(Linear) (Six-base) MAPSORT of: KIEE3345.seq Check: 5990 from: 1 to: 3057 KIEE3345.orf With 182 enzymes: SgfI * March 22, 2012 16:30 .. AccI GT'mk_AC Cuts at: 0 1139 3057 Size: 1139 1918 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 218 290 569 738 1087 2372 3057 Size: 218 72 279 169 349 1285 685 Fragments arranged by size: 1285 685 349 279 218 169 72 AflII C'TTAA_G Cuts at: 0 610 774 3057 Size: 610 164 2283 Fragments arranged by size: 2283 610 164 AflIII A'CryG_T Cuts at: 0 1346 3057 Size: 1346 1711 AleI CACnn'nnGTG Cuts at: 0 776 3057 Size: 776 2281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 648 3057 Size: 648 2409 AlwNI CAG_nnn'CTG Cuts at: 0 915 1011 1033 1341 2523 3057 Size: 915 96 22 308 1182 534 Fragments arranged by size: 1182 915 534 308 96 22 ApaI G_GGCC'C Cuts at: 0 147 3057 Size: 147 2910 ApoI r'AATT_y Cuts at: 0 359 386 1467 1489 2042 2478 3057 Size: 359 27 1081 22 553 436 579 Fragments arranged by size: 1081 579 553 436 359 27 22 AvrII C'CTAG_G Cuts at: 0 2437 3057 Size: 2437 620 BamHI G'GATC_C Cuts at: 0 678 3057 Size: 678 2379 BanII G_rGCy'C Cuts at: 0 147 2204 3057 Size: 147 2057 853 Fragments arranged by size: 2057 853 147 BbsI GAAGACnn'nnnn_ Cuts at: 0 1075 1640 1675 1906 3031 3057 Size: 1075 565 35 231 1125 26 Fragments arranged by size: 1125 1075 565 231 35 26 BclI T'GATC_A Cuts at: 0 456 477 1713 3057 Size: 456 21 1236 1344 Fragments arranged by size: 1344 1236 456 21 BglI GCCn_nnn'nGGC Cuts at: 0 768 3057 Size: 768 2289 BglII A'GATC_T Cuts at: 0 2184 3057 Size: 2184 873 BlpI GC'TnA_GC Cuts at: 0 2198 3057 Size: 2198 859 Bme1580I G_kGCm'C Cuts at: 0 147 487 773 3057 Size: 147 340 286 2284 Fragments arranged by size: 2284 340 286 147 BmrI ACTGGGnnnn_n' Cuts at: 0 465 1865 3057 Size: 465 1400 1192 Fragments arranged by size: 1400 1192 465 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1196 1717 2702 3057 Size: 1196 521 985 355 Fragments arranged by size: 1196 985 521 355 Bpu10I CC'TnA_GC Cuts at: 0 569 1262 2102 3057 Size: 569 693 840 955 Fragments arranged by size: 955 840 693 569 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2694 3057 Size: 2694 363 BsaI GGTCTCn'nnnn_ Cuts at: 0 2164 3057 Size: 2164 893 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 388 418 3057 Size: 388 30 2639 Fragments arranged by size: 2639 388 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1534 2839 3057 Size: 1534 1305 218 Fragments arranged by size: 1534 1305 218 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1543 3057 Size: 1543 1514 BsmI GAATG_Cn' Cuts at: 0 2457 3057 Size: 2457 600 Bsp1286I G_dGCh'C Cuts at: 0 147 487 773 2204 3057 Size: 147 340 286 1431 853 Fragments arranged by size: 1431 853 340 286 147 BspHI T'CATG_A Cuts at: 0 173 2049 2501 3057 Size: 173 1876 452 556 Fragments arranged by size: 1876 556 452 173 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2718 3057 Size: 2718 339 BsrDI GCAATG_nn' Cuts at: 0 1408 1646 1793 2530 3057 Size: 1408 238 147 737 527 Fragments arranged by size: 1408 737 527 238 147 BsrGI T'GTAC_A Cuts at: 0 1343 3057 Size: 1343 1714 BstAPI GCAn_nnn'nTGC Cuts at: 0 2532 3057 Size: 2532 525 BstYI r'GATC_y Cuts at: 0 678 2184 3057 Size: 678 1506 873 Fragments arranged by size: 1506 873 678 BtsI GCAGTG_nn' Cuts at: 0 591 1445 3057 Size: 591 854 1612 Fragments arranged by size: 1612 854 591 ClaI AT'CG_AT Cuts at: 0 369 3057 Size: 369 2688 DraI TTT'AAA Cuts at: 0 1836 1859 2477 2495 2588 2900 3057 Size: 1836 23 618 18 93 312 157 Fragments arranged by size: 1836 618 312 157 93 23 18 DrdI GACnn_nn'nnGTC Cuts at: 0 1147 3057 Size: 1147 1910 EarI CTCTTCn'nnn_ Cuts at: 0 24 596 962 1955 3057 Size: 24 572 366 993 1102 Fragments arranged by size: 1102 993 572 366 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 218 290 569 738 1087 1196 1717 2372 Size: 218 72 279 169 349 109 521 655 Cuts at: 2372 2702 3057 Size: 330 355 Fragments arranged by size: 655 521 355 349 330 279 218 169 109 72 EcoNI CCTnn'n_nnAGG Cuts at: 0 756 2126 3057 Size: 756 1370 931 Fragments arranged by size: 1370 931 756 EcoRV GAT'ATC Cuts at: 0 171 3057 Size: 171 2886 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 625 789 3057 Size: 625 164 2268 Fragments arranged by size: 2268 625 164 FspI TGC'GCA Cuts at: 0 2023 3057 Size: 2023 1034 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1369 1401 3057 Size: 1369 32 1656 Fragments arranged by size: 1656 1369 32 HincII GTy'rAC Cuts at: 0 688 749 1140 2292 3057 Size: 688 61 391 1152 765 Fragments arranged by size: 1152 765 688 391 61 HindIII A'AGCT_T Cuts at: 0 1911 3057 Size: 1911 1146 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 90 1169 2589 3057 Size: 90 1079 1420 468 Fragments arranged by size: 1420 1079 468 90 MslI CAynn'nnrTG Cuts at: 0 776 2091 2828 3057 Size: 776 1315 737 229 Fragments arranged by size: 1315 776 737 229 MspA1I CmG'CkG Cuts at: 0 1030 2019 3057 Size: 1030 989 1038 Fragments arranged by size: 1038 1030 989 NspI r_CATG'y Cuts at: 0 1138 1350 2472 3057 Size: 1138 212 1122 585 Fragments arranged by size: 1138 1122 585 212 PasI CC'CwG_GG Cuts at: 0 2517 3057 Size: 2517 540 PciI A'CATG_T Cuts at: 0 1346 3057 Size: 1346 1711 PsiI TTA'TAA Cuts at: 0 829 971 3057 Size: 829 142 2086 Fragments arranged by size: 2086 829 142 PspOMI G'GGCC_C Cuts at: 0 143 3057 Size: 143 2914 PstI C_TGCA'G Cuts at: 0 404 1016 3057 Size: 404 612 2041 Fragments arranged by size: 2041 612 404 PvuII CAG'CTG Cuts at: 0 1030 2019 3057 Size: 1030 989 1038 Fragments arranged by size: 1038 1030 989 SalI G'TCGA_C Cuts at: 0 1138 3057 Size: 1138 1919 SapI GCTCTTCn'nnn_ Cuts at: 0 962 3057 Size: 962 2095 ScaI AGT'ACT Cuts at: 0 299 1763 3057 Size: 299 1464 1294 Fragments arranged by size: 1464 1294 299 SfcI C'TryA_G Cuts at: 0 400 1012 2853 3057 Size: 400 612 1841 204 Fragments arranged by size: 1841 612 400 204 SmlI C'TyrA_G Cuts at: 0 610 774 2673 3057 Size: 610 164 1899 384 Fragments arranged by size: 1899 610 384 164 SpeI A'CTAG_T Cuts at: 0 2077 3057 Size: 2077 980 SspI AAT'ATT Cuts at: 0 1272 1305 1650 1855 3057 Size: 1272 33 345 205 1202 Fragments arranged by size: 1272 1202 345 205 33 StyI C'CwwG_G Cuts at: 0 764 2303 2437 3057 Size: 764 1539 134 620 Fragments arranged by size: 1539 764 620 134 SwaI ATTT'AAAT Cuts at: 0 1859 3057 Size: 1859 1198 TatI w'GTAC_w Cuts at: 0 297 1343 1761 1803 1814 3057 Size: 297 1046 418 42 11 1243 Fragments arranged by size: 1243 1046 418 297 42 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1183 1215 1873 1905 3057 Size: 1183 32 658 32 1152 Fragments arranged by size: 1183 1152 658 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1674 3057 Size: 1674 1383 XbaI T'CTAG_A Cuts at: 0 22 3057 Size: 22 3035 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 222 3057 Size: 222 2835 XmnI GAAnn'nnTTC Cuts at: 0 2095 3057 Size: 2095 962 Enzymes that do cut: AccI AcuI AflII AflIII AleI AlfI AlwNI ApaI ApoI AvrII BamHI BanII BbsI BclI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstYI BtsI ClaI DraI DrdI EarI Eco57MI EcoNI EcoRV FalI FspI Hin4I HincII HindIII MmeI MslI MspA1I NspI PasI PciI PsiI PspOMI PstI PvuII SalI SapI ScaI SfcI SmlI SpeI SspI StyI SwaI TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AhdI AloI ApaLI AscI AseI AsiSI AvaI BaeI BanI BbeI BbvCI BcgI BciVI BfrBI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI CspCI DraIII EaeI EagI EciI EcoICRI EcoO109I EcoRI FseI FspAI HaeII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI RsrII SacI SacII SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI TaqII XhoI XmaI ZraI