size: 3478 1330 647 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5455 SalI G'TCGA_C Cuts at: 1936 2797 1936 Size: 861 4594 SbfI CC_TGCA'GG Cuts at: 2807 2807 Size: 5455 ScaI AGT'ACT Cuts at: 2712 3703 5415 2712 Size: 991 1712 2752 Fragments arranged by size: 2752 1712 991 SfcI C'TryA_G Cuts at: 826 1045 2156 2803 3938 4619 4810 826 Size: 219 1111 647 1135 681 191 1471 Fragments arranged by size: 1471 1135 1111 681 647 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5455 SmaI CCC'GGG Cuts at: 1872 2646 2782 1872 Size: 774 136 4545 Fragments arranged by size: 4545 774 136 SmlI C'TyrA_G Cuts at: 820 1017 1943 2573 3559 4460 4722 820 Size: 197 926 630 986 901 262 1553 Fragments arranged by size: 1553 986 926 901 630 262 197 SnaBI TAC'GTA Cuts at: 493 3246 493 Size: 2753 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5455 SphI G_CATG'C Cuts at: 1132 2813 1132 Size: 1681 3774 SspI AAT'ATT Cuts at: 5 52 3379 5 Size: 47 3327 2081 Fragments arranged by size: 3327 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5455 StyI C'CwwG_G Cuts at: 513 1065 2894 513 Size: 552 1829 3074 Fragments arranged by size: 3074 1829 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3482 3499 3652 3837 4282 325 Size: 1255 1902 17 153 185 445 1498 Fragments arranged by size: 1902 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2450 2710 3701 Size: 274 80 33 51 1443 473 260 991 Cuts at: 3701 5413 96 Size: 1712 138 Fragments arranged by size: 1712 1443 991 473 274 260 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2053 2085 5415 5447 2053 Size: 32 3330 32 2061 Fragments arranged by size: 3330 2061 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5455 XbaI T'CTAG_A Cuts at: 2791 2791 Size: 5455 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2050 2050 Size: 5455 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5455 XmaI C'CCGG_G Cuts at: 1870 2644 2780 1870 Size: 774 136 4545 Fragments arranged by size: 4545 774 136 XmnI GAAnn'nnTTC Cuts at: 2081 3584 2081 Size: 1503 3952 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3263 276 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI