################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MfeI MflI MluI MroI MslI MunI NaeI NarI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 529 CGCG CG^CG AfaI 30 GTAC GT^AC ApaI 784 GGGCCC GGGCC^C Asp700I 36 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 97 CCCGAG C^[CT]CG[GA]G BanII 784 GGGCCC G[GA]GC[CT]^C BglI 149 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsmAI 131 GTCTC GTCTC(1/5) BsoBI 97 CCCGAG C^[CT]CG[GA]G BstAPI 39 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 579 GGTGACC G^GT[ACGT]ACC BstF5I 678 GGATG GGATG(2/0) BstPI 579 GGTGACC G^GT[ACGT]ACC BstUI 529 CGCG CG^CG CfoI 530 GCGC GCG^C DdeI 189 CTCAG C^T[ACGT]AG DraIII 574 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 494 CCATGG C^C[GA][CT]GG EagI 363 CGGCCG C^GGCCG EclXI 363 CGGCCG C^GGCCG Eco52I 363 CGGCCG C^GGCCG EcoO65I 579 GGTGACC G^GT[ACGT]ACC FokI 678 GGATG GGATG(9/13) HhaI 530 GCGC GCG^C HinP1I 530 GCGC G^CGC HinfI 226 GATTC G^A[ACGT]TC MboII 294 GAAGA GAAGA(8/7) MseI 119 TTAA T^TAA MvnI 529 CGCG CG^CG NcoI 494 CCATGG C^CATGG PpuMI 192 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 582 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 30 GTAC GT^AC SexAI 575 ACCTGGT A^CC[AT]GGT SfiI 148 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 156 AGGCCT AGG^CCT TaqI 80 TCGA T^CGA TfiI 226 GATTC G^A[AT]TC Tru9I 119 TTAA T^TAA TthHB8I 80 TCGA T^CGA XcmI 50 CCAGGGACTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 36 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 334 AAATTC [GA]^AATT[CT] 628 GAATTT ApoI 334 AAATTC [GA]^AATT[CT] 628 GAATTT AspHI 412 GTGCTC G[AT]GC[AT]^C 478 GTGCTC AvaII 193 GGTCC G^G[AT]CC 320 GGACC BalI 491 TGGCCA TGG^CCA 570 TGGCCA BpmI 152 CTGGAG CTGGAG(16/14) 646 CTGGAG BsiEI 261 CGACCG CG[GA][CT]^CG 363 CGGCCG BsiHKAI 412 GTGCTC G[AT]GC[AT]^C 478 GTGCTC BsrI 16 ACTGG ACTGG(1/-1) 317 ACTGG DraII 192 AGGTCCT [GA]G^G[ACGT]CC[CT] 307 GGGGCCT EcoO109I 192 AGGTCCT [GA]G^G[ACGT]CC[CT] 307 GGGGCCT EcoT14I 162 CCAAGG C^C[AT][AT]GG 494 CCATGG MluNI 491 TGGCCA TGG^CCA 570 TGGCCA MscI 491 TGGCCA TGG^CCA 570 TGGCCA NlaIII 495 CATG CATG^ 612 CATG SfcI 72 CTACAG C^T[GA][CT]AG 510 CTGTAG SmlI 415 CTCAAG C^T[CT][GA]AG 559 CTCAAG StyI 162 CCAAGG C^C[AT][AT]GG 494 CCATGG Tsp509I 335 AATT ^AATT 629 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AluI BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I Cfr13I EaeI EcoRII FauI Fnu4HI HaeIII HapII HpaII HphI ItaI MaeIII MnlI MspA1I MspI MvaI MwoI NciI NlaIV Sau96I ScrFI SfaNI TseI Tsp45I TspRI