(Circular) (Six-base) MAPSORT of: pFN21AE3345.seq Check: 7693 from: 1 to: 7762 pFN21AE3345 7762 bp With 182 enzymes: SgfI * March 28, 2012 12:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5572 278 Size: 53 83 186 936 4036 2468 Fragments arranged by size: 4036 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3144 5105 1937 Size: 1207 1961 4594 Fragments arranged by size: 4594 1961 1207 Acc65I G'GTAC_C Cuts at: 5083 5083 Size: 7762 AclI AA'CG_TT Cuts at: 1563 5889 6262 1563 Size: 4326 373 3063 Fragments arranged by size: 4326 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2223 2295 2574 2743 3092 4377 5825 7209 Size: 591 72 279 169 349 1285 1448 1384 Cuts at: 7209 7477 1632 Size: 268 1917 Fragments arranged by size: 1917 1448 1384 1285 591 349 279 268 169 72 AflII C'TTAA_G Cuts at: 820 1017 2615 2779 820 Size: 197 1598 164 5803 Fragments arranged by size: 5803 1598 197 164 AflIII A'CryG_T Cuts at: 1203 3351 6661 7638 1203 Size: 2148 3310 977 1327 Fragments arranged by size: 3310 2148 1327 977 AgeI A'CCGG_T Cuts at: 6593 6593 Size: 7762 AhdI GACnn_n'nnGTC Cuts at: 6491 6491 Size: 7762 AleI CACnn'nnGTG Cuts at: 1166 2781 1166 Size: 1615 6147 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2653 1184 Size: 1469 6293 AlwNI CAG_nnn'CTG Cuts at: 2920 3016 3038 3346 4528 5505 7077 2920 Size: 96 22 308 1182 977 1572 3605 Fragments arranged by size: 3605 1572 1182 977 308 96 22 ApaI G_GGCC'C Cuts at: 2152 2152 Size: 7762 ApaLI G'TGCA_C Cuts at: 5819 6975 5819 Size: 1156 6606 ApoI r'AATT_y Cuts at: 1493 2364 2391 3472 3494 4047 4483 5071 5297 Size: 871 27 1081 22 553 436 588 226 Cuts at: 5297 5449 7558 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1081 871 588 553 436 226 152 27 22 AseI AT'TA_AT Cuts at: 160 1032 6316 160 Size: 872 5284 1606 Fragments arranged by size: 5284 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7762 AvaI C'yCGr_G Cuts at: 1870 1943 5087 1870 Size: 73 3144 4545 Fragments arranged by size: 4545 3144 73 AvrII C'CTAG_G Cuts at: 4442 4442 Size: 7762 BamHI G'GATC_C Cuts at: 2683 5092 5459 2683 Size: 2409 367 4986 Fragments arranged by size: 4986 2409 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5083 6538 618 Size: 325 217 627 3296 1455 1842 Fragments arranged by size: 3296 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2152 4209 5081 1391 Size: 761 2057 872 4072 Fragments arranged by size: 4072 2057 872 761 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3080 3645 3680 3911 5036 928 Size: 971 1181 565 35 231 1125 3654 Fragments arranged by size: 3654 1181 1125 971 565 231 35 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5953 5987 1903 Size: 34 4016 34 3678 Fragments arranged by size: 4016 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5654 6864 5654 Size: 1210 6552 BclI T'GATC_A Cuts at: 1548 2461 2482 3718 1548 Size: 913 21 1236 5592 Fragments arranged by size: 5592 1236 913 21 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2773 6373 136 Size: 107 122 71 631 605 1101 3600 1525 Fragments arranged by size: 3600 1525 1101 631 605 122 107 71 BglII A'GATC_T Cuts at: 4189 7757 4189 Size: 3568 4194 BlpI GC'TnA_GC Cuts at: 4203 5179 4203 Size: 976 6786 Bme1580I G_kGCm'C Cuts at: 1790 2152 2492 2778 5823 6979 1790 Size: 362 340 286 3045 1156 2573 Fragments arranged by size: 3045 2573 1156 362 340 286 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7762 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2470 3870 5493 Size: 416 53 83 399 24 1053 1400 1623 Cuts at: 5493 6451 442 Size: 958 1753 Fragments arranged by size: 1753 1623 1400 1053 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7762 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3201 3722 4707 6422 1239 Size: 1962 521 985 1715 2579 Fragments arranged by size: 2579 1962 1715 985 521 Bpu10I CC'TnA_GC Cuts at: 2574 3267 4107 2574 Size: 693 840 6229 Fragments arranged by size: 6229 840 693 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4699 5887 6752 7050 4699 Size: 1188 865 298 5411 Fragments arranged by size: 5411 1188 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4169 6425 882 Size: 617 2670 2256 2219 Fragments arranged by size: 2670 2256 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5553 493 Size: 711 4349 2702 Fragments arranged by size: 4349 2702 711 BsaBI GATnn'nnATC Cuts at: 7756 7756 Size: 7762 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5569 5951 6586 275 Size: 53 83 186 936 4036 382 635 1451 Fragments arranged by size: 4036 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6195 6593 6867 7014 7425 6195 Size: 398 274 147 411 6532 Fragments arranged by size: 6532 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2393 2423 1178 Size: 30 165 30 990 30 6517 Fragments arranged by size: 6517 990 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3539 4844 1186 Size: 2353 1305 4104 Fragments arranged by size: 4104 2353 1305 BseYI C'CCAG_C Cuts at: 6965 7461 6965 Size: 496 7266 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3548 1163 Size: 573 1812 5377 Fragments arranged by size: 5377 1812 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5212 5973 7001 1540 Size: 275 186 3211 761 1028 2301 Fragments arranged by size: 3211 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5823 5908 6979 5823 Size: 85 1071 6606 Fragments arranged by size: 6606 1071 85 BsmI GAATG_Cn' Cuts at: 4462 5273 5366 4462 Size: 811 93 6858 Fragments arranged by size: 6858 811 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2492 2778 4209 5081 5823 5908 Size: 399 362 340 286 1431 872 742 85 Cuts at: 5908 6979 1391 Size: 1071 2174 Fragments arranged by size: 2174 1431 1071 872 742 399 362 340 286 85 BspEI T'CCGG_A Cuts at: 7425 7425 Size: 7762 BspHI T'CATG_A Cuts at: 2178 4054 4506 5651 7381 2178 Size: 1876 452 1145 1730 2559 Fragments arranged by size: 2559 1876 1730 1145 452 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4723 5117 844 Size: 3879 394 3489 Fragments arranged by size: 3879 3489 394 BsrBI CCG'CTC Cuts at: 1394 1823 5649 1394 Size: 429 3826 3507 Fragments arranged by size: 3826 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3413 3651 3798 4535 6257 6431 65 Size: 1183 2165 238 147 737 1722 174 1396 Fragments arranged by size: 2165 1722 1396 1183 737 238 174 147 BsrFI r'CCGG_y Cuts at: 1700 1810 6406 6593 1700 Size: 110 4596 187 2869 Fragments arranged by size: 4596 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3348 96 Size: 3252 4510 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7762 BssSI C'ACGA_G Cuts at: 5822 6834 5822 Size: 1012 6750 BstAPI GCAn_nnn'nTGC Cuts at: 794 4537 794 Size: 3743 4019 BstBI TT'CG_AA Cuts at: 6566 6566 Size: 7762 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7762 BstYI r'GATC_y Cuts at: 1972 2683 4189 5092 5459 5844 5861 7313 7399 Size: 711 1506 903 367 385 17 1452 86 Cuts at: 7399 7411 7757 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1506 1452 903 711 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7023 Fragments arranged by size: 7023 683 56 BtsI GCAGTG_nn' Cuts at: 2596 3450 5278 6072 6092 2596 Size: 854 1828 794 20 4266 Fragments arranged by size: 4266 1828 854 794 20 ClaI AT'CG_AT Cuts at: 1554 2374 1554 Size: 820 6942 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7727 DraI TTT'AAA Cuts at: 3841 3864 4482 4500 4593 4905 5066 3841 Size: 23 618 18 93 312 161 6537 Fragments arranged by size: 6537 618 312 161 93 23 18 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3152 6769 809 Size: 507 1836 3617 1802 Fragments arranged by size: 3617 1836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5209 6098 7727 Size: 54 1425 50 275 18 3379 889 1629 Cuts at: 7727 8 Size: 43 Fragments arranged by size: 3379 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5209 1537 Size: 275 3397 4090 Fragments arranged by size: 4090 3397 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2029 2601 2967 3960 5692 1353 Size: 676 572 366 993 1732 3423 Fragments arranged by size: 3423 1732 993 676 572 366 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6349 6723 6869 387 Size: 1064 138 4760 374 146 1280 Fragments arranged by size: 4760 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 2295 2574 2743 3092 3201 3722 Size: 393 591 72 279 169 349 109 521 Cuts at: 3722 4377 4707 5825 6422 7209 7477 1239 Size: 655 330 1118 597 787 268 1524 Fragments arranged by size: 1524 1118 787 655 597 591 521 393 349 330 279 268 169 109 72 EcoNI CCTnn'n_nnAGG Cuts at: 2761 4131 2761 Size: 1370 6392 EcoRI G'AATT_C Cuts at: 5071 5449 5071 Size: 378 7384 EcoRV GAT'ATC Cuts at: 2176 2176 Size: 7762 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2630 2794 2630 Size: 164 7598 FspI TGC'GCA Cuts at: 4028 6268 4028 Size: 2240 5522 HaeII r_GCGC'y Cuts at: 1678 6909 1678 Size: 5231 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3374 3406 6403 6435 6477 Size: 32 135 32 1802 32 2997 32 42 Cuts at: 6477 6509 1373 Size: 32 2626 Fragments arranged by size: 2997 2626 1802 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2693 2754 3145 4297 5106 5352 Size: 988 281 755 61 391 1152 809 246 Cuts at: 5352 669 Size: 3079 Fragments arranged by size: 3079 1152 988 809 755 391 281 246 61 HindIII A'AGCT_T Cuts at: 748 3916 748 Size: 3168 4594 HpaI GTT'AAC Cuts at: 5352 5352 Size: 7762 KpnI G_GTAC'C Cuts at: 5087 5087 Size: 7762 MfeI C'AATT_G Cuts at: 5361 5361 Size: 7762 MluI A'CGCG_T Cuts at: 7638 7638 Size: 7762 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2095 3174 4594 6876 7060 1305 Size: 790 1079 1420 2282 184 2007 Fragments arranged by size: 2282 2007 1420 1079 790 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5940 Fragments arranged by size: 5940 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2781 4096 4833 5720 6079 6238 518 Size: 648 1615 1315 737 887 359 159 2042 Fragments arranged by size: 2042 1615 1315 887 737 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3035 4024 5178 5855 7003 7248 1606 Size: 1429 989 1154 677 1148 245 2120 Fragments arranged by size: 2120 1429 1154 1148 989 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7210 NdeI CA'TA_TG Cuts at: 387 387 Size: 7762 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7762 NotI GC'GGCC_GC Cuts at: 5209 5209 Size: 7762 NspI r_CATG'y Cuts at: 1132 1229 3143 3355 4477 5120 6665 1132 Size: 97 1914 212 1122 643 1545 2229 Fragments arranged by size: 2229 1914 1545 1122 643 212 97 PasI CC'CwG_GG Cuts at: 1348 4522 1348 Size: 3174 4588 PciI A'CATG_T Cuts at: 3351 6661 3351 Size: 3310 4452 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7762 PmeI GTTT'AAAC Cuts at: 5066 5066 Size: 7762 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5831 5863 7344 7376 5831 Size: 32 1481 32 6217 Fragments arranged by size: 6217 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7762 PsiI TTA'TAA Cuts at: 2834 2976 5332 2834 Size: 142 2356 5264 Fragments arranged by size: 5264 2356 142 PspOMI G'GGCC_C Cuts at: 2148 2148 Size: 7762 PstI C_TGCA'G Cuts at: 830 2409 3021 5114 830 Size: 1579 612 2093 3478 Fragments arranged by size: 3478 2093 1579 612 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7762 PvuII CAG'CTG Cuts at: 3035 4024 3035 Size: 989 6773 SalI G'TCGA_C Cuts at: 1936 3143 5104 1936 Size: 1207 1961 4594 Fragments arranged by size: 4594 1961 1207 SapI GCTCTTCn'nnn_ Cuts at: 2967 2967 Size: 7762 SbfI CC_TGCA'GG Cuts at: 5114 5114 Size: 7762 ScaI AGT'ACT Cuts at: 2304 3768 6010 7722 2304 Size: 1464 2242 1712 2344 Fragments arranged by size: 2344 2242 1712 1464 SfcI C'TryA_G Cuts at: 826 1045 2405 3017 4858 5110 6245 6926 7117 Size: 219 1360 612 1841 252 1135 681 191 Cuts at: 7117 826 Size: 1471 Fragments arranged by size: 1841 1471 1360 1135 681 612 252 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7762 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7762 SmaI CCC'GGG Cuts at: 1872 5089 1872 Size: 3217 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2615 2779 4678 5866 6767 7029 Size: 197 926 672 164 1899 1188 901 262 Cuts at: 7029 820 Size: 1553 Fragments arranged by size: 1899 1553 1188 926 901 672 262 197 164 SnaBI TAC'GTA Cuts at: 493 5553 493 Size: 5060 2702 SpeI A'CTAG_T Cuts at: 152 4082 152 Size: 3930 3832 SphI G_CATG'C Cuts at: 1132 5120 1132 Size: 3988 3774 SspI AAT'ATT Cuts at: 5 52 3277 3310 3655 3860 5686 5 Size: 47 3225 33 345 205 1826 2081 Fragments arranged by size: 3225 2081 1826 345 205 47 33 StuI AGG'CCT Cuts at: 1517 1517 Size: 7762 StyI C'CwwG_G Cuts at: 513 1065 2769 4308 4442 5201 513 Size: 552 1704 1539 134 759 3074 Fragments arranged by size: 3074 1704 1539 759 552 134 SwaI ATTT'AAAT Cuts at: 3864 3864 Size: 7762 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5789 5806 5959 6144 6589 325 Size: 1255 4209 17 153 185 445 1498 Fragments arranged by size: 4209 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2302 3348 3766 Size: 274 80 33 51 1443 325 1046 418 Cuts at: 3766 3808 3819 6008 7720 96 Size: 42 11 2189 1712 138 Fragments arranged by size: 2189 1712 1443 1046 418 325 274 138 80 51 42 33 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3188 3220 3878 3910 7722 7754 3188 Size: 32 658 32 3812 32 3196 Fragments arranged by size: 3812 3196 658 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3679 1316 Size: 2363 5399 XbaI T'CTAG_A Cuts at: 2027 5098 2027 Size: 3071 4691 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2227 2227 Size: 7762 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7762 XmaI C'CCGG_G Cuts at: 1870 5087 1870 Size: 3217 4545 XmnI GAAnn'nnTTC Cuts at: 4100 5891 4100 Size: 1791 5971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5570 276 Size: 53 83 186 936 4036 2468 Fragments arranged by size: 4036 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI