(Circular) (Six-base) MAPSORT of: pFN21AE1759.seq Check: 7288 from: 1 to: 5494 pFN21AE1759 5494 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3304 278 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2837 1937 Size: 900 4594 Acc65I G'GTAC_C Cuts at: 2815 2815 Size: 5494 AclI AA'CG_TT Cuts at: 1563 3621 3994 1563 Size: 2058 373 3063 Fragments arranged by size: 3063 2058 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2138 2318 3557 4941 5209 1632 Size: 506 180 1239 1384 268 1917 Fragments arranged by size: 1917 1384 1239 506 268 180 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5297 AflIII A'CryG_T Cuts at: 1203 4393 5370 1203 Size: 3190 977 1327 Fragments arranged by size: 3190 1327 977 AgeI A'CCGG_T Cuts at: 4325 4325 Size: 5494 AhdI GACnn_n'nnGTC Cuts at: 4223 4223 Size: 5494 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5494 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5494 AlwNI CAG_nnn'CTG Cuts at: 3237 4809 3237 Size: 1572 3922 ApaI G_GGCC'C Cuts at: 2793 2793 Size: 5494 ApaLI G'TGCA_C Cuts at: 3551 4707 3551 Size: 1156 4338 ApoI r'AATT_y Cuts at: 1493 2339 2633 2803 3029 3181 5290 1493 Size: 846 294 170 226 152 2109 1697 Fragments arranged by size: 2109 1697 846 294 226 170 152 AseI AT'TA_AT Cuts at: 160 1032 4048 160 Size: 872 3016 1606 Fragments arranged by size: 3016 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 AvaI C'yCGr_G Cuts at: 1870 1943 2102 2819 1870 Size: 73 159 717 4545 Fragments arranged by size: 4545 717 159 73 BamHI G'GATC_C Cuts at: 2824 3191 2824 Size: 367 5127 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2815 4270 618 Size: 325 217 627 1028 1455 1842 Fragments arranged by size: 1842 1455 1028 627 325 217 BanII G_rGCy'C Cuts at: 1391 2793 2813 1391 Size: 1402 20 4072 Fragments arranged by size: 4072 1402 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4523 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3685 3719 1903 Size: 34 1748 34 3678 Fragments arranged by size: 3678 1748 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3386 4596 3386 Size: 1210 4284 BclI T'GATC_A Cuts at: 1548 1548 Size: 5494 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2160 4105 136 Size: 107 122 71 631 605 488 1945 1525 Fragments arranged by size: 1945 1525 631 605 488 122 107 71 BglII A'GATC_T Cuts at: 5489 5489 Size: 5494 BlpI GC'TnA_GC Cuts at: 2911 2911 Size: 5494 Bme1580I G_kGCm'C Cuts at: 1790 2793 3555 4711 1790 Size: 1003 762 1156 2573 Fragments arranged by size: 2573 1156 1003 762 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5494 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2031 3225 4183 Size: 416 53 83 399 24 614 1194 958 Cuts at: 4183 442 Size: 1753 Fragments arranged by size: 1753 1194 958 614 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2178 2672 4154 1239 Size: 939 494 1482 2579 Fragments arranged by size: 2579 1482 939 494 Bpu10I CC'TnA_GC Cuts at: 2194 2194 Size: 5494 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2405 2549 3619 4484 4782 2405 Size: 144 1070 865 298 3117 Fragments arranged by size: 3117 1070 865 298 144 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4157 882 Size: 617 2658 2219 Fragments arranged by size: 2658 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3285 493 Size: 711 2081 2702 Fragments arranged by size: 2702 2081 711 BsaBI GATnn'nnATC Cuts at: 5488 5488 Size: 5494 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3301 3683 4318 275 Size: 53 83 186 936 1768 382 635 1451 Fragments arranged by size: 1768 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3927 4325 4599 4746 5157 3927 Size: 398 274 147 411 4264 Fragments arranged by size: 4264 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5269 Fragments arranged by size: 5269 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2294 2393 2402 1186 Size: 1108 99 9 4278 Fragments arranged by size: 4278 1108 99 9 BseYI C'CCAG_C Cuts at: 2329 4697 5193 2329 Size: 2368 496 2630 Fragments