(Circular) (Six-base) MAPSORT of: pFN21AE0718.seq Check: 6021 from: 1 to: 5947 pFN21AE0718 5947 bp With 182 enzymes: SgfI * March 28, 2012 12:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3757 278 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3290 1937 Size: 1353 4594 Acc65I G'GTAC_C Cuts at: 2678 3268 2678 Size: 590 5357 AclI AA'CG_TT Cuts at: 1563 4074 4447 1563 Size: 2511 373 3063 Fragments arranged by size: 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2943 2961 4010 5394 5662 1632 Size: 1311 18 1049 1384 268 1917 Fragments arranged by size: 1917 1384 1311 1049 268 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5750 AflIII A'CryG_T Cuts at: 1203 4846 5823 1203 Size: 3643 977 1327 Fragments arranged by size: 3643 1327 977 AgeI A'CCGG_T Cuts at: 4778 4778 Size: 5947 AhdI GACnn_n'nnGTC Cuts at: 4676 4676 Size: 5947 AleI CACnn'nnGTG Cuts at: 1166 2541 1166 Size: 1375 4572 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5947 AlwNI CAG_nnn'CTG Cuts at: 3690 5262 3690 Size: 1572 4375 ApaLI G'TGCA_C Cuts at: 4004 5160 4004 Size: 1156 4791 ApoI r'AATT_y Cuts at: 1493 2216 2261 2703 2839 2861 3200 3225 3236 Size: 723 45 442 136 22 339 25 11 Cuts at: 3236 3256 3482 3634 5743 1493 Size: 20 226 152 2109 1697 Fragments arranged by size: 2109 1697 723 442 339 226 152 136 45 25 22 20 11 AseI AT'TA_AT Cuts at: 160 1032 2370 3060 4501 160 Size: 872 1338 690 1441 1606 Fragments arranged by size: 1606 1441 1338 872 690 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2191 3272 1870 Size: 73 119 129 1081 4545 Fragments arranged by size: 4545 1081 129 119 73 BamHI G'GATC_C Cuts at: 3277 3644 3277 Size: 367 5580 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2678 3268 4723 618 Size: 325 217 627 261 630 590 1455 1842 Fragments arranged by size: 1842 1455 630 627 590 325 261 217 BanII G_rGCy'C Cuts at: 1391 2166 2664 3266 1391 Size: 775 498 602 4072 Fragments arranged by size: 4072 775 602 498 BbeI G_GCGC'C Cuts at: 2052 2052 Size: 5947 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4976 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4138 4172 1903 Size: 34 2201 34 3678 Fragments arranged by size: 3678 2201 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3061 3839 5049 3061 Size: 778 1210 3959 Fragments arranged by size: 3959 1210 778 BclI T'GATC_A Cuts at: 1548 1548 Size: 5947 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2073 2098 4558 Size: 107 122 71 631 605 401 25 2460 Cuts at: 4558 136 Size: 1525 Fragments arranged by size: 2460 1525 631 605 401 122 107 71 25 BglII A'GATC_T Cuts at: 5942 5942 Size: 5947 BlpI GC'TnA_GC Cuts at: 3364 3364 Size: 5947 Bme1580I G_kGCm'C Cuts at: 1790 2192 4008 5164 1790 Size: 402 1816 1156 2573 Fragments arranged by size: 2573 1816 1156 402 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5947 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3678 4636 442 Size: 416 53 83 399 24 2261 958 1753 Fragments arranged by size: 2261 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4607 1239 Size: 3368 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4072 4937 5235 4072 Size: 865 298 4784 Fragments arranged by size: 4784 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4610 882 Size: 617 3111 2219 Fragments arranged by size: 3111 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3738 493 Size: 711 2534 2702 Fragments arranged by size: 2702 2534 711 BsaBI GATnn'nnATC Cuts at: 5941 5941 Size: 5947 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 3754 4136 4771 Size: 53 83 186 936 516 1705 382 635 Cuts at: 4771 275 Size: 1451 Fragments arranged by size: 1705 1451 936 635 516 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4380 4778 5052 5199 5610 4380 Size: 398 274 147 411 4717 Fragments arranged by size: 4717 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5722 Fragments arranged by size: 5722 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 1186 Size: 843 5104 BseYI C'CCAG_C Cuts at: 2203 2429 5150 5646 2203 Size: 226 2721 496 2504 Fragments arranged by size: 2721 2504 496 226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5374 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2102 3397 4158 5186 1540 Size: 275 186 101 1295 761 1028 2301 Fragments arranged by size: 2301 1295 1028 761 275 186 101 BsiHKAI G_wGCw'C Cuts at: 2664 4008 4093 5164 2664 Size: 1344 85 1071 3447 Fragments arranged by size: 3447 1344 1071 85 BsmI GAATG_Cn' Cuts at: 3458 3551 3458 Size: 93 5854 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2192 2664 3266 4008 4093 5164 Size: 399 376 26 472 602 742 85 1071 Cuts at: 5164 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 602 472 399 376 85 26 BspEI T'CCGG_A Cuts at: 5610 5610 Size: 5947 BspHI T'CATG_A Cuts at: 2277 2323 3836 5566 2277 Size: 46 1513 1730 2658 Fragments arranged by size: 2658 1730 1513 46 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3302 844 Size: 2458 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2088 3834 1394 Size: 429 265 1746 3507 Fragments arranged by size: 3507 1746 429 265 BsrDI GCAATG_nn' Cuts at: 65 1248 2806 4442 4616 65 Size: 1183 1558 1636 174 1396 Fragments arranged by size: 1636 1558 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2097 4591 4778 1700 Size: 110 287 2494 187 2869 Fragments arranged by size: 2869 2494 287 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5947 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5947 BssSI C'ACGA_G Cuts at: 2541 4007 5019 2541 Size: 1466 1012 3469 Fragments arranged by size: 3469 1466 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5947 BstBI TT'CG_AA Cuts at: 4751 4751 Size: 5947 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5947 BstYI r'GATC_y Cuts at: 1972 3277 3644 4029 4046 5498 5584 5596 5942 Size: 1305 367 385 17 1452 86 12 346 Cuts at: 5942 1972 Size: 1977 Fragments arranged by size: 1977 1452 1305 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2056 2056 Size: 5947 BtgI C'CryG_G Cuts at: 513 1065 2122 2148 513 Size: 552 1057 26 4312 Fragments arranged by size: 4312 1057 552 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5208 Fragments arranged by size: 5208 683 56 BtsI GCAGTG_nn' Cuts at: 2229 2934 3463 4257 4277 2229 Size: 705 529 794 20 3899 Fragments arranged by size: 3899 794 705 529 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5947 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5912 DraI TTT'AAA Cuts at: 2479 3199 3251 2479 Size: 720 52 5175 Fragments arranged by size: 5175 720 52 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4954 809 Size: 507 3638 1802 Fragments arranged by size: 3638 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2099 3394 4283 Size: 54 1425 50 275 18 269 1295 889 Cuts at: 4283 5912 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1295 889 275 269 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2099 3394 1537 Size: 275 287 1295 4090 Fragments arranged by size: 4090 1295 287 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2652 3102 3877 1353 Size: 1299 450 775 3423 Fragments arranged by size: 3423 1299 775 450 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4534 4908 5054 387 Size: 1064 138 2945 374 146 1280 Fragments arranged by size: 2945 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2662 2662 Size: 5947 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2943 2961 4010 4607 5394 5662 1239 Size: 393 1311 18 1049 597 787 268 1524 Fragments arranged by size: 1524 1311 1049 787 597 393 268 18 EcoNI CCTnn'n_nnAGG Cuts at: 2793 2793 Size: 5947 EcoRI G'AATT_C Cuts at: 3256 3634 3256 Size: 378 5569 FspI TGC'GCA Cuts at: 2156 4453 2156 Size: 2297 3650 FspAI rTGC'GCAy Cuts at: 2156 2156 Size: 5947 HaeII r_GCGC'y Cuts at: 1678 2052 5094 1678 Size: 374 3042 2531 Fragments arranged by size: 3042 2531 374 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 2858 2890 3225 Size: 32 135 32 809 32 445 32 335 Cuts at: 3225 3257 4588 4620 4662 4694 1373 Size: 32 1331 32 42 32 2626 Fragments arranged by size: 2626 1331 809 445 335 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2133 2586 2760 2967 3291 3537 Size: 988 281 195 453 174 207 324 246 Cuts at: 3537 669 Size: 3079 Fragments arranged by size: 3079 988 453 324 281 246 207 195 174 HindIII A'AGCT_T Cuts at: 748 748 Size: 5947 HpaI GTT'AAC Cuts at: 2760 3537 2760 Size: 777 5170 KasI G'GCGC_C Cuts at: 2048 2048 Size: 5947 KpnI G_GTAC'C Cuts at: 2682 3272 2682 Size: 590 5357 MfeI C'AATT_G Cuts at: 3546 3546 Size: 5947 MluI A'CGCG_T Cuts at: 5823 5823 Size: 5947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2288 5061 5245 1305 Size: 983 2773 184 2007 Fragments arranged by size: 2773 2007 983 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4125 Fragments arranged by size: 4125 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2541 2753 3905 4264 4423 518 Size: 648 1375 212 1152 359 159 2042 Fragments arranged by size: 2042 1375 1152 648 359 212 159 MspA1I CmG'CkG Cuts at: 1606 2031 2124 3363 4040 5188 5433 1606 Size: 425 93 1239 677 1148 245 2120 Fragments arranged by size: 2120 1239 1148 677 425 245 93 NaeI GCC'GGC Cuts at: 2099 2099 Size: 5947 NarI GG'CG_CC Cuts at: 2049 2049 Size: 5947 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5395 NdeI CA'TA_TG Cuts at: 387 2764 387 Size: 2377 3570 NgoMIV G'CCGG_C Cuts at: 2097 2097 Size: 5947 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5947 NotI GC'GGCC_GC Cuts at: 3394 3394 Size: 5947 NspI r_CATG'y Cuts at: 1132 1229 3305 4850 1132 Size: 97 2076 1545 2229 Fragments arranged by size: 2229 2076 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5947 PciI A'CATG_T Cuts at: 4846 4846 Size: 5947 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5947 PfoI T'CCnGG_A Cuts at: 2169 3229 2169 Size: 1060 4887 PmeI GTTT'AAAC Cuts at: 3251 3251 Size: 5947 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4016 4048 5529 5561 4016 Size: 32 1481 32 4402 Fragments arranged by size: 4402 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5947 PsiI TTA'TAA Cuts at: 3517 3517 Size: 5947 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3201 3233 3201 Size: 32 5915 PstI C_TGCA'G Cuts at: 830 3299 830 Size: 2469 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5947 SacI G_AGCT'C Cuts at: 2664 2664 Size: 5947 SacII CC_GC'GG Cuts at: 2125 2125 Size: 5947 SalI G'TCGA_C Cuts at: 1936 3289 1936 Size: 1353 4594 SapI GCTCTTCn'nnn_ Cuts at: 2652 2652 Size: 5947 SbfI CC_TGCA'GG Cuts at: 3299 3299 Size: 5947 ScaI AGT'ACT Cuts at: 4195 5907 4195 Size: 1712 4235 SfcI C'TryA_G Cuts at: 826 1045 2179 3295 4430 5111 5302 826 Size: 219 1134 1116 1135 681 191 1471 Fragments arranged by size: 1471 1135 1134 1116 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2073 2098 2073 Size: 25 5922 SfoI GGC'GCC Cuts at: 2050 2050 Size: 5947 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5947 SmaI CCC'GGG Cuts at: 1872 2193 3274 1872 Size: 321 1081 4545 Fragments arranged by size: 4545 1081 321 SmlI C'TyrA_G Cuts at: 820 1017 1943 4051 4952 5214 820 Size: 197 926 2108 901 262 1553 Fragments arranged by size: 2108 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3738 493 Size: 3245 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5947 SphI G_CATG'C Cuts at: 1132 3305 1132 Size: 2173 3774 SrfI GCCC'GGGC Cuts at: 2193 2193 Size: 5947 SspI AAT'ATT Cuts at: 5 52 2578 2643 3871 5 Size: 47 2526 65 1228 2081 Fragments arranged by size: 2526 2081 1228 65 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5947 StyI C'CwwG_G Cuts at: 513 1065 2360 3386 513 Size: 552 1295 1026 3074 Fragments arranged by size: 3074 1295 1026 552 SwaI ATTT'AAAT Cuts at: 3199 3199 Size: 5947 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2594 3974 3991 4144 4329 4774 325 Size: 1255 1014 1380 17 153 185 445 1498 Fragments arranged by size: 1498 1380 1255 1014 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2335 4193 5905 Size: 274 80 33 51 1443 358 1858 1712 Cuts at: 5905 96 Size: 138 Fragments arranged by size: 1858 1712 1443 358 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5907 5939 5907 Size: 32 5915 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5947 XbaI T'CTAG_A Cuts at: 3283 3283 Size: 5947 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5947 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5947 XmaI C'CCGG_G Cuts at: 1870 2191 3272 1870 Size: 321 1081 4545 Fragments arranged by size: 4545 1081 321 XmnI GAAnn'nnTTC Cuts at: 2319 2523 4076 2319 Size: 204 1553 4190 Fragments arranged by size: 4190 1553 204 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3755 276 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I EcoRV FalI FseI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SanDI SexAI