(Circular) (Six-base) MAPSORT of: pFN21AE4234.seq Check: 4867 from: 1 to: 7267 pFN21AE4234 7267 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5077 278 Size: 53 83 186 936 3541 2468 Fragments arranged by size: 3541 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4610 1937 Size: 2673 4594 Acc65I G'GTAC_C Cuts at: 4588 4588 Size: 7267 AclI AA'CG_TT Cuts at: 1563 5394 5767 1563 Size: 3831 373 3063 Fragments arranged by size: 3831 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 2117 3045 4034 4340 4408 5330 6714 Size: 482 3 928 989 306 68 922 1384 Cuts at: 6714 6982 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 989 928 922 482 306 268 68 3 AflII C'TTAA_G Cuts at: 820 1017 3329 820 Size: 197 2312 4758 Fragments arranged by size: 4758 2312 197 AflIII A'CryG_T Cuts at: 1203 2652 4162 4452 6166 7143 1203 Size: 1449 1510 290 1714 977 1327 Fragments arranged by size: 1714 1510 1449 1327 977 290 AgeI A'CCGG_T Cuts at: 6098 6098 Size: 7267 AhdI GACnn_n'nnGTC Cuts at: 4309 5996 4309 Size: 1687 5580 AleI CACnn'nnGTG Cuts at: 1166 2301 2618 1166 Size: 1135 317 5815 Fragments arranged by size: 5815 1135 317 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2167 3271 4522 1184 Size: 983 1104 1251 3929 Fragments arranged by size: 3929 1251 1104 983 AlwNI CAG_nnn'CTG Cuts at: 2670 5010 6582 2670 Size: 2340 1572 3355 Fragments arranged by size: 3355 2340 1572 ApaLI G'TGCA_C Cuts at: 2399 5324 6480 2399 Size: 2925 1156 3186 Fragments arranged by size: 3186 2925 1156 ApoI r'AATT_y Cuts at: 1493 2357 2807 3264 3308 3404 3735 4058 4239 Size: 864 450 457 44 96 331 323 181 Cuts at: 4239 4263 4576 4802 4954 7063 1493 Size: 24 313 226 152 2109 1697 Fragments arranged by size: 2109 1697 864 457 450 331 323 313 226 181 152 96 44 24 AseI AT'TA_AT Cuts at: 160 1032 3680 5821 160 Size: 872 2648 2141 1606 Fragments arranged by size: 2648 2141 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7267 AvaI C'yCGr_G Cuts at: 1870 1943 2625 4592 1870 Size: 73 682 1967 4545 Fragments arranged by size: 4545 1967 682 73 BamHI G'GATC_C Cuts at: 4597 4964 4597 Size: 367 6900 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4588 6043 618 Size: 325 217 627 2801 1455 1842 Fragments arranged by size: 2801 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4586 1391 Size: 3195 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2633 3774 4429 928 Size: 971 734 1141 655 3766 Fragments arranged by size: 3766 1141 971 734 655 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5458 5492 1903 Size: 34 3521 34 3678 Fragments arranged by size: 3678 3521 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2667 5159 6369 2667 Size: 2492 1210 3565 Fragments arranged by size: 3565 2492 1210 BclI T'GATC_A Cuts at: 1548 2839 3094 1548 Size: 1291 255 5721 Fragments arranged by size: 5721 1291 255 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5878 136 Size: 107 122 71 631 605 4206 1525 Fragments arranged by size: 4206 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2952 3460 3939 7262 2952 Size: 508 479 3323 2957 Fragments arranged by size: 3323 2957 508 479 BlpI GC'TnA_GC Cuts at: 4416 4684 4416 Size: 268 6999 Bme1580I G_kGCm'C Cuts at: 1790 2189 2403 2625 4012 5328 6484 1790 Size: 399 214 222 1387 1316 1156 2573 Fragments arranged by size: 2573 1387 1316 1156 399 222 214 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7267 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2040 4998 5956 Size: 416 53 83 399 24 623 2958 958 Cuts at: 5956 442 Size: 1753 Fragments arranged by size: 2958 1753 958 623 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2151 1056 Size: 1095 6172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2370 3477 5927 1239 Size: 1131 1107 2450 2579 Fragments arranged by size: 2579 2450 1131 1107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3956 5392 6257 6555 3956 Size: 1436 865 298 4668 Fragments arranged by size: 4668 1436 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3078 3810 5930 882 Size: 617 1579 732 2120 2219 Fragments arranged by size: 2219 2120 1579 732 617 BsaAI yAC'GTr Cuts at: 493 1204 5058 493 Size: 711 3854 2702 Fragments arranged by size: 3854 2702 711 BsaBI GATnn'nnATC Cuts at: 7261 7261 Size: 7267 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5074 5456 6091 275 Size: 53 83 186 936 3541 382 635 1451 Fragments arranged by size: 3541 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2441 5700 6098 6372 6519 6930 2441 Size: 3259 398 274 147 411 2778 Fragments arranged by size: 3259 2778 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7042 Fragments arranged by size: 7042 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 3243 4379 1186 Size: 847 1210 1136 4074 Fragments arranged by size: 4074 1210 1136 847 BseYI C'CCAG_C Cuts at: 2703 4481 6470 6966 2703 Size: 1778 1989 496 3004 Fragments arranged by size: 3004 1989 1778 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2348 4482 1163 Size: 573 612 2134 3948 Fragments arranged by size: 3948 2134 612 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4717 5478 6506 1540 Size: 275 186 2716 761 1028 2301 Fragments arranged by size: 2716 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2298 2403 2729 5328 5413 6484 2298 Size: 105 326 2599 85 1071 3081 Fragments arranged by size: 3081 2599 1071 326 105 85 BsmI GAATG_Cn' Cuts at: 4778 4871 4778 Size: 93 7174 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2189 2298 2403 2625 2729 4012 4586 Size: 399 399 109 105 222 104 1283 574 Cuts at: 4586 5328 5413 6484 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1283 1071 742 574 399 399 222 109 105 104 85 BspEI T'CCGG_A Cuts at: 6930 6930 Size: 7267 BspHI T'CATG_A Cuts at: 5156 6886 5156 Size: 1730 5537 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2320 4622 844 Size: 1476 2302 3489 Fragments arranged by size: 3489 2302 1476 BsrBI CCG'CTC Cuts at: 1394 1823 5154 1394 Size: 429 3331 3507 Fragments arranged by size: 3507 3331 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3586 3913 4441 5762 5936 65 Size: 1183 2338 327 528 1321 174 1396 Fragments arranged by size: 2338 1396 1321 1183 528 327 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4446 5911 6098 1700 Size: 110 2636 1465 187 2869 Fragments arranged by size: 2869 2636 1465 187 110 BsrGI T'GTAC_A Cuts at: 96 3555 96 Size: 3459 3808 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7267 BssSI C'ACGA_G Cuts at: 2363 5327 6339 2363 Size: 2964 1012 3291 Fragments arranged by size: 3291 2964 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2670 4267 4515 794 Size: 1876 1597 248 3546 Fragments arranged by size: 3546 1876 1597 248 BstBI TT'CG_AA Cuts at: 6071 6071 Size: 7267 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7267 BstXI CCAn_nnnn'nTGG Cuts at: 2572 2572 Size: 7267 BstYI r'GATC_y Cuts at: 1972 2417 2952 3460 3939 4209 4597 4964 5349 Size: 445 535 508 479 270 388 367 385 Cuts at: 5349 5366 6818 6904 6916 7262 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 535 508 479 445 388 385 367 346 270 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 4531 513 Size: 552 939 2527 3249 Fragments arranged by size: 3249 2527 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6528 Fragments arranged by size: 6528 683 56 BtsI GCAGTG_nn' Cuts at: 4525 4783 5577 5597 4525 Size: 258 794 20 6195 Fragments arranged by size: 6195 794 258 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7267 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7232 DraI TTT'AAA Cuts at: 3313 4571 3313 Size: 1258 6009 DraIII CAC_nnn'GTG Cuts at: 3220 3220 Size: 7267 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6274 809 Size: 507 4958 1802 Fragments arranged by size: 4958 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3072 4714 5603 Size: 54 1425 50 275 18 1242 1642 889 Cuts at: 5603 7232 8 Size: 1629 43 Fragments arranged by size: 1642 1629 1425 1242 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4714 1537 Size: 275 2902 4090 Fragments arranged by size: 4090 2902 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 2073 2943 2962 3960 5197 1353 Size: 711 9 870 19 998 1237 3423 Fragments arranged by size: 3423 1237 998 870 711 19 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5854 6228 6374 387 Size: 1064 138 435 3830 374 146 1280 Fragments arranged by size: 3830 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2117 2370 3045 3477 4034 4340 Size: 393 482 3 253 675 432 557 306 Cuts at: 4340 4408 5330 5927 6714 6982 1239 Size: 68 922 597 787 268 1524 Fragments arranged by size: 1524 922 787 675 597 557 482 432 393 306 268 253 68 3 EcoO109I rG'GnC_Cy Cuts at: 2034 2034 Size: 7267 EcoRI G'AATT_C Cuts at: 2807 4576 4954 2807 Size: 1769 378 5120 Fragments arranged by size: 5120 1769 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4370 4370 Size: 7267 FspI TGC'GCA Cuts at: 5773 5773 Size: 7267 HaeII r_GCGC'y Cuts at: 1678 6414 1678 Size: 4736 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2533 2565 2582 2614 2904 Size: 32 135 32 961 32 17 32 290 Cuts at: 2904 2936 3961 3993 4295 4327 5908 5940 5982 Size: 32 1025 32 302 32 1581 32 42 Cuts at: 5982 6014 1373 Size: 32 2626 Fragments arranged by size: 2626 1581 1025 961 302 290 135 42 32 32 32 32 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 669 1657 1938 3709 3947 4168 4611 4857 669 Size: 988 281 1771 238 221 443 246 3079 Fragments arranged by size: 3079 1771 988 443 281 246 238 221 HindIII A'AGCT_T Cuts at: 748 3803 748 Size: 3055 4212 HpaI GTT'AAC Cuts at: 4857 4857 Size: 7267 KpnI G_GTAC'C Cuts at: 4592 4592 Size: 7267 MfeI C'AATT_G Cuts at: 4866 4866 Size: 7267 MluI A'CGCG_T Cuts at: 7143 7143 Size: 7267 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6381 6565 1305 Size: 5076 184 2007 Fragments arranged by size: 5076 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3074 10 Size: 54 1425 343 1242 4203 Fragments arranged by size: 4203 1425 1242 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2301 2303 2618 2748 3253 5225 5584 Size: 648 1135 2 315 130 505 1972 359 Cuts at: 5584 5743 518 Size: 159 2042 Fragments arranged by size: 2042 1972 1135 648 505 359 315 159 130 2 MspA1I CmG'CkG Cuts at: 1606 3149 4683 5360 6508 6753 1606 Size: 1543 1534 677 1148 245 2120 Fragments arranged by size: 2120 1543 1534 1148 677 245 NaeI GCC'GGC Cuts at: 4448 4448 Size: 7267 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5776 Fragments arranged by size: 5776 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7267 NgoMIV G'CCGG_C Cuts at: 4446 4446 Size: 7267 NheI G'CTAG_C Cuts at: 1052 2147 1052 Size: 1095 6172 NotI GC'GGCC_GC Cuts at: 4714 4714 Size: 7267 NspI r_CATG'y Cuts at: 1132 1229 2656 2747 3715 3791 4166 4456 4625 Size: 97 1427 91 968 76 375 290 169 Cuts at: 4625 6170 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1427 968 375 290 169 97 91 76 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7267 PciI A'CATG_T Cuts at: 2652 4162 4452 6166 2652 Size: 1510 290 1714 3753 Fragments arranged by size: 3753 1714 1510 290 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7267 PmeI GTTT'AAAC Cuts at: 4571 4571 Size: 7267 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5336 5368 6849 6881 5336 Size: 32 1481 32 5722 Fragments arranged by size: 5722 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2034 2034 Size: 7267 PshAI GACnn'nnGTC Cuts at: 1613 2714 1613 Size: 1101 6166 PsiI TTA'TAA Cuts at: 3306 4837 3306 Size: 1531 5736 PstI C_TGCA'G Cuts at: 830 2666 4520 4619 830 Size: 1836 1854 99 3478 Fragments arranged by size: 3478 1854 1836 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7267 PvuII CAG'CTG Cuts at: 3149 3149 Size: 7267 SalI G'TCGA_C Cuts at: 1936 4609 1936 Size: 2673 4594 SanDI GG'GwC_CC Cuts at: 2034 2034 Size: 7267 SbfI CC_TGCA'GG Cuts at: 4619 4619 Size: 7267 ScaI AGT'ACT Cuts at: 5515 7227 5515 Size: 1712 5555 SexAI A'CCwGG_T Cuts at: 3216 3216 Size: 7267 SfcI C'TryA_G Cuts at: 826 1045 2049 2662 3782 4516 4615 5750 6431 Size: 219 1004 613 1120 734 99 1135 681 Cuts at: 6431 6622 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1120 1004 734 681 613 219 191 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7267 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7267 SmaI CCC'GGG Cuts at: 1872 2627 4594 1872 Size: 755 1967 4545 Fragments arranged by size: 4545 1967 755 SmlI C'TyrA_G Cuts at: 820 1017 1943 3329 3935 5371 6272 6534 820 Size: 197 926 1386 606 1436 901 262 1553 Fragments arranged by size: 1553 1436 1386 926 901 606 262 197 SnaBI TAC'GTA Cuts at: 493 5058 493 Size: 4565 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7267 SphI G_CATG'C Cuts at: 1132 2747 4625 1132 Size: 1615 1878 3774 Fragments arranged by size: 3774 1878 1615 SspI AAT'ATT Cuts at: 5 52 3169 5191 5 Size: 47 3117 2022 2081 Fragments arranged by size: 3117 2081 2022 47 StuI AGG'CCT Cuts at: 1517 2908 1517 Size: 1391 5876 StyI C'CwwG_G Cuts at: 513 1065 2004 3075 4065 4230 4706 513 Size: 552 939 1071 990 165 476 3074 Fragments arranged by size: 3074 1071 990 939 552 476 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2291 2418 2564 5294 5311 5464 5649 Size: 1255 711 127 146 2730 17 153 185 Cuts at: 5649 6094 325 Size: 445 1498 Fragments arranged by size: 2730 1498 1255 711 445 185 153 146 127 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2851 3555 5513 Size: 274 80 33 51 1443 874 704 1958 Cuts at: 5513 7225 96 Size: 1712 138 Fragments arranged by size: 1958 1712 1443 874 704 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7227 7259 7227 Size: 32 7235 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7267 XbaI T'CTAG_A Cuts at: 4603 4603 Size: 7267 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2911 3266 2911 Size: 355 6912 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7267 XmaI C'CCGG_G Cuts at: 1870 2625 4592 1870 Size: 755 1967 4545 Fragments arranged by size: 4545 1967 755 XmnI GAAnn'nnTTC Cuts at: 2130 2358 5396 2130 Size: 228 3038 4001 Fragments arranged by size: 4001 3038 228 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5075 276 Size: 53 83 186 936 3541 2468 Fragments arranged by size: 3541 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI