Restriction map for
pFN21AE3345
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AvaI BalI
BanI BbeI BbrPI BbvCI BcgI
BciVI BcnI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI BspEI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I CpoI DraII DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoT22I FauI FseI HaeII HapII
Hin1I HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I StuI Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1138 GTCGAC GT^[AC][GT]AC
AciI 32 CCGC CCGC(-3/-1)
AflIII 1346 ACATGT A^C[GA][CT]GT
ApaI 143 GGGCCC GGGCC^C
Asp700I 2091 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1671 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2021 TGCGCA TGC^GCA
AvrII 2437 CCTAGG C^CTAGG
BamHI 678 GGATCC G^GATCC
BglI 762 GCCCAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2184 AGATCT A^GATCT
BlnI 2437 CCTAGG C^CTAGG
BlpI 2197 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2197 GCTGAGC GC^T[ACGT]AGC
BsaI 2158 GGTCTC GGTCTC(1/5)
BsgI 1558 CTGCAC GTGCAG(16/14)
BsmI 2451 GAATGC GAATGC(1/-1)
Bsp1407I 1343 TGTACA T^GTACA
BspDI 368 ATCGAT AT^CGAT
BspLU11I 1346 ACATGT A^CATGT
BspMI 2709 ACCTGC ACCTGC(4/8)
BsrGI 1343 TGTACA T^GTACA
BstAPI 2526 GCATACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2197 GCTGAGC GC^T[ACGT]AGC
CfoI 2022 GCGC GCG^C
ClaI 368 ATCGAT AT^CGAT
DrdI 1141 GACATCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 169 GATATC GAT^ATC
FspI 2021 TGCGCA TGC^GCA
HhaI 2022 GCGC GCG^C
HinP1I 2022 GCGC G^CGC
HindIII 1911 AAGCTT A^AGCTT
PflFI 1671 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 1138 GTCGAC G^TCGAC
SapI 955 GCTCTTC GCTCTTC(1/4)
SpeI 2077 ACTAGT A^CTAGT
SspBI 1343 TGTACA T^GTACA
SwaI 1856 ATTTAAAT ATTT^AAAT
Tth111I 1671 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 22 TCTAGA T^CTAGA
XcmI 215 CCAGTTCAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2091 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AflII 610 CTTAAG C^TTAAG
774 CTTAAG
BanII 143 GGGCCC G[GA]GC[CT]^C
2200 GAGCCC
BfrI 610 CTTAAG C^TTAAG
774 CTTAAG
BseRI 2848 CTCCTC GAGGAG(10/8)
1519 GAGGAG
BstYI 678 GGATCC [GA]^GATC[CT]
2184 AGATCT
EcoNI 752 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2122 CCTGAAATAGG
MaeII 881 ACGT A^CGT
2238 ACGT
MflI 678 GGATCC [GA]^GATC[CT]
2184 AGATCT
MspA1I 1028 CAGCTG C[AC]G^C[GT]G
2017 CAGCTG
PstI 400 CTGCAG CTGCA^G
1012 CTGCAG
PvuII 1028 CAGCTG CAG^CTG
2017 CAGCTG
ScaI 297 AGTACT AGT^ACT
1761 AGTACT
TaqI 369 TCGA T^CGA
1139 TCGA
TthHB8I 369 TCGA T^CGA
1139 TCGA
XhoII 678 GGATCC [GA]^GATC[CT]
2184 AGATCT
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BbsI BbvI BclI
BfaI BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HgaI HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI