(Linear) (Six-base) MAPSORT of: KIBB6269.seq Check: 3380 from: 1 to: 2082 KIBB6269.orf With 182 enzymes: SgfI * March 22, 2012 15:34 .. AccI GT'mk_AC Cuts at: 0 83 2082 Size: 83 1999 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 652 875 897 2082 Size: 31 621 223 22 1185 Fragments arranged by size: 1185 621 223 31 22 AflII C'TTAA_G Cuts at: 0 1858 2082 Size: 1858 224 AflIII A'CryG_T Cuts at: 0 1476 2082 Size: 1476 606 AgeI A'CCGG_T Cuts at: 0 1098 2082 Size: 1098 984 AleI CACnn'nnGTG Cuts at: 0 319 2082 Size: 319 1763 AlwNI CAG_nnn'CTG Cuts at: 0 1249 1911 2082 Size: 1249 662 171 Fragments arranged by size: 1249 662 171 ApaLI G'TGCA_C Cuts at: 0 337 2082 Size: 337 1745 ApoI r'AATT_y Cuts at: 0 822 885 1035 1812 2082 Size: 822 63 150 777 270 Fragments arranged by size: 822 777 270 150 63 AvaI C'yCGr_G Cuts at: 0 192 259 292 470 2082 Size: 192 67 33 178 1612 Fragments arranged by size: 1612 192 178 67 33 BanI G'GyrC_C Cuts at: 0 385 418 516 540 2082 Size: 385 33 98 24 1542 Fragments arranged by size: 1542 385 98 33 24 BanII G_rGCy'C Cuts at: 0 77 2082 Size: 77 2005 BbeI G_GCGC'C Cuts at: 0 389 422 2082 Size: 389 33 1660 Fragments arranged by size: 1660 389 33 BbsI GAAGACnn'nnnn_ Cuts at: 0 794 1205 1592 2082 Size: 794 411 387 490 Fragments arranged by size: 794 490 411 387 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1765 1799 2082 Size: 1765 34 283 Fragments arranged by size: 1765 283 34 BfrBI ATG'CAT Cuts at: 0 328 2082 Size: 328 1754 BglII A'GATC_T Cuts at: 0 90 1461 2082 Size: 90 1371 621 Fragments arranged by size: 1371 621 90 BlpI GC'TnA_GC Cuts at: 0 61 175 2082 Size: 61 114 1907 Fragments arranged by size: 1907 114 61 Bme1580I G_kGCm'C Cuts at: 0 341 2082 Size: 341 1741 BmrI ACTGGGnnnn_n' Cuts at: 0 723 1409 2082 Size: 723 686 673 Fragments arranged by size: 723 686 673 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 855 2082 Size: 855 1227 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 850 1975 2082 Size: 850 1125 107 Fragments arranged by size: 1125 850 107 Bpu10I CC'TnA_GC Cuts at: 0 536 1055 2082 Size: 536 519 1027 Fragments arranged by size: 1027 536 519 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1623 2082 Size: 1623 459 BsaAI yAC'GTr Cuts at: 0 1479 2082 Size: 1479 603 BsaBI GATnn'nnATC Cuts at: 0 938 2082 Size: 938 1144 BsaHI Gr'CG_yC Cuts at: 0 386 419 497 2082 Size: 386 33 78 1585 Fragments arranged by size: 1585 386 78 33 BsaWI w'CCGG_w Cuts at: 0 406 1098 2082 Size: 406 692 984 Fragments arranged by size: 984 692 406 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 425 523 583 2082 Size: 425 98 60 1499 Fragments arranged by size: 1499 425 98 60 BseYI C'CCAG_C Cuts at: 0 617 2082 Size: 617 1465 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 469 1499 2082 Size: 28 441 1030 583 Fragments arranged by size: 1030 583 441 28 BsiEI CG_ry'CG Cuts at: 0 29 2082 Size: 29 2053 BsiHKAI G_wGCw'C Cuts at: 0 341 2082 Size: 341 1741 Bsp1286I G_dGCh'C Cuts at: 0 77 341 2082 Size: 77 264 1741 Fragments arranged by size: 1741 264 77 BspHI T'CATG_A Cuts at: 0 945 1374 1926 2082 Size: 945 429 552 156 Fragments arranged by size: 945 552 429 156 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 107 2082 Size: 107 1975 BsrBI CCG'CTC Cuts at: 0 475 2082 Size: 475 1607 BsrFI r'CCGG_y Cuts at: 0 28 415 1098 2082 Size: 28 387 683 984 Fragments arranged by size: 984 683 387 28 BssHII G'CGCG_C Cuts at: 0 207 285 287 2082 Size: 207 78 2 1795 Fragments arranged by size: 1795 207 78 2 BstXI CCAn_nnnn'nTGG Cuts at: 0 2034 2082 Size: 2034 48 BstYI r'GATC_y Cuts at: 0 90 987 1461 2082 Size: 90 897 474 621 Fragments arranged by size: 897 621 474 90 Bsu36I CC'TnA_GG Cuts at: 0 2043 2082 Size: 2043 39 BtgI C'CryG_G Cuts at: 0 372 521 1455 1543 2082 Size: 372 149 934 88 539 Fragments arranged by size: 934 539 372 149 88 ClaI AT'CG_AT Cuts at: 0 1217 2082 Size: 1217 865 DraIII CAC_nnn'GTG Cuts at: 0 345 1320 2082 Size: 345 975 762 Fragments arranged by size: 975 762 345 DrdI GACnn_nn'nnGTC Cuts at: 0 1008 1723 2082 Size: 1008 715 359 Fragments arranged by size: 1008 715 359 EaeI y'GGCC_r Cuts at: 0 26 155 1190 2082 Size: 26 129 1035 892 Fragments arranged by size: 1035 892 129 26 EagI C'GGCC_G Cuts at: 0 26 2082 Size: 26 2056 EarI CTCTTCn'nnn_ Cuts at: 0 500 863 978 1746 2082 Size: 500 363 115 768 336 Fragments arranged by size: 768 500 363 336 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 153 569 2082 Size: 153 416 1513 Fragments arranged by size: 1513 416 153 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 652 850 875 897 1975 2082 Size: 31 621 198 25 22 1078 107 Fragments arranged by size: 1078 621 198 107 31 25 22 EcoNI CCTnn'n_nnAGG Cuts at: 0 124 860 2082 Size: 124 736 1222 Fragments arranged by size: 1222 736 124 EcoO109I rG'GnC_Cy Cuts at: 0 297 465 2082 Size: 297 168 1617 Fragments arranged by size: 1617 297 168 EcoRI G'AATT_C Cuts at: 0 885 2082 Size: 885 1197 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1747 2082 Size: 1747 335 FspI TGC'GCA Cuts at: 0 1775 2082 Size: 1775 307 HaeII r_GCGC'y Cuts at: 0 389 422 2082 Size: 389 33 1660 Fragments arranged by size: 1660 389 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1345 1377 2082 Size: 1345 32 705 Fragments arranged by size: 1345 705 32 HincII GTy'rAC Cuts at: 0 1104 2082 Size: 1104 978 HindIII A'AGCT_T Cuts at: 0 1420 1628 2082 Size: 1420 208 454 Fragments arranged by size: 1420 454 208 KasI G'GCGC_C Cuts at: 0 385 418 2082 Size: 385 33 1664 Fragments arranged by size: 1664 385 33 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1015 2082 Size: 1015 1067 MscI TGG'CCA Cuts at: 0 1192 2082 Size: 1192 890 MslI CAynn'nnrTG Cuts at: 0 319 758 902 2082 Size: 319 439 144 1180 Fragments arranged by size: 1180 439 319 144 MspA1I CmG'CkG Cuts at: 0 270 523 2082 Size: 270 253 1559 Fragments arranged by size: 1559 270 253 NaeI GCC'GGC Cuts at: 0 30 417 2082 Size: 30 387 1665 Fragments arranged by size: 1665 387 30 NarI GG'CG_CC Cuts at: 0 386 419 2082 Size: 386 33 1663 Fragments arranged by size: 1663 386 33 NcoI C'CATG_G Cuts at: 0 1455 2082 Size: 1455 627 NdeI CA'TA_TG Cuts at: 0 1112 2082 Size: 1112 970 NgoMIV G'CCGG_C Cuts at: 0 28 415 2082 Size: 28 387 1667 Fragments arranged by size: 1667 387 28 NsiI A_TGCA'T Cuts at: 0 330 2082 Size: 330 1752 NspI r_CATG'y Cuts at: 0 1671 1695 2082 Size: 1671 24 387 Fragments arranged by size: 1671 387 24 PfoI T'CCnGG_A Cuts at: 0 275 1068 2082 Size: 275 793 1014 Fragments arranged by size: 1014 793 275 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 124 156 2082 Size: 124 32 1926 Fragments arranged by size: 1926 124 32 PpuMI rG'GwC_Cy Cuts at: 0 297 465 2082 Size: 297 168 1617 Fragments arranged by size: 1617 297 168 PstI C_TGCA'G Cuts at: 0 128 1031 2082 Size: 128 903 1051 Fragments arranged by size: 1051 903 128 PvuII CAG'CTG Cuts at: 0 270 2082 Size: 270 1812 SacII CC_GC'GG Cuts at: 0 524 2082 Size: 524 1558 SanDI GG'GwC_CC Cuts at: 0 297 465 2082 Size: 297 168 1617 Fragments arranged by size: 1617 297 168 SfcI C'TryA_G Cuts at: 0 124 1027 1337 1391 1649 2082 Size: 124 903 310 54 258 433 Fragments arranged by size: 903 433 310 258 124 54 SfoI GGC'GCC Cuts at: 0 387 420 2082 Size: 387 33 1662 Fragments arranged by size: 1662 387 33 SmlI C'TyrA_G Cuts at: 0 1602 1858 2082 Size: 1602 256 224 Fragments arranged by size: 1602 256 224 SphI G_CATG'C Cuts at: 0 1671 2082 Size: 1671 411 StyI C'CwwG_G Cuts at: 0 227 491 1455 1956 2082 Size: 227 264 964 501 126 Fragments arranged by size: 964 501 264 227 126 XbaI T'CTAG_A Cuts at: 0 739 2082 Size: 739 1343 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2034 2082 Size: 2034 48 XmnI GAAnn'nnTTC Cuts at: 0 1022 1538 2082 Size: 1022 516 544 Fragments arranged by size: 1022 544 516 Enzymes that do cut: AccI AcuI AflII AflIII AgeI AleI AlwNI ApaLI ApoI AvaI BanI BanII BbeI BbsI BcgI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BstXI BstYI Bsu36I BtgI ClaI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PfoI PpiI PpuMI PstI PvuII SacII SanDI SfcI SfoI SmlI SphI StyI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BglI BmgBI BmtI BsaI BsaXI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI CspCI DraI EcoICRI EcoRV FseI FspAI HpaI KpnI MfeI MluI NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XhoI XmaI ZraI