################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MflI MluI MroI MslI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 451 GTCTAC GT^[AC][GT]AC AciI 372 CCGC CCGC(-3/-1) AlwI 699 GATCC GGATC(4/5) ApaI 593 GGGCCC GGGCC^C AseI 316 ATTAAT AT^TAAT AsnI 316 ATTAAT AT^TAAT AspI 564 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 345 GGCACC G^G[CT][GA]CC BbsI 994 GTCTTC GAAGAC(2/6) BbvCI 423 CCTCAGC CCTCAGC(-5/-2) BcnI 44 CCGGG CC^[GC]GG BpuAI 994 GTCTTC GAAGAC(2/6) BseRI 225 GAGGAG GAGGAG(10/8) BsmBI 1033 CGTCTC CGTCTC(1/5) BstAPI 381 GCAGGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 1057 GCGC GCG^C EcoT14I 1126 CCAAGG C^C[AT][AT]GG HapII 44 CCGG C^CGG HgaI 51 GACGC GACGC(5/10) HhaI 1057 GCGC GCG^C HinP1I 1057 GCGC G^CGC HincII 298 GTCAAC GT[CT]^[GA]AC HindII 298 GTCAAC GT[CT]^[GA]AC HpaII 44 CCGG C^CGG HphI 577 GGTGA GGTGA(8/7) MaeII 410 ACGT A^CGT MfeI 154 CAATTG C^AATTG MspA1I 265 CAGCTG C[AC]G^C[GT]G MspI 44 CCGG C^CGG MunI 154 CAATTG C^AATTG MwoI 381 GCAGGTTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 44 CCGGG CC^[GC]GG PflFI 564 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 316 ATTAAT AT^TAAT PstI 1020 CTGCAG CTGCA^G PvuII 265 CAGCTG CAG^CTG SapI 1120 GCTCTTC GCTCTTC(1/4) SbfI 1019 CCTGCAGG CCTGCA^GG SexAI 353 ACCTGGT A^CC[AT]GGT SfcI 1020 CTGCAG C^T[GA][CT]AG Sse8387I 1019 CCTGCAGG CCTGCA^GG StyI 1126 CCAAGG C^C[AT][AT]GG TaqI 29 TCGA T^CGA Tth111I 564 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 29 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwNI 49 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG 946 CAGAACCTG BalI 269 TGGCCA TGG^CCA 1099 TGGCCA BanII 593 GGGCCC G[GA]GC[CT]^C 1118 GGGCTC BclI 545 TGATCA T^GATCA 938 TGATCA BmyI 593 GGGCCC G[AGT]GC[ACT]^C 1118 GGGCTC BsgI 583 GTGCAG GTGCAG(16/14) 768 GTGCAG BsiYI 218 CCACATGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1017 CCCCTGCAGGG BslI 218 CCACATGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1017 CCCCTGCAGGG BsmAI 335 GAGAC GTCTC(1/5) 1034 GTCTC BsmFI 40 GGGAC GGGAC(10/14) 420 GTCCC Bsp1286I 593 GGGCCC G[AGT]GC[ACT]^C 1118 GGGCTC BspMI 950 ACCTGC ACCTGC(4/8) 381 GCAGGT BstF5I 660 CATCC GGATG(2/0) 1029 CATCC EaeI 269 TGGCCA [CT]^GGCC[GA] 1099 TGGCCA EarI 1121 CTCTTC CTCTTC(1/4) 558 GAAGAG FbaI 545 TGATCA T^GATCA 938 TGATCA FokI 660 CATCC GGATG(9/13) 1029 CATCC Ksp632I 1121 CTCTTC CTCTTC(1/4) 558 GAAGAG MaeIII 88 GTGAC ^GT[ACGT]AC 259 GTGAC MluNI 269 TGGCCA TGG^CCA 1099 TGGCCA MscI 269 TGGCCA TGG^CCA 1099 TGGCCA PpuMI 170 GGGTCCT [GA]G^G[AT]CC[CT] 491 GGGTCCT SfaNI 680 GCATC GCATC(5/9) 827 GCATC Tsp45I 88 GTGAC ^GT[GC]AC 259 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AfaI AluI AvaII BbvI BpmI BsaJI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HinfI ItaI MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp509I TspRI