(Circular) (Six-base) MAPSORT of: pFN21AE6669.seq Check: 4943 from: 1 to: 5593 pFN21AE6669 5593 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3403 278 Size: 53 83 186 936 1867 2468 Fragments arranged by size: 2468 1867 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2936 1937 Size: 999 4594 Acc65I G'GTAC_C Cuts at: 2914 2914 Size: 5593 AclI AA'CG_TT Cuts at: 1563 3720 4093 1563 Size: 2157 373 3063 Fragments arranged by size: 3063 2157 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3656 5040 5308 1632 Size: 2024 1384 268 1917 Fragments arranged by size: 2024 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5396 AflIII A'CryG_T Cuts at: 1203 4492 5469 1203 Size: 3289 977 1327 Fragments arranged by size: 3289 1327 977 AgeI A'CCGG_T Cuts at: 4424 4424 Size: 5593 AhdI GACnn_n'nnGTC Cuts at: 2613 4322 2613 Size: 1709 3884 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5593 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5593 AlwNI CAG_nnn'CTG Cuts at: 2332 3336 4908 2332 Size: 1004 1572 3017 Fragments arranged by size: 3017 1572 1004 ApaLI G'TGCA_C Cuts at: 3650 4806 3650 Size: 1156 4437 ApoI r'AATT_y Cuts at: 1493 2399 2902 3128 3280 5389 1493 Size: 906 503 226 152 2109 1697 Fragments arranged by size: 2109 1697 906 503 226 152 AseI AT'TA_AT Cuts at: 160 1032 4147 160 Size: 872 3115 1606 Fragments arranged by size: 3115 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5593 AvaI C'yCGr_G Cuts at: 1870 1943 2018 2147 2918 1870 Size: 73 75 129 771 4545 Fragments arranged by size: 4545 771 129 75 73 BamHI G'GATC_C Cuts at: 2923 3290 2923 Size: 367 5226 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2914 4369 618 Size: 325 217 627 1127 1455 1842 Fragments arranged by size: 1842 1455 1127 627 325 217 BanII G_rGCy'C Cuts at: 1391 2019 2148 2237 2912 1391 Size: 628 129 89 675 4072 Fragments arranged by size: 4072 675 628 129 89 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4622 BbvCI CC'TCA_GC Cuts at: 2104 2104 Size: 5593 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3784 3818 1903 Size: 34 1847 34 3678 Fragments arranged by size: 3678 1847 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3485 4695 3485 Size: 1210 4383 BclI T'GATC_A Cuts at: 1548 1548 Size: 5593 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2061 4204 136 Size: 107 122 71 631 605 389 2143 1525 Fragments arranged by size: 2143 1525 631 605 389 122 107 71 BglII A'GATC_T Cuts at: 5588 5588 Size: 5593 BlpI GC'TnA_GC Cuts at: 2346 3010 2346 Size: 664 4929 Bme1580I G_kGCm'C Cuts at: 1790 3654 4810 1790 Size: 1864 1156 2573 Fragments arranged by size: 2573 1864 1156 BmgBI CAC'GTC Cuts at: 1318 2284 1318 Size: 966 4627 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3324 4282 442 Size: 416 53 83 399 24 1907 958 1753 Fragments arranged by size: 1907 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5593 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2221 2639 4253 1239 Size: 982 418 1614 2579 Fragments arranged by size: 2579 1614 982 418 Bpu10I CC'TnA_GC Cuts at: 2104 2325 2104 Size: 221 5372 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3718 4583 4881 3718 Size: 865 298 4430 Fragments arranged by size: 4430 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2539 4256 882 Size: 617 1040 1717 2219 Fragments arranged by size: 2219 1717 1040 617 BsaAI yAC'GTr Cuts at: 493 1204 3384 493 Size: 711 2180 2702 Fragments arranged by size: 2702 2180 711 BsaBI GATnn'nnATC Cuts at: 5587 5587 Size: 5593 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3400 3782 4417 275 Size: 53 83 186 936 1867 382 635 1451 Fragments arranged by size: 1867 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4026 4424 4698 4845 5256 4026 Size: 398 274 147 411 4363 Fragments arranged by size: 4363 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5368 Fragments arranged by size: 5368 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2387 2444 1186 Size: 1201 57 4335 Fragments arranged by size: 4335 1201 57 BseYI C'CCAG_C Cuts at: 4796 5292 4796 Size: 496 5097 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2825 1163 Size: 573 1089 3931 Fragments arranged by size: 3931 1089 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2322 3043 3804 4832 1540 Size: 275 186 321 721 761 1028 2301 Fragments arranged by size: 2301 1028 761 721 321 275 186 BsiHKAI G_wGCw'C Cuts at: 2148 2237 3654 3739 4810 2148 Size: 89 1417 85 1071 2931 Fragments arranged by size: 2931 1417 1071 89 85 BsmI GAATG_Cn' Cuts at: 2014 2501 3104 3197 2014 Size: 487 603 93 4410 Fragments arranged by size: 4410 603 487 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2148 2237 2912 3654 3739 4810 Size: 399 229 129 89 675 742 85 1071 Cuts at: 4810 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 675 399 229 129 89 85 BspEI T'CCGG_A Cuts at: 5256 5256 Size: 5593 BspHI T'CATG_A Cuts at: 3482 5212 3482 Size: 1730 3863 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2445 2948 844 Size: 1601 503 3489 Fragments arranged by size: 3489 1601 503 BsrBI CCG'CTC Cuts at: 1394 1823 2324 2485 3480 1394 Size: 429 501 161 995 3507 Fragments arranged by size: 3507 995 501 429 161 BsrDI GCAATG_nn' Cuts at: 65 1248 4088 4262 65 Size: 1183 2840 174 1396 Fragments arranged by size: 2840 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 4237 4424 1700 Size: 110 250 2177 187 2869 Fragments arranged by size: 2869 2177 250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5593 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5593 BssSI C'ACGA_G Cuts at: 2369 3653 4665 2369 Size: 1284 1012 3297 Fragments arranged by size: 3297 1284 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5593 BstBI TT'CG_AA Cuts at: 4397 4397 Size: 5593 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5593 BstXI CCAn_nnnn'nTGG Cuts at: 2646 2646 Size: 5593 BstYI r'GATC_y Cuts at: 1972 2865 2923 3290 3675 3692 5144 5230 5242 Size: 893 58 367 385 17 1452 86 12 Cuts at: 5242 5588 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 893 385 367 346 86 58 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5041 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4854 Fragments arranged by size: 4854 683 56 BtsI GCAGTG_nn' Cuts at: 2233 2842 3109 3903 3923 2233 Size: 609 267 794 20 3903 Fragments arranged by size: 3903 794 609 267 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5593 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5558 DraI TTT'AAA Cuts at: 2897 2897 Size: 5593 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4600 809 Size: 507 3284 1802 Fragments arranged by size: 3284 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 2319 3040 Size: 54 1425 50 275 18 223 266 721 Cuts at: 3040 3929 5558 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 721 275 266 223 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2319 3040 1537 Size: 275 507 721 4090 Fragments arranged by size: 4090 721 507 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2462 2475 2571 2856 3523 1353 Size: 1109 13 96 285 667 3423 Fragments arranged by size: 3423 1109 667 285 96 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4180 4554 4700 387 Size: 1064 138 2591 374 146 1280 Fragments arranged by size: 2591 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2146 2235 2146 Size: 89 5504 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2221 2639 3656 4253 5040 5308 1239 Size: 393 589 418 1017 597 787 268 1524 Fragments arranged by size: 1524 1017 787 597 589 418 393 268 EcoO109I rG'GnC_Cy Cuts at: 2663 2712 2663 Size: 49 5544 EcoRI G'AATT_C Cuts at: 2399 2902 3280 2399 Size: 503 378 4712 Fragments arranged by size: 4712 503 378 FspI TGC'GCA Cuts at: 4099 4099 Size: 5593 HaeII r_GCGC'y Cuts at: 1678 4740 1678 Size: 3062 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2599 2631 4234 4266 4308 Size: 32 135 32 1027 32 1603 32 42 Cuts at: 4308 4340 1373 Size: 32 2626 Fragments arranged by size: 2626 1603 1027 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2937 3183 669 Size: 988 281 999 246 3079 Fragments arranged by size: 3079 999 988 281 246 HindIII A'AGCT_T Cuts at: 748 2692 748 Size: 1944 3649 HpaI GTT'AAC Cuts at: 3183 3183 Size: 5593 KpnI G_GTAC'C Cuts at: 2918 2918 Size: 5593 MfeI C'AATT_G Cuts at: 3192 3192 Size: 5593 MluI A'CGCG_T Cuts at: 5469 5469 Size: 5593 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2454 4707 4891 1305 Size: 1149 2253 184 2007 Fragments arranged by size: 2253 2007 1149 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3771 Fragments arranged by size: 3771 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2644 3551 3910 4069 518 Size: 648 1478 907 359 159 2042 Fragments arranged by size: 2042 1478 907 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2465 2508 3009 3686 4834 5079 1606 Size: 859 43 501 677 1148 245 2120 Fragments arranged by size: 2120 1148 859 677 501 245 43 NaeI GCC'GGC Cuts at: 2062 2062 Size: 5593 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5041 NdeI CA'TA_TG Cuts at: 387 387 Size: 5593 NgoMIV G'CCGG_C Cuts at: 2060 2060 Size: 5593 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5593 NotI GC'GGCC_GC Cuts at: 2319 3040 2319 Size: 721 4872 NspI r_CATG'y Cuts at: 1132 1229 2381 2951 4496 1132 Size: 97 1152 570 1545 2229 Fragments arranged by size: 2229 1545 1152 570 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5593 PciI A'CATG_T Cuts at: 4492 4492 Size: 5593 PflMI CCAn_nnn'nTGG Cuts at: 1108 2206 1108 Size: 1098 4495 PmeI GTTT'AAAC Cuts at: 2897 2897 Size: 5593 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3662 3694 5175 5207 3662 Size: 32 1481 32 4048 Fragments arranged by size: 4048 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2663 2712 2663 Size: 49 5544 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5593 PsiI TTA'TAA Cuts at: 3163 3163 Size: 5593 PstI C_TGCA'G Cuts at: 830 2228 2945 830 Size: 1398 717 3478 Fragments arranged by size: 3478 1398 717 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5593 PvuII CAG'CTG Cuts at: 2465 2465 Size: 5593 SacI G_AGCT'C Cuts at: 2148 2237 2148 Size: 89 5504 SalI G'TCGA_C Cuts at: 1936 2935 1936 Size: 999 4594 SapI GCTCTTCn'nnn_ Cuts at: 2475 2571 2475 Size: 96 5497 SbfI CC_TGCA'GG Cuts at: 2945 2945 Size: 5593 ScaI AGT'ACT Cuts at: 3841 5553 3841 Size: 1712 3881 SexAI A'CCwGG_T Cuts at: 2665 2665 Size: 5593 SfcI C'TryA_G Cuts at: 826 1045 2224 2941 4076 4757 4948 826 Size: 219 1179 717 1135 681 191 1471 Fragments arranged by size: 1471 1179 1135 717 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5593 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5593 SmaI CCC'GGG Cuts at: 1872 2920 1872 Size: 1048 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3697 4598 4860 820 Size: 197 926 1754 901 262 1553 Fragments arranged by size: 1754 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3384 493 Size: 2891 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5593 SphI G_CATG'C Cuts at: 1132 2951 1132 Size: 1819 3774 SspI AAT'ATT Cuts at: 5 52 2876 3517 5 Size: 47 2824 641 2081 Fragments arranged by size: 2824 2081 641 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5593 StyI C'CwwG_G Cuts at: 513 1065 2715 3032 513 Size: 552 1650 317 3074 Fragments arranged by size: 3074 1650 552 317 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3620 3637 3790 3975 4420 325 Size: 1255 2040 17 153 185 445 1498 Fragments arranged by size: 2040 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3839 5551 96 Size: 274 80 33 51 1443 1862 1712 138 Fragments arranged by size: 1862 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5553 5585 5553 Size: 32 5561 Tth111I GACn'n_nGTC Cuts at: 1316 2308 2667 1316 Size: 992 359 4242 Fragments arranged by size: 4242 992 359 XbaI T'CTAG_A Cuts at: 2929 2929 Size: 5593 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2203 2705 2203 Size: 502 5091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5593 XmaI C'CCGG_G Cuts at: 1870 2918 1870 Size: 1048 4545 XmnI GAAnn'nnTTC Cuts at: 3722 3722 Size: 5593 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3401 276 Size: 53 83 186 936 1867 2468 Fragments arranged by size: 2468 1867 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI