Restriction map for
pFN21AB9721
(Circular) (Six-base) MAPSORT of: pFN21AB9721.seq Check: 6214 from: 1 to: 5926
pFN21AB9721 5926 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2841 2841
Size: 5926
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2307 3269 1937
Size: 370 962 4594
Fragments arranged by size:
4594 962 370
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2887 3989 5373 5641 1632
Size: 1255 1102 1384 268 1917
Fragments arranged by size:
1917 1384 1255 1102 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 2928 4825 5802 1203
Size: 1725 1897 977 1327
Fragments arranged by size:
1897 1725 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 2350 4655 2350
Size: 2305 3621
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2123 1184
Size: 939 4987
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2612 2644 2612
Size: 32 5894
AlwNI CAG_nnn'CTG
Cuts at: 2731 3669 5241 2731
Size: 938 1572 3416
Fragments arranged by size:
3416 1572 938
ApaI G_GGCC'C
Cuts at: 3009 3009
Size: 5926
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 2080 2606 3235 3461 3613 5722 1493
Size: 587 526 629 226 152 2109 1697
Fragments arranged by size:
2109 1697 629 587 526 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 2154 2175 2577 3002 3090 3251
Size: 73 177 34 21 402 425 88 161
Cuts at: 3251 1870
Size: 4545
Fragments arranged by size:
4545 425 402 177 161 88 73 34
21
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2131 2246 2522 2867 3023
Size: 325 217 627 344 115 276 345 156
Cuts at: 3023 3085 3211 3247 4702 618
Size: 62 126 36 1455 1842
Fragments arranged by size:
1842 1455 627 345 344 325 276 217
156 126 115 62 36
BanII G_rGCy'C
Cuts at: 1391 2153 2256 3009 3245 1391
Size: 762 103 753 236 4072
Fragments arranged by size:
4072 762 753 236 103
BbeI G_GCGC'C
Cuts at: 2250 2871 3027 3089 2250
Size: 621 156 62 5087
Fragments arranged by size:
5087 621 156 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4955
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4117 4151 1903
Size: 34 2180 34 3678
Fragments arranged by size:
3678 2180 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2638 3818 5028 2638
Size: 1180 1210 3536
Fragments arranged by size:
3536 1210 1180
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4537 136
Size: 107 122 71 631 605 2865 1525
Fragments arranged by size:
2865 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5921 5921
Size: 5926
BlpI GC'TnA_GC
Cuts at: 3170 3343 3170
Size: 173 5753
Bme1580I G_kGCm'C
Cuts at: 1790 2136 3009 3216 3987 5143 1790
Size: 346 873 207 771 1156 2573
Fragments arranged by size:
2573 1156 873 771 346 207
BmgBI CAC'GTC
Cuts at: 1318 2742 1318
Size: 1424 4502
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2328 3029 3657
Size: 416 53 83 399 24 911 701 628
Cuts at: 3657 4615 442
Size: 958 1753
Fragments arranged by size:
1753 958 911 701 628 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2618 4586 1239
Size: 1379 1968 2579
Fragments arranged by size:
2579 1968 1379
Bpu10I CC'TnA_GC
Cuts at: 2147 2147
Size: 5926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4051 4916 5214 4051
Size: 865 298 4763
Fragments arranged by size:
4763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2471 4589 882
Size: 617 972 2118 2219
Fragments arranged by size:
2219 2118 972 617
BsaAI yAC'GTr
Cuts at: 493 1204 2626 3717 493
Size: 711 1422 1091 2702
Fragments arranged by size:
2702 1422 1091 711
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2247 2868 3024 3086
Size: 53 83 186 936 714 621 156 62
Cuts at: 3086 3733 4115 4750 275
Size: 647 382 635 1451
Fragments arranged by size:
1451 936 714 647 635 621 382 186
156 83 62 53
BsaWI w'CCGG_w
Cuts at: 2318 4359 4757 5031 5178 5589 2318
Size: 2041 398 274 147 411 2655
Fragments arranged by size:
2655 2041 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 1178
Size: 30 165 30 897 30 4774
Fragments arranged by size:
4774 897 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2216 2219 3172 3221 1186
Size: 1030 3 953 49 3891
Fragments arranged by size:
3891 1030 953 49 3
BseYI C'CCAG_C
Cuts at: 2264 5129 5625 2264
Size: 2865 496 2565
Fragments arranged by size:
2865 2565 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2368 2554 1163
Size: 573 632 186 4535
Fragments arranged by size:
4535 632 573 186
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2658 2799 3376 4137 5165 1540
Size: 275 186 657 141 577 761 1028 2301
Fragments arranged by size:
2301 1028 761 657 577 275 186 141
BsiHKAI G_wGCw'C
Cuts at: 3171 3987 4072 5143 3171
Size: 816 85 1071 3954
Fragments arranged by size:
3954 1071 816 85
BsmI GAATG_Cn'
Cuts at: 3437 3530 3437
Size: 93 5833
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2153 2256 3009 3171 3216 3245
Size: 399 346 17 103 753 162 45 29
Cuts at: 3245 3987 4072 5143 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 753 742 399 346 162 103
85 45 29 17
BspEI T'CCGG_A
Cuts at: 2318 5589 2318
Size: 3271 2655
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2676 2841 3281 844
Size: 1832 165 440 3489
Fragments arranged by size:
3489 1832 440 165
BsrBI CCG'CTC
Cuts at: 1394 1823 2923 2995 3135 3813 1394
Size: 429 1100 72 140 678 3507
Fragments arranged by size:
3507 1100 678 429 140 72
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 4595 65
Size: 1183 3173 174 1396
Fragments arranged by size:
3173 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2209 2703 2854 3032 4570 4757 1700
Size: 110 399 494 151 178 1538 187 2869
Fragments arranged by size:
2869 1538 494 399 187 178 151 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5926
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstXI CCAn_nnnn'nTGG
Cuts at: 2264 3082 2264
Size: 818 5108
BstYI r'GATC_y
Cuts at: 1972 3256 3623 4008 4025 5477 5563 5575 5921
Size: 1284 367 385 17 1452 86 12 346
Cuts at: 5921 1972
Size: 1977
Fragments arranged by size:
1977 1452 1284 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2094 2137 2636 2799 2957 513
Size: 552 939 90 43 499 163 158 3482
Fragments arranged by size:
3482 939 552 499 163 158 90 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2811 487
Size: 683 56 1585 3602
Fragments arranged by size:
3602 1585 683 56
BtsI GCAGTG_nn'
Cuts at: 2506 3442 4236 4256 2506
Size: 936 794 20 4176
Fragments arranged by size:
4176 936 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4933 809
Size: 507 3617 1802
Fragments arranged by size:
3617 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2169 2271 2655
Size: 54 1425 50 275 18 339 102 384
Cuts at: 2655 2733 2744 2796 3072 3373 4262 5891 8
Size: 78 11 52 276 301 889 1629 43
Fragments arranged by size:
1629 1425 889 384 339 301 276 275
102 78 54 52 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2655 2796 3373 1537
Size: 275 843 141 577 4090
Fragments arranged by size:
4090 843 577 275 141
EarI CTCTTCn'nnn_
Cuts at: 1353 3856 1353
Size: 2503 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2771 2866 2875 2972 4513 4887
Size: 1064 138 1182 95 9 97 1541 374
Cuts at: 4887 5033 387
Size: 146 1280
Fragments arranged by size:
1541 1280 1182 1064 374 146 138 97
95 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2618 2887 3989 4586 5373 5641 1239
Size: 393 986 269 1102 597 787 268 1524
Fragments arranged by size:
1524 1102 986 787 597 393 269 268
EcoO109I rG'GnC_Cy
Cuts at: 2288 2288
Size: 5926
EcoRI G'AATT_C
Cuts at: 2606 3235 3613 2606
Size: 629 378 4919
Fragments arranged by size:
4919 629 378
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 2250 2450 2541 2731 2871 3027 3089 5073
Size: 572 200 91 190 140 156 62 1984
Cuts at: 5073 1678
Size: 2531
Fragments arranged by size:
2531 1984 572 200 190 156 140 91
62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 2336 2368 4567
Size: 32 135 32 728 32 4 32 2199
Cuts at: 4567 4599 4641 4673 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2199 728 135 42 32 32 32
32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2308 3270 3516 669
Size: 988 281 370 962 246 3079
Fragments arranged by size:
3079 988 962 370 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KasI G'GCGC_C
Cuts at: 2246 2867 3023 3085 2246
Size: 621 156 62 5087
Fragments arranged by size:
5087 621 156 62
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2334 5040 5224 1305
Size: 1029 2706 184 2007
Fragments arranged by size:
2706 2007 1029 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2735 10
Size: 54 1425 343 903 3201
Fragments arranged by size:
3201 1425 903 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2687 2777 3884 4243 4402 518
Size: 648 861 660 90 1107 359 159 2042
Fragments arranged by size:
2042 1107 861 660 648 359 159 90
MspA1I CmG'CkG
Cuts at: 1606 2107 2638 2801 2907 2959 3066 3342 4019
Size: 501 531 163 106 52 107 276 677
Cuts at: 4019 5167 5412 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 531 501 276 245 163
107 106 52
NaeI GCC'GGC
Cuts at: 3034 3034
Size: 5926
NarI GG'CG_CC
Cuts at: 2247 2868 3024 3086 2247
Size: 621 156 62 5087
Fragments arranged by size:
5087 621 156 62
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4435
Fragments arranged by size:
4435 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NgoMIV G'CCGG_C
Cuts at: 3032 3032
Size: 5926
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 2655 2796 3373 2655
Size: 141 577 5208
Fragments arranged by size:
5208 577 141
NspI r_CATG'y
Cuts at: 1132 1229 3284 4829 1132
Size: 97 2055 1545 2229
Fragments arranged by size:
2229 2055 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5926
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2742 1108
Size: 1634 4292
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2288 2288
Size: 5926
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PspOMI G'GGCC_C
Cuts at: 3005 3005
Size: 5926
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2399 2431 2399
Size: 32 5894
PstI C_TGCA'G
Cuts at: 830 3278 830
Size: 2448 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
SacII CC_GC'GG
Cuts at: 2639 2802 2960 2639
Size: 163 158 5605
Fragments arranged by size:
5605 163 158
SalI G'TCGA_C
Cuts at: 1936 2306 3268 1936
Size: 370 962 4594
Fragments arranged by size:
4594 962 370
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SfcI C'TryA_G
Cuts at: 826 1045 3274 4409 5090 5281 826
Size: 219 2229 1135 681 191 1471
Fragments arranged by size:
2229 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2248 2869 3025 3087 2248
Size: 621 156 62 5087
Fragments arranged by size:
5087 621 156 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 2854 1700
Size: 1154 4772
SmaI CCC'GGG
Cuts at: 1872 2177 3004 3092 3253 1872
Size: 305 827 88 161 4545
Fragments arranged by size:
4545 827 305 161 88
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4030 4931 5193 820
Size: 197 926 2087 901 262 1553
Fragments arranged by size:
2087 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2626 3717 493
Size: 2133 1091 2702
Fragments arranged by size:
2702 2133 1091
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 3284 1132
Size: 2152 3774
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 3179 1517
Size: 1662 4264
StyI C'CwwG_G
Cuts at: 513 1065 2004 3365 513
Size: 552 939 1361 3074
Fragments arranged by size:
3074 1361 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3953 3970 4123 4308 4753 325
Size: 1255 2373 17 153 185 445 1498
Fragments arranged by size:
2373 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4172 5884 96
Size: 274 80 33 51 1443 2195 1712 138
Fragments arranged by size:
2195 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5886 5918 5886
Size: 32 5894
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 2175 3002 3090 3251 1870
Size: 305 827 88 161 4545
Fragments arranged by size:
4545 827 305 161 88
XmnI GAAnn'nnTTC
Cuts at: 2606 4055 2606
Size: 1449 4477
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PspXI
PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI
SwaI XcmI