################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI AlwNI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BclI BfaI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaI BsaWI BseAI BseRI BsgI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII Eam1105I EarI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT14I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MaeII MaeIII MfeI MluI MluNI MroI MscI MslI MunI NaeI NcoI NdeI NgoMIV NheI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcsI 493 AAATTT [GA]^AATT[CT] AflIII 458 ACATGT A^C[GA][CT]GT AluI 441 AGCT AG^CT Alw44I 349 GTGCAC G^TGCAC AlwI 63 GGATC GGATC(4/5) AocI 361 CCTGAGG CC^T[ACGT]AGG ApaI 278 GGGCCC GGGCC^C ApaLI 349 GTGCAC G^TGCAC ApoI 493 AAATTT [GA]^AATT[CT] BciVI 499 GTATCC GTATCC(6/5) BglII 700 AGATCT A^GATCT BsaBI 517 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 329 CGTACG C^GTACG BspLU11I 458 ACATGT A^CATGT BsrI 74 CCAGT ACTGG(1/-1) BstNI 420 CCTGG CC^[AT]GG Bsu36I 361 CCTGAGG CC^T[ACGT]AGG DraII 277 GGGGCCC [GA]G^G[ACGT]CC[CT] DrdI 641 GACTGCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 361 CCTGAGG CC^T[ACGT]AGG EcoO109I 277 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoRII 420 CCTGG ^CC[AT]GG EcoT22I 654 ATGCAT ATGCA^T HgaI 271 GCGTC GACGC(5/10) HinfI 343 GACTC G^A[ACGT]TC MamI 517 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 610 GAAGA GAAGA(8/7) MvaI 420 CCTGG CC^[AT]GG NlaIII 459 CATG CATG^ NotI 199 GCGGCCGC GC^GGCCGC NsiI 654 ATGCAT ATGCA^T NspI 458 ACATGT [GA]CATG^[CT] PleI 343 GACTC GAGTC(4/5) SfcI 632 CTATAG C^T[GA][CT]AG SfiI 201 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AspHI 349 GTGCAC G[AT]GC[AT]^C 712 GAGCAC AvaI 54 CTCGGG C^[CT]CG[GA]G 122 CTCGGG AvaII 98 GGACC G^G[AT]CC 384 GGACC BsiHKAI 349 GTGCAC G[AT]GC[AT]^C 712 GAGCAC BsoBI 54 CTCGGG C^[CT]CG[GA]G 122 CTCGGG BsrBI 240 CCGCTC CCGCTC(-3/-3) 15 GAGCGG BsrFI 386 ACCGGC [GA]^CCGG[CT] 390 GCCGGT BssHII 45 GCGCGC G^CGCGC 164 GCGCGC BstF5I 306 CATCC GGATG(2/0) 567 GGATG BstYI 63 GGATCT [GA]^GATC[CT] 700 AGATCT Cfr10I 386 ACCGGC [GA]^CCGG[CT] 390 GCCGGT FokI 306 CATCC GGATG(9/13) 567 GGATG HphI 423 GGTGA GGTGA(8/7) 444 TCACC MflI 63 GGATCT [GA]^GATC[CT] 700 AGATCT MseI 473 TTAA T^TAA 538 TTAA Tru9I 473 TTAA T^TAA 538 TTAA Tsp509I 494 AATT ^AATT 614 AATT XhoII 63 GGATCT [GA]^GATC[CT] 700 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AcyI AfaI BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BsiEI BsiYI BslI BsmFI Bsp1286I BssKI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EagI EclXI Eco52I FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI KspI MboI MnlI MspA1I MspI MvnI MwoI NarI NciI NdeII NlaIV RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfoI TseI