(Circular) (Six-base) MAPSORT of: pFN21AB6269.seq Check: 8891 from: 1 to: 6787 pFN21AB6269 6787 bp With 182 enzymes: SgfI * March 28, 2012 12:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4597 278 Size: 53 83 186 936 3061 2468 Fragments arranged by size: 3061 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2088 4130 1937 Size: 151 2042 4594 Fragments arranged by size: 4594 2042 151 Acc65I G'GTAC_C Cuts at: 4108 4108 Size: 6787 AclI AA'CG_TT Cuts at: 1563 4914 5287 1563 Size: 3351 373 3063 Fragments arranged by size: 3351 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2036 2657 2880 2902 4850 6234 6502 1632 Size: 404 621 223 22 1948 1384 268 1917 Fragments arranged by size: 1948 1917 1384 621 404 268 223 22 AflII C'TTAA_G Cuts at: 820 1017 3863 820 Size: 197 2846 3744 Fragments arranged by size: 3744 2846 197 AflIII A'CryG_T Cuts at: 1203 3481 5686 6663 1203 Size: 2278 2205 977 1327 Fragments arranged by size: 2278 2205 1327 977 AgeI A'CCGG_T Cuts at: 3103 5618 3103 Size: 2515 4272 AhdI GACnn_n'nnGTC Cuts at: 5516 5516 Size: 6787 AleI CACnn'nnGTG Cuts at: 1166 2324 1166 Size: 1158 5629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6787 AlwNI CAG_nnn'CTG Cuts at: 3254 3916 4530 6102 3254 Size: 662 614 1572 3939 Fragments arranged by size: 3939 1572 662 614 ApaLI G'TGCA_C Cuts at: 2342 4844 6000 2342 Size: 2502 1156 3129 Fragments arranged by size: 3129 2502 1156 ApoI r'AATT_y Cuts at: 1493 2827 2890 3040 3817 4096 4322 4474 6583 Size: 1334 63 150 777 279 226 152 2109 Cuts at: 6583 1493 Size: 1697 Fragments arranged by size: 2109 1697 1334 777 279 226 152 150 63 AseI AT'TA_AT Cuts at: 160 1032 5341 160 Size: 872 4309 1606 Fragments arranged by size: 4309 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6787 AvaI C'yCGr_G Cuts at: 1870 1943 2197 2264 2297 2475 4112 1870 Size: 73 254 67 33 178 1637 4545 Fragments arranged by size: 4545 1637 254 178 73 67 33 BamHI G'GATC_C Cuts at: 4117 4484 4117 Size: 367 6420 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2390 2423 2521 2545 4108 Size: 325 217 627 603 33 98 24 1563 Cuts at: 4108 5563 618 Size: 1455 1842 Fragments arranged by size: 1842 1563 1455 627 603 325 217 98 33 24 BanII G_rGCy'C Cuts at: 1391 2082 4106 1391 Size: 691 2024 4072 Fragments arranged by size: 4072 2024 691 BbeI G_GCGC'C Cuts at: 2394 2427 2394 Size: 33 6754 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2799 3210 3597 928 Size: 971 900 411 387 4118 Fragments arranged by size: 4118 971 900 411 387 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3770 3804 4978 5012 1903 Size: 34 1833 34 1174 34 3678 Fragments arranged by size: 3678 1833 1174 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4679 5889 4679 Size: 1210 5577 BclI T'GATC_A Cuts at: 1548 1548 Size: 6787 BfrBI ATG'CAT Cuts at: 2333 2333 Size: 6787 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5398 136 Size: 107 122 71 631 605 3726 1525 Fragments arranged by size: 3726 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2095 3466 6782 2095 Size: 1371 3316 2100 Fragments arranged by size: 3316 2100 1371 BlpI GC'TnA_GC Cuts at: 2066 2180 4204 2066 Size: 114 2024 4649 Fragments arranged by size: 4649 2024 114 Bme1580I G_kGCm'C Cuts at: 1790 2346 4848 6004 1790 Size: 556 2502 1156 2573 Fragments arranged by size: 2573 2502 1156 556 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6787 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2728 3414 4518 Size: 416 53 83 399 24 1311 686 1104 Cuts at: 4518 5476 442 Size: 958 1753 Fragments arranged by size: 1753 1311 1104 958 686 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6787 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2860 2860 Size: 6787 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2855 3980 5447 1239 Size: 1616 1125 1467 2579 Fragments arranged by size: 2579 1616 1467 1125 Bpu10I CC'TnA_GC Cuts at: 2541 3060 2541 Size: 519 6268 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3628 4912 5777 6075 3628 Size: 1284 865 298 4340 Fragments arranged by size: 4340 1284 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5450 882 Size: 617 3951 2219 Fragments arranged by size: 3951 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3484 4578 493 Size: 711 2280 1094 2702 Fragments arranged by size: 2702 2280 1094 711 BsaBI GATnn'nnATC Cuts at: 2943 6781 2943 Size: 3838 2949 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2391 2424 2502 4594 Size: 53 83 186 936 858 33 78 2092 Cuts at: 4594 4976 5611 275 Size: 382 635 1451 Fragments arranged by size: 2092 1451 936 858 635 382 186 83 78 53 33 BsaWI w'CCGG_w Cuts at: 2411 3103 5220 5618 5892 6039 6450 2411 Size: 692 2117 398 274 147 411 2748 Fragments arranged by size: 2748 2117 692 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6562 Fragments arranged by size: 6562 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2430 2528 2588 1186 Size: 1244 98 60 5385 Fragments arranged by size: 5385 1244 98 60 BseYI C'CCAG_C Cuts at: 2622 5990 6486 2622 Size: 3368 496 2923 Fragments arranged by size: 3368 2923 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2033 2474 3504 1163 Size: 573 297 441 1030 4446 Fragments arranged by size: 4446 1030 573 441 297 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 4237 4998 6026 1540 Size: 275 186 33 2203 761 1028 2301 Fragments arranged by size: 2301 2203 1028 761 275 186 33 BsiHKAI G_wGCw'C Cuts at: 2346 4848 4933 6004 2346 Size: 2502 85 1071 3129 Fragments arranged by size: 3129 2502 1071 85 BsmI GAATG_Cn' Cuts at: 4298 4391 4298 Size: 93 6694 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2346 4106 4848 4933 6004 1391 Size: 399 292 264 1760 742 85 1071 2174 Fragments arranged by size: 2174 1760 1071 742 399 292 264 85 BspEI T'CCGG_A Cuts at: 6450 6450 Size: 6787 BspHI T'CATG_A Cuts at: 2950 3379 3931 4676 6406 2950 Size: 429 552 745 1730 3331 Fragments arranged by size: 3331 1730 745 552 429 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2112 4142 844 Size: 1268 2030 3489 Fragments arranged by size: 3489 2030 1268 BsrBI CCG'CTC Cuts at: 1394 1823 2480 4674 1394 Size: 429 657 2194 3507 Fragments arranged by size: 3507 2194 657 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5282 5456 65 Size: 1183 4034 174 1396 Fragments arranged by size: 4034 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2033 2420 3103 5431 5618 1700 Size: 110 223 387 683 2328 187 2869 Fragments arranged by size: 2869 2328 683 387 223 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6787 BssHII G'CGCG_C Cuts at: 1925 2212 2290 2292 1925 Size: 287 78 2 6420 Fragments arranged by size: 6420 287 78 2 BssSI C'ACGA_G Cuts at: 4847 5859 4847 Size: 1012 5775 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6787 BstBI TT'CG_AA Cuts at: 5591 5591 Size: 6787 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6787 BstXI CCAn_nnnn'nTGG Cuts at: 4039 4039 Size: 6787 BstYI r'GATC_y Cuts at: 1972 2095 2992 3466 4117 4484 4869 4886 6338 Size: 123 897 474 651 367 385 17 1452 Cuts at: 6338 6424 6436 6782 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 897 651 474 385 367 346 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4048 4048 Size: 6787 BtgI C'CryG_G Cuts at: 513 1065 2377 2526 3460 3548 513 Size: 552 1312 149 934 88 3752 Fragments arranged by size: 3752 1312 934 552 149 88 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6048 Fragments arranged by size: 6048 683 56 BtsI GCAGTG_nn' Cuts at: 4303 5097 5117 4303 Size: 794 20 5973 Fragments arranged by size: 5973 794 20 ClaI AT'CG_AT Cuts at: 1554 3222 1554 Size: 1668 5119 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6752 DraI TTT'AAA Cuts at: 4091 4091 Size: 6787 DraIII CAC_nnn'GTG Cuts at: 2350 3325 2350 Size: 975 5812 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3013 3728 5794 809 Size: 507 1697 715 2066 1802 Fragments arranged by size: 2066 1802 1697 715 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2160 3195 Size: 54 1425 50 275 18 201 129 1035 Cuts at: 3195 4234 5123 6752 8 Size: 1039 889 1629 43 Fragments arranged by size: 1629 1425 1039 1035 889 275 201 129 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2031 4234 1537 Size: 275 219 2203 4090 Fragments arranged by size: 4090 2203 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 2505 2868 2983 3751 4717 1353 Size: 1152 363 115 768 966 3423 Fragments arranged by size: 3423 1152 966 768 363 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2158 2574 5374 5748 5894 387 Size: 1064 138 569 416 2800 374 146 1280 Fragments arranged by size: 2800 1280 1064 569 416 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2657 2855 2880 2902 3980 4850 Size: 393 404 621 198 25 22 1078 870 Cuts at: 4850 5447 6234 6502 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1078 870 787 621 597 404 393 268 198 25 22 EcoNI CCTnn'n_nnAGG Cuts at: 2129 2865 2129 Size: 736 6051 EcoO109I rG'GnC_Cy Cuts at: 2302 2470 2302 Size: 168 6619 EcoRI G'AATT_C Cuts at: 2890 4096 4474 2890 Size: 1206 378 5203 Fragments arranged by size: 5203 1206 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3752 3752 Size: 6787 FspI TGC'GCA Cuts at: 3780 5293 3780 Size: 1513 5274 HaeII r_GCGC'y Cuts at: 1678 2394 2427 5934 1678 Size: 716 33 3507 2531 Fragments arranged by size: 3507 2531 716 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3350 3382 5428 5460 5502 Size: 32 135 32 1778 32 2046 32 42 Cuts at: 5502 5534 1373 Size: 32 2626 Fragments arranged by size: 2626 2046 1778 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3109 4131 4377 669 Size: 988 281 1171 1022 246 3079 Fragments arranged by size: 3079 1171 1022 988 281 246 HindIII A'AGCT_T Cuts at: 748 3425 3633 748 Size: 2677 208 3902 Fragments arranged by size: 3902 2677 208 HpaI GTT'AAC Cuts at: 4377 4377 Size: 6787 KasI G'GCGC_C Cuts at: 2390 2423 2390 Size: 33 6754 KpnI G_GTAC'C Cuts at: 4112 4112 Size: 6787 MfeI C'AATT_G Cuts at: 4386 4386 Size: 6787 MluI A'CGCG_T Cuts at: 6663 6663 Size: 6787 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3020 5901 6085 1305 Size: 1715 2881 184 2007 Fragments arranged by size: 2881 2007 1715 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3197 10 Size: 54 1425 343 1365 3600 Fragments arranged by size: 3600 1425 1365 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2324 2763 2907 4745 5104 5263 518 Size: 648 1158 439 144 1838 359 159 2042 Fragments arranged by size: 2042 1838 1158 648 439 359 159 144 MspA1I CmG'CkG Cuts at: 1606 2275 2528 4203 4880 6028 6273 1606 Size: 669 253 1675 677 1148 245 2120 Fragments arranged by size: 2120 1675 1148 677 669 253 245 NaeI GCC'GGC Cuts at: 2035 2422 2035 Size: 387 6400 NarI GG'CG_CC Cuts at: 2391 2424 2391 Size: 33 6754 NcoI C'CATG_G Cuts at: 513 1065 3460 513 Size: 552 2395 3840 Fragments arranged by size: 3840 2395 552 NdeI CA'TA_TG Cuts at: 387 3117 387 Size: 2730 4057 NgoMIV G'CCGG_C Cuts at: 2033 2420 2033 Size: 387 6400 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6787 NotI GC'GGCC_GC Cuts at: 4234 4234 Size: 6787 NsiI A_TGCA'T Cuts at: 2335 2335 Size: 6787 NspI r_CATG'y Cuts at: 1132 1229 3676 3700 4145 5690 1132 Size: 97 2447 24 445 1545 2229 Fragments arranged by size: 2447 2229 1545 445 97 24 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6787 PciI A'CATG_T Cuts at: 5686 5686 Size: 6787 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6787 PfoI T'CCnGG_A Cuts at: 2280 3073 2280 Size: 793 5994 PmeI GTTT'AAAC Cuts at: 4091 4091 Size: 6787 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2129 2161 4856 4888 6369 6401 2129 Size: 32 2695 32 1481 32 2515 Fragments arranged by size: 2695 2515 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2302 2470 2302 Size: 168 6619 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6787 PsiI TTA'TAA Cuts at: 4357 4357 Size: 6787 PstI C_TGCA'G Cuts at: 830 2133 3036 4139 830 Size: 1303 903 1103 3478 Fragments arranged by size: 3478 1303 1103 903 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6787 PvuII CAG'CTG Cuts at: 2275 2275 Size: 6787 SacII CC_GC'GG Cuts at: 2529 2529 Size: 6787 SalI G'TCGA_C Cuts at: 1936 4129 1936 Size: 2193 4594 SanDI GG'GwC_CC Cuts at: 2302 2470 2302 Size: 168 6619 SbfI CC_TGCA'GG Cuts at: 4139 4139 Size: 6787 ScaI AGT'ACT Cuts at: 5035 6747 5035 Size: 1712 5075 SfcI C'TryA_G Cuts at: 826 1045 2129 3032 3342 3396 3654 4135 5270 Size: 219 1084 903 310 54 258 481 1135 Cuts at: 5270 5951 6142 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1084 903 681 481 310 258 219 191 54 SfoI GGC'GCC Cuts at: 2392 2425 2392 Size: 33 6754 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6787 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6787 SmaI CCC'GGG Cuts at: 1872 4114 1872 Size: 2242 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3607 3863 4891 5792 6054 820 Size: 197 926 1664 256 1028 901 262 1553 Fragments arranged by size: 1664 1553 1028 926 901 262 256 197 SnaBI TAC'GTA Cuts at: 493 4578 493 Size: 4085 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6787 SphI G_CATG'C Cuts at: 1132 3676 4145 1132 Size: 2544 469 3774 Fragments arranged by size: 3774 2544 469 SspI AAT'ATT Cuts at: 5 52 4711 5 Size: 47 4659 2081 Fragments arranged by size: 4659 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6787 StyI C'CwwG_G Cuts at: 513 1065 2232 2496 3460 3961 4226 513 Size: 552 1167 264 964 501 265 3074 Fragments arranged by size: 3074 1167 964 552 501 265 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4814 4831 4984 5169 5614 325 Size: 1255 3234 17 153 185 445 1498 Fragments arranged by size: 3234 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5033 6745 96 Size: 274 80 33 51 1443 3056 1712 138 Fragments arranged by size: 3056 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6747 6779 6747 Size: 32 6755 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6787 XbaI T'CTAG_A Cuts at: 2744 4123 2744 Size: 1379 5408 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4039 4039 Size: 6787 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6787 XmaI C'CCGG_G Cuts at: 1870 4112 1870 Size: 2242 4545 XmnI GAAnn'nnTTC Cuts at: 3027 3543 4916 3027 Size: 516 1373 4898 Fragments arranged by size: 4898 1373 516 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4595 276 Size: 53 83 186 936 3061 2468 Fragments arranged by size: 3061 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI