Restriction map for
pFN21AE6624
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
Alw44I AlwI AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BcgI BciVI BfrI BglI
BglII BlnI BmyI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I Cfr10I
Cfr13I CpoI DraII DraIII EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeIII Hin1I
HpaI KasI KpnI Ksp632I KspI
MaeII MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AccII 126 CGCG CG^CG
AhdI 124 GACGCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 41 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 191 AGCGCT AGC^GCT
AspEI 124 GACGCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 538 GGTGCC G^G[CT][GA]CC
BbvCI 18 GCTGAGG CCTCAGC(-5/-2)
BclI 599 TGATCA T^GATCA
BcnI 265 CCCGG CC^[GC]GG
BfaI 100 CTAG C^TAG
BlpI 194 GCTTAGC GC^T[ACGT]AGC
BpmI 213 CTCCAG CTGGAG(16/14)
Bpu1102I 194 GCTTAGC GC^T[ACGT]AGC
BsaI 3 GGTCTC GGTCTC(1/5)
BsgI 226 GTGCAG GTGCAG(16/14)
BsmBI 442 GAGACG CGTCTC(1/5)
BspDI 91 ATCGAT AT^CGAT
BspHI 524 TCATGA T^CATGA
BsrI 666 ACTGG ACTGG(1/-1)
BstF5I 204 CATCC GGATG(2/0)
BstNI 215 CCAGG CC^[AT]GG
BstUI 126 CGCG CG^CG
BstXI 542 CCACAGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 194 GCTTAGC GC^T[ACGT]AGC
CfoI 192 GCGC GCG^C
ClaI 91 ATCGAT AT^CGAT
DpnII 600 GATC ^GATC
DraI 492 TTTAAA TTT^AAA
DrdI 64 GACAGCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 152 CCATGG C^C[GA][CT]GG
Eam1105I 124 GACGCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 191 AGCGCT AGC^GCT
EcoRII 215 CCAGG ^CC[AT]GG
EcoT14I 152 CCATGG C^C[AT][AT]GG
FauI 158 GCGGG CCCGC(4/6)
FbaI 599 TGATCA T^GATCA
FokI 204 CATCC GGATG(9/13)
HaeII 191 AGCGCT [GA]GCGC^[CT]
HapII 266 CCGG C^CGG
HhaI 192 GCGC GCG^C
HinP1I 192 GCGC G^CGC
HincII 61 GTTGAC GT[CT]^[GA]AC
HindII 61 GTTGAC GT[CT]^[GA]AC
HindIII 25 AAGCTT A^AGCTT
HpaII 266 CCGG C^CGG
HphI 421 GGTGA GGTGA(8/7)
MaeI 100 CTAG C^TAG
MboI 600 GATC ^GATC
MslI 543 CACAGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 455 CAGCTG C[AC]G^C[GT]G
MspI 266 CCGG C^CGG
MvaI 215 CCAGG CC^[AT]GG
MvnI 126 CGCG CG^CG
MwoI 18 GCTGAGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 265 CCCGG CC^[GC]GG
NcoI 152 CCATGG C^CATGG
NdeII 600 GATC ^GATC
NheI 99 GCTAGC G^CTAGC
NlaIV 538 GGTGCC GG[ACGT]^[ACGT]CC
NspI 351 GCATGT [GA]CATG^[CT]
PleI 233 GACTC GAGTC(4/5)
PvuII 455 CAGCTG CAG^CTG
RcaI 524 TCATGA T^CATGA
Sau3AI 600 GATC ^GATC
SfcI 292 CTACAG C^T[GA][CT]AG
StyI 152 CCATGG C^C[AT][AT]GG
TaqI 92 TCGA T^CGA
TthHB8I 92 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 127 GCGG CCGC(-3/-1)
158 GCGG
AcsI 406 AAATTC [GA]^AATT[CT]
502 AAATTT
ApoI 406 AAATTC [GA]^AATT[CT]
502 AAATTT
Asp700I 24 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
499 GAAAAATTTC
BsiYI 152 CCATGGGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
266 CCGGCATAAGG
BslI 152 CCATGGGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
266 CCGGCATAAGG
BspMI 222 GCAGGT ACCTGC(4/8)
430 GCAGGT
BssKI 215 CCAGG ^CC[ACGT]GG
265 CCCGG
Cac8I 99 GCTAGC GC[ACGT]^[ACGT]GC
147 GCGAGC
HgaI 124 GACGC GACGC(5/10)
444 GACGC
MboII 82 GAAGA GAAGA(8/7)
670 GAAGA
ScrFI 215 CCAGG CC^[ACGT]GG
265 CCCGG
SfaNI 43 GATGC GCATC(5/9)
571 GATGC
TfiI 89 GAATC G^A[AT]TC
460 GAATC
Tsp509I 407 AATT ^AATT
503 AATT
TspRI 164 CAGTG CA[GC]TG[ACGT][ACGT]^
639 CACTG
XmnI 24 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
499 GAAAAATTTC
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
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AluI BbvI BsaJI BsmAI DdeI
Fnu4HI HinfI ItaI MnlI MseI
NlaIII Tru9I TseI