(Circular) (Six-base) MAPSORT of: pFN21AE6624.seq Check: 125 from: 1 to: 5380 pFN21AE6624 5380 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2218 2218 Size: 5380 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3190 278 Size: 53 83 186 936 1654 2468 Fragments arranged by size: 2468 1654 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2723 1937 Size: 786 4594 Acc65I G'GTAC_C Cuts at: 2701 2701 Size: 5380 AclI AA'CG_TT Cuts at: 1563 3507 3880 1563 Size: 1944 373 3063 Fragments arranged by size: 3063 1944 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3443 4827 5095 1632 Size: 1811 1384 268 1917 Fragments arranged by size: 1917 1811 1384 268 AfeI AGC'GCT Cuts at: 2198 2198 Size: 5380 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5183 AflIII A'CryG_T Cuts at: 1203 4279 5256 1203 Size: 3076 977 1327 Fragments arranged by size: 3076 1327 977 AgeI A'CCGG_T Cuts at: 4211 4211 Size: 5380 AhdI GACnn_n'nnGTC Cuts at: 2134 4109 2134 Size: 1975 3405 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5380 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5380 AlwNI CAG_nnn'CTG Cuts at: 2051 3123 4695 2051 Size: 1072 1572 2736 Fragments arranged by size: 2736 1572 1072 ApaLI G'TGCA_C Cuts at: 3437 4593 3437 Size: 1156 4224 ApoI r'AATT_y Cuts at: 1493 2411 2507 2689 2915 3067 5176 1493 Size: 918 96 182 226 152 2109 1697 Fragments arranged by size: 2109 1697 918 226 182 152 96 AseI AT'TA_AT Cuts at: 160 1032 3934 160 Size: 872 2902 1606 Fragments arranged by size: 2902 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5380 AvaI C'yCGr_G Cuts at: 1870 1943 2705 1870 Size: 73 762 4545 Fragments arranged by size: 4545 762 73 BamHI G'GATC_C Cuts at: 2710 3077 2710 Size: 367 5013 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2543 2701 4156 618 Size: 325 217 627 756 158 1455 1842 Fragments arranged by size: 1842 1455 756 627 325 217 158 BanII G_rGCy'C Cuts at: 1391 2699 1391 Size: 1308 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4409 BbvCI CC'TCA_GC Cuts at: 2024 2024 Size: 5380 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3571 3605 1903 Size: 34 1634 34 3678 Fragments arranged by size: 3678 1634 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3272 4482 3272 Size: 1210 4170 BclI T'GATC_A Cuts at: 1548 2604 1548 Size: 1056 4324 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3991 136 Size: 107 122 71 631 605 2319 1525 Fragments arranged by size: 2319 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5375 5375 Size: 5380 BlpI GC'TnA_GC Cuts at: 2200 2797 2200 Size: 597 4783 Bme1580I G_kGCm'C Cuts at: 1790 3441 4597 1790 Size: 1651 1156 2573 Fragments arranged by size: 2573 1651 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5380 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3111 4069 442 Size: 416 53 83 399 24 1694 958 1753 Fragments arranged by size: 1753 1694 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2108 1056 Size: 1052 4328 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2203 4040 1239 Size: 964 1837 2579 Fragments arranged by size: 2579 1837 964 Bpu10I CC'TnA_GC Cuts at: 2024 2024 Size: 5380 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3505 4370 4668 3505 Size: 865 298 4217 Fragments arranged by size: 4217 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2014 4043 882 Size: 617 515 2029 2219 Fragments arranged by size: 2219 2029 617 515 BsaAI yAC'GTr Cuts at: 493 1204 3171 493 Size: 711 1967 2702 Fragments arranged by size: 2702 1967 711 BsaBI GATnn'nnATC Cuts at: 5374 5374 Size: 5380 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3187 3569 4204 275 Size: 53 83 186 936 1654 382 635 1451 Fragments arranged by size: 1654 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3813 4211 4485 4632 5043 3813 Size: 398 274 147 411 4150 Fragments arranged by size: 4150 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2586 2616 1178 Size: 30 165 30 1183 30 3942 Fragments arranged by size: 3942 1183 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5380 BseYI C'CCAG_C Cuts at: 2380 2662 4583 5079 2380 Size: 282 1921 496 2681 Fragments arranged by size: 2681 1921 496 282 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2252 1163 Size: 573 516 4291 Fragments arranged by size: 4291 573 516 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2830 3591 4619 1540 Size: 275 186 829 761 1028 2301 Fragments arranged by size: 2301 1028 829 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3441 3526 4597 3441 Size: 85 1071 4224 Fragments arranged by size: 4224 1071 85 BsmI GAATG_Cn' Cuts at: 2891 2984 2891 Size: 93 5287 BsmBI CGTCTCn'nnnn_ Cuts at: 2441 2441 Size: 5380 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2699 3441 3526 4597 1391 Size: 399 909 742 85 1071 2174 Fragments arranged by size: 2174 1071 909 742 399 85 BspEI T'CCGG_A Cuts at: 5043 5043 Size: 5380 BspHI T'CATG_A Cuts at: 2529 3269 4999 2529 Size: 740 1730 2910 Fragments arranged by size: 2910 1730 740 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2218 2426 2735 844 Size: 1374 208 309 3489 Fragments arranged by size: 3489 1374 309 208 BsrBI CCG'CTC Cuts at: 1394 1823 3267 1394 Size: 429 1444 3507 Fragments arranged by size: 3507 1444 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3875 4049 65 Size: 1183 2627 174 1396 Fragments arranged by size: 2627 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4024 4211 1700 Size: 110 2214 187 2869 Fragments arranged by size: 2869 2214 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5380 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5380 BssSI C'ACGA_G Cuts at: 3440 4452 3440 Size: 1012 4368 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5380 BstBI TT'CG_AA Cuts at: 4184 4184 Size: 5380 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5380 BstXI CCAn_nnnn'nTGG Cuts at: 2554 2554 Size: 5380 BstYI r'GATC_y Cuts at: 1972 2710 3077 3462 3479 4931 5017 5029 5375 Size: 738 367 385 17 1452 86 12 346 Cuts at: 5375 1972 Size: 1977 Fragments arranged by size: 1977 1452 738 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2157 513 Size: 552 939 153 3736 Fragments arranged by size: 3736 939 552 153 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4641 Fragments arranged by size: 4641 683 56 BtsI GCAGTG_nn' Cuts at: 2175 2896 3690 3710 2175 Size: 721 794 20 3845 Fragments arranged by size: 3845 794 721 20 ClaI AT'CG_AT Cuts at: 1554 2097 1554 Size: 543 4837 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2098 2133 529 Size: 35 1534 35 3776 Fragments arranged by size: 3776 1534 35 35 DraI TTT'AAA Cuts at: 2499 2684 2499 Size: 185 5195 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2075 4387 809 Size: 507 759 2312 1802 Fragments arranged by size: 2312 1802 759 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2827 3716 5345 Size: 54 1425 50 275 18 997 889 1629 Cuts at: 5345 8 Size: 43 Fragments arranged by size: 1629 1425 997 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2827 1537 Size: 275 1015 4090 Fragments arranged by size: 4090 1015 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3310 1353 Size: 1957 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3967 4341 4487 387 Size: 1064 138 2378 374 146 1280 Fragments arranged by size: 2378 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2203 3443 4040 4827 5095 1239 Size: 393 571 1240 597 787 268 1524 Fragments arranged by size: 1524 1240 787 597 571 393 268 EcoRI G'AATT_C Cuts at: 2689 3067 2689 Size: 378 5002 FspI TGC'GCA Cuts at: 3886 3886 Size: 5380 HaeII r_GCGC'y Cuts at: 1678 2200 4527 1678 Size: 522 2327 2531 Fragments arranged by size: 2531 2327 522 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 4021 4053 4095 Size: 32 135 32 548 32 1869 32 42 Cuts at: 4095 4127 1373 Size: 32 2626 Fragments arranged by size: 2626 1869 548 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2068 2724 2970 669 Size: 988 281 130 656 246 3079 Fragments arranged by size: 3079 988 656 281 246 130 HindIII A'AGCT_T Cuts at: 748 2030 748 Size: 1282 4098 HpaI GTT'AAC Cuts at: 2970 2970 Size: 5380 KpnI G_GTAC'C Cuts at: 2705 2705 Size: 5380 MfeI C'AATT_G Cuts at: 2979 2979 Size: 5380 MluI A'CGCG_T Cuts at: 5256 5256 Size: 5380 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4494 4678 1305 Size: 3189 184 2007 Fragments arranged by size: 3189 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3558 Fragments arranged by size: 3558 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 3338 3697 3856 518 Size: 648 1386 786 359 159 2042 Fragments arranged by size: 2042 1386 786 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2462 2796 3473 4621 4866 1606 Size: 856 334 677 1148 245 2120 Fragments arranged by size: 2120 1148 856 677 334 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2157 513 Size: 552 939 153 3736 Fragments arranged by size: 3736 939 552 153 NdeI CA'TA_TG Cuts at: 387 387 Size: 5380 NheI G'CTAG_C Cuts at: 1052 2104 1052 Size: 1052 4328 NotI GC'GGCC_GC Cuts at: 2827 2827 Size: 5380 NspI r_CATG'y Cuts at: 1132 1229 2360 2738 4283 1132 Size: 97 1131 378 1545 2229 Fragments arranged by size: 2229 1545 1131 378 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5380 PciI A'CATG_T Cuts at: 4279 4279 Size: 5380 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5380 PmeI GTTT'AAAC Cuts at: 2684 2684 Size: 5380 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3449 3481 4962 4994 3449 Size: 32 1481 32 3835 Fragments arranged by size: 3835 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5380 PsiI TTA'TAA Cuts at: 2950 2950 Size: 5380 PstI C_TGCA'G Cuts at: 830 2732 830 Size: 1902 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5380 PvuII CAG'CTG Cuts at: 2462 2462 Size: 5380 SalI G'TCGA_C Cuts at: 1936 2722 1936 Size: 786 4594 SbfI CC_TGCA'GG Cuts at: 2732 2732 Size: 5380 ScaI AGT'ACT Cuts at: 3628 5340 3628 Size: 1712 3668 SfcI C'TryA_G Cuts at: 826 1045 2297 2728 3863 4544 4735 826 Size: 219 1252 431 1135 681 191 1471 Fragments arranged by size: 1471 1252 1135 681 431 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5380 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5380 SmaI CCC'GGG Cuts at: 1872 2707 1872 Size: 835 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3484 4385 4647 820 Size: 197 926 1541 901 262 1553 Fragments arranged by size: 1553 1541 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3171 493 Size: 2678 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5380 SphI G_CATG'C Cuts at: 1132 2738 1132 Size: 1606 3774 SspI AAT'ATT Cuts at: 5 52 3304 5 Size: 47 3252 2081 Fragments arranged by size: 3252 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5380 StyI C'CwwG_G Cuts at: 513 1065 2004 2157 2819 513 Size: 552 939 153 662 3074 Fragments arranged by size: 3074 939 662 552 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3407 3424 3577 3762 4207 325 Size: 1255 1827 17 153 185 445 1498 Fragments arranged by size: 1827 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3626 5338 96 Size: 274 80 33 51 1443 1649 1712 138 Fragments arranged by size: 1712 1649 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2586 2618 5340 5372 2586 Size: 32 2722 32 2594 Fragments arranged by size: 2722 2594 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5380 XbaI T'CTAG_A Cuts at: 2716 2716 Size: 5380 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5380 XmaI C'CCGG_G Cuts at: 1870 2705 1870 Size: 835 4545 XmnI GAAnn'nnTTC Cuts at: 2033 2508 3509 2033 Size: 475 1001 3904 Fragments arranged by size: 3904 1001 475 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3188 276 Size: 53 83 186 936 1654 2468 Fragments arranged by size: 2468 1654 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI