################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccIII AclI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbrPI BbvCI BciVI BclI BglI BlnI BsaI BseAI BsiWI BsmBI BsmI Bsp1407I BspEI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CpoI DraI Eam1105I Eco47III EcoO65I EcoRV FbaI FseI HpaI KpnI MfeI MluI MroI MunI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 82 GTAGAC GT^[AC][GT]AC AflII 1858 CTTAAG C^TTAAG AflIII 1476 ACACGT A^C[GA][CT]GT AgeI 1098 ACCGGT A^CCGGT Alw44I 337 GTGCAC G^TGCAC AocI 2042 CCTCAGG CC^T[ACGT]AGG ApaLI 337 GTGCAC G^TGCAC AspHI 337 GTGCAC G[AT]GC[AT]^C AviII 1773 TGCGCA TGC^GCA BalI 1190 TGGCCA TGG^CCA BanII 73 GGGCTC G[GA]GC[CT]^C BcgI 1776 GCAGAACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1858 CTTAAG C^TTAAG BsaAI 1477 CACGTA [CT]AC^GT[GA] BsaBI 934 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 26 CGGCCG CG[GA][CT]^CG BsiHKAI 337 GTGCAC G[AT]GC[AT]^C BspDI 1216 ATCGAT AT^CGAT BspMI 98 ACCTGC ACCTGC(4/8) BsrBI 473 GAGCGG CCGCTC(-3/-3) BstXI 2027 CCAAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2042 CCTCAGG CC^T[ACGT]AGG ClaI 1216 ATCGAT AT^CGAT EagI 26 CGGCCG C^GGCCG EclXI 26 CGGCCG C^GGCCG Eco52I 26 CGGCCG C^GGCCG Eco81I 2042 CCTCAGG CC^T[ACGT]AGG EcoRI 885 GAATTC G^AATTC EcoT22I 326 ATGCAT ATGCA^T FspI 1773 TGCGCA TGC^GCA HincII 1102 GTTGAC GT[CT]^[GA]AC HindII 1102 GTTGAC GT[CT]^[GA]AC KspI 521 CCGCGG CCGC^GG MaeII 1478 ACGT A^CGT MamI 934 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1190 TGGCCA TGG^CCA MscI 1190 TGGCCA TGG^CCA NcoI 1455 CCATGG C^CATGG NdeI 1111 CATATG CA^TATG NsiI 326 ATGCAT ATGCA^T PinAI 1098 ACCGGT A^CCGGT PvuII 268 CAGCTG CAG^CTG SacII 521 CCGCGG CCGC^GG SphI 1667 GCATGC GCATG^C TaqI 1217 TCGA T^CGA TthHB8I 1217 TCGA T^CGA XbaI 739 TCTAGA T^CTAGA XcmI 2027 CCAAAGAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AlwNI 1244 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG 1906 CAGAAACTG Asp700I 1018 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1534 GAACCTTTTC BbeI 385 GGCGCC GGCGC^C 418 GGCGCC BglII 90 AGATCT A^GATCT 1461 AGATCT BlpI 60 GCTGAGC GC^T[ACGT]AGC 174 GCTCAGC BmyI 73 GGGCTC G[AGT]GC[ACT]^C 337 GTGCAC BpmI 1990 CTCCAG CTGGAG(16/14) 829 CTGGAG Bpu1102I 60 GCTGAGC GC^T[ACGT]AGC 174 GCTCAGC BsaWI 406 ACCGGA [AT]^CCGG[AT] 1098 ACCGGT BsmAI 750 GAGAC GTCTC(1/5) 1726 GTCTC Bsp1286I 73 GGGCTC G[AGT]GC[ACT]^C 337 GTGCAC CelII 60 GCTGAGC GC^T[ACGT]AGC 174 GCTCAGC DraII 296 GGGTCCC [GA]G^G[ACGT]CC[CT] 464 GGGACCC DraIII 340 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG 1315 CACTCTGTG DrdI 1002 GACTTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1717 GACCTACAAGTC EcoNI 120 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 856 CCTCTTCCAGG EcoO109I 296 GGGTCCC [GA]G^G[ACGT]CC[CT] 464 GGGACCC HaeII 385 GGCGCC [GA]GCGC^[CT] 418 GGCGCC HgaI 496 GACGC GACGC(5/10) 547 GACGC HindIII 1420 AAGCTT A^AGCTT 1628 AAGCTT HphI 50 TCACC GGTGA(8/7) 1096 TCACC KasI 385 GGCGCC G^GCGCC 418 GGCGCC MspA1I 268 CAGCTG C[AC]G^C[GT]G 521 CCGCGG NaeI 28 GCCGGC GCC^GGC 415 GCCGGC NarI 385 GGCGCC GG^CGCC 418 GGCGCC NgoMIV 28 GCCGGC G^CCGGC 415 GCCGGC NspI 1667 GCATGC [GA]CATG^[CT] 1691 GCATGT PpuMI 296 GGGTCCC [GA]G^G[AT]CC[CT] 464 GGGACCC PstI 124 CTGCAG CTGCA^G 1027 CTGCAG SfaNI 325 GATGC GCATC(5/9) 836 GCATC SfoI 385 GGCGCC GGC^GCC 418 GGCGCC SmlI 1602 CTTGAG C^T[CT][GA]AG 1858 CTTAAG XmnI 1018 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1534 GAACCTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AvaI AvaII BanI BbsI BbvI BcnI BfaI BpuAI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmFI BsoBI BspHI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RcaI RsaI Sau3AI Sau96I ScrFI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII