(Linear) (Six-base) MAPSORT of: KIEE4234.seq Check: 7265 from: 1 to: 2562 KIEE4234.orf With 182 enzymes: SgfI * July 3, 2012 18:50 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 112 1040 2029 2335 2403 2562 Size: 109 3 928 989 306 68 159 Fragments arranged by size: 989 928 306 159 109 68 3 AflII C'TTAA_G Cuts at: 0 1324 2562 Size: 1324 1238 AflIII A'CryG_T Cuts at: 0 647 2157 2447 2562 Size: 647 1510 290 115 Fragments arranged by size: 1510 647 290 115 AhdI GACnn_n'nnGTC Cuts at: 0 2304 2562 Size: 2304 258 AleI CACnn'nnGTG Cuts at: 0 296 613 2562 Size: 296 317 1949 Fragments arranged by size: 1949 317 296 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 162 1266 2517 2562 Size: 162 1104 1251 45 Fragments arranged by size: 1251 1104 162 45 AlwNI CAG_nnn'CTG Cuts at: 0 665 2562 Size: 665 1897 ApaLI G'TGCA_C Cuts at: 0 394 2562 Size: 394 2168 ApoI r'AATT_y Cuts at: 0 352 802 1259 1303 1399 1730 2053 2234 Size: 352 450 457 44 96 331 323 181 Cuts at: 2234 2258 2562 Size: 24 304 Fragments arranged by size: 457 450 352 331 323 304 181 96 44 24 AseI AT'TA_AT Cuts at: 0 1675 2562 Size: 1675 887 AvaI C'yCGr_G Cuts at: 0 620 2562 Size: 620 1942 BbsI GAAGACnn'nnnn_ Cuts at: 0 628 1769 2424 2562 Size: 628 1141 655 138 Fragments arranged by size: 1141 655 628 138 BciVI GTATCCnnnnn_n' Cuts at: 0 662 2562 Size: 662 1900 BclI T'GATC_A Cuts at: 0 834 1089 2562 Size: 834 255 1473 Fragments arranged by size: 1473 834 255 BglII A'GATC_T Cuts at: 0 947 1455 1934 2562 Size: 947 508 479 628 Fragments arranged by size: 947 628 508 479 BlpI GC'TnA_GC Cuts at: 0 2411 2562 Size: 2411 151 Bme1580I G_kGCm'C Cuts at: 0 184 398 620 2007 2562 Size: 184 214 222 1387 555 Fragments arranged by size: 1387 555 222 214 184 BmrI ACTGGGnnnn_n' Cuts at: 0 35 2562 Size: 35 2527 BmtI G_CTAG'C Cuts at: 0 146 2562 Size: 146 2416 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 365 1472 2562 Size: 365 1107 1090 Fragments arranged by size: 1107 1090 365 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1951 2562 Size: 1951 611 BsaI GGTCTCn'nnnn_ Cuts at: 0 1073 1805 2562 Size: 1073 732 757 Fragments arranged by size: 1073 757 732 BsaWI w'CCGG_w Cuts at: 0 436 2562 Size: 436 2126 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 28 1238 2374 2562 Size: 28 1210 1136 188 Fragments arranged by size: 1210 1136 188 28 BseYI C'CCAG_C Cuts at: 0 698 2476 2562 Size: 698 1778 86 Fragments arranged by size: 1778 698 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 2477 2562 Size: 343 2134 85 Fragments arranged by size: 2134 343 85 BsiHKAI G_wGCw'C Cuts at: 0 293 398 724 2562 Size: 293 105 326 1838 Fragments arranged by size: 1838 326 293 105 Bsp1286I G_dGCh'C Cuts at: 0 184 293 398 620 724 2007 2562 Size: 184 109 105 222 104 1283 555 Fragments arranged by size: 1283 555 222 184 109 105 104 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 315 2562 Size: 315 2247 BsrDI GCAATG_nn' Cuts at: 0 1581 1908 2436 2562 Size: 1581 327 528 126 Fragments arranged by size: 1581 528 327 126 BsrFI r'CCGG_y Cuts at: 0 2441 2562 Size: 2441 121 BsrGI T'GTAC_A Cuts at: 0 1550 2562 Size: 1550 1012 BssSI C'ACGA_G Cuts at: 0 358 2562 Size: 358 2204 BstAPI GCAn_nnn'nTGC Cuts at: 0 665 2262 2510 2562 Size: 665 1597 248 52 Fragments arranged by size: 1597 665 248 52 BstXI CCAn_nnnn'nTGG Cuts at: 0 567 2562 Size: 567 1995 BstYI r'GATC_y Cuts at: 0 412 947 1455 1934 2204 2562 Size: 412 535 508 479 270 358 Fragments arranged by size: 535 508 479 412 358 270 BtgI C'CryG_G Cuts at: 0 2526 2562 Size: 2526 36 BtsI GCAGTG_nn' Cuts at: 0 2520 2562 Size: 2520 42 DraI TTT'AAA Cuts at: 0 1308 2562 Size: 1308 1254 DraIII CAC_nnn'GTG Cuts at: 0 1215 2562 Size: 1215 1347 EaeI y'GGCC_r Cuts at: 0 1067 2562 Size: 1067 1495 EarI CTCTTCn'nnn_ Cuts at: 0 59 68 938 957 1955 2562 Size: 59 9 870 19 998 607 Fragments arranged by size: 998 870 607 59 19 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 2562 Size: 19 2543 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 112 365 1040 1472 2029 2335 2403 Size: 109 3 253 675 432 557 306 68 Cuts at: 2403 2562 Size: 159 Fragments arranged by size: 675 557 432 306 253 159 109 68 3 EcoO109I rG'GnC_Cy Cuts at: 0 29 2562 Size: 29 2533 EcoRI G'AATT_C Cuts at: 0 802 2562 Size: 802 1760 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2365 2562 Size: 2365 197 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 528 560 577 609 899 931 1956 1988 Size: 528 32 17 32 290 32 1025 32 Cuts at: 1988 2290 2322 2562 Size: 302 32 240 Fragments arranged by size: 1025 528 302 290 240 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 0 1704 1942 2163 2562 Size: 1704 238 221 399 Fragments arranged by size: 1704 399 238 221 HindIII A'AGCT_T Cuts at: 0 1798 2562 Size: 1798 764 MscI TGG'CCA Cuts at: 0 1069 2562 Size: 1069 1493 MslI CAynn'nnrTG Cuts at: 0 296 298 613 743 1248 2562 Size: 296 2 315 130 505 1314 Fragments arranged by size: 1314 505 315 296 130 2 MspA1I CmG'CkG Cuts at: 0 1144 2562 Size: 1144 1418 NaeI GCC'GGC Cuts at: 0 2443 2562 Size: 2443 119 NgoMIV G'CCGG_C Cuts at: 0 2441 2562 Size: 2441 121 NheI G'CTAG_C Cuts at: 0 142 2562 Size: 142 2420 NspI r_CATG'y Cuts at: 0 651 742 1710 1786 2161 2451 2562 Size: 651 91 968 76 375 290 111 Fragments arranged by size: 968 651 375 290 111 91 76 PciI A'CATG_T Cuts at: 0 647 2157 2447 2562 Size: 647 1510 290 115 Fragments arranged by size: 1510 647 290 115 PpuMI rG'GwC_Cy Cuts at: 0 29 2562 Size: 29 2533 PshAI GACnn'nnGTC Cuts at: 0 709 2562 Size: 709 1853 PsiI TTA'TAA Cuts at: 0 1301 2562 Size: 1301 1261 PstI C_TGCA'G Cuts at: 0 661 2515 2562 Size: 661 1854 47 Fragments arranged by size: 1854 661 47 PvuII CAG'CTG Cuts at: 0 1144 2562 Size: 1144 1418 SanDI GG'GwC_CC Cuts at: 0 29 2562 Size: 29 2533 SexAI A'CCwGG_T Cuts at: 0 1211 2562 Size: 1211 1351 SfcI C'TryA_G Cuts at: 0 44 657 1777 2511 2562 Size: 44 613 1120 734 51 Fragments arranged by size: 1120 734 613 51 44 SmaI CCC'GGG Cuts at: 0 622 2562 Size: 622 1940 SmlI C'TyrA_G Cuts at: 0 1324 1930 2562 Size: 1324 606 632 Fragments arranged by size: 1324 632 606 SphI G_CATG'C Cuts at: 0 742 2562 Size: 742 1820 SspI AAT'ATT Cuts at: 0 1164 2562 Size: 1164 1398 StuI AGG'CCT Cuts at: 0 903 2562 Size: 903 1659 StyI C'CwwG_G Cuts at: 0 1070 2060 2225 2562 Size: 1070 990 165 337 Fragments arranged by size: 1070 990 337 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 286 413 559 2562 Size: 286 127 146 2003 Fragments arranged by size: 2003 286 146 127 TatI w'GTAC_w Cuts at: 0 846 1550 2562 Size: 846 704 1012 Fragments arranged by size: 1012 846 704 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 906 1261 2562 Size: 906 355 1301 Fragments arranged by size: 1301 906 355 XmaI C'CCGG_G Cuts at: 0 620 2562 Size: 620 1942 XmnI GAAnn'nnTTC Cuts at: 0 125 353 2562 Size: 125 228 2209 Fragments arranged by size: 2209 228 125 Enzymes that do cut: AcuI AflII AflIII AhdI AleI AlfI AlwNI ApaLI ApoI AseI AvaI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BmtI BpmI BpuEI BsaI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtsI DraI DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII MscI MslI MspA1I NaeI NgoMIV NheI NspI PciI PpuMI PshAI PsiI PstI PvuII SanDI SexAI SfcI SmaI SmlI SphI SspI StuI StyI TaqII TatI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AgeI AloI ApaI AscI AsiSI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BfrBI BglI BmgBI BplI Bpu10I BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EagI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NdeI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI TstI Tth111I XbaI XhoI ZraI