################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AclI AflII AgeI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BpuAI BsaBI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraIII DrdI EarI Eco47III Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I MamI MboI MfeI MflI MluI MseI MunI NcoI NdeI NdeII NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfuI SmlI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tth111I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 301 GTCGAC GT^[AC][GT]AC AccIII 313 TCCGGA T^CCGGA AfaI 411 GTAC GT^AC AflIII 923 ACACGT A^C[GA][CT]GT AhdI 340 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 721 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1000 GGGCCC GGGCC^C Asp700I 597 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 340 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1162 GTGCTC G[AT]GC[AT]^C BalI 728 TGGCCA TGG^CCA BciVI 622 GTATCC GTATCC(6/5) BfaI 1217 CTAG C^TAG BlpI 1164 GCTCAGC GC^T[ACGT]AGC BpmI 592 CTGGAG CTGGAG(16/14) Bpu1102I 1164 GCTCAGC GC^T[ACGT]AGC BsaAI 619 TACGTA [CT]AC^GT[GA] BsaI 472 GAGACC GGTCTC(1/5) BsaWI 313 TCCGGA [AT]^CCGG[AT] BseAI 313 TCCGGA T^CCGGA BsiHKAI 1162 GTGCTC G[AT]GC[AT]^C BsmFI 157 GTCCC GGGAC(10/14) BspEI 313 TCCGGA T^CCGGA CelII 1164 GCTCAGC GC^T[ACGT]AGC DraII 282 AGGACCC [GA]G^G[ACGT]CC[CT] Eam1105I 340 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 282 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 601 GAATTC G^AATTC HgaI 549 GCGTC GACGC(5/10) HincII 301 GTCGAC GT[CT]^[GA]AC HindII 301 GTCGAC GT[CT]^[GA]AC MaeI 1217 CTAG C^TAG MboII 361 GAAGA GAAGA(8/7) MluNI 728 TGGCCA TGG^CCA MroI 313 TCCGGA T^CCGGA MscI 728 TGGCCA TGG^CCA NaeI 1027 GCCGGC GCC^GGC NgoMIV 1027 GCCGGC G^CCGGC PflMI 731 CCATGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 282 AGGACCC [GA]G^G[AT]CC[CT] RsaI 411 GTAC GT^AC SalI 301 GTCGAC G^TCGAC SgrAI 848 CGCCGGTG C[GA]^CCGG[CT]G SnaBI 619 TACGTA TAC^GTA StuI 1172 AGGCCT AGG^CCT Van91I 731 CCATGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 597 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 75 AAATTT [GA]^AATT[CT] 601 GAATTC ApoI 75 AAATTT [GA]^AATT[CT] 601 GAATTC BsgI 378 CTGCAC GTGCAG(16/14) 528 GTGCAG BsiEI 650 CGGCCG CG[GA][CT]^CG 791 CGGCCG BsmAI 472 GAGAC GTCTC(1/5) 70 GTCTC BspMI 662 ACCTGC ACCTGC(4/8) 827 ACCTGC BstF5I 869 CATCC GGATG(2/0) 33 GGATG BstXI 252 CCAGGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1070 CCAACGGGCTGG EagI 650 CGGCCG C^GGCCG 791 CGGCCG EclXI 650 CGGCCG C^GGCCG 791 CGGCCG Eco52I 650 CGGCCG C^GGCCG 791 CGGCCG FokI 869 CATCC GGATG(9/13) 33 GGATG NotI 649 GCGGCCGC GC^GGCCGC 790 GCGGCCGC SfaNI 546 GATGC GCATC(5/9) 868 GCATC Tsp45I 539 GTGAC ^GT[GC]AC 1055 GTCAC Tsp509I 76 AATT ^AATT 602 AATT TspRI 495 CAGTG CA[GC]TG[ACGT][ACGT]^ 537 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcyI AluI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BseRI BsiYI BslI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DsaI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeII MaeIII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV PleI SacII Sau96I ScrFI SfoI SmaI TaqI TseI TthHB8I XmaCI XmaI