(Linear) (Six-base) MAPSORT of: KIEE3414.seq Check: 7821 from: 1 to: 1059 KIEE3414.orf With 182 enzymes: SgfI * October 20, 2017 17:04 .. AflII C'TTAA_G Cuts at: 0 81 1059 Size: 81 978 ApoI r'AATT_y Cuts at: 0 651 1059 Size: 651 408 BamHI G'GATC_C Cuts at: 0 162 1059 Size: 162 897 BanII G_rGCy'C Cuts at: 0 321 1059 Size: 321 738 BbvCI CC'TCA_GC Cuts at: 0 218 1059 Size: 218 841 BmgBI CAC'GTC Cuts at: 0 712 1059 Size: 712 347 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 773 1059 Size: 773 286 Bpu10I CC'TnA_GC Cuts at: 0 218 1059 Size: 218 841 BsaAI yAC'GTr Cuts at: 0 637 1059 Size: 637 422 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 623 698 758 803 812 887 1059 Size: 623 75 60 45 9 75 172 Fragments arranged by size: 623 172 75 75 60 45 9 Bsp1286I G_dGCh'C Cuts at: 0 321 1059 Size: 321 738 BstYI r'GATC_y Cuts at: 0 162 688 1059 Size: 162 526 371 Fragments arranged by size: 526 371 162 BtgI C'CryG_G Cuts at: 0 212 233 1059 Size: 212 21 826 Fragments arranged by size: 826 212 21 BtsI GCAGTG_nn' Cuts at: 0 711 1026 1059 Size: 711 315 33 Fragments arranged by size: 711 315 33 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 819 854 1059 Size: 819 35 205 Fragments arranged by size: 819 205 35 DraIII CAC_nnn'GTG Cuts at: 0 640 1059 Size: 640 419 EarI CTCTTCn'nnn_ Cuts at: 0 600 789 943 1059 Size: 600 189 154 116 Fragments arranged by size: 600 189 154 116 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 365 1059 Size: 365 694 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 773 1059 Size: 773 286 EcoO109I rG'GnC_Cy Cuts at: 0 748 817 1059 Size: 748 69 242 Fragments arranged by size: 748 242 69 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 575 1059 Size: 575 484 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 427 459 1059 Size: 427 32 600 Fragments arranged by size: 600 427 32 HindIII A'AGCT_T Cuts at: 0 84 136 1059 Size: 84 52 923 Fragments arranged by size: 923 84 52 MfeI C'AATT_G Cuts at: 0 254 1059 Size: 254 805 NcoI C'CATG_G Cuts at: 0 212 233 1059 Size: 212 21 826 Fragments arranged by size: 826 212 21 NdeI CA'TA_TG Cuts at: 0 990 1059 Size: 990 69 PflMI CCAn_nnn'nTGG Cuts at: 0 994 1059 Size: 994 65 PfoI T'CCnGG_A Cuts at: 0 1011 1059 Size: 1011 48 PmlI CAC'GTG Cuts at: 0 637 1059 Size: 637 422 PpuMI rG'GwC_Cy Cuts at: 0 748 817 1059 Size: 748 69 242 Fragments arranged by size: 748 242 69 PsiI TTA'TAA Cuts at: 0 917 1059 Size: 917 142 SfcI C'TryA_G Cuts at: 0 287 1059 Size: 287 772 SmlI C'TyrA_G Cuts at: 0 81 1059 Size: 81 978 StyI C'CwwG_G Cuts at: 0 212 233 1059 Size: 212 21 826 Fragments arranged by size: 826 212 21 XbaI T'CTAG_A Cuts at: 0 565 1059 Size: 565 494 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 529 1059 Size: 529 530 XmnI GAAnn'nnTTC Cuts at: 0 20 1059 Size: 20 1039 Enzymes that do cut: AflII ApoI BamHI BanII BbvCI BmgBI BpmI Bpu10I BsaAI BseRI Bsp1286I BstYI BtgI BtsI CspCI DraIII EarI EciI Eco57MI EcoO109I FalI Hin4I HindIII MfeI NcoI NdeI PflMI PfoI PmlI PpuMI PsiI SfcI SmlI StyI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI DrdI EaeI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PpiI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XhoI XmaI ZraI