################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AocI Aor51HI ApaI AscI Asp718I AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BglI BlnI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI ClaI CpoI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NarI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AciI 4 GCGG CCGC(-3/-1) AflII 1324 CTTAAG C^TTAAG AhdI 2299 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 394 GTGCAC G^TGCAC AlwNI 660 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 394 GTGCAC G^TGCAC AseI 1674 ATTAAT AT^TAAT AsnI 1674 ATTAAT AT^TAAT AspEI 2299 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 620 CCCGGG C^[CT]CG[GA]G BalI 1067 TGGCCA TGG^CCA BciVI 651 GTATCC GTATCC(6/5) BfrI 1324 CTTAAG C^TTAAG BlpI 2410 GCTAAGC GC^T[ACGT]AGC Bpu1102I 2410 GCTAAGC GC^T[ACGT]AGC BsaWI 436 ACCGGA [AT]^CCGG[AT] BsoBI 620 CCCGGG C^[CT]CG[GA]G Bsp1407I 1550 TGTACA T^GTACA BspMI 324 GCAGGT ACCTGC(4/8) BsrFI 2441 GCCGGC [GA]^CCGG[CT] BsrGI 1550 TGTACA T^GTACA BssSI 358 CACGAG CACGAG(-5/-1) BstXI 560 CCAATAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2410 GCTAAGC GC^T[ACGT]AGC Cfr10I 2441 GCCGGC [GA]^CCGG[CT] DraI 1306 TTTAAA TTT^AAA DraII 28 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 1210 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 2526 CCGTGG C^C[GA][CT]GG EaeI 1067 TGGCCA [CT]^GGCC[GA] Eam1105I 2299 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 28 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 802 GAATTC G^AATTC HindIII 1798 AAGCTT A^AGCTT MaeII 1613 ACGT A^CGT MluNI 1067 TGGCCA TGG^CCA MscI 1067 TGGCCA TGG^CCA MspA1I 1142 CAGCTG C[AC]G^C[GT]G NaeI 2441 GCCGGC GCC^GGC NgoMIV 2441 GCCGGC G^CCGGC NheI 142 GCTAGC G^CTAGC PpuMI 28 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 705 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1674 ATTAAT AT^TAAT PvuII 1142 CAGCTG CAG^CTG SexAI 1211 ACCTGGT A^CC[AT]GGT SmaI 620 CCCGGG CCC^GGG SphI 738 GCATGC GCATG^C SspBI 1550 TGTACA T^GTACA SspI 1162 AATATT AAT^ATT StuI 901 AGGCCT AGG^CCT XmaCI 620 CCCGGG C^CCGGG XmaI 620 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Asp700I 121 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 349 GAAGAATTTC AvaII 29 GGTCC G^G[AT]CC 2530 GGACC BclI 834 TGATCA T^GATCA 1089 TGATCA BcnI 620 CCCGG CC^[GC]GG 621 CCGGG BfaI 143 CTAG C^TAG 905 CTAG BpmI 344 CTGGAG CTGGAG(16/14) 1451 CTGGAG BsaI 1079 GAGACC GGTCTC(1/5) 1811 GAGACC BsgI 2492 CTGCAC GTGCAG(16/14) 322 GTGCAG BsiYI 1815 CCACCATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2184 CCGTATTAAGG BslI 1815 CCACCATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 2184 CCGTATTAAGG FbaI 834 TGATCA T^GATCA 1089 TGATCA MaeI 143 CTAG C^TAG 905 CTAG NciI 620 CCCGG CC^[GC]GG 621 CCGGG PstI 657 CTGCAG CTGCA^G 2511 CTGCAG SmlI 1324 CTTAAG C^T[CT][GA]AG 1930 CTTGAG Tsp45I 433 GTGAC ^GT[GC]AC 682 GTGAC XcmI 899 CCAGGCCTAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1254 CCAGAAAATTCTTGG XmnI 121 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 349 GAAGAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AcsI AfaI AflIII AluI AlwI ApoI AspHI BbsI BbvI BglII BmyI BpuAI BsaJI BseRI BsiHKAI BsmAI BsmFI Bsp1286I BspLU11I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII