(Circular) (Six-base) MAPSORT of: pFN21AE4032.seq Check: 9345 from: 1 to: 5431 pFN21AE4032 5431 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3241 278 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 1937 Size: 837 4594 Acc65I G'GTAC_C Cuts at: 2752 2752 Size: 5431 AclI AA'CG_TT Cuts at: 1563 3558 3931 1563 Size: 1995 373 3063 Fragments arranged by size: 3063 1995 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2290 2709 3494 4878 5146 1632 Size: 658 419 785 1384 268 1917 Fragments arranged by size: 1917 1384 785 658 419 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5234 AflIII A'CryG_T Cuts at: 1203 2463 4330 5307 1203 Size: 1260 1867 977 1327 Fragments arranged by size: 1867 1327 1260 977 AgeI A'CCGG_T Cuts at: 4262 4262 Size: 5431 AhdI GACnn_n'nnGTC Cuts at: 4160 4160 Size: 5431 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5431 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5431 AlwNI CAG_nnn'CTG Cuts at: 3174 4746 3174 Size: 1572 3859 ApaI G_GGCC'C Cuts at: 2287 2287 Size: 5431 ApaLI G'TGCA_C Cuts at: 2354 3488 4644 2354 Size: 1134 1156 3141 Fragments arranged by size: 3141 1156 1134 ApoI r'AATT_y Cuts at: 1493 2498 2740 2966 3118 5227 1493 Size: 1005 242 226 152 2109 1697 Fragments arranged by size: 2109 1697 1005 242 226 152 AseI AT'TA_AT Cuts at: 160 1032 3985 160 Size: 872 2953 1606 Fragments arranged by size: 2953 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 AvaI C'yCGr_G Cuts at: 1870 1943 2059 2127 2756 1870 Size: 73 116 68 629 4545 Fragments arranged by size: 4545 629 116 73 68 BamHI G'GATC_C Cuts at: 2761 3128 2761 Size: 367 5064 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 2093 2161 2195 2752 Size: 325 217 627 222 84 68 34 557 Cuts at: 2752 4207 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 557 325 222 217 84 68 34 BanII G_rGCy'C Cuts at: 1391 2066 2287 2411 2750 1391 Size: 675 221 124 339 4072 Fragments arranged by size: 4072 675 339 221 124 BbeI G_GCGC'C Cuts at: 2013 2097 2199 2013 Size: 84 102 5245 Fragments arranged by size: 5245 102 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4460 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3622 3656 1903 Size: 34 1685 34 3678 Fragments arranged by size: 3678 1685 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2515 3323 4533 2515 Size: 808 1210 3413 Fragments arranged by size: 3413 1210 808 BclI T'GATC_A Cuts at: 1548 1548 Size: 5431 BfrBI ATG'CAT Cuts at: 2661 2661 Size: 5431 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2204 2213 2231 Size: 107 122 71 631 605 532 9 18 Cuts at: 2231 2291 2337 4042 136 Size: 60 46 1705 1525 Fragments arranged by size: 1705 1525 631 605 532 122 107 71 60 46 18 9 BglII A'GATC_T Cuts at: 2705 5426 2705 Size: 2721 2710 BlpI GC'TnA_GC Cuts at: 2848 2848 Size: 5431 Bme1580I G_kGCm'C Cuts at: 1790 2166 2287 2358 2486 3492 4648 1790 Size: 376 121 71 128 1006 1156 2573 Fragments arranged by size: 2573 1156 1006 376 128 121 71 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5431 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3162 4120 442 Size: 416 53 83 399 24 1745 958 1753 Fragments arranged by size: 1753 1745 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5431 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4091 1239 Size: 2852 2579 Bpu10I CC'TnA_GC Cuts at: 2157 2715 2157 Size: 558 4873 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3556 4421 4719 3556 Size: 865 298 4268 Fragments arranged by size: 4268 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4094 882 Size: 617 2595 2219 Fragments arranged by size: 2595 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3222 493 Size: 711 2018 2702 Fragments arranged by size: 2702 2018 711 BsaBI GATnn'nnATC Cuts at: 2526 5425 2526 Size: 2899 2532 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 2094 2196 2276 Size: 53 83 186 936 477 84 102 80 Cuts at: 2276 3238 3620 4255 275 Size: 962 382 635 1451 Fragments arranged by size: 1451 962 936 635 477 382 186 102 84 83 80 53 BsaWI w'CCGG_w Cuts at: 3864 4262 4536 4683 5094 3864 Size: 398 274 147 411 4201 Fragments arranged by size: 4201 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5206 Fragments arranged by size: 5206 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5431 BseYI C'CCAG_C Cuts at: 2165 2180 2591 4634 5130 2165 Size: 15 411 2043 496 2466 Fragments arranged by size: 2466 2043 496 411 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4858 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2031 2044 2208 2386 2396 2881 Size: 275 186 30 13 164 178 10 485 Cuts at: 2881 3642 4670 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 485 275 186 178 164 30 13 10 BsiHKAI G_wGCw'C Cuts at: 2358 2721 3492 3577 4648 2358 Size: 363 771 85 1071 3141 Fragments arranged by size: 3141 1071 771 363 85 BsiWI C'GTAC_G Cuts at: 2334 2334 Size: 5431 BsmI GAATG_Cn' Cuts at: 2942 3035 2942 Size: 93 5338 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2166 2287 2358 2411 2486 2721 Size: 399 276 100 121 71 53 75 235 Cuts at: 2721 2750 3492 3577 4648 1391 Size: 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 276 235 121 100 85 75 71 53 29 BspEI T'CCGG_A Cuts at: 5094 5094 Size: 5431 BspHI T'CATG_A Cuts at: 3320 5050 3320 Size: 1730 3701 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2786 844 Size: 1942 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2022 2247 3318 1394 Size: 429 199 225 1071 3507 Fragments arranged by size: 3507 1071 429 225 199 BsrDI GCAATG_nn' Cuts at: 65 1248 3926 4100 65 Size: 1183 2678 174 1396 Fragments arranged by size: 2678 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2391 2395 4075 4262 1700 Size: 110 581 4 1680 187 2869 Fragments arranged by size: 2869 1680 581 187 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5431 BssHII G'CGCG_C Cuts at: 1925 2050 2169 1925 Size: 125 119 5187 Fragments arranged by size: 5187 125 119 BssSI C'ACGA_G Cuts at: 3491 4503 3491 Size: 1012 4419 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5431 BstBI TT'CG_AA Cuts at: 4235 4235 Size: 5431 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5431 BstYI r'GATC_y Cuts at: 1972 2068 2705 2761 3128 3513 3530 4982 5068 Size: 96 637 56 367 385 17 1452 86 Cuts at: 5068 5080 5426 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 637 385 367 346 96 86 56 17 12 Bsu36I CC'TnA_GG Cuts at: 2367 2367 Size: 5431 BtgI C'CryG_G Cuts at: 513 1065 2004 2106 2139 2199 2208 2262 513 Size: 552 939 102 33 60 9 54 3682 Fragments arranged by size: 3682 939 552 102 60 54 33 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4692 Fragments arranged by size: 4692 683 56 BtsI GCAGTG_nn' Cuts at: 2947 3741 3761 2947 Size: 794 20 4617 Fragments arranged by size: 4617 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5431 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5396 DraI TTT'AAA Cuts at: 2735 2735 Size: 5431 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2652 4438 809 Size: 507 1336 1786 1802 Fragments arranged by size: 1802 1786 1336 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2041 2205 Size: 54 1425 50 275 18 198 13 164 Cuts at: 2205 2383 2393 2878 3767 5396 8 Size: 178 10 485 889 1629 43 Fragments arranged by size: 1629 1425 889 485 275 198 178 164 54 50 43 18 13 10 EagI C'GGCC_G Cuts at: 1537 1812 2028 2041 2205 2383 2393 2878 1537 Size: 275 216 13 164 178 10 485 4090 Fragments arranged by size: 4090 485 275 216 178 164 13 10 EarI CTCTTCn'nnn_ Cuts at: 1353 3361 1353 Size: 2008 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4018 4392 4538 387 Size: 1064 138 2429 374 146 1280 Fragments arranged by size: 2429 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2290 2709 3494 4091 4878 5146 1239 Size: 393 658 419 785 597 787 268 1524 Fragments arranged by size: 1524 787 785 658 597 419 393 268 EcoO109I rG'GnC_Cy Cuts at: 2283 2283 Size: 5431 EcoRI G'AATT_C Cuts at: 2740 3118 2740 Size: 378 5053 FspI TGC'GCA Cuts at: 3937 3937 Size: 5431 HaeII r_GCGC'y Cuts at: 1678 2013 2097 2127 2187 2199 4578 1678 Size: 335 84 30 60 12 2379 2531 Fragments arranged by size: 2531 2379 335 84 60 30 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4072 4104 4146 4178 1373 Size: 32 135 32 2500 32 42 32 2626 Fragments arranged by size: 2626 2500 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2775 3021 669 Size: 988 281 837 246 3079 Fragments arranged by size: 3079 988 837 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5431 HpaI GTT'AAC Cuts at: 3021 3021 Size: 5431 KasI G'GCGC_C Cuts at: 2009 2093 2195 2009 Size: 84 102 5245 Fragments arranged by size: 5245 102 84 KpnI G_GTAC'C Cuts at: 2756 2756 Size: 5431 MfeI C'AATT_G Cuts at: 3030 3030 Size: 5431 MluI A'CGCG_T Cuts at: 5307 5307 Size: 5431 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4545 4729 1305 Size: 3240 184 2007 Fragments arranged by size: 3240 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3609 Fragments arranged by size: 3609 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3389 3748 3907 518 Size: 648 2223 359 159 2042 Fragments arranged by size: 2223 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2088 2108 2141 2201 2210 2328 2847 3524 Size: 482 20 33 60 9 118 519 677 Cuts at: 3524 4672 4917 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 519 482 245 118 60 33 20 9 NarI GG'CG_CC Cuts at: 2010 2094 2196 2010 Size: 84 102 5245 Fragments arranged by size: 5245 102 84 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3940 Fragments arranged by size: 3940 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5431 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5431 NotI GC'GGCC_GC Cuts at: 2205 2878 2205 Size: 673 4758 NsiI A_TGCA'T Cuts at: 2663 2663 Size: 5431 NspI r_CATG'y Cuts at: 1132 1229 2467 2789 4334 1132 Size: 97 1238 322 1545 2229 Fragments arranged by size: 2229 1545 1238 322 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5431 PciI A'CATG_T Cuts at: 2463 4330 2463 Size: 1867 3564 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5431 PmeI GTTT'AAAC Cuts at: 2735 2735 Size: 5431 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3500 3532 5013 5045 3500 Size: 32 1481 32 3886 Fragments arranged by size: 3886 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5431 PsiI TTA'TAA Cuts at: 3001 3001 Size: 5431 PspOMI G'GGCC_C Cuts at: 2283 2283 Size: 5431 PstI C_TGCA'G Cuts at: 830 2783 830 Size: 1953 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5431 SacII CC_GC'GG Cuts at: 2109 2142 2202 2211 2109 Size: 33 60 9 5329 Fragments arranged by size: 5329 60 33 9 SalI G'TCGA_C Cuts at: 1936 2773 1936 Size: 837 4594 SbfI CC_TGCA'GG Cuts at: 2783 2783 Size: 5431 ScaI AGT'ACT Cuts at: 3679 5391 3679 Size: 1712 3719 SfcI C'TryA_G Cuts at: 826 1045 2637 2779 3914 4595 4786 826 Size: 219 1592 142 1135 681 191 1471 Fragments arranged by size: 1592 1471 1135 681 219 191 142 SfiI GGCCn_nnn'nGGCC Cuts at: 2213 2213 Size: 5431 SfoI GGC'GCC Cuts at: 2011 2095 2197 2011 Size: 84 102 5245 Fragments arranged by size: 5245 102 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5431 SmaI CCC'GGG Cuts at: 1872 2758 1872 Size: 886 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3535 4436 4698 820 Size: 197 926 1592 901 262 1553 Fragments arranged by size: 1592 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3222 493 Size: 2729 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5431 SphI G_CATG'C Cuts at: 1132 2789 1132 Size: 1657 3774 SspI AAT'ATT Cuts at: 5 52 3355 5 Size: 47 3303 2081 Fragments arranged by size: 3303 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5431 StyI C'CwwG_G Cuts at: 513 1065 2004 2870 513 Size: 552 939 866 3074 Fragments arranged by size: 3074 939 866 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3458 3475 3628 3813 4258 325 Size: 1255 1878 17 153 185 445 1498 Fragments arranged by size: 1878 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2575 3677 5389 Size: 274 80 33 51 1443 598 1102 1712 Cuts at: 5389 96 Size: 138 Fragments arranged by size: 1712 1443 1102 598 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5391 5423 5391 Size: 32 5399 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5431 XbaI T'CTAG_A Cuts at: 2767 2767 Size: 5431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5431 XmaI C'CCGG_G Cuts at: 1870 2756 1870 Size: 886 4545 XmnI GAAnn'nnTTC Cuts at: 3560 3560 Size: 5431 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3239 276 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI