################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AspEI AspI AvaII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DraII DraIII DrdI Eam1105I Eco47III EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HgaI HindIII MaeII MamI MfeI MflI MluI MluNI MroI MscI MunI NcoI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SalI SbfI ScaI SexAI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI Tth111I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 673 GGTACC G^GTACC AcyI 43 GGCGCC G[GA]^CG[CT]C AocI 50 CCTCAGG CC^T[ACGT]AGG Asp718I 673 GGTACC G^GTACC AspHI 655 GAGCTC G[AT]GC[AT]^C AviII 149 TGCGCA TGC^GCA BbeI 43 GGCGCC GGCGC^C BciVI 1062 GGATAC GTATCC(6/5) BsaHI 43 GGCGCC G[GA]^CG[CT]C BseRI 33 CTCCTC GAGGAG(10/8) BsiEI 94 CGGCCG CG[GA][CT]^CG BsiHKAI 655 GAGCTC G[AT]GC[AT]^C BsmAI 385 GAGAC GTCTC(1/5) BsrBI 81 GAGCGG CCGCTC(-3/-3) BsrDI 794 GCAATG GCAATG(2/0) BsrFI 92 GCCGGC [GA]^CCGG[CT] BsrI 444 CCAGT ACTGG(1/-1) BssSI 536 CTCGTG CACGAG(-5/-1) Bsu36I 50 CCTCAGG CC^T[ACGT]AGG Cfr10I 92 GCCGGC [GA]^CCGG[CT] EaeI 94 CGGCCG [CT]^GGCC[GA] EagI 94 CGGCCG C^GGCCG EclXI 94 CGGCCG C^GGCCG Eco52I 94 CGGCCG C^GGCCG Eco81I 50 CCTCAGG CC^T[ACGT]AGG EcoNI 784 CCTTTCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 355 CCTTGG C^C[AT][AT]GG FspI 149 TGCGCA TGC^GCA HaeII 43 GGCGCC [GA]GCGC^[CT] Hin1I 43 GGCGCC G[GA]^CG[CT]C HpaI 753 GTTAAC GTT^AAC KasI 43 GGCGCC G^GCGCC KpnI 673 GGTACC GGTAC^C KspI 117 CCGCGG CCGC^GG NaeI 92 GCCGGC GCC^GGC NarI 43 GGCGCC GG^CGCC NdeI 758 CATATG CA^TATG NgoMIV 92 GCCGGC G^CCGGC PleI 874 GAGTC GAGTC(4/5) SacI 655 GAGCTC GAGCT^C SacII 117 CCGCGG CCGC^GG SapI 652 GAAGAGC GCTCTTC(1/4) SfaNI 391 GCATC GCATC(5/9) SfcI 174 CTACAG C^T[GA][CT]AG SfoI 43 GGCGCC GGC^GCC SmaI 186 CCCGGG CCC^GGG StyI 355 CCTTGG C^C[AT][AT]GG SwaI 1191 ATTTAAAT ATTT^AAAT TaqI 924 TCGA T^CGA Tsp45I 602 GTGAC ^GT[GC]AC TthHB8I 924 TCGA T^CGA XmaCI 186 CCCGGG C^CCGGG XmaI 186 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 118 CGCG CG^CG 139 CGCG AfaI 331 GTAC GT^AC 674 GTAC AluI 197 AGCT AG^CT 656 AGCT AlwI 3 GATCC GGATC(4/5) 712 GATCC AseI 364 ATTAAT AT^TAAT 1054 ATTAAT AsnI 364 ATTAAT AT^TAAT 1054 ATTAAT Asp700I 310 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 514 GAAGGTGTTC AvaI 57 CTCGGG C^[CT]CG[GA]G 186 CCCGGG BanI 43 GGCGCC G^G[CT][GA]CC 673 GGTACC BanII 157 GAGCCC G[GA]GC[CT]^C 655 GAGCTC BglI 62 GCCGGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 87 GCCGTGCCGGC BsoBI 57 CTCGGG C^[CT]CG[GA]G 186 CCCGGG BspHI 272 TCATGA T^CATGA 318 TCATGA BstF5I 305 GGATG GGATG(2/0) 995 GGATG BstUI 118 CGCG CG^CG 139 CGCG DraI 472 TTTAAA TTT^AAA 1192 TTTAAA DsaI 117 CCGCGG C^C[GA][CT]GG 143 CCGTGG EarI 652 GAAGAG CTCTTC(1/4) 1102 GAAGAG FokI 305 GGATG GGATG(9/13) 995 GGATG Ksp632I 652 GAAGAG CTCTTC(1/4) 1102 GAAGAG MslI 532 CACACTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 744 CATTGGAGTG MspA1I 24 CAGCGG C[AC]G^C[GT]G 117 CCGCGG MvnI 118 CGCG CG^CG 139 CGCG PshBI 364 ATTAAT AT^TAAT 1054 ATTAAT RcaI 272 TCATGA T^CATGA 318 TCATGA RsaI 331 GTAC GT^AC 674 GTAC SfiI 61 GGCCGGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 86 GGCCGTGCCGGCC SspI 571 AATATT AAT^ATT 636 AATATT XmnI 310 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 514 GAAGGTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AcsI ApoI BbvI BcnI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp509I TspRI