arranged by size: 2630 2368 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4921 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2269 2371 2944 3705 4733 1540 Size: 275 186 268 102 573 761 1028 2301 Fragments arranged by size: 2301 1028 761 573 275 268 186 102 BsiHKAI G_wGCw'C Cuts at: 2421 2487 3555 3640 4711 2421 Size: 66 1068 85 1071 3204 Fragments arranged by size: 3204 1071 1068 85 66 BsmI GAATG_Cn' Cuts at: 3005 3098 3005 Size: 93 5401 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2421 2487 2793 2813 3555 3640 4711 Size: 399 631 66 306 20 742 85 1071 Cuts at: 4711 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 631 399 306 85 66 20 BspEI T'CCGG_A Cuts at: 5157 5157 Size: 5494 BspHI T'CATG_A Cuts at: 3383 5113 3383 Size: 1730 3764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2849 844 Size: 2005 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3381 1394 Size: 429 1558 3507 Fragments arranged by size: 3507 1558 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3989 4163 65 Size: 1183 2741 174 1396 Fragments arranged by size: 2741 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4138 4325 1700 Size: 110 2328 187 2869 Fragments arranged by size: 2869 2328 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5494 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5494 BssSI C'ACGA_G Cuts at: 3554 4566 3554 Size: 1012 4482 BstAPI GCAn_nnn'nTGC Cuts at: 794 2050 794 Size: 1256 4238 BstBI TT'CG_AA Cuts at: 4298 4298 Size: 5494 BstEII G'GTnAC_C Cuts at: 1184 2584 1184 Size: 1400 4094 BstYI r'GATC_y Cuts at: 1972 2824 3191 3576 3593 5045 5131 5143 5489 Size: 852 367 385 17 1452 86 12 346 Cuts at: 5489 1972 Size: 1977 Fragments arranged by size: 1977 1452 852 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2499 513 Size: 552 1434 3508 Fragments arranged by size: 3508 1434 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2442 487 Size: 683 56 1216 3539 Fragments arranged by size: 3539 1216 683 56 BtsI GCAGTG_nn' Cuts at: 2558 3010 3804 3824 2558 Size: 452 794 20 4228 Fragments arranged by size: 4228 794 452 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5494 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2554 2589 529 Size: 35 1990 35 3434 Fragments arranged by size: 3434 1990 35 35 DraI TTT'AAA Cuts at: 2798 2798 Size: 5494 DraIII CAC_nnn'GTG Cuts at: 2584 2584 Size: 5494 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4501 809 Size: 507 3185 1802 Fragments arranged by size: 3185 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2368 2496 2575 Size: 54 1425 50 275 18 538 128 79 Cuts at: 2575 2941 3830 5459 8 Size: 366 889 1629 43 Fragments arranged by size: 1629 1425 889 538 366 275 128 79 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2368 2941 1537 Size: 275 556 573 4090 Fragments arranged by size: 4090 573 556 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3424 1353 Size: 2071 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4081 4455 4601 387 Size: 1064 138 2492 374 146 1280 Fragments arranged by size: 2492 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2178 2318 2672 3557 4154 4941 Size: 393 506 40 140 354 885 597 787 Cuts at: 4941 5209 1239 Size: 268 1524 Fragments arranged by size: 1524 885 787 597 506 393 354 268 140 40 EcoO109I rG'GnC_Cy Cuts at: 2198 2313 2198 Size: 115 5379 EcoRI G'AATT_C Cuts at: 2803 3181 2803 Size: 378 5116 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2032 2143 2032 Size: 111 5383 FspI TGC'GCA Cuts at: 4000 4000 Size: 5494 HaeII r_GCGC'y Cuts at: 1678 4641 1678 Size: 2963 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2209 4135 4167 4209 Size: 32 135 32 605 32 1926 32 42 Cuts at: 4209 4241 1373 Size: 32 2626 Fragments arranged by size: 2626 1926 605 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2838 3084 669 Size: 988 281 900 246 3079 Fragments arranged by size: 3079 988 900 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5494 HpaI GTT'AAC Cuts at: 3084 3084 Size: 5494 KpnI G_GTAC'C Cuts at: 2819 2819 Size: 5494 MfeI C'AATT_G Cuts at: 3093 3093 Size: 5494 MluI A'CGCG_T Cuts at: 5370 5370 Size: 5494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2280 4608 4792 1305 Size: 975 2328 184 2007 Fragments arranged by size: 2328 2007 975 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2498 2577 10 Size: 54 1425 343 666 79 2927 Fragments arranged by size: 2927 1425 666 343 79 54 MslI CAynn'nnrTG Cuts at: 518 1166 3452 3811 3970 518 Size: 648 2286 359 159 2042 Fragments arranged by size: 2286 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2333 2367 2405 2449 2910 3587 4735 4980 Size: 727 34 38 44 461 677 1148 245 Cuts at: 4980 1606 Size: 2120 Fragments arranged by size: 2120 1148 727 677 461 245 44 38 34 NcoI C'CATG_G Cuts at: 513 1065 2499 513 Size: 552 1434 3508 Fragments arranged by size: 3508 1434 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5494 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5494 NotI GC'GGCC_GC Cuts at: 2941 2941 Size: 5494 NspI r_CATG'y Cuts at: 1132 1229 2852 4397 1132 Size: 97 1623 1545 2229 Fragments arranged by size: 2229 1623 1545 97 PasI CC'CwG_GG Cuts at: 1348 2055 1348 Size: 707 4787 PciI A'CATG_T Cuts at: 4393 4393 Size: 5494 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5494 PfoI T'CCnGG_A Cuts at: 2679 2679 Size: 5494 PmeI GTTT'AAAC Cuts at: 2798 2798 Size: 5494 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3563 3595 5076 5108 3563 Size: 32 1481 32 3949 Fragments arranged by size: 3949 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2198 2198 Size: 5494 PshAI GACnn'nnGTC Cuts at: 1613 2591 1613 Size: 978 4516 PsiI TTA'TAA Cuts at: 3064 3064 Size: 5494 PspOMI G'GGCC_C Cuts at: 2789 2789 Size: 5494 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2694 2726 2694 Size: 32 5462 PstI C_TGCA'G Cuts at: 830 2846 830 Size: 2016 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5494 SalI G'TCGA_C Cuts at: 1936 2836 1936 Size: 900 4594 SbfI CC_TGCA'GG Cuts at: 2846 2846 Size: 5494 ScaI AGT'ACT Cuts at: 3742 5454 3742 Size: 1712 3782 SexAI A'CCwGG_T Cuts at: 2580 2580 Size: 5494 SfcI C'TryA_G Cuts at: 826 1045 2077 2515 2842 3977 4658 4849 826 Size: 219 1032 438 327 1135 681 191 1471 Fragments arranged by size: 1471 1135 1032 681 438 327 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2160 2160 Size: 5494 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5494 SmaI CCC'GGG Cuts at: 1872 2821 1872 Size: 949 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2420 2564 3598 4499 4761 820 Size: 197 926 477 144 1034 901 262 1553 Fragments arranged by size: 1553 1034 926 901 477 262 197 144 SnaBI TAC'GTA Cuts at: 493 3285 493 Size: 2792 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5494 SphI G_CATG'C Cuts at: 1132 2852 1132 Size: 1720 3774 SspI AAT'ATT Cuts at: 5 52 3418 5 Size: 47 3366 2081 Fragments arranged by size: 3366 2081 47 StuI AGG'CCT Cuts at: 1517 2163 1517 Size: 646 4848 StyI C'CwwG_G Cuts at: 513 1065 2167 2499 2933 513 Size: 552 1102 332 434 3074 Fragments arranged by size: 3074 1102 552 434 332 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3521 3538 3691 3876 4321 325 Size: 1255 1941 17 153 185 445 1498 Fragments arranged by size: 1941 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3740 5452 96 Size: 274 80 33 51 1443 1763 1712 138 Fragments arranged by size: 1763 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5454 5486 5454 Size: 32 5462 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5494 XbaI T'CTAG_A Cuts at: 2830 2830 Size: 5494 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2062 2062 Size: 5494 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5494 XmaI C'CCGG_G Cuts at: 1870 2819 1870 Size: 949 4545 XmnI GAAnn'nnTTC Cuts at: 2045 3623 2045 Size: 1578 3916 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3302 276 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PvuII RsrII SacI SacII SanDI SapI SfoI SrfI SwaI