Restriction map for
pFN21AE5185
##################################
# NO CUT ENZYMES ( 207 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII ItaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MluNI MnlI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI TseI Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AciI 135 GCGG CCGC(-3/-1)
Alw44I 171 GTGCAC G^TGCAC
ApaLI 171 GTGCAC G^TGCAC
AspHI 171 GTGCAC G[AT]GC[AT]^C
BamHI 105 GGATCC G^GATCC
BlpI 348 GCTAAGC GC^T[ACGT]AGC
BmyI 171 GTGCAC G[AGT]GC[ACT]^C
BpmI 316 CTGGAG CTGGAG(16/14)
Bpu1102I 348 GCTAAGC GC^T[ACGT]AGC
BsiHKAI 171 GTGCAC G[AT]GC[AT]^C
BsmFI 55 GGGAC GGGAC(10/14)
Bsp1286I 171 GTGCAC G[AGT]GC[ACT]^C
BssSI 215 CACGAG CACGAG(-5/-1)
BstF5I 17 CATCC GGATG(2/0)
CelII 348 GCTAAGC GC^T[ACGT]AGC
EarI 378 CTCTTC CTCTTC(1/4)
FokI 17 CATCC GGATG(9/13)
HinfI 321 GAGTC G^A[ACGT]TC
HphI 48 GGTGA GGTGA(8/7)
Ksp632I 378 CTCTTC CTCTTC(1/4)
MwoI 151 GCTCACGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 105 GGATCC GG[ACGT]^[ACGT]CC
NspI 225 GCATGT [GA]CATG^[CT]
PleI 321 GAGTC GAGTC(4/5)
SfaNI 157 GATGC GCATC(5/9)
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AluI 201 AGCT AG^CT
347 AGCT
BsiYI 280 CCAGTGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
295 CCCTGTGCTGG
BslI 280 CCAGTGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
295 CCCTGTGCTGG
BsrI 240 ACTGG ACTGG(1/-1)
280 CCAGT
BssKI 92 CCAGG ^CC[ACGT]GG
315 CCTGG
BstNI 92 CCAGG CC^[AT]GG
315 CCTGG
BstYI 105 GGATCC [GA]^GATC[CT]
276 AGATCC
EcoRII 92 CCAGG ^CC[AT]GG
315 CCTGG
MaeIII 88 GTGAC ^GT[ACGT]AC
283 GTGAC
MboII 364 GAAGA GAAGA(8/7)
379 TCTTC
MflI 105 GGATCC [GA]^GATC[CT]
276 AGATCC
MseI 118 TTAA T^TAA
128 TTAA
MvaI 92 CCAGG CC^[AT]GG
315 CCTGG
ScrFI 92 CCAGG CC^[ACGT]GG
315 CCTGG
Tru9I 118 TTAA T^TAA
128 TTAA
Tsp45I 88 GTGAC ^GT[GC]AC
283 GTGAC
TspRI 169 CAGTG CA[GC]TG[ACGT][ACGT]^
281 CAGTG
XcmI 29 CCAGATGGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
92 CCAGGCTTGTTCTGG
XhoII 105 GGATCC [GA]^GATC[CT]
276 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
AlwI DdeI DpnII MboI NdeII
NlaIII Sau3AI