(Linear) (Six-base) MAPSORT of: KIEE6624.seq  Check: 7197  from: 1  to: 675



KIEE6624.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     213     675 
   Size:        213     462
 
AfeI AGC'GCT

Cuts at:      0     193     675 
   Size:        193     482
 
AhdI GACnn_n'nnGTC

Cuts at:      0     129     675 
   Size:        129     546
 
AlwNI CAG_nnn'CTG

Cuts at:      0      46     675 
   Size:         46     629
 
ApoI r'AATT_y

Cuts at:      0     406     502     675 
   Size:        406      96     173

  Fragments arranged by size: 

                 406     173      96
 
BanI G'GyrC_C

Cuts at:      0     538     675 
   Size:        538     137
 
BbvCI CC'TCA_GC

Cuts at:      0      19     675 
   Size:         19     656
 
BclI T'GATC_A

Cuts at:      0     599     675 
   Size:        599      76
 
BlpI GC'TnA_GC

Cuts at:      0     195     675 
   Size:        195     480
 
BmtI G_CTAG'C

Cuts at:      0     103     675 
   Size:        103     572
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     675 
   Size:        198     477
 
Bpu10I CC'TnA_GC

Cuts at:      0      19     675 
   Size:         19     656
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       9     675 
   Size:          9     666
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     581     611     675 
   Size:        581      30      64

  Fragments arranged by size: 

                 581      64      30
 
BseYI C'CCAG_C

Cuts at:      0     375     657     675 
   Size:        375     282      18

  Fragments arranged by size: 

                 375     282      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     675 
   Size:        247     428
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     436     675 
   Size:        436     239
 
BspHI T'CATG_A

Cuts at:      0     524     675 
   Size:        524     151
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     213     421     675 
   Size:        213     208     254

  Fragments arranged by size: 

                 254     213     208
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     549     675 
   Size:        549     126
 
BtgI C'CryG_G

Cuts at:      0     152     675 
   Size:        152     523
 
BtsI GCAGTG_nn'

Cuts at:      0     170     675 
   Size:        170     505
 
ClaI AT'CG_AT

Cuts at:      0      92     675 
   Size:         92     583
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      93     128     675 
   Size:         93      35     547

  Fragments arranged by size: 

                 547      93      35
 
DraI TTT'AAA

Cuts at:      0     494     675 
   Size:        494     181
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      70     675 
   Size:         70     605
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     675 
   Size:        198     477
 
HaeII r_GCGC'y

Cuts at:      0     195     675 
   Size:        195     480
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     115     147     675 
   Size:        115      32     528

  Fragments arranged by size: 

                 528     115      32
 
HincII GTy'rAC

Cuts at:      0      63     675 
   Size:         63     612
 
HindIII A'AGCT_T

Cuts at:      0      25     675 
   Size:         25     650
 
MslI CAynn'nnrTG

Cuts at:      0     547     675 
   Size:        547     128
 
MspA1I CmG'CkG

Cuts at:      0     457     675 
   Size:        457     218
 
NcoI C'CATG_G

Cuts at:      0     152     675 
   Size:        152     523
 
NheI G'CTAG_C

Cuts at:      0      99     675 
   Size:         99     576
 
NspI r_CATG'y

Cuts at:      0     355     675 
   Size:        355     320
 
PvuII CAG'CTG

Cuts at:      0     457     675 
   Size:        457     218
 
SfcI C'TryA_G

Cuts at:      0     292     675 
   Size:        292     383
 
StyI C'CwwG_G

Cuts at:      0     152     675 
   Size:        152     523
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     581     613     675 
   Size:        581      32      62

  Fragments arranged by size: 

                 581      62      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      28     503     675 
   Size:         28     475     172

  Fragments arranged by size: 

                 475     172      28
 

 Enzymes that do cut:

     AarI     AfeI     AhdI    AlwNI     ApoI     BanI    BbvCI     BclI
     BlpI     BmtI     BpmI   Bpu10I     BsaI    BsaXI    BseYI     BsgI
    BsmBI    BspHI    BspMI    BstXI     BtgI     BtsI     ClaI    CspCI
     DraI     DrdI  Eco57MI    HaeII    Hin4I   HincII  HindIII     MslI
   MspA1I     NcoI     NheI     NspI    PvuII     SfcI     StyI     TstI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI     BcgI
    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BplI
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
    BtgZI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI