################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BbeI BbsI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MslI MspA1I MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tth111I TthHB8I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcsI 651 AAATTT [GA]^AATT[CT] AflII 81 CTTAAG C^TTAAG ApoI 651 AAATTT [GA]^AATT[CT] Asp700I 16 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 162 GGATCC G^GATCC BanII 317 GGGCTC G[GA]GC[CT]^C BbrPI 635 CACGTG CAC^GTG BbvCI 217 GCTGAGG CCTCAGC(-5/-2) BcnI 778 CCCGG CC^[GC]GG BfrI 81 CTTAAG C^TTAAG BmyI 317 GGGCTC G[AGT]GC[ACT]^C BpmI 752 CTGGAG CTGGAG(16/14) BsaAI 635 CACGTG [CT]AC^GT[GA] BsmFI 984 GGGAC GGGAC(10/14) Bsp1286I 317 GGGCTC G[AGT]GC[ACT]^C Cac8I 169 GCAAGC GC[ACGT]^[ACGT]GC DraIII 635 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG HapII 779 CCGG C^CGG HpaII 779 CCGG C^CGG HphI 378 TCACC GGTGA(8/7) MfeI 254 CAATTG C^AATTG MspI 779 CCGG C^CGG MunI 254 CAATTG C^AATTG NciI 778 CCCGG CC^[GC]GG NdeI 989 CATATG CA^TATG PflMI 988 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 635 CACGTG CAC^GTG PmlI 635 CACGTG CAC^GTG SfaNI 226 GATGC GCATC(5/9) SfcI 287 CTGTAG C^T[GA][CT]AG SmlI 81 CTTAAG C^T[CT][GA]AG Van91I 988 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 565 TCTAGA T^CTAGA XcmI 522 CCATACCATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 16 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 350 GCGG CCGC(-3/-1) 644 GCGG BbvI 229 GCTGC GCAGC(8/12) 238 GCTGC BfaI 566 CTAG C^TAG 596 CTAG BstF5I 209 CATCC GGATG(2/0) 723 GGATG BstYI 162 GGATCC [GA]^GATC[CT] 688 GGATCT DraII 747 AGGACCT [GA]G^G[ACGT]CC[CT] 816 AGGACCT DsaI 212 CCATGG C^C[GA][CT]GG 233 CCATGG EcoO109I 747 AGGACCT [GA]G^G[ACGT]CC[CT] 816 AGGACCT EcoT14I 212 CCATGG C^C[AT][AT]GG 233 CCATGG Fnu4HI 229 GCTGC GC^[ACGT]GC 238 GCTGC FokI 209 CATCC GGATG(9/13) 723 GGATG HindIII 84 AAGCTT A^AGCTT 136 AAGCTT HinfI 301 GAATC G^A[ACGT]TC 631 GATTC ItaI 229 GCTGC GC^[ACGT]GC 238 GCTGC MaeI 566 CTAG C^TAG 596 CTAG MflI 162 GGATCC [GA]^GATC[CT] 688 GGATCT MseI 82 TTAA T^TAA 356 TTAA NcoI 212 CCATGG C^CATGG 233 CCATGG PpuMI 747 AGGACCT [GA]G^G[AT]CC[CT] 816 AGGACCT StyI 212 CCATGG C^C[AT][AT]GG 233 CCATGG TfiI 301 GAATC G^A[AT]TC 631 GATTC Tru9I 82 TTAA T^TAA 356 TTAA TseI 229 GCTGC G^C[AT]GC 238 GCTGC TspRI 705 CAGTG CA[GC]TG[ACGT][ACGT]^ 1020 CAGTG XhoII 162 GGATCC [GA]^GATC[CT] 688 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AluI AlwI AvaII BsaJI BseRI BsiYI BslI BssKI BstNI Cfr13I DdeI DpnII EarI EcoRII Ksp632I MaeII MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI Tsp509